• 제목/요약/키워드: dipheny-picryl-hydrazyl (DPPH)

검색결과 3건 처리시간 0.017초

보골지(補骨脂)가 남성 생식세포 GC-1의 항산화에 미치는 영향 (Antioxidant Effects of Psoraleae Fructus in GC-1 Cells)

  • 오명숙;김도림;김소연;장문석;박성규
    • 동의생리병리학회지
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    • 제19권1호
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    • pp.81-86
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    • 2005
  • The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Psoraleae fructus. The extract was studied for dipheny-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being 0.427 mg/mL. The extract was dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (67.7 %) was blocked by the extract concentration- dependently. Furthermore, the extract also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Psoraleae fructus has potent antioxidant activity.

함초를 첨가한 두부의 이화학적 특성 및 항산화성 (Physiochemical Characteristics and Antioxidant Activity of Soybean Curd Added with Saltwort (Salicornia herbacea L.))

  • 신미경;김명희;홍금주
    • 한국식품조리과학회지
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    • 제29권2호
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    • pp.123-127
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    • 2013
  • In this study the physiochemical characteristics and antioxidant activity of soybean curd were prepared with saltwort (Salicornia herbacea L.). The moisture and protein of the saltwort soybean curd showed no difference among the samples. Ash and lipid were found to be the highest in the soybean curd to which 20% saltwort was added. The contents of mineral(Fe, K, Mg, Zn) were highest in the soybean curd to which 20% saltwort was added, and Ca content was high in the soybean curd to which 16% and 20% saltwort was added. DPPH (1,1-dipheny1-2-picryl-hydrazyl) radical scavenging activity is a method of measuring the antioxidant power of food; this was high in the soybean curd to which 20% saltwort was added. Adding more saltwort led to higher scavenging activity. From the above results, it could be seen that the soybean curd, which is made by adding saltwort, contributed to enhancing the functionality of antioxidant activation and product quality.

격강구의 연소과정에서 생성된 격강구진의 항산화 효과 (Antioxidant Effects of the Moxi with Ginger Tar Produced by Moxibustion with Ginger Combustion)

  • 서상록;양승범;김재효;안성훈;손인철
    • Korean Journal of Acupuncture
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    • 제28권4호
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    • pp.29-40
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    • 2011
  • Objectives : This study estimated antioxidant effects of the moxi with ginger tar (MGT) ; chemical combustion products produced by moxibustion with ginger during combustion. Methods : To do this aim, we investigated total polyphenol and flavonoid contents, SOD (superoxide dismutase) scavenging activity, ABTS (2.2-azino-bis-3-erthylbenzo-thiazoline-6- sulfonic acid) & DPPH(2,2-Dipheny1-1 -picryl-hydrazyl) radical's scavenging ability of MGT. Results : Total polyphenol contents of MGT was $7.8{\pm}0.09$ mg/g in 10 mg/ml, SOD activity was $42.51{\pm}3.39%$ in 200 ug/ml, DPPH radical scavenging effect of MGT was $83.24{\pm}0.01%$ in 200 ug/ml and ABTS radical scavenging effect was $41.88{\pm}0.16%$ in 200 ug/ml. Conclusions : In this study, the effects of moxi with ginger could be induced by not only heating stimulus on acupoints but also chemical stimulus produced during combustion of moxibustion, MGF. The advanced study about biological mechanism through meridian system, skin aging, and inflammation on MGT will be required.