• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.60-66
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• 2002

Aryl hydrocarbons, environmental contaminants accumulate in tissue and pose potential risk in human health. 2,3,7,8-Tertachlorodibenzo-p-dioxin (TCDD) is known as a most potent toxicant among aryl hydrocarbons. TCDD elicits numerous toxic responses in experimental animals and human, including hepatic carcinoma, pulmonary and skin tumor in adult rodents, craniofacial abnormality during mouse embryogenesis, chloracne, reproductive abnormality, immunotoxicity, endocrine effects in exposed humans.(omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.90-105
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• 2002

The dioxin- or aryl hydrocarbon receptor (AhR) is a member of the Per-Arnt-Sim family of nuclear transcription factors exhibiting a basic helix-loop-helix structure. In its non-ligated state the AhR is associated with hsp 90 and the immunophilin-type XAP2. Upon ligand binding the associated proteins are released, the receptor dimerizes with the AhR nuclear trans locator protein Arnt, and binds to XREs (xenobiotic-responsive elements) in the 5'-flanking region of responsive genes thus modulating their transcription.(omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.211.2-211.2
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• 2003

Saururus Chinensis Baill (Saururaceae) has been used as folk medicine for analgesics, beriberi, edema, hepatitis, and icterus, etc. Hepatoprotective effects of Saururus chinensis Baill (SCB) administration on function of the biochemical parameters in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats were investigated. After 7 days from TCDD(1$\mu\textrm{g}$/kg) injection, SCB(200mg/kg) was administered into rats intraperitoneally for 4 week.s We examined the antioxidative enzymatic activity by measuring the level of AST and ALT in serum and SOD, Catalase, GPx, GSH and GSSG in liver tissue of rats. (omitted)

• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.164-168
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• 2001

Abel et al. in Germany discovered a new dioxin-responsive gene, which has later been identified as rpt-1 (regulatory protein T-lymphocyte 1). While it is speculated that rpt-1 may play a role in signal transduction and carcinogenesis, its roles and functions remain unknown. The present study attempted to analyze functions of rpt-1 in human epithelial cells following the xenobiotic exposures. While German counterpart analyzed expressionn of rpt-1 in spleen and thymus cells from mouse and rat and characterizes molecular properties of the gene, our work mainly focused on analyzing function of rpt-1 in human skin cells. Expression of rpt-1 in human cells were analyzed by western and northern blot RT-PCR analysis. Expression of rpt-1 as well as Staf-50 in human cells with or without exposure to environmental pollutants were also analyzed by northern blot analysis, since Staf-50 is homologous with rpt-1 and found in human cells. To help study roles of rpt-1 in human cell system, retroviral vector system carrying rpt-1 gene under the CMV promoter were constructed and transfected. Cells overexpressing the gene after the transfection showed an increase of cell density and soft agar colony formations, as compared to the control cells, suggesting that rpt-1 may play a certain role in the transformation processes of human cells. While the expression of rpt-1 in spleen and thymus is known to be strong in the laboratory animals, both the basal and TCDD-induced expression of rpt-1 in the current cellular system remained insignificant. It is speculated that the expression pattern of rpt-1 may be tissue- and species-specific. The present study demonstrated a strong expression of rpt-1 protein in the brain of SD rat model. Since there is no previous report on the expression of rpt-1 in the brain tissue, the result may play a significant role in understanding dioxin-induced neurotoxicities in the future. The present study provides an opportunity to understand a role of rpt-1 in human cell system and suggest a possible lead and basis for the future study of dioxin-induced neurotoxicities.

• 동의생리병리학회지
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• 제16권4호
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• pp.782-787
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• 2002

This study was carried out to investigate the protective effect of chitosan on lipid peroxidation and key lipid parameters in Sprague-Dawley rat (SO rat) accutely exposured to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Male SO rats received single intraperitoneal (ip) injection of TCDD (40 j.lg/kg), and were given diet containing 3 or 5% chitosan for 4 weeks from 1 week before TCDD treatment. The gain in body weight was less in group treated with TCDD than in CON group, while that of Ch/H+ TCDD group (5% chitosan diet) increased. The decrease in liver and testis weight caused by TCDD was prevented by high dietary intake of chitosan (5% chitosan). Serum (total cholesterol, triglyceride, HDL-C, and LDL-C) and liver lipid parameters (total lipid, total cholesterol, and triglyceride) were significantly elevated in TCDD-induced rats, but these parameters excluding HDL-C were significantly reduced in high dietary intake of chitosan (5% chitosan). These findings suggest that chitosan is believed to be a possible protective effect against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat.

• 농약과학회지
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• 제10권1호
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• pp.1-6
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• 2006

고체상 지지체인 4-클로로-3-나이트로벤조페논 옥심 resin 5를 이용하여 카르복스아닐라이드 유도체를 고체상에서 합성할 수 있는 방법을 개발하였다. 4-클로로-3-나이트로벤조페논 resin 6과 하이드록실아민 염산염을 축합반응하여 옥심 resin 5를 얻었다. 옥심 resin 5에 각각 다이옥신 및 옥사티인 유도체 7a-d를 결합하여 상응하는 고체상에 결합된 다이옥신 및 옥사티인 화합물 9a-d를 얻었다. 이 고체상 resin 9a-d를 초산 존재 하에서 아닐린으로 각각 처리하여 상응하는 다이옥신 및 옥사티인 카르복스아닐라이드 유도체 10a-d(수율 5%-정량적)를 합성하였다.

