• Title/Summary/Keyword: differential form

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Differential Expression of a Chimeric nos-npt II Gene in 9 Years Old Hybrid Poplars (Populus koreana x P. nigra)

  • Noh, Eun Woon;Lee, Jae Soon;Choi, Young Im;Lee, Hyo Shin;Bae, Eun Kyung;Lee, Ji Hee
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.15-19
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    • 2004
  • The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

Two cases of congenital atretic encephalocele misdiagnosed as dermoid cyst (유피낭종으로 오인된 atretic encephalocele 2례)

  • Kim, Jae-Hui;Cho, Jae-Min;Jung, Jin-Myung;Park, Eun-Sil;Seo, Ji-Hyun;Lim, Jae-Young;Park, Chan-Hoo;Woo, Hyang-Ok;Youn, Hee-Shang
    • Clinical and Experimental Pediatrics
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    • v.49 no.9
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    • pp.1000-1004
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    • 2006
  • Atretic cephalocele is a degenerative form of encephalocele, which is detected as a cystic mass in the head, primarily in infants. Its presentation and prognosis vary and depend on various factors, including the nature of the tissues within the cyst, other concomitant anomalies, the site of development, and the presence or absence of an embryonic straight sinus. We here report 2 cases of atretic encephalocele, that were transferred to our hospital because round tumors, misdiagnosed as dermoid cysts, were detected in their parietal lobes immediately after birth. On diagnostic and differential MRI, an embryonic straight sinus was detected while histochemical results indicated that the lesions contained cerebral tissues. Despite these structural anomalies, the two patients developed normally neurologically and no other anomalies were detected. We here discuss these two cases and present a review of the relevant literature.

Phosphoproteomic Analysis of AML14.3D10 Cell Line as a Model System of Eosinophilia

  • Ryu, Su-In;Kim, Won-Kon;Cho, Hyun-Ju;Lee, Phil-Young;Jung, Hye-Yun;Yoon, Tae-Sung;Moon, Jeong-Hee;Kang, Sung-Hyun;Poo, Ha-Ryoung;Bae, Kwang-Hee;Lee, Sang-Chul
    • BMB Reports
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    • v.40 no.5
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    • pp.765-772
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    • 2007
  • Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.

Comparison of Parallel Preconditioners for Solving Large Sparse Linear Systems on a Massively Parallel Machine (대형이산 행렬 시스템의 초대형병렬컴퓨터에서의 해법을 위한 병렬준비 행렬의 비교)

  • Ma, Sang-Baek
    • The Transactions of the Korea Information Processing Society
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    • v.2 no.4
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    • pp.535-542
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    • 1995
  • In this paper we present two preconditioners for solving large sparse linear systems arising from elliptic partial differential equations on massively parallel machines, such as the CM-5. Most massively parallel machines do heavily rely on the message-passing for the interprocessor communications. but according to the current manufacturing standards the cost of communications is very high compared to that of floating point arithmetic computations. Due to this we need an algorithm which minimizes the amount of interprocessor communication on the massively parallel machines. We will show that Block SOR(Successive Over Relaxation) method coupled with the multi-coloring technique is one of such preconditioner on the massively parallel machines, by conducting experiments in the CM-5. Also, we implemented the ADI(Alternation Direction Implicit) method in the CM-5, which has been conventionally one of the most powerful parallel preconditioner. Our experiment shows that Block SOR method coupled with the multi-coloring technique could yield a speedup with 50% efficiency with the range of number of processors form 16 to 512 for a matrix with dimension 512x512. On the other hand, the ADI method shows a very poor performance.

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Development of Hydrologic Simulation Model to Predict Flood Runoff in a Small Mountaineous Watershed (산지 소유역의 홍수유출 예측을 위한 모의발생 수문모형의 개발)

