• 대기
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• 제24권3호
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• pp.343-364
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• 2014

This study presents local and synoptic conditions associated with extreme heavy snowfall events in the Yeongdong region, as well as the temporal and spatial variability of these conditions. During the last 12 years (2001~2012), 3 extreme snowfall events occurred in the Yeongdong region, which recorded daily snowfall greater than 50 cm, respectively. In these events, one of the noticeable features is the occurrence of heavy hourly snowfall greater than 10 cm. It was reported from satellite analysis that these heavy snowfall may be closely related to mesoscale convective clouds. In this paper the 3 extreme events are examined on their synoptic environments associated with the developments of mesoscale convective system using numerical model output. These 3 events all occurred in strongly forced synoptic environments where 500 and 300 hPa troughs and 500 hPa thermal troughs were evident. From the analysis of diagnostic variables, it was found in all 3 events that absolute vorticity and cold air advection were dominant in the Yeongdong region and its surrounding sea at upper levels, especially at around 500 hPa (absolute vorticity: $20{\sim}60{\times}10^{-5}s^{-1}$, cold air advection: $-10{\sim}-20^{\circ}C$ $12hr^{-1}$). Moreover, the spatial distributions of cold advection showed mostly the shape of a narrow band along the eastern coast of Korea. These features of absolute vorticity and cold advection at 500 hPa were sustained for about 10 hours before the occurrence of maximum hourly snowfall.

• Smart Structures and Systems
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• 제14권5호
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• pp.811-830
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• 2014

A numerical study has been carried out to simulate an innovative monitoring procedure to detect and localize damage in reinforced concrete beams retrofitted with carbon fiber reinforced polymer (CFRP) unidirectional laminates. The main novelty of the present simulation is its ability to conduct the electromechanical admittance monitoring technique by considerably compressing the amount of data required for damage detection and localization. A FEM simulation of electromechanical admittance-based sensing technique was employed by applying lead zirconate titanate (PZT) transducers to acquire impedance spectrum signatures. Response surface methodology (RSM) is finally adopted as a tool for solving inverse problems to estimate the location and size of damaged areas from the relationship between damage and electromechanical admittance changes computed at PZT transducer surfaces. This statistical metamodel technique allows polynomial models to be produced without requiring complicated modeling or numerous data sets after the generation of damage, leading to considerably lower cost of creating diagnostic database. Finally, a numerical example is carried out regarding a steel-reinforced concrete (RC) beam model monotonically loaded up to its failure which is also retrofitted by a CFRP laminate to verify the validity of the present metamodeling monitoring technique. The load-carrying capacity of concrete is predicted in the present paper by utilizing an Ottosen-type failure surface in order to better take into account the passive confinement behavior of retrofitted concrete material under the application of FRP laminate.

• 여성건강간호학회지
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• 제24권2호
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• pp.105-115
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• 2018

Purpose: To investigate the subjective health and health-related quality of life (HRQoL) in Haenyo. Methods: Subjects were 100 elderly Haenyo in Jeju island who belonged to a fishing-village society. Main variables were activities of daily living (ADL), instrumental ADL (IADL), the HRQoL, subjective health, and depression. Subjective health and differences of HRQoL by variables were analyzed by t-test or ANOVA using IBM SPSS Statistics 23. Hierarchical multiple regression was executed to examine the effects of the major factors on the quality of life. Results: The mean age was 69.9 years, the mean period for diving career was 51.5 years, and work hours per month were 37.8. Comorbidity of diseases was 2.74, and the common health problems were osteoporosis and headache/dizziness. HRQoL was significantly different by age (F=4.52, p=.013), education (F=6.10, p=.003), muljil work years (F=3.93, p=.050), depression (t=-3.04, p=.030), subjective health state (F=30.62, p<.01), and degenerative arthritis (F=-2.38, p=.019). In the final model by hierarchical multiple regression, ADL/IADL (${\beta}=.41$, p<.001), depression (${\beta}=-.29$, p<.001), and subjective health (${\beta}=.43{\sim}.51$, p<.001) were significant and explained 63.5% of the total variance of HRQoL. Conclusion: Haenyo have specific health problems different from those of elderly women in general. ADL/IADL, depression and subjective health affected their HRQoL. It is clear that Haenyos' health problems need further study to improve their health.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.854-861
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• 2014

