• 제목/요약/키워드: differentiation.

검색결과 7,891건 처리시간 0.036초

생약의 파골 세포 분화 저해활성 검색 (Inhibitory Activity of Medicinal Plants against Differentiation of Osteoclasts)

  • 이준원
    • 생약학회지
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    • 제40권2호
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    • pp.83-88
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    • 2009
  • Bone is continuously remodeled by osteoblasts and osteoclasts. Osteoclasts play an important role in bone metabolism by resorbing the bone matrix. Thus, the compounds inhibiting osteoclasts can improve bone diseases such as osteoporosis. The methanol extracts of 159 herbal medicines were screened for the inhibitory activity against differentiation of osteoclasts. Among the tested extracts, Achuranthis Radix and Corydalis Tuber showed relatively strong inhibitory activity against differentiation of osteoclasts, whereas they have no significant effect on proliferation of osteoclasts.

STABLE NUMERICAL DIFFERENTIATION: WHEN IS IT POSSIBLE?

  • Ramm, Alexander G.;Smirnova, Alexandra
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • 제7권1호
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    • pp.47-61
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    • 2003
  • Two principally different statements of the problem of stable numerical differentiation are considered. It is analyzed when it is possible in principle to get a stable approximation to the derivative ${\Large f}'$ given noisy data ${\Large f}_{\delta}$. Computational aspects of the problem are discussed and illustrated by examples. These examples show the practical value of the new understanding of the problem of stable differentiation.

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생약으로부터 세포분화유도물질의 검색 및 분리 및 분리 (I) (Screening and Isolation of the Cell Differentiation Inducers from Medicinal Plants (I))

  • 박은정;김진웅
    • 생약학회지
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    • 제28권4호
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    • pp.225-232
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    • 1997
  • 300 extracts derived from 100 plants were tested for their potential to induce HL-60 cell differentiation using NBT assay and NSE/SE staining methods. Morphological changes from suspended to adherent state of the cells were also observed by microscopic examination. In result, 55 extracts induced cell differentiation into monocyte/macrophage lineage in the NBT and the NSE assay.

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HDAC11 Inhibits Myoblast Differentiation through Repression of MyoD-Dependent Transcription

  • Byun, Sang Kyung;An, Tae Hyeon;Son, Min Jeong;Lee, Da Som;Kang, Hyun Sup;Lee, Eun-Woo;Han, Baek Soo;Kim, Won Kon;Bae, Kwang-Hee;Oh, Kyoung-Jin;Lee, Sang Chul
    • Molecules and Cells
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    • 제40권9호
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    • pp.667-676
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    • 2017
  • Abnormal differentiation of muscle is closely associated with aging (sarcopenia) and diseases such as cancer and type II diabetes. Thus, understanding the mechanisms that regulate muscle differentiation will be useful in the treatment and prevention of these conditions. Protein lysine acetylation and methylation are major post-translational modification mechanisms that regulate key cellular processes. In this study, to elucidate the relationship between myogenic differentiation and protein lysine acetylation/methylation, we performed a PCR array of enzymes related to protein lysine acetylation/methylation during C2C12 myoblast differentiation. Our results indicated that the expression pattern of HDAC11 was substantially increased during myoblast differentiation. Furthermore, ectopic expression of HDAC11 completely inhibited myoblast differentiation, concomitant with reduced expression of key myogenic transcription factors. However, the catalytically inactive mutant of HDAC11 (H142/143A) did not impede myoblast differentiation. In addition, wild-type HDAC11, but not the inactive HDAC11 mutant, suppressed MyoD-induced promoter activities of MEF2C and MYOG (Myogenin), and reduced histone acetylation near the E-boxes, the MyoD binding site, of the MEF2C and MYOG promoters. Collectively, our results indicate that HDAC11 would suppress myoblast differentiation via regulation of MyoD-dependent transcription. These findings suggest that HDAC11 is a novel critical target for controlling myoblast differentiation.