  • 권순국;고덕구
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.30 no.3
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    • pp.58-68
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    • 1988
  • Most of the Korean watersheds are mountaineous and consist of various soil types and land uses And seldom watersheds are found to have long term hydrologic records. The SNUA, a hydrologic watershed model was developed to meet the unique characteristics of Korean watershed and simulate the storm hydrographs from a small mountaineous watershed. Also the applicability of the model was tested by comparing the simulated storm hydrographs and the observed from Dochuk watershed, Gwangjugun, Kyunggido The conclusions obtained in this study could be summarized as follows ; 1. The model includes the simulation of interception, evaporation and infiltration for land surface hydrologic cycle on the single storm basis and the flow routing features for both overland and channel systems. 2. Net rainfall is estimated from the continuous computation of water balance at the surface of interception storage accounting for the rainfall intensities and the evaporation losses at each time step. 3. Excess rainfall is calculated by the abstraction of infiltration loss estimated by the Green and Ainpt Model from the net rainfall. 4. A momentum equation in the form of kinematic wave representation is solved by the finite differential method to obtain the runoff rate at the exit of the watershed. 5. The developed SNUA Model is a type of distributed and event model that considers the spatial distribution of the watershed parameters and simulates the hydrograph on a single storm basis. 6. The results of verification test show that the simulated peak flows agree with the observed in the occurence time but have relative enors in the range of 5.4-40.6% in various flow rates and also show that the simulated total runoff have 6.9-32% of relative errors against the observed. 7. To improve the applicability of the model, it was thought that more studies like the application test to the other watersheds of various types or the addition of the other hydrologk components describing subsurface storages are needed.

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A Comparative Study of the Nurse Licensure Exam Systems between the United States and South Korea (미국과 한국의 간호사 국가면허시험 문항개발과정 비교)

  • Kwak, Chanyeong;Yun, Soon-Nyoung;Kim, Shin-Jeong
    • Korean Journal of Adult Nursing
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    • v.25 no.6
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    • pp.622-632
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    • 2013
  • Purpose: This study was conducted to compare the nurse licensure examination system in Korea with that of the United States (US). Methods: Focus interviews with the administrative staff of the National Council of State Boards of Nursing (NCSBN) were held. two areas were explored in the interviews: the item development process and the general administrative operating procedures. Results: NCLEX-RN items are designed for entry-level practice. The items are subjected to a series of 12 steps including a item writing panel, a content and editorial review, a sensitivity panel, a pre-test, a Differential item functioning panel and a committee review. NCSBN is responsible for the test for registered nurses and one for practical nurses. In contrast the Korean National Health Personnel Licensing Board (KNHPLB) is responsible for nursing is and 21 other health related licensure. Another difference is that in Korea there is no consistent and specialized staff to develop question items. Items are developed by educators who are not active nurses (this last statemtne is not a finding but a point of view usually not given in findings.). Conclusion: Korean nurses form the largest group of health related job categories and the most direct to health care consumers. Therefore, the nursing licensure exam should be acknowledged as the most influential licensure exam in health care services. We recommend a nursing specialized licensing institution to be established with active nurse' participation in item development process to reflect clinical practice into licensure exam.

Study on the cooling water supply method to the cooling water injection nozzle in the steam generator (증기발생기 내 냉각수 분사 노즐로의 냉각수 공급 방법에 관한 연구)

  • Oh, Jeong-hwa;Shin, Min-kyu;Cho, Young-seok;Ko, Young-sung
    • Journal of Aerospace System Engineering
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    • v.14 no.5
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    • pp.66-72
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    • 2020
  • In this study, a method for increasing the initial water supply was employed to protect the water injection nozzle by the flame when supplying the water to the steam generator. During the initial steam generator test, the flow rate was controlled by using the only venturi, but cooling water was not supplied to the combustion chamber at the beginning of combustion, thereby resulting in damage to the water nozzle. To solve this problem, a venturi and an orifice were configured in parallel to increase the initial supply flow rate to form a differential pressure between the water manifold and the combustion chamber. Venturi and orifice supply sequences were established through the water flow tests, and combustion tests were conducted for final verification. Consequently, a continuous supply of the cooling water at the beginning of combustion was achieved, and the experiment was successfully performed without damaging the cooling water nozzle.

Phenolic Composition, Fermentation Profile, Protozoa Population and Methane Production from Sheanut (Butryospermum Parkii) Byproducts In vitro

