• 한국생활과학회지
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• 제17권6호
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• pp.1135-1149
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• 2008

The main purpose of this research was to examine a causal model concerning the direct and the indirect effect of family-of-origin functioning on psychological well-being through self-differentiation. The gender differences in the levels of the variables and the correlations among the variables were also examined. Participants were 587 female and 268 male students enrolled in nine different universities. The causal model was tested through structural equation model by using AMOS 8.0 program. The result showed that the levels of self-differentiation and psychological well-being were higher for males than for females, and that the three variables were positively associated to each other for both males and females. The result also indicated that male students' perception of family-of-origin functioning directly influenced their psychological well-being as well as indirectly through self-differentiation, while females' family-of-origin functioning had only a direct effect on their well-being. Results were discussed in terms of the applicability of self-differentiation to Korean college students and of the importance of family-of-origin functioning to improve students' mental health.

• Journal of Ginseng Research
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• 제16권1호
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• pp.31-36
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• 1992

The ultrastructural changes and differentiation mechanism of chromoplasts and leucoplasts from Proplastids in root tip cells of Panax ginseng seedlings were studied with transmission electron microscope. Initial cells have so many proplastids with a few osmiophilic droplets and a lot of mitochon dria at early stage of germination, therefore electron density of cytoplasm is generally higher than that of the other cells just like periblem, plerome and root cap. Proplastids are observed in the initial cells, but only leucoplasts appeared in the central root cap cells. Because root cap cells are derived ultimately from initial cells, the cell organelles in the root cap cells are directly related by those of initial cells. This result postulates that leucoplast is diferentiated from proplastid, and this is the same with other's concepts. On the contrary, the precise observations of chromoplast with crystalline inclusions in the peripheral root cap cells can conclude the direct pathway of chromoplast development from proplastid. Because of the differences of these result from those of other experiments, new scheme of plastid development, direct differentiation of chromoplast from proplastid, can be postulated. And this is the originality of this research on the differentiation of plastids.

• 대한가정학회지
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• 제49권7호
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• pp.13-24
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• 2011

The purpose of this study was to investigate the effects of family-of-origin differentiation, anxiety, and sense of well-being on marital adjustment among married men and women. The Participants of this study were 244 married men and 324 married women, who had preschooler children. The results were as follows: First, family-of-origin differentiation in both men and women had a direct effect on anxiety and sense of well-being. Second, family-of-origin differentiation for both men and women were indirectly influenced by marital adjustment through anxiety and sense of well-being. Third, anxiety of women influenced dyadic consensus at greater degree than men's. Fourth, anxiety and sense of well-being in both men and women had a direct effect on dyadic consensus, dyadic satisfaction, dyadic cohesion and dyadic affection expression. Finally, in women's cases, it was indicated that emotional cut-off from mother directly influences marital adjustment.

• Molecules and Cells
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• 제44권10호
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• pp.723-735
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• 2021

Spemann organizer is a center of dorsal mesoderm and itself retains the mesoderm character, but it has a stimulatory role for neighboring ectoderm cells in becoming neuroectoderm in gastrula embryos. Goosecoid (Gsc) overexpression in ventral region promotes secondary axis formation including neural tissues, but the role of gsc in neural specification could be indirect. We examined the neural inhibitory and stimulatory roles of gsc in the same cell and neighboring cells contexts. In the animal cap explant system, Gsc overexpression inhibited expression of neural specific genes including foxd4l1.1, zic3, ncam, and neurod. Genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) and promoter analysis of early neural genes of foxd4l1.1 and zic3 were performed to show that the neural inhibitory mode of gsc was direct. Site-directed mutagenesis and serially deleted construct studies of foxd4l1.1 promoter revealed that Gsc directly binds within the foxd4l1.1 promoter to repress its expression. Conjugation assay of animal cap explants was also performed to demonstrate an indirect neural stimulatory role for gsc. The genes for secretory molecules, Chordin and Noggin, were up-regulated in gsc injected cells with the neural fate only achieved in gsc uninjected neighboring cells. These experiments suggested that gsc regulates neuroectoderm formation negatively when expressed in the same cell and positively in neighboring cells via soluble factors. One is a direct suppressive circuit of neural genes in gsc expressing mesoderm cells and the other is an indirect stimulatory circuit for neurogenesis in neighboring ectoderm cells via secreted BMP antagonizers.

• 한국철도학회논문집
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• 제5권1호
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• pp.40-47
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• 2002

In this study, and analytical method for ride sensitivity analysis of a train with non-linear suspension elements are proposed. Non-linear characteristics of springs and dampers for primary and secondary suspensions of a train are parameterized using polynomial interpolation. Vertical dynamic model of a three-body train running on straight rail with the predetermined roughness expressed in terms of spectral density function is set up and its equations of motion for ride analysis are derived. Using the direct differentiation method, sensitivity equations of the vertical dynamic model with respect to design parameters associated with non-linearity of suspensions are obtained. Based on the sensitivity analysis, improvement of ride is achieved by varying appropriate suspension parameters.

