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Effect of Partial Laser Assisted Hatching on Mouse Embryos (레이져를 이용한 부분적 보조부화술이 생쥐 수정란의 부화에 미치는 효과)

  • Kim, Dong-Hoon;Kim, Myo-Kynng;Lee, Hoi-Chang;Ko, Duck-Sung;Park, Won-Il;Kwon, Hynck-Chan;Lee, Ho-Joon
    • Clinical and Experimental Reproductive Medicine
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    • v.28 no.2
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    • pp.147-153
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    • 2001
  • Objective: The present study was performed to investigate the efficiency of partial laser assisted hatching (p-LAH; lased 1/2 ZP width from ZP edge) on hatching of mouse blastocysts. Methods: We used non-contact $1.48{\mu}m$ diode laser (MTM, Switzland) to create a precise hole on zona pellucida. 2-cell embryos were collected from the mouse (ICR) oviduct at 48 hours after hCG administration. Collected 2-cell embryos were cultured in the P-1 medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after $20{\sim}22$ hours in culture. After conventional (c-LAH) or partial laser assisted hatching, the embryos were further cultured in P-1 medium supplemented with 0.4% BSA for 3 days. To compare efficiency of complete and partial laser assisted hatching, hatching rate, hatching time and blastocyst diameter and zona pellucida thickness at hatching time were investigated. Embryos were examined every 12 hours. Blastocyst diameter and zona pellucida thickness at hatching time were measured with an ocular micrometer. Results: Hatching rates of p-LAH group (84.2%) was significantly higher than that of control group (39.3%), but there was no difference between the p-LAH (84.2%) and c-LAH (91.2%). p-LAH group was hatched 12 hours earlier than control group, but hatched 12 hours later than c-LAH group. The diameter of blastocyst at hatching time of p-LAH group ($113.1{\pm}6.4{\mu}m$) was smaller than that of control group ($122.2{\pm}5.0{\mu}m$), but larger than that of c-LAH group ($102.2{\pm}2.7{\mu}m$). Zona pellucida thickness at hatching time of p-LAH group ($6.4{\pm}0.9{\mu}m$) was thicker than that of control group ($4.5{\pm}1.5{\mu}m$), but thinner than that of c-LAH group ($10.0{\pm}0.8{\mu}m$). Conclusion: These results suggest that p-LAH may maintains the cell arrangement of early embryos to ensure successful development and prevent precocious hatching of blastocyst when compare to c-LAH and conventional (acidic tyrode) AH. Thus, p-LAH may provide a valuable and effective AH technique for human ART program.

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Evaluation of incremental sheet forming characteristics for 3D-structured aluminum sheet - part 2 (3D 구조 알루미늄 판재의 점진판재성형 특성 평가 (제2보))

  • Kim, Young-Suk;Do, Van-Cuong;Ahn, Dae-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.3
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    • pp.1585-1593
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    • 2015
  • 3D-structured (embossed) aluminum sheets have been used in the heat insulation purpose for automative exhaust parts because of increasing their surface areas and stiffness reinforcement imposed in making the embossing pattern. However, there are many restrictions in press forming of the embossed sheet compared with the flat sheet (non-embossed one) because of its difference in the mechanical properties and the geometrical 3-dimensional shape. In this paper we investigated the deformation characteristic of embossed aluminum sheet in the incremental sheet forming process which has frequently used in the design verification and the trial manufacturing of sheet products. The single point incremental forming (SPIF) experiments for the rectangular cone forming using the CNC machine with a chemical wood-machined die and a circular tool shape showed that the formability of the embossed sheet are better than that of the flat sheet in view of the maximum angle of cone forming. This comes from the fact that the embossed sheet between the tool and the elastic die wall is plastically compressed and the flatted area contributes to increase the plastic deformation. Also the tool path along the outward movement from the center showed a better formability than that of the inward movement from the edge. However the surface quality for the tool path along the outward movement evaluated from the surface deflection is inferior than that of the tool path along the inward movement.

