• Journal of Aquaculture
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• v.8 no.3
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• pp.231-240
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• 1995

This study was conducted to examine early gonadogenesis by using a histological method for the appearance of primordial germ cells (PGCs), protrude of genital ridge, and formation of primitive gonads in diploid and triploid mud loach, Misgurnus mizolepis. The pattern of early gonadogenesis including appearance of PGCs, formation of genital ridge, and development of primitive gonad in both diploid and triploid were not different histologically. Characteristics of PGCs of triploid were also the same as those of diploid. However, gonadal length of diploid was significantly longer than that of triploid (P<0.05).

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.53-53
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• 2003

The aim of this study was to determine the interactive effects of BSA and EGF on the viability and development of porcine diploid parthenotes developing in vitro. The addition of 0.1 and 0.4% BSA to the culture medium enhanced the development of 4-cell parthenotes to the blastocyst stage but EGF had no effect. However, while BSA also increased cell numbers, it did so only when EGF was also present. Either agent on its own had no effect. Similarly, apoptosis in the blastocysts was not influenced by either agent on its own but was reduced when both BSA and EGF were present. Furthermore, semi-quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) revealed that EGF enhanced the mRNA expression of BclxL in the presence of 0.4% BSA but BSA and EGF alone had no effect. EGF and/or BSA did not influence Bak gene expression in the blastocyst stage parthenotes. These results suggest that BSA has both beneficial and detrimental effects on the viability of porcine diploid parthenotes developing in vitro and that exogenous EGF may block some of the detrimental effects of BSA, possibly by inhibiting the BSA-induced apoptosis by increasing Bcl-xL expression. This results in a net increase in cell numbers in porcine diploid parthenotes developing in vitro.

• Journal of applied mathematics & informatics
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• v.31 no.1_2
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• pp.241-246
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• 2013

Choi [1] identified and characterized the limiting diffusion of this diploid model by defining discrete generator for the rescaled Markov chain. In this note, we define the operator of projection $S_t$ on limiting diffusion and new measure $dQ=S_tdP$. We show the martingale property on this operator and measure. Also we conclude that the martingale problem for diffusion operator of projection is well-posed.

• Journal of applied mathematics & informatics
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• v.29 no.3_4
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• pp.1043-1048
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• 2011

W. Choi([1]) identified and characterized the limiting diffusion of this diploid model by defining discrete generator for the rescaled Markov chain. We denote by F the homozygosity and by S the average selection intensity. In this note, we define the Fleming-Viot process with generator of limiting diffusion and provide exact result for the relations of F and S.

• Bulletin of the Korean Mathematical Society
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• v.42 no.2
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• pp.397-404
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• 2005

In this note, we characterize the limiting diffusion of a diploid model by defining the discrete generator for the resealed Markov chain. We conclude that this limiting diffusion model is with uncountable state space and mutation selection and special 'mutation or gene conversion rate'.

• Journal of Plant Biology
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• v.14 no.1
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• pp.33-35
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• 1971

After topping, axillary buds of haploid plants derived from cultured anthers were treated with 0.4% aqueous solution of colchicine. Due to the high temperature and dry air at the time of treatment, most of the buds perished. A few months after the colchicine application, however, several shoots arose from the places where the dead buds were originally located. These shoots were mostly diploid. Induction of adventive shoots from the colchicine-treatedaxils was supposed to be rather effective method of obtaining diploid shoots from haploid plants. The diploid plants had larger floral organs than the haploid plants, and had good pollen fertility and seed setting. 24 bivalent chromosomes were observed at MI of the PMC's.

• Journal of Marine Life Science
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• v.3 no.1
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• pp.9-21
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• 2018

This study investigated cytogenetic and hematological and histological characteristics between diploid and spontaneous triploid on Cyprinidae (Crucian carp, Carassius auratus; crucian carp, C. cuvieri and common carp, Cyprinus carpio) in four major rivers of Korea. In our results, DNA contents of triploid Cyprinidae were 50% more than those of diploid Cyprinidae. Also, erythrocyte size of triploid Cyprinidae was 50% larger than those of diploid Cyprinidae. In all sampling rivers, sex ratios of C. auratus were biased to female, and especially, triploid groups of C. auratus were all female groups (p<0.05). In principle, sex ratios of C. cuvieri and common carp were equivalent between male and female.

• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.333-339
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• 1994

Enzyme activities and phenolic compound were compared to investigate the physiological characteristics during shoot formation from diploid and haploid of Nicotiana tabacum w BY4. The Nakata medium with 1.0 mg/L IAA, 0.5 mg/L Kinetin and 3 g/L activie carbon was excellent to induce the haploid plants from the middle size anther within 30 days after culture. The MS medium with 0.5 mg/L 2,4-D was good for callus induction from leaf explants of diploid and haploid, and a lot of plane were regenerated from calli on the MS medium supplemented with 2.0 mg/L BAE Activities of peroxidase for both of diploid and haploid plane were the highest at 2.0mg/L BAP Activities of IAA oxidase and catalase of haploid Plants were high or than those of diploid plants. On the other hand, activity of peroxidase of haploid plants were lower than those of diploid plants.

• Korean Journal of Plant Taxonomy
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• v.34 no.2
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• pp.153-166
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• 2004

Genus Taraxacum propagated through diploid sexual reproduction and polyploid agamospermy. The cluster analysis of Korean(2 species, 4 population), Japanese (2 species 4 taxa 6 population) and Taiwanese (1 species, 3 population) Taraxacum species using 15 measured capitulum morphological characters was conducted to study the speciation of diploid Taraxacum in East Asia. We measured 15 capitulum morphological characters including length of capitulum, length and shape of outer-involucre, corniculate appandage. Within one population, these characters were very various and were overlapped. The result of cluster analysis using morphological character showed that all species were clustered into four groups (Korean species & T. japonicum group, T. platycarpum subspecies group, T. platycarpum subsp. hodense group, T. formosanum group). Korean species, T. hallaisanense (diploid) and T. ohiwanum (diploid, triploid) were clustered into T. japonicum that was occurred in Kansai provinces of Japan. Therefore, we could infer that Korean species was closely related to Japanese T. japonicum of Japanese species.

• Korean Journal of Veterinary Research
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• v.30 no.3
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• pp.317-331
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• 1990

Chromosomes of gonadal tissues from Fasciola spp, Eurytrema pancreaticum and Calicophoron calicophorum occurred Korean cattle were egamined using modified air-drying method. To compare their phenotype with three different genotypes among Fasciola spp, the adult and egg si2e were measured since they have been known as important taxonomical characters. The results obtained were as followed; Cattle liver fluke, Fasciola spp were classified into three types based on their chromosomal complements such as individual with 2o chromosome(diploid), 30 chromosome(triploid) and 20/30 mosaic constitution(mixoploid). The propotions of appearance of three types were 40.00%, 54.29% and 5.71%, respectively. The frequency of three types in type I which was regarded as F gigantica were 58.82% for diploid, 35.29% for triploid and 5.88% for mixoploid, but in type II which was regarded as F hepatica were 72.2% for triploid, 22.22% for diploid and 5.56% for mixoploid. Egg length of triploid forms was significantly larger than that of diploid forms and egg size of mixoploid forms was similiar to that of triploid forms. Worm size of triploid forms was larger than that of diploid forms and was more similar to that of mixoploid forms, but the statistical data were not significant. Diploid chromosome consisted of one pair of metacentric chromosome(m), four pairs of submetacentric chromosomes(sm), five pairs of subtelocentric chromosomes(st), while triploid chromosome consisted of one pair of metacentric chromosome, seven pairs of submetacen.tric chromosomes, one pair of subtelocentric chromosome and telocentric chromosome(t), respectively. In mixoploid chromosome, constitution of the chromosomes of diploid or triploid cell was consistent with that of diploid or triploid. Chromosomes of gonadal tissues from pancreatic fluke, Eurytrema pancreaticum consisted of 13 pairs of homologs(2n=26, n=13). The mitotic and meiotic divisions were observed frequently. In the mitotic metaphase, Karyotype consisted of five pairs of metacentric chromosomes, four pairs of submetacentric chromosomes, three pairs of subtelocentric chromosomes and one pair of telocentric chromosome. Chromosomes of gonadal tissues from stomach fluke, Calicvphoron calicophorum consisted of 9 pairs of homologs(2n=18, n=9). The meiotic divisions was frequently observed, but mitotic divisions was rare. In the mitotic metaphase, Karyotype consisted of two pairs of metacentric chromosomes, three pairs of submetacentric chromosomes and four pairs of subtelocentric chromosomes. Karyotype of Calicophoron calicophorum differed from that of Japanese C calicophorum which was similar to that of Paramphistomum cervi of Korean cattle. Though that of Calicophoron calicophorum of Korean cattle was similar to that of Paramphistomum explanatum of Korean cattle, that have been recognized to be a different species of fluke.