• East Asian Journal of Business Economics (EAJBE)
• /
• v.9 no.2
• /
• pp.11-19
• /
• 2021

Purpose - The increase in organizational citizenship behavior is important in that it creates a cooperative atmosphere within the organization. Proving how OCB toward coworkers changes in negative leadership situations such as abusive supervision is a new research stream. Therefore, this study examines the main effects of the supervisor's abusive supervision on OCB of subordinates for coworkers, and then attempts to clarify that the perceived LMX differentiation of team members play a moderating role. Research design, data, and methodology - First, we examine the effects of the supervisor's abusive supervision on OCB of subordinates in the team toward colleagues. Second, the degree of perceived LMX differentiation is measured to verify the moderating effect of abusive supervision on OCB toward coworkers. Result - Hypothesis 1 is that abusive supervision has a negative effect on coworkers' OCB. Hypotheses 2-1, 2-2, and 2-3 test the moderating effect of perceived LMX differentiation, and the relationship between abusive supervision and coworkers' OCB will change according to the perceived degree of LMX differentiation. Conclusion - It is significant in that the scope of abusive supervision research has been expanded through the team level moderator of perceived LMX differentiation, away from the research on abusive supervision that was mainly discussed at the level of dyadic relationship (between the supervisor and the one subordinate).

• Human Ecology Research
• /
• v.60 no.3
• /
• pp.459-470
• /
• 2022

This study analyzed the relationship between adult attachment, self-differentiation, and self-esteem in solving the fear of intimacy in interpersonal relationships experienced by adults. This study targeted people in the 20s to 50s age group, and data from 1,263 participants were analyzed using SPSS 23.0 and AMOS 23.0. The results are as follows. First, it was found that secure attachment had a negative relationship with the fear of intimacy, while the relationship between self-differentiation and self-esteem was positive. On the other hand, anxiety attachment and avoidance attachment were shown to have a positive relationship with the fear of intimacy, but a negative relationship was identified with self-differentiation and self-esteem. Second, it became evident that self-differentiation and self-esteem mediated the relationship between adult attachment and the fear of intimacy. Additionally, the findings indicated that self-differentiation and self-esteem had a dual mediating effect. The results of this study can be used as basic data for counseling and educational programs to solve the fear of intimacy in interpersonal relationships experienced by adults.

• Biomedical Science Letters
• /
• v.29 no.3
• /
• pp.190-200
• /
• 2023

Skeletal muscle, essential for metabolism, thermoregulation, and immunity, undergoes myogenic differentiation that results in myotube formation. Trans-anethole (TA), the major constituent in essential oil produced by anise, star anise, and fennel, whose function in skeletal muscle has not yet been elucidated. Therefore, we investigated whether TA influenced muscle differentiation in mouse C2C12 myoblasts. Cells were induced to differentiate using a differentiation medium with or without TA (50 or 200 mg/mL) daily for 5 days. We measured myotube length and diameter after differentiation days 1, 3, and 5 and analyzed the expression of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain) and atrophy-related genes (atrogin-1 and muscle ring finger-1 [MuRF-1]) using quantitative real-time PCR. Additionally, we observed the expression of total protein kinase B (Akt) and phosphorylated Akt (p-Akt) using western blotting. Our data showed that TA significantly induced the formation of smaller and thinner myotubes and reduced the myogenic factor expression. Furthermore, the atrogin-1 and MuRF-1 expression markedly increased by TA. Consistent with these findings, TA significantly decreased the expression of total Akt and p-Akt. Taken together, these results indicate that TA inhibits myogenic differentiation of C2C12 cells via reduction of both total Akt and p-Akt. Our findings may provide valuable insights into the impact of PAA on individuals at risk of muscle atrophy.

• International Commerce and Information Review
• /
• v.14 no.3
• /
• pp.113-138
• /
• 2012