Stereo Matching Using Distance Trasnform and 1D Array Kernel (거리변환과 1차원 배열을 이용한 적응적 스테레오 정합)

  • Chang, Yong-Jun;Ho, Yo-Sung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.4
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    • pp.387-394
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    • 2016
  • A stereo matching method is one of the ways to obtain a depth value from two dimensional images. This method estimates the depth value of target images using stereo images which have two different viewpoints. In the result of stereo matching, the depth value is represented by a disparity value. The disparity means a distance difference between a current pixel in one side of stereo images and its corresponding point in the other side of stereo images. The stereo matching in a homogeneous region is always difficult to find corresponding points because there are no textures in that region. In this paper, we propose a novel matching equation using the distance transform to estimate accurate disparity values in the homogeneous region. The distance transform calculates pixel distances from the edge region. For this reason, pixels in the homogeneous region have specific values when we apply this transform to pixels in that region. Therefore, the stereo matching method using the distance transform improves the matching accuracy in the homogeneous regions. In addition, we also propose an adaptive matching cost computation using a kernel of one dimensional array depending on the characteristic of regions in the image. In order to aggregate the matching cost, we apply a cross-scale cost aggregation method to our proposed method. As a result, the proposed method has a lower average error rate than that of the conventional method in all regions.

WELD REPAIR OF GAS TURBINE HOT END COMPONENTS

  • Chaturvedi, M.C.;Yu, X.H.;Richards, N.L.
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.235-243
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    • 2002
  • Ni-base superalloys are used extensively in industry, both in aeroengines and land based turbines. About 60% by weight of most modern gas turbine engine structural components are made of Ni-base superalloys. To satisfy practical demands, the efficiency of gas turbine engines has been steadily and systematically increased by design modifications to handle higher turbine inlet or firing temperatures. However, the increase in operating temperatures has lead to a decrease in the life of components and increase in costs of replacement. Moreover, around 80% of the large frame size industrial/utility gas turbines operating in the world today were installed in the mid-sixties to early seventies and are now 25 to 30 years old. Consequently, there are greater opportunities now to repair and refurbish the older models. Basically, there are two major factors influencing the weldability of the cast alloys: strain-age cracking and liquation cracking. Susceptibility to strain-age cracking is due to the total Ti plus AI content of the alloy; Liquation cracking is due either to the presence of low melting constituents or constitutional liquation of constituents. Though Rene 41 superalloy has 4.5wt.% total Ti and Al content and falls just below the safe limit proposed by Prager et al., controlled grain size and special heat treatments are needed to obtain crack-free welds. Varying heat treatments and filler materials were used in a laboratory study, then the actual welding of service parts was carried out to verity the possibility of crack-tree weld of components fabricated from Rene 41 superalloy. The microstructural observations indicated that there were two kinds of carbides in the FCC matrix. MC carbides were located along the grain boundaries, while M$_{23}$C$_{6}$ carbide was located both inter and intra granularly. Two kinds of filler materials, Rene 41 and Hastelloy X were used to gas tungsten arc weld a patch into the sheet metal, along with varying pre-weld heat treatments. The microstructure, hardness and tensile tests were determined. The service distressed parts were categorized into three classes: with large cracks, with medium cracks and with small or no visible cracks. No significant difference in microstructure among the specimens was observed. Specimens were cut from the corner and the straight edge of the patch repair, away from the corner. The only cracks present were found to be associated with inadequate surface preparation to remove oxidation. Guidelines for oxide removal and the welding procedures developed in the research enabled crack-free welds to be produced.d.

