• Title/Summary/Keyword: differential hosts

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New Definitive Hosts and Differential Body Indices of Isthmiophora hortensis (Digenea: Echinostomatidae)

  • Sohn, Woon-Mok;Na, Byoung-Kuk;Shin, Sung-Shik
    • Parasites, Hosts and Diseases
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    • v.55 no.3
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    • pp.287-294
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    • 2017
  • The present study was performed to record new definitive hosts of Isthmiophora hortensis, and to describe morphological characteristics derived from a variety of worm samples for clarification of its taxonomic validity. Morphological characteristics with dimensions were observed in worm samples (n=21) from naturally infected wild animals, including a raccoon dog Nyctereutes procyonoides from Gimhae-si (City), Gyeongsangnam-do, stray cats and a striped field mouse from several localities, and a wild boar Sus scrofa, from Gurye-gun (County), Jeollanam-do. In addition, adult flukes (n=45) recovered in albino rats experimentally infected with the metacercariae from a freshwater fish species were also subjected to morphological studies. The mean ratios of the body length (BL) to body width (BW) were 5.86 and 5.76 in worms from wild animals and experimental rats, respectively. Those of the ventral sucker to oral sucker were 2.92 and 3.01 in worms from 2 groups. The mean percentages of the hindbody length (HBL) to BL were 42.1 and 41.2 in 2 groups. Those of uterine fields to BL were 9.8 and 12.2 in the 2 worm groups. By the present study, the 2 species of wild animals, the raccoon dog and wild boar, have been added as new definitive hosts for I. hortensis. The morphological characteristics of adult flukes derived from a variety of host source were redescribed to support the taxonomic validity of this echinostome species.

Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot-Resistant Cultivars of Chinese Cabbage

  • Kim, Hun;Jo, Eun Ju;Choi, Yong Ho;Jang, Kyoung Soo;Choi, Gyung Ja
    • The Plant Pathology Journal
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    • v.32 no.5
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    • pp.423-430
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    • 2016
  • Clubroot disease caused by Plasmodiophora brassicae is one of the most serious diseases in Brassica crops worldwide. In this study, the pathotypes of 12 Korean P. brassicae field isolates were determined using various Chinese cabbage including 22 commercial cultivars from Korea, China, and Japan, and 15 inbred lines. All P. brassicae isolates exhibited the typical clubroot disease on non-clubroot resistant cultivar, indicating that the isolates were highly pathogenic. According to the reactions on the Williams' hosts, the 12 field isolates were initially classified into five races. However, when these isolates were inoculated onto clubroot-resistant (CR) cultivars of Chinese cabbage, several isolates led to different disease responses even though the isolates have been assigned to the same race by the Williams' host responses. Based on the pathogenicity results, the 12 field isolates were reclassified into four different groups: pathotype 1 (GN1, GN2, GS, JS, and HS), 2 (DJ and KS), 3 (HN1, PC, and YC), and 4 (HN2 and SS). In addition, the CR cultivars from Korea, China, and Japan exhibited distinguishable disease responses to the P. brassicae isolates, suggesting that the 22 cultivars used in this study, including the non-CR cultivars, are classified into four different host groups based on their disease resistance. Combining these findings, the four differential hosts of Chinese cabbage and four pathotype groups of P. brassicae might provide an efficient screening system for resistant cultivars and a new foundation of breeding strategies for CR Chinese cabbage.

Morphological Characteristics and Pathotype of Sphaceloma fawcettii Causing Citrus Scab in Korea. (우리나라 감귤 더뎅이병균의 형태적 특성과 병원형)

  • 송장훈;고영진
    • Korean Journal Plant Pathology
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    • v.14 no.4
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    • pp.303-307
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    • 1998
  • Citrus scab caused warty and scabby lesions on the surface of leaves, twigs and fruits of Satsuma mandarin. Warty lesions were mainly developed before July but scabby ones were developed during summer season in Cheju island, Korea. The casual organism of scab was morphologically identified as Sphaceloma fawcettii and was thought to be Tryon's pathotype of Elsinoe fawcettii, because it was pathogenic on rough lemon and Cleopatra mandarin but non-pathogenic on sour orange, grapefruit and sweet orange among the 5 differential hosts of E. fawcettii.

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Differential Cytokine and Immunoglobulin Expressions in the Small Intestine of Echinostoma hortense Infected BALB/c Mice

  • Jo, Yoon-Kyung;Lee, Dong-Sup;Kim, Sung-In;Lee, Ji-Sook;Oh, Ji-Eun;Sung, Ho-Joong
    • Biomedical Science Letters
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    • v.18 no.3
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    • pp.254-259
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    • 2012
  • Infections involving Echinostoma hortense (E. hortense) are considered to more severe than infections caused by other heterophyids. Although parasite expulsion by host immune responses attenuates the symptoms of infection, the detailed mechanisms of the host immune response need to be determined, especially in local immune responses involving cytokine and immunoglobulin expressions. We infected BALB/c mice with E. hortense and examined recovery rates together with expressions of multiple cytokines and immunoglobulins in the villi and crypts of the small intestine using immunohistochemistry. We observed a close correlation between worm expulsion rates and cytokine/immunoglobulin expressions in E. hortense infected mice. This study contributes to an understanding of the relationship between the immune response and parasite expulsion in hosts.