• 대한기계학회논문집A
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• 제20권5호
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• pp.1458-1467
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• 1996

A direct differentiationmethod is presented for the shape design sensitivity analysis of axisymmeetric elastic solids. Based on the exisymmetric boundary integralequaiton formulation, a new boundary ntegral equatio for sensitivity analysis is derived by taking meterial derivative to the same integral identity that was used in the adjoint variable melthod. Numerical implementation is performed to show the applicaiton of the theoretical formulation. For a simple example with analytic solution, the sensitivities by present method are compared with analytic sensitivities. As an application to the shape optimization, an optimal shape of a gas turbine disc toinimize the weight under stress constraints is found by incorporating the sensitivity analysis algorithm in an optimizatio program.

• 국제지역연구
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• 제16권1호
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• pp.327-349
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• 2012

본 연구의 연구목적은 경제자유구역내 다국적기업의 전략적 외국인직접투자 마케팅과 성과에 관해서 분석하였다. 전략적 외국인직접투자 마케팅 요인은 유치해야할 산업/시장 세그먼트와 유치국 경쟁우위 변수로 측정되었다. 사업성과는 마케팅 차별화 성과, 투자유치 홍보성과, 투자 인센티브 성과, 사업지원 성과 변수로 측정되었다. 본 연구를 위해서 전체 120개의 외국인직접투자 사례의 자료를 확보하여 다음과 같은 연구결과를 얻었다. 첫째, 유치국 경쟁우위와 유치해야할 산업/시장 세그먼트에 따라 국제마케팅유형은 4개의 세그먼트로 유의하게 분류되었다. 둘째, 국제마케팅 유형 중에서 세그먼트4 는 국제마케팅 성과에 대해서 가장 높은 유의한 차이가 나타났다. 셋째, 외국인직접투자 마케팅의 성과 중에서 마케팅 차별화 성과가 가장 높게 나타난 반면에, 사업지원 성과는 가장 낮게 나타났다. 마지막으로, 전략적 외국인직접투자 마케팅의 성과는 마케팅 차별화 성과, 투자유치 홍보성과, 투자 인센티브 성과, 사업지원 성과 순으로 유의한 차이가 나타났다. 본 연구는 경제자유구역내 다국적기업의 전략적 외국인직접투자마케팅과 성과에 대해서 제한적으로 논의를 전개하였으나, 향후의 연구에서는 본 연구의 한계와 제안점을 고려하여 연구에 반영하여야 하겠다.

• International Journal of Oral Biology
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• 제37권1호
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• pp.31-36
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• 2012

Dlx3 and Dlx5 are homeobox domain proteins and are well-known regulators of osteoblastic differentiation. Since possible reciprocal relationships between osteogenic and adipogenic differentiation in mesenchymal stem cells exist, we examined the regulatory role of Dlx3 and Dlx5 on adipogenic differentiation using human dental pulp stem cells. Over-expression of Dlx3 and Dlx5 stimulated osteogenic differentiation but inhibited adipogenic differentiation of human dental pulp stem cells. Dlx3 and Dlx5 suppressed the expression of adipogenic marker genes such as $C/EBP{\alpha}$, $PPAR{\gamma}$, aP2 and lipoprotein lipase. Adipogenic stimuli suppressed the mRNA levels of Dlx3 and Dlx5, whereas osteogenic stimuli enhanced the expression of Dlx3 and Dlx5 in 3T3-L1 preadipocytes. These results suggest that Dlx3 and Dlx5 exert a stimulatory effect on osteogenic differentiation of stem cells through the inhibition of adipogenic differentiation as well as direct stimulation.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.143-151
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• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• 한국발생생물학회지:발생과생식
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• 제13권3호
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• pp.155-161
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• 2009

It has been reported that ganglioside GT1b is expressed during neuronal cell differentiation from undifferentiated mouse embryonic stem cells (mESCs), which suggests that ganglioside GT1b has a direct effect on neuronal cell differentiation. Therefore, this study was conducted to evaluate the effect of exogenous addition of ganglioside GT1b to an in vitro model of neuronal cell differentiation from undifferentiated mESCs. The results revealed that a significant increase in the expression of ganglioside GT1b occurred during neuronal differentiation of undifferentiated mESCs. Next, we evaluated the effect of retinoic acid (RA) on GT1b-treated undifferentiated mESCs, which was found to lead to increased neuronal differentiation. Taken together, the results of this study suggest that ganglioside GT1b plays a crucial role in neuronal differentiation of mESCs.