• 생약학회지
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• 제32권2호통권125호
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• pp.121-127
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• 2001

The effect of bear bile on 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD)-induced hepatotoxicity was investigated in 6-week-old C57BL/6 male mice. Bear bile (100 mg/kg or 500 mg/kg) was administered orally daily for 4 weeks, respectively. From the second week, $10\;{\mu}g/kg$ of TCDD was administered to the bear bile-treated animals orally once a week for 3 weeks (a total of $30\;{\mu}g/kg$). There were no specific clinical findings and significant body weight changes in all groups. Although the livers in TCDD-treated mice appeared a severe hypertrophy and many necrotic foci, and changed to yellow-brown color in gross findings, these lesions were remarkably reduced by bear bile administration. The elevated serum activities of alanine transaminase, aspartate transaminase, alkaline phosphatase, and lactate dehydrogenase due to TCDD were significantly decreased by bear bile treatment (P<0.05). The lipid peroxidation induced by TCDD was significantly prevented by bear bile administration (P<0.05). In histological examinations, there were a moderate necrosis of hepatic cells around central veins, severe cytoplasmic vacuolizations, inflammatory cell infiltrations, and remarkable fatty changes in the liver of TCDD-treated animals. However, the lesions were dose-dependently inhibited by the bear bile treatments. These findings indicate that bear bile may have a protective effect against TCDD-induced hepatotoxicity in mice.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2002년도 학술대회
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• pp.123-126
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• 2002

Pollutants that are persistent, bioaccurnulative, and toxic have been linked to numerous adverse effects in human and animals, PBTs include heavy metals, polychlorinated biphenyls (PCBs), dioxins, polycyclic aromatic compounds (PACs) in addition to pesticides. This study focuses on toxic effects of the PBTs except pesticides on insects. Eight PBTs were selected from subgroups: three heavy metals (Pb, Hg, and Cd), two PCB mixtures (Aroclor mixtures 1 and 2), 2,3,7,8-tetrachlorodibenzo-p-dioxin, two monophenols (4-octylphenol and 4-nonylphenol), and tetrabutyltin, Beet armyworm, Spodoptera exigua, was used as test target insect species. Three physiological markers (metamorphosis, immune reaction, and follicle patency) were assessed in each exposure to different doses of the PCBs. Heat-shock proteins as molecular markers were also analyzed in response to the PCBs. All tested PBTs were toxic to metamorphosis from larvae to pupae when they were applied with diet. Two PCB mixtures were the most toxic compounds in this assay by giving significant toxicity at 0.005 ppm, while others had from 10 to 1000 ppm. Dioxin (0.1 ppb), tetrabutyltin (0.1 ppb), Pb (10 ppb), and Hg (0,01 ppb) were potent to inhibit immune reactions analyzed by inducing phenoloxidase activity and blocked phospholipase $A_2$ enzyme, Tetrabutyltin and dioxin significantly induced follicle cell patency, but their effects were lower than that of endogenous juvenile hormone, Dioxin, Pb, Hg, and Cd could induce the expression of heat shock proteins that were detected by immunoblotting against human HSP70 monoclonal antibody. HSP78 and HSP80 were upregulated in response to the PBTs. This expression was detected from the fat body and epidermis at as fast as 4h after injection. All these results clearly suggest that PBTs give significant ecotoxicity to insects that are valuable organisms in our environment.

• 대한환경공학회지
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• 제22권1호
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• pp.179-185
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• 2000

도시쓰레기 소각로에서 다이옥신류의 생성과 대기로의 방출을 최소화하기 위해 소각로의 후 연소 영역에서 다이옥신 전구물질의 원인이 되는 클로로페놀(이하 CP로 표기)의 거동에 대해 검토하였다. 전기관상로를 이용하여 온도조건 $300{\sim}500^{\circ}C$ 에서 CP을 주입하고 질소가스를 이용하여 반응시간을 조절하였으며 CP의 이성질체별 연소에 필요한 산소량은 계산된 실험식을 이용하여 주입하였다. 반응기내의 공간속도가 60~80/초의 조건에서 Mo-V계 촉매를 사용하여 촉매유무에 따른 CP의 분해 효율을 살펴보았다. 무촉매 열분해시 mono-CP은 74~80%, di-CP은 55~66%, tri-CP은 50~58%의 효율을 보였고, Mo-V계 촉매가 존재할 때 mono-CP은 90~99.9%, di-CP은 86~97%, tri-CP은 76~99%의 효율을 나타내 Mo-V계 촉매를 사용함으로써 약 20~30%의 효율이 증가함을 확인할 수 있다.

• 분석과학
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• 제17권2호
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• pp.184-191
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• 2004

본 연구에서는 HRGC/HRMS에 의한 다이옥신분석에 있어 가장 널리 사용되고 있는 SP-2331 및 DB-5MS 컬럼에 의한 독성 2,3,7,8-다이옥신 동족체 분리의 영향에 대하여 조사하였다. 컬럼분리성능평가 표준용액과 비산재 시료의 분석을 통하여 이들 컬럼에 의한 tetra, penta 및 hexa-2,3,7,8-동족체의 분리에 대한 부분과 I-TEQ값의 영향을 중심으로 설명하였다. 이들 두 컬럼에 의한 분석에서 컬럼분리성능평가 표준용액의 경우 DB-5MS에 의해서는 총 2,3,7,8-치환이성체의 농도는 508.4 ng/mL로 나타났으며, SP-2331에 의해서는 515.8 ng/mL로 나타났다. 이들을 I-TEQ값으로 환산하였을 때 거의 동등하게 나타났다. 비산재 시료의 경우에서도 이들 두 컬럼에 의한 2,3,7,8-치환이성체의 총 농도와 I-TEQ값이 거의 비슷하게 나타났다.