거주지역에 따른 결혼이민자 여성의 자아분화 및 문화적응이 결혼만족도에 미치는 영향 (Influence of Self-Differentiation and Acculturation on Marriage Satisfaction Among Immigrant Women by Residential Area)

  • 이영분;이유경
    • 가정과삶의질연구
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    • 제28권1호
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    • pp.145-157
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    • 2010
  • This is a study that explores the influence of self-differentiation and acculturation among married immigrant women on their feelings of marriage satisfaction by residential area. The aim was to verify the level of self-differentiation and acculturation that married immigrant women secure from multicultural marriage. To achieve this objective, the study widely distributed a questionnaire that targeted women who were participating in education and other services at health and family support centers, multi-cultural family support centers, general social welfare centers, immigrant women shelters, and Korean language classrooms which are located in Seoul, Gyeonggi, Chungcheong, Jeolla, and Gyeongsang. Data analysis involved frequency analysis, descriptive statistics, one-way-Anova, and multiple regression analysis. Based on the results of descriptive statistics, two factors, namely, (1)interpersonal-relation differentiation, a sub-scale of self-differentiation, and (2)marginalization, a sub-scale of acculturation, had the lowest average. In verifying its various hypotheses, the study achieved the following results. Firstly, among demographic characteristics, there was difference of the mean in the marriage period, average monthly income, the frequency of meetings with the married woman´s parents-in-law and her own parents, and the average cost of supporting the woman's parents-in-law and her own parents. Secondly, among demographic characteristics, the variable of influencing marriage satisfaction showed negative influence in the case of women dwelling in farming and fishing villages. This shows that women residing in cities whether small, medium or large have higher marriage satisfaction. Also, as a result of verifying whether self-differentiation has influence on marriage satisfaction, the element of interpersonal-relations differentiation had a negative influence on marriage satisfaction. Thirdly, as for influence of acculturation upon marriage satisfaction, the study showed that just integration, which is a sub-scale of acculturation had positive effect on marriage satisfaction. In other words, the study showed that the interpersonal-relation differentiation among the sub-scales of self-differentiation among married immigrant women had a negative influence, and that integration among the sub-scales in acculturation had a positive influence on marriage satisfaction. Based on these results, in order to increase interpersonal-relation differentiation, as well as marriage satisfaction among immigrant women, the study suggests the integration of the women's families with the nuclear and extended families in the communities where the women reside.

리포터유전자를 이용한 조골세포 분화정도에 관한 연구 (A study on the osteoblast differentiation using osteocalcin gene promoter controlling luciferase expression)

  • 김경화;박윤정;이용무;한중석;이동수;이승진;정종평;설양조
    • Journal of Periodontal and Implant Science
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    • 제36권4호
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    • pp.839-847
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    • 2006
  • The aim of this study is to monitor reporter gene expression under osteocalcin gene promoter, using a real-time molecular imaging system, as tool to investigate osteoblast differentiation. The promoter region of mouse osteocalcin gene 2 (mOG2), the best-characterized osteoblast-specific gene, was inserted in promoterless luciferase reporter vector. Expression of reporter gene was confirmed and relationship between the reporter gene expression and osteoblastic differentiation was evaluated. Gene expression according to osteoblstic differentiation on biomaterials, utilizing a real-time molecular imaging system, was monitored. Luciferase was expressed at the only cells transduced with pGL4/mOGP and the level of expression was statistically higher at cells cultured in mineralization medium than cells in growth medium. CCCD camera detected the luciferase expression and was visible differentiation-dependent intensity of luminescence. The cells produced osteocalcin with time-dependent increment in BMP-2 treated cells and there was difference between BMP-2 treated cells and untreated cells at 14days. There was difference at the level of luciferase expression under pGL4/mOGP between BMP-2 treated cells and untreated cells at 3days. CCCD camera detected the luciferase expression at cells transduced with pGL4/mOGP on Ti disc and was visible differentiation-dependent intensity of luminescence This study shows that 1) expression of luciferase is regulated by the mouse OC promoter, 2) the CCCD detection system is a reliable quantitative gene detection tool for the osteoblast differentiation, 3) the dynamics of mouse OC promoter regulation during osteoblast differentiation is achieved in real time and quantitatively on biomaterial. The present system is a very reliable system for monitoring of osteoblast differentiation in real time and may be used for monitoring the effects of growth factors, drug, cytokines and biomaterials on osteoblast differentiation in animal.