  • Bhatta, Raghavendra;Mani, Saravanan;Baruah, Luna;Sampath, K.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.10
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    • pp.1389-1394
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    • 2012
  • Sheanut cake (SNC), expeller (SNE) and solvent extractions (SNSE) samples were evaluated to determine their suitability in animal feeding. The CP content was highest in SNSE (16.2%) followed by SNE (14.7%) and SNC (11.6%). However, metabolizable energy (ME, MJ/kg) was maximum in SNC (8.2) followed by SNE (7.9) and SNSE (7.0). The tannin phenol content was about 7.0 per cent and mostly in the form of hydrolyzable tannin (HT), whereas condensed tannin (CT) was less than one per cent. The in vitro gas production profiles indicated similar y max (maximum potential of gas production) among the 3 by-products. However, the rate of degradation (k) was maximum in SNC followed by SNE and SNSE. The $t^{1/2}$ (time taken for reaching half asymptote) was lowest in SNC (14.4 h) followed by SNE (18.7 h) and SNSE (21.9 h). The increment in the in vitro gas volume (ml/200 mg DM) with PEG (polyethylene glycol)-6000 (as a tannin binder) addition was 12.0 in SNC, 9.6 in SNE and 11.0 in SNSE, respectively. The highest ratio of $CH_4$ (ml) reduction per ml of the total gas, an indicator of the potential of tannin, was recorded in SNE (0.482) followed by SNC (0.301) and SNSE (0.261). There was significant (p<0.05) reduction in entodinia population and total protozoa population. Differential protozoa counts revealed that Entodinia populations increased to a greater extent than Holotricha when PEG was added. This is the first report on the antimethanogenic property of sheanut byproducts. It could be concluded that all the three forms of SN byproducts are medium source of protein and energy for ruminants. There is a great potential for SN by-products to be incorporated in ruminant feeding not only as a source of energy and protein, but also to protect the protein from rumen degradation and suppress enteric methanogenesis.

One-Pot Reaction Involving Two Different Amines and Formaldehyde Leading to the Formation of Poly(Macrocyclic) Cu(II) Complexes

  • Lee, Yun-Taek;Kang, Shin-Geol
    • Bulletin of the Korean Chemical Society
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    • v.33 no.8
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    • pp.2517-2522
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    • 2012
  • New polynuclear poly(hexaaza macrocyclic) copper(II) complexes $[1](ClO_4)_{2n}{\cdot}(H_2O)_{2n}$, $[2](ClO_4)_{2n}{\cdot}(H_2O)_{2n}$, and $[3](ClO_4)_{2n}{\cdot}(H_2O)_{2n}$ have been prepared by the one-pot reaction of formaldehyde with ethylenediamine and 1,2-bis(2-aminoethoxy)ethane, 1,3-diaminopropane, or 1,6-diaminohexane in the presence of the metal ion. The polymer complexes contain fully saturated 14-membered hexaaza macrocyclic units (1,3,6,8,10,13-hexaazacyclotetradecane) that are linked by $N-(CH_2)_2-O-(CH_2)_2-O-(CH_2)_2-N$, $N-(CH_2)_3-N$, or $N-(CH_2)_6-N$ chains. The mononuclear complex $[Cu(H_2L^5)](ClO_4)_4$ ($H_2L^5$ = a protonated form of $L^5$) bearing two $N-(CH_2)_2-O-(CH_2)_2-O-(CH_2)_2-NH_2$ pendant arms has also been prepared by the metal-directed reaction of ethylenediamine, 1,2-bis(2-aminoethoxy)ethane, and formaldehyde. The polymer complexes were characterized employing elemental analyses, FT-IR and electronic absorption spectra, molar conductance, X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and scanning electron micrograph (SEM). Electronic absorption spectra of the complexes show that each macrocyclic unit of them has square-planar coordination geometry with a 5-6-5-6 chelate ring sequence. The polymer complexes as well as $[Cu(H_2L^5)]^{4+}$ are quite stable even in concentrated $HClO_4$ solutions. Synthesis and characterization of the polynuclear and mononuclear copper(II) complexes are reported.

Investigation of Stereo-dynamic Properties for the Reaction H+HLi by Quasi-classical Trajectory Approach

  • Wang, Yuliang;Zhang, Jinchun;Jiang, Yanlan;Wang, Kun;Zhou, Mingyu;Liang, Xiaorui
    • Bulletin of the Korean Chemical Society
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    • v.33 no.9
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    • pp.2873-2877
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    • 2012
  • Quasi-classical trajectory (QCT) calculations of H+HLi reaction have been carried out on a new potential energy surface of the ground state reported by Prudente et al. [Chem. Phys. Lett. 2009, 474, 18]. The four polarization-dependent differential cross sections have been carried out in the center of mass (CM) frame at various collision energies. The reaction probability for the depletion channel has been studied over a wide collision energy range. It has been found that the collision energy decreases remarkably reaction probability, which shows the expected behavior of the title reaction belonging to an exothermic barrierless reaction. The results are in good agreement with previous RMP results. The P(${\theta}_r$), P(${\phi}_r$) and P(${\theta}_r,\;{\phi}_r$) distributions, the k-k'-j' correlation and the angular distribution of product rotational vectors are presented in the form of polar plots. The average rotational alignment factor <$P_2(j{\prime}{\cdot}k)$> as a function of collision energy is also calculated. The results indicate that the collision energy has a great influence on the polarization of the product rotational angular momentum vector j'.