Comparative Analyses of Four Complete Genomes in Pseudomonas amygdali Revealed Differential Adaptation to Hostile Environments and Secretion Systems

  • Jung, Hyejung;Kim, Hong-Seop;Han, Gil;Park, Jungwook;Seo, Young-Su
    • The Plant Pathology Journal
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    • v.38 no.2
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    • pp.167-174
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    • 2022
  • Pseudomonas amygdali is a hemibiotrophic phytopathogen that causes disease in woody and herbaceous plants. Complete genomes of four P. amygdali pathovars were comparatively analyzed to decipher the impact of genomic diversity on host colonization. The pan-genome indicated that 3,928 core genes are conserved among pathovars, while 504-1,009 are unique to specific pathovars. The unique genome contained many mobile elements and exhibited a functional distribution different from the core genome. Genes involved in O-antigen biosynthesis and antimicrobial peptide resistance were significantly enriched for adaptation to hostile environments. While the type III secretion system was distributed in the core genome, unique genomes revealed a different organization of secretion systems as follows: type I in pv. tabaci, type II in pv. japonicus, type IV in pv. morsprunorum, and type VI in pv. lachrymans. These findings provide genetic insight into the dynamic interactions of the bacteria with plant hosts.

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions

  • Wang, Shuai;Cao, Aiping;Li, Xun;Zhao, Qunli;Liu, Yuan;Cong, Hua;He, Shenyi;Zhou, Huaiyu
    • Parasites, Hosts and Diseases
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    • v.53 no.3
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    • pp.253-258
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    • 2015
  • Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

Characterization of Sclerospora graminicola Isolates from Pearl Millet for Virulence and Genetic Diversity

  • Pushpavathi B.;Thakur R. P.;Rao K. Chandrashekara;Rao V. P.
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.28-35
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    • 2006
  • Virulence and genetic diversity were studied using 21 isolates of Sclerospora graminicola, the pearl millet downy mildew pathogen collected from major pearl millet growing areas of India. Variability for virulence was determined by inoculating a set of 10 differential hosts with the S. graminicola isolates in a greenhouse. The isolates varied for latent period (6.4 to 11 days), disease incidence (0 to $98\%$), virulence index (0 to 18.7) and oospore-production potential (1 to 4). Among the 21 isolates, Sg 139 (Rajasthan) was the most virulent and Sg 110 (Tamil Nadu) the least virulent. Based on virulence index (disease incidence$\time$slatent $period^{-1}$), the 21 isolates were classified into eight virulence groups. Genetic diversity among isolates was studied using AFLP markers. Based on similarity index of banding pattern, the 21 isolates were clustered into eight genotypic groups. The AFLP groupings, however, did not match with that of the virulence groupings, and these two were found independent. The isolate Sg 139 that remained distinct in both pathogenic and genetic groupings indicated its highly virulent nature. Implications of these results in downy mildew resistance breeding are discussed.

The Design for Traffic Container to use resources efficiently in DiffServ (DiffServ의 효율적인 자원활용을 위한 트래픽 컨테이너 설계)

  • Jang, Kyung-Sung;Kang, Dae-Wook
    • The KIPS Transactions:PartC
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    • v.11C no.1
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    • pp.115-122
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    • 2004
  • Diff-Serv is a mechanism by which network service providers can offer differing levels of network service to different traffic, in so providing quality of service (QoS) to their customers. Because this mechanism has been deployed just for fixed hosts with the Token Bucket mechanism, DiffServ have been suggested can not satisfy the mobility service or the differential serrlce for Individual traffics. In this paper, we suggest WFQ mechanism for traffic conditioner and scheduling method for monitoring the AggF(Aggregate Flow) which will be controlled in edge nodes and border routers. So it will control traffic rate dynamically and suggest efficient usability of bandwidth.

The Design of TC with WFQ for Effective Resource Sharing on Differentiated Service (Differentiated Service에서 공정한 자원 공유를 위한 WFQ 적용 TC 설계)

  • 장경성
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.41 no.2
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    • pp.31-40
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    • 2004
  • Diff-Serv(DS) is a mechanism by which network service providers can offer differing levels of network service to different traffic, in so providing quality of service(QoS) to their customers. Because this mechanism has been deployed just for fixed hosts with the Token Bucket mechanism according to AggF(Aggregate Flow) instead of each flow, DS can not suggest effective usability of traffic resources. In this paper, we use WFQ mechanism for traffic conditioner and scheduling method monitoring the AggF and the results will be used to control the next flows coming in TC. So it will control traffic rate dynamically and suggest efficient usability of bandwidth.

Pathotypes of Bacterial Spot Pathogen Infecting Capsicum Peppers in Korea

  • Wai, Khin Pa Pa;Siddique, Muhammad Irfan;Mo, Hwang-Sung;Yoo, Hee Ju;Byeon, Si-Eun;Jegal, Yoonhyuk;Mekuriaw, Alebel A.;Kim, Byung-Soo
    • The Plant Pathology Journal
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    • v.31 no.4
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    • pp.428-432
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    • 2015
  • Sixty-seven isolates of bacterial spot pathogen (Xanthomonas spp.) collected from six provinces of Korea were tested for the identification of their pathotypes and determination of their distribution throughout Korea in an effort to genetically manage the disease. Near isogenic lines of Early Calwonder (Capsicum annuum) pepper plants carrying $Bs_1$, $Bs_2$ and $Bs_3$, and PI235047 (C. pubescens) were used as differential hosts. Race P1 was found to be predominant, followed by race P7, and races P3 and P8 were also observed. This is the first report of races P7 and P8 in Korea. The races P7 and P8 were differentiated from the former races P1 and P3, respectively, on the basis of their ability to elicit hypersensitive reactions to PI235047.