• 한국콘텐츠학회논문지
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• 제17권10호
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• pp.307-321
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• 2017

본 연구는 중 고등학교 학생 2,260명을 대상으로 Big 5 성격요인에 따른 청소년 성격특성에 발달적 변화가 있는지 알아보고자 한다. 성별과 학년에 따라 성격 5요인의 발달적 변화에 차이를 보이는지 확인하였고, 더불어 신경증 하위요인을 추가적으로 분석함으로써 청소년기 문제행동 시기와 특성을 확인 하였다. 분석에는 일원배치변량분석(One-way ANOVA)을 사용하였으며 유의미한 차이가 난 경우 사후검증을 실시하였다. 연구 결과를 요약하면 다음과 같다. 첫째, 청소년의 성격 5요인 특성이 성별에 따라 차이가 있는 것으로 나타났다. 개방성, 성실성, 외향성은 여자가 남자보다 높게 나타났고, 신경증은 남자가 여자보다 높게 나타났다. 둘째, 청소년의 성격 5요인 특성 모두에서 학년에 따라 차이가 있는 것으로 나타났다. 셋째, 청소년기 성격특성에 대한 성별에 따른 학년별 발달적 경향성에서도 성별 간 다른 양상을 보이는 것으로 나타났다. 특히 성별과 학년에 따른 분석에서 남자는 중등 2학년, 여자는 고등 3학년에서 성격특성이 두드러질 것으로 나타났다. 그리고 신경증과 관련된 외현화 행동문제는 중등 1 2학년에서 내현화 행동문제는 고등 3학년에서 주로 발현될 것으로 나타났다. 따라서 본 연구에서는 현재 우리나라 청소년 성격특성의 발달적 변화를 확인할 수 있었으며, 또한 성별과 학년에 따라 상이한 정신건강문제가 발현 될 수 있는 것으로 나타났다.

• Reproductive and Developmental Biology
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• 제28권1호
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• pp.13-20
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• 2004

This study was conducted to investigate the developmental ability of caprine embryos after somatic cell interspecies nuclear transfer. Donor cells were obtained from an ear-skin biopsy of a caprine, digested with 0.25% trypsin-EDTA in PBS, and primary fibroblast cultures were established in TCM-199 with 10% FBS. After maturation, expanded cumulus cells were removed by vigorous pipetting in the presence of 0.3% hyaluronidase. The matured oocytes were dipped in D-PBS plus 10% FBS＋7.5 $\mu\textrm{g}$/ml cytochalasin B and 0.05 M sucrose. The reconstructed oocytes were electrically fused with donor cells in 0.3 M mannitol fusion medium. After the electofusion, embryos were activated by electric stimulation. Interspecies nuclear transfer embryos with bovine cytoplasts were cultured in TCM-199 medium supplemented with 10% FBS including bovine oviduct epithelial cells for 7∼9 day. On the other hand, the NT embryos with porcine cytoplasts were cultured in NCSU-23 medium supplemented with 10% FBS for 6∼8 day at $39^{\circ}C, 5% CO_2$ in air. In caprine-bovine NT embryos, the cleavage(2-cell) rate was 36.8% in confluence and 43.8% in serum starvation. The developmental rate of morula- and blastocyst-stage embryos was 0.0% in confluence and 18.8% in serum starvation. In caprine-porcine NT embryos, the cleavage(2-cell) rate was 76.7% in confluence and 66.7% in serum starvation. The developmental rate of morula and blastocyst stage embryos was 3.3% in confluence and 3.0% in serum starvation, and no significant difference was observed in synchronization treatment between donor cells. In caprine-bovine NT embryos, the cleavage(2-cell) rate of cultured donor cells was 30.8% and 17.6% in 5∼9 and 10∼14 passage(P＜0.05). The developmental rate of morula and blastocyst stage embryos were significantly higher(P＜0.05) in 5∼9 passage(23.1%) than in 10∼14 passage(0.0%) of cultured donor cells. In caprine-porcine NT embryos, the cleavage rate was significantly higher(P＜0.05) in 5∼9 passage(86.7%) than in 10∼14 passage(50.0%) of cultured donor cells. The developmental rate of morula and blastocyst stage embryos were 3.3 and 0.0% in 5∼9 and 10∼14와 passage of cultured donor cells. In caprine-bovine NT embryos, the developmental rate of morula and blastocyst stage embryos were 22.6% in interspecies nuclear transfer, 33.9% in in vitro fertilization and 28.1% in parthenotes, which was no significant differed. The developmental rate of morula and blastocyst stage embryos with caprine-porcine NT embryos were lower(P＜0.05) in interspecies nuclear transfer(5.1%) than in vitro fertiltzation(26.9%) and parthenotes(37.4%).