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PHOTOELASTIC STRESS ANALYSIS ON THE MANDIBLE CAUSED BY IMPLANT OVERDENTURE (임플랜트 Overdenture의 Bar설계에 따른 하악지지조직의 광탄성학적 응력분석)

  • Kang Jeong-Min;Vang Mong-Sook
    • The Journal of Korean Academy of Prosthodontics
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    • v.32 no.2
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    • pp.327-353
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    • 1994
  • This study was performed to evaluate the effects of number and alignment of implant fixture and various bar designs on the retention of denture and the stress distribution. Six kinds of photoelastic mandibular models and nine kinds of overdenture specimens were designed. A unilateral vertical load was gradually applied on the right first molar to calculate the maximal dislodgement load of each specimen. A unilateral vertical load of 17 Kgf was applied on the right first molar and a vertical load of 10 Kgf was applied on the interincisal edge region. The stress pattern which developed in each photoelastic model was analyzed by the reflection polariscope. The results obtained were as follows: 1. The maximal dislodgement load reversely increased with the distance from the loading point to the implant fixture, while it linearly increased with that from the most posterior implant fixture to the mesial clip. The maximal dislodgement load also increased with the use of a cantilever bar. 2. Under the posterior vertical load, the stress to the supporting tissue of the denture base increased with the distance from the loading point to the implant future. The stress concentration on the apical area of the implant future reversely increased with the distance from the loading point to the implant future. 3. In the overdentures supported by two implant fixtures under the posterior vertical load. the specimen implanted on lateral incisor areas with a cantilever bar exhibited more favorable stress distribution than that without a cantilever bar. The specimen implanted on the canine areas without a cantilever bar, however, exhibited more favorable stress distribution. 4. In the overdentures supported by three implant fixtures. the specimen implanted ell the midline and canine areas exhibited more favorable stress distribution than that implanted oil the midline and the first premolar areas. 5. In the overdentures supported by four implant fixtures. the specimen implanted with two adjacent implant fixtures exhibited more favorable stress distribution than that implanted at equal distance under the posterior vertical load. 6. Under the anterior vertical load, the overdentures supported by three implant fixtures exhibited stress concentration on the supporting structure of the middle implant future. In overdentures supported by two or four implant futures, no significant difference was noted in stress distribution between the types of bars. These results indicate that the greater the number of implant fixtures, the better the stress distribution is. A favorable stress distribution may be obtained in the overdentures supported by two or three implant fixtures, if the location and the design of the bar are appropriate.

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Frontiers in Magneto-optics of Magnetophotonic Crystals

  • Inoue, M.;Fedyanin, A.A.;Baryshev, A.V.;Khanikaev, A.B.;Uchida, H.;Granovsky, A.B.
    • Journal of Magnetics
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    • v.11 no.4
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    • pp.195-207
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    • 2006
  • The recently published and new results on design and fabrication of magnetophotonic crystals of different dimensionality are surveyed. Coupling of polarized light to 3D photonic crystals based on synthetic opals was studied in the case of low dielectric contrast. Transmissivity of opals was demonstrated to strongly depend on the propagation direction of light and its polarization. It was shown that in a vicinity of the frequency of a single Bragg resonance in a 3D photonic crystal the incident linearly polarized light excites inside the crystal the TE- and TM-eigen modes which passing through the crystal is influenced by Brags diffraction of electromagnetic field from different (hkl) sets of crystallographic planes. We also measured the faraday effect of opals immersed in a magneto-optically active liquid. It was shown that the behavior of the faraday rotation spectrum of the system of the opal sample and magneto-optically active liquid directly interrelates with transmittance anisotropy of the opal sample. The photonic band structure, transmittance and Faraday rotation of the light in three-dimensional magnetophotonic crystals of simple cubic and face centered cubic lattices formed from magneto-optically active spheres where studied by the layer Korringa-Kohn-Rostoker method. We found that a photonic band structure is most significantly altered by the magneto-optical activity of spheres for the high-symmetry directions where the degeneracies between TE and TM polarized modes for the corresponding non-magnetic photonic crystals exist. The significant enhancement of the Faraday rotation appears for these directions in the proximity of the band edges, because of the slowing down of the light. New approaches for one-dimensional magnetophotonic crystals fabrication optimized for the magneto-optical Faraday effect enhancement are proposed and realized. One-dimensional magnetophotonic crystals utilizing the second and the third photonic band gaps optimized for the Faraday effect enhancement have been successfully fabricated. Additionally, magnetophotonic crystals consist of a stack of ferrimagnetic Bi-substituted yttrium-iron garnet layers alternated with dielectric silicon oxide layers of the same optical thickness. High refractive index difference provides the strong spatial localization of the electromagnetic field with the wavelength corresponding to the long-wavelength edge of the photonic band gap.

Analysis of PLAN Modernization Trend and Prospects for Balance of U.S-China Naval power in the East Asia (중국해군(PLAN)의 현대화 추세와 동아시아 지역의 미·중 해군력 균형 전망)

  • Kwon, Jeong Wook
    • Strategy21
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    • s.43
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    • pp.5-28
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    • 2018
  • The tensions between the U.S and China, which form the two pillars of the G2 era, seem to have persisted even after the Trump administration inaugurated. The strong confrontation between the two in recent foreign security issues may drive to develop an inadvertent military conflict, and it is high likely to occur in the maritime are. The purpose of this study is not only to analyze the balance of modernized naval forces in the PLAN through naval strategy changes and weapons system modernization trend, but also to predict the impact of the geographical proximity difference on the balance of naval forces in the disputed areas. It examined the impact of distance and geography on naval power by assessing the modernization pattern of the PLAN and capabilities in the context of two scenarios at different distances from China by 2020: one centered on Taiwan and the other on the Spratly Islands. The PLAN's strategy had impact on operational concept and forces construction. First, from the viewpoint of operation operational concept, it can be seen that the passive defense is changing into active defense. Second, in terms of power construction, it can threaten the surface and submarines of U.S power from a distance. And they generated follow three features; The ocean is not the focus of Chinese submarines, Horizontal and vertical expansion of Chinese naval vessels, The improvement of the suppression ability as the Chinese naval modernization ratio increases. The strength of the PLAN is dominant over the U.S in terms of reserves, and it can complement the qualitative deterioration by utilizing nearby bases in the vicinity of the mainland, such as the Taiwan Strait. However, due to the shortage of aircraft carriers, there is a possibility that it will take some time to secure the advantage of air and ocean in the amphibious operation. Therefore, as the dispute is prolonged, China may fail to achieve its original goal. In addition, the lack of cutting edge Commanding Ships may bring to weaken the C2 capabilities. At results, it is expected that PLAN will not be able to have a superiority in the short term due to lagging behind U.S advanced technology. Nevertheless, PLAN has strengthened its naval power through modernization sufficiently and it is highly likely to use force. Especially, it is more likely in the region where the naval power operation like the Taiwan Strait is possible with the almost equality to that of the United States. China will continue to use its naval forces to achieve a rapid and decisive victory over U.S in the close area from the land.

A Study on the Management Situation and Development Plan in Private Bakery for Korean: Focused on the Bakery's Owner (국내 자영 제과점의 경영 실태 분석 및 발전 방안 연구)

  • Lee, Kwang Suck;Hwang, Yoon Kyung;Lee, Hee Tae;Ahn, Hye Lyung;Jung, Hyo Sun
    • Culinary science and hospitality research
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    • v.21 no.2
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    • pp.215-229
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    • 2015
  • The purpose of this study was to identify the management situation and development plan targeting private bakery owners who are doing business in major cities. For research, the questionnaire survey was conducted targeting 69 owners who are operating bakery at the point of data collection, and the data were analyzed using frequency analysis and paired-sample t-test using SPSS. The results of the study were as follows. Owners' baking career and management career were mostly more than 10 years. They are currently managing bakeries with at least 4 competing bakeries and point out franchise-type bakeries as major competitors. Also, they responded that the most important factor for strengthening competitive edge of shop was employees and that the enhancement in technology and employees with adequate techniques are important for improving sales. Also, the result of paired sample t-test revealed that the communication had the biggest difference between importance and satisfaction perceptions among taste, sanitation, service, price, product, location, and communication. Limitations and future research directions are also discussed.