The diagnosis of Brucella abortus is mainly based on serological methods using antibody against LPS, which has diagnostic problems. Therefore, to solve this problem, we evaluated two proteins of B. abortus, Cu/Zn superoxide dismutase (SodC) and outer membrane proteins 2b porin (Omp2b). The genes were cloned and expressed in a pMAL system, and the recombinant proteins, rOmp2b and rSodC, were purified as fusion forms with maltose-binding protein. The identity of the proteins was confirmed by SDS-PAGE and Western blot analysis with sera of mice infected with B. abortus. Production of cytokines and nitric oxide (NO) was investigated in RAW 264.7 cells and mouse splenocytes after stimulation with the proteins. Moreover, cellular and humoral immune responses were investigated in BALB/c mice after immunization with the proteins. TNF-${\alpha}$, IL-6, and NO were significantly inducible in RAW 264.7 cells. Splenocytes of naive mice produced IFN-${\gamma}$ and IL-4 significantly by stimulation. Moreover, number of IgG, IFN-${\gamma}$, and IL-4 producing cells were increased in immunized mice with the two proteins. Production of IgG and IgM with rOmp2b was higher than those with rSodC in immunized mice. These results suggest that the two recombinant proteins of B. abortus may be potential LPS-free proteins for diagnosis.

• 한국항만경제학회지
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• 제29권4호
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• pp.175-199
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• 2013

본 연구는 부산신항 컨테이너터미널 운영사가 수립하여 실행한 터미널운영전략의 효과를 전략실행프로세스(XPP)모델에 의거하여 진단하고 전략실행력을 제고할 수 있는 방안을 제시하고자 하였다. 연구결과에 의하면, 부산항 컨테이너터미널 운영사의 전략실행프로세스 요인 모두 미흡한 수준으로 전략의 수립부터, 전략의 구체화, 조직과 정렬 운영계획과의 연계 그리고 전략의 모니터링과 업그레이드 전체과정을 합리적이고 유기적으로 실시간 실행할 수 있는 맞춤형 XPP시스템 구축이 매우 시급한 것으로 사료된다. 또한 북항은 부산신항에 비하여 컨테이너 전용터미널로서 경쟁력을 점차 상실되고 있어 북항재개발을 서둘러 북항의 용도변경을 하여야만 부산항 전체의 경쟁력을 확보할 수 있는 것으로 판단된다. 경영자의 리더쉽요인은 중요한 요인이지만 미흡한 수준으로서 CEO의 리더쉽을 발휘할 수 있는 여건 마련과 역량 강화가 시급하다. 터미널 경쟁력에 중요한 인적자원 역량 강화를 위해서는 전략수립을 구체화하는 과정으로 BSC Map의 체계적인 작성과 실행 가능한 전략적 과제의 도출 및 이들의 전체 조직에의 전파와 구체적인 실행계획 및 예산이 뒷받침 되어야 하며, 이를 위한 전략관리 기획본부나 팀같은 조직구축이 시급한 것으로 사료된다.

• 정보화연구
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• 제11권1호
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• pp.99-111
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• 2014

현재 개발도상국 공적개발원조(ODA) 사업에서 통신망 구축, PC 보급 등 정보화 기반 확충을 도와주고 있다. 국가간 정보격차 해소를 위한 대한민국 IT 봉사단 파견사업도 해당국의 정보화 활용수준을 높이는데 큰 기여를 하고 있다. 본 연구는 개발도상국 정보화 수준평가 항목을 도출하고 도출된 항목들의 중요도를 분석하였다. 또한 정보화 수준 평가 항목들 간에 어떠한 상관관계가 있는지 공분산구조분석을 통해 검증하였다. 본 연구 결과는 다음과 같은 시사점을 얻을 수 있다. 개발도상국의 정보화 기반 수준이 높으면 정보화 활용 수준에 영향을 미치는 것은 자명하다. 하지만 개발도상국의 정보화 전략 수준과 환경 수준을 높여주는 것이 당장의 정보화 기반 수준을 높이는 것 보다 장기적으로 정보화 활용 수준을 높이는데 더 큰 영향력을 미친다. 좀 더 장기적인 개발도상국 정보격차 해소는 정보화 전략 수준과 환경 수준을 높여줄 때 가능한 것으로 보인다.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.529-535
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• 2011

Single-chain variable fragment (scFv) is a fusion protein of the variable regions of the heavy (VH) and light (VL) chains of immunoglobulin, connected with a short linker peptide of 10 to about 20 amino acids. In this study, the scFv of a monoclonal antibody against the third domain of human CD4 was cloned from OKT4 hybridoma cells using the phage display technique and produced in E. coli. The expression, production, and purification of anti-CD4 scFv were tested using SDS-PAGE and Western blot, and the specificity of anti-CD4 scFv was examined using ELISA. A 31 kDa recombinant anti-CD4 scFv was expressed and produced in bacteria, which was confirmed by SDS-PAGE and Western blot assays. Sequence analysis proved the ScFv structure of the construct. It was able to bind to CD4 in quality ELISA assay. The canonical structure of anti-CD4 scFv antibody was obtained using the SWISS_MODEL bioinformatics tool for comparing with the scFv general structure. To the best of our knowledge, this is the first report for generating scFv against human CD4 antigen. Engineered anti-CD4 scFv could be used in immunological studies, including fluorochrome conjugation, bispecific antibody production, bifunctional protein synthesis, and other genetic engineering manipulations. Since the binding site of our product is domain 3 (D3) of the CD4 molecule and different from the CD4 immunological main domain, including D1 and D2, further studies are needed to evaluate the anti-CD4 scFv potential for diagnostic and therapeutic applications.