• 한국수정란이식학회지
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• 제11권3호
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• pp.201-210
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• 1996

This experiment was carried out to evaluate the effect of early mouse embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells (BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated cultured in D-PBS /15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation in the embryos were examined under the fllowing treatments; 1) TCM 199 added 15% HCS, 2) Ham's F-10 added 15% HCS, 3) MediCult IVF medium, 4) TCM 199 added 15% HCS + BOEC, 5) TCM 199 added 15% HCS + POEC, 6) Ham's F40 added 15% HCS + BOEC, 7) Ham's F-10 added 15% HCS + POEC,8) MediCult IVF medium + BOEC, 9) MediCult IVF medium + POEC. For a comparative study of in vitro development for 96 hours after hCG injection, were cultured with oviductal epithelial cell and media only. The obtained results were 2-cell embryos developed to the blastocyst stage in TCM 199, Ham's F-10 and MediCult IVF medium at the rates of 84.4,83.2 and 81.6%. respectively. The higher developmental rates(91~97%) of blastocyst formation was appeared when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham's F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal epithelial cells but significant difference in co-culture system in comparison between media only system and co-cultures. In conclusions, oviductal epithelial cells, BOEC and POEC, when co-culture with mouse early embryos improved the rates of development, blastocyst and hatching. Therefore, it is suggested that co-culture system using oviductal epithelial cells improve early embryonic developtnent in mouse.

• 한국수정란이식학회지
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• 제28권2호
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• pp.149-155
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• 2013

Mullerian inhibiting substance (MIS) is a member of the TGF-${\beta}$ (transforming growth factor-${\beta}$) family whose members play key roles in development, suppression of tumour growth, and feedback control of the pituitary-gonadal hormone axis. MIS is expressed in a highly tissue-specific manner in which it is restricted to male Sertoli cells and female granulose cells. The serum levels of MIS in prenatal and postnatal ICR mice were measured using the enzyme-linked immuno-solvent assay (ELISA) using the MIS/AMH antibody. Mice were grouped by age: the significant periods were at the onset of development. During sex organ differentiation, no remarkable difference between female and male foetus MIS serum levels (both<0.1 ng/ml) was observed. However, MIS serum levels in pregnant mice markedly changed (4.5~12.2 ng/ml). After birth, postnatal female and male mice serum MIS levels changed considerably (male: <0.1~138.5 ng/ml, female: 5.3~103.4 ng/ml), and the changing phase were diametrically opposed (male: decreasing, female: fluctuating). These findings suggest that MIS may have strong associations with not only develop-ment but also puberty. For further studies, establishing the standard MIS serum levels is of importance. Our study provides the basic information for the study of MIS interactions with reproductive organ disability, cancer, and the effect of other hormone or menopause. We hypothesise that if MIS is regularly injected into middle-age women, meno-pause will be delayed. We detected that serum MIS concentration curves change with age. The changing phase is different between males and females, and this difference is significant after birth. Moreover, MIS mRNA is expressed during the developmental period (prenatal) and also in the postnatal period. This finding indicates that MIS may play a significant role in the developmental stage and in growth after birth.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.64-64
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• 2001

To facilitate the widespread application of somatic cell cloning, improvements in blastocyst production efficiency and subsequent fetal viability are required. Area where technical improvements are needed include donor cell treatments, starvation and passage numbers. This study was carried out to investigate the effect of serum-starvation and passage on the development of ear skin fibroblast cells cloned embryos