Clinical convergence angle and rounding radius on tooth preparation for zirconia all-ceramic crown (지르코니아 전부도재관 지대치 삭제시 축면 경사각과 만곡 반경에 대한 조사)

  • Kim, Hye-Eun;Woo, Yi-Hyung;Pae, Ah-Ran;Kim, Hyeong-Seob
    • The Journal of Korean Academy of Prosthodontics
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    • v.49 no.1
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    • pp.22-28
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    • 2011
  • Purpose: This article attempted to examine how teeth for restoration is made in a clinical practice and utilize it as future educational material of teeth formation and basic data for additional research. Materials and methods: This experiment investigated the models sent to milling center for production of zirconia crowns. After scanned with Lava CAD/CAM System (3M ESPE, Seefeld, Germany), they are measured on 'ImageJ (version 1.32j, National Institutes of Health, USA)' program and compared and analyzed. Convergence angle from mesio-distal surfaces and bucco-lingual surfaces of each teeth are measured. Also, bucco-lingual diameter of the region lowered as much as 0.4 mm from incisal edge in anterior teeth except canines.(This measure is defined as the Peak 0.4) The analysis of data between each group was conducted by Windows SPSS statistic program, and was proved significant on 95% confidence level by independent t-test, one-way ANOVA and multiple analysis (Sheff${\'{e}}$ test). Results: The mean value of convergence angle was $18.67^{\circ}$ It is ranked as molar ($26.70^{\circ}$) > premolar ($16.87^{\circ}$) > anterior teeth ($14.81^{\circ}$) in the order of mesio-distal convergence angle; anterior teeth ($22.32^{\circ}$) > molar ($20.93^{\circ}$) > premolar ($15.41^{\circ}$) in the order of bucco-lingual convergence angle. The mean value of Peak 0.4 was 1.18 mm. Conclusion: Convergence angle of abutment of zirconia all ceramic crown has difference depending on the location in the arch. Due to the nature of production of zirconia all ceramic crown, convergence angle of abutment and line angle finishing degree can have an effect on internal suitability of restoration.

Analysis for the RCS of a Trihedral Corner Reflector with Consideration of the Effect of Front Surface (지표면 영향을 고려한 삼각 전파 반사기의 RCS 분석)

  • Shin, Jong-Chul;Kweon, Soon-Koo;Oh, Yi-Sok;Kim, Se-Young;Jeon, Byeong-Tae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.6
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    • pp.723-730
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    • 2012
  • The radar cross section(RCS) of a trihedral corner reflector(TCR) should be accurately computed when it is used as an external calibration target for a satellite synthetic aperture radar(SAR) calibration campaign. This paper presents the RCS analysis on a trihedral corner reflector which is installed on a calibration site, using the wave reflection from the rough surface and the wave diffraction from the TCR edges. The results in this paper show quantitatively the effect of the front surface on the RCS of a TCR. The difference of the RCS between a TCR in air and a TCR on a ground surface is computed by including the interaction term which consists of the edge diffraction from the TCR edges and the surface reflection from the front rough surface. The reflection coefficient of a randomly rough surface is a function of the surface roughness and dielectric constant of the surface. The RCS of $10{\lambda}$ size TCR on a ground is 0.46 dB higher than TCR in air at 9.65 GHz, and this can reach at maximum 1.55 dB depending on a surface condition and TCR size. The effect of the front surface on the RCS of a TCR increases, as the surface roughness decreases, the soil moisture increases, and the size of TCR in wavelength decreases.