• Journal of Korean Neurosurgical Society
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• 제61권5호
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• pp.548-558
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• 2018

Objective : Diagnosing acute cerebral infarction is crucial in determining prognosis of stroke patients. Although many serologic tests for prompt diagnosis are available, the clinical application of serologic tests is currently limited. We investigated whether $S100{\beta}$, matrix metalloproteinase-9 (MMP-9), D-dimer, and heat shock protein 70 (HSP70) can be used as biomarkers for acute cerebral infarction. Methods : Focal cerebral ischemia was induced using the modified intraluminal filament technique. Mice were randomly assigned to 30-minute occlusion (n=10), 60-minute occlusion (n=10), or sham (n=5) groups. Four hours later, neurological deficits were evaluated and blood samples were obtained. Infarction volumes were calculated and plasma $S100{\beta}$, MMP-9, D-dimer, and HSP70 levels were measured using enzyme-linked immunosorbent assay. Results : The average infarction volume was $12.32{\pm}2.31mm^3$ and $46.9{\pm}7.43mm^3$ in the 30- and 60-minute groups, respectively. The mean neurological score in the two ischemic groups was $1.6{\pm}0.55$ and $3.2{\pm}0.70$, respectively. $S100{\beta}$, MMP-9, and HSP70 expressions significantly increased after 4 hours of ischemia (p=0.001). Furthermore, $S100{\beta}$ and MMP-9 expressions correlated with infarction volumes (p<0.001) and neurological deficits (p<0.001). There was no significant difference in D-dimer expression between groups (p=0.843). The area under the receiver operating characteristic curve (AUC) showed high sensitivity and specificity for MMP-9, HSP70 (AUC=1), and $S100{\beta}$ (AUC=0.98). Conclusion : $S100{\beta}$, MMP-9, and HSP70 can complement current diagnostic tools to assess cerebral infarction, suggesting their use as potential biomarkers for acute cerebral infarction.

• 한국동물위생학회지
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• 제31권3호
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• pp.331-338
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• 2008

The study was carried out to evaluate the diagnostic efficacy of the tube agglutination test (TAT), enzyme-linked immunosorbent assay (ELISA) and the 2-Mercaptoethanol agglutination test (2-MAT) to detect human brucellosis patients in Korea. We examined 87 serum samples of people in the rural farm areas where bovine brucellosis had been reported. People in this study were divided into seven groups- farmers and their families, veterinarians, veterinary quarantine workers, livestock health control officers, artificial inseminators, livestock traders and healthy control individuals. Among 87 people, 65 were males and 22 were females ranging in age from 13 to 72 years. Of 87 serum samples, ELISA detected 21.84%, TAT detected 11.50% and 2-MAT detected 8.05% Brucella positive sera. Brucella specific IgG ELISA antibody titer was recorder higher in the individuals between the ages of 50 and 65 years. The highest prevalence rate of brucellosis(29.4%) was recorded in the cattle farmers and their family members followed by quarantine veterinary office workers (25%) and practicing veterinarians 01.1%). The majority of the Brucella sero-positive individuals in this study had a history of direct contact with animals.

• 한국진공학회지
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• 제15권4호
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• pp.338-346
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• 2006

반도체 공정에 사용되는 진공펌프는 가혹한 운전조건과 비선형적 특성으로 인하여 고장시점을 정확히 예측해내기가 어려운데 이로 인해 불량품이 양산되거나 불필요한 재원이 낭비되는 등의 문제가 발생하게 된다. 따라서 펌프의 운전상태를 올바르게 모니터링하고 고장 지점을 정확히 인지해 적절한 펌프 교체 시점을 알려주는 진공펌프 상태진단 모델의 개발은 매우 시급하고도 중대한 문제라 할 수 있겠다. 본 연구에서는 다변량 통계기법을 이용하여 영향력 있는 인자들을 종합적으로 고려하였으며 최종적으로 Hotelling's T2 통계량을 이용한 진공펌프 상태진단 모델을 제안하였다. 핵심적인 알고리즘으로는 Multiway Principal Component Analysis(MPCA)와 Dynamic Time Warping Algorithm(DTW Algorithm) 기법 등이 사용되었다.