This study examined the determinants of export performance of small and medium sized manufacturing companies in Korea. Depending on the existing research, taking entrepreneurship and product differentiation capabilities as antecedents of export performance. In addition, the product differentiation capabilities examined whether the role of the mediating effects between entrepreneurship and export performance. Thus, the purpose of the study is look at the integrated model of entrepreneurship, product differentiation capabilities and export performance building and their relationship. On 152 domestic companies, empirical analysis was performed. Empirical analysis was conducted using the PLS(Partial Least Square). And analysis tools were used SmartPLS2.0. In the results of the analysis, risk tolerance and innovativeness of small and medium sized manufacturing businesses, entrepreneurs and product differentiation competence have positive impact export performance in both. Product differentiation capabilities also confirmed that it have a positive impact on the export performance of small manufacturing export enterprises. In analysis of the mediated effect in product differentiation capacity showed a significant mediated effect between innovativeness and export performance. But mediated effects did not indicate a significant risk tolerance. these results suggests the need to actively pursue innovation that more product differentiation capabilities required in export companies to small and medium-sized manufacturing. In other words, when attempting to highlight product differentiation, based on innovation, rather than risk tolerance. In terms of analyzing the mediated role of product differentiation capabilities, this study has theoretical implications for the future research to look at the antecedents of export performance from the perspective of dynamic capabilities and competitive advantage. Also, practical implications in this regard as the innovativeness and taking risks to all important to CEO of small manufacturing enterprises but, stranger in the foreign market competition environment, the role of innovation product is required on raising product differentiation capabilities are presented.

• Reproductive and Developmental Biology
• /
• v.34 no.3
• /
• pp.143-151
• /
• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.5
• /
• pp.2765-2769
• /
• 2013

Background: A close association between patterns identified by magnifying narrow-band imaging (M-NBI) and histological type has been described. M-NBI patterns were also recently reported to be related to the mucin phenotype; however, detials remain unclear. Materials and Methods: We investigated the cellular differentiation of gastric cancer lesions, along with their mucosal distribution observed by M-NBI. Ninety-seven depressed-type early gastric cancer lesions (74 differentiated and 23 undifferentiated adenocarcinomas) were visualized by M-NBI. Findings were divided into 4 patterns based on abnormal microvascular architecture: a chain loop pattern (CLP), a fine network pattern (FNP), a corkscrew pattern (CSP), and an unclassified pattern. Mucin phenotypes were judged as gastric (G-type), intestinal (I-type), mixed gastric and intestinal (M-type), and null (N-type) based on 4 markers (MAC5AC, MUC6, MUC2, and CD10). The relationship of each pattern of microvascular architecture with organoid differentiation indicated by cancer cell differentiation and its distribution in each histological type of early gastric cancer was investigated. Results: All CLP and FNP lesions were differentiated. The cancer cell distribution showed organoid differentiation in 84.2% (16/19) and 61.1% (22/36) of the two types of lesions, respectively, and there was a significant difference from the unclassified pattern with organoid differentiation (p<0.001). Almost all (94.7%; 18/19) CSP lesions were undifferentiated, and organoid differentiation was observed in 72.2% (13/18). There was a significant difference from the unclassified pattern with organoid differentiation (p<0.05). Conclusions: Cellular differentiation and distribution are associated with microvascular architecture observed by M-NBI.

• Archives of Craniofacial Surgery
• /
• v.19 no.3
• /
• pp.181-189
• /
• 2018

Background: Autogenous bone grafts have several limitations including donor-site problems and insufficient bone volume. To address these limitations, research on bone regeneration is being conducted actively. In this study, we investigate the effects of a three-dimensionally (3D) printed polycaprolactone (PCL)/tricalcium phosphate (TCP) scaffold on the osteogenic differentiation potential of adipose tissue-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMSCs). Methods: We investigated the extent of osteogenic differentiation on the first and tenth day and fourth week after cell culture. Cytotoxicity of the 3D printed $PCL/{\beta}-TCP$ scaffold was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, prior to osteogenic differentiation analysis. ADSCs and BMSCs were divided into three groups: C, only cultured cells; M, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold; D, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold with a bone differentiation medium. Alkaline phosphatase (ALP) activity assay, von Kossa staining, reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting were performed for comparative analysis. Results: ALP assay and von Kossa staining revealed that group M had higher levels of osteogenic differentiation compared to group C. RT-PCR showed that gene expression was higher in group M than in group C, indicating that, compared to group C, osteogenic differentiation was more extensive in group M. Expression levels of proteins involved in ossification were higher in group M, as per the Western blotting results. Conclusion: Osteogenic differentiation was increased in mesenchymal stromal cells (MSCs) cultured in the 3D printed PCL/TCP scaffold compared to the control group. Osteogenic differentiation activity of MSCs cultured in the 3D printed PCL/TCP scaffold was lower than that of cells cultured on the scaffold in bone differentiation medium. Collectively, these results indicate that the 3D printed PCL/TCP scaffold promoted osteogenic differentiation of MSCs and may be widely used for bone tissue engineering.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2004.07a
• /
• pp.24-24
• /
• 2004

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2003.10a
• /
• pp.86-86
• /
• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2006.10a
• /
• pp.78-78
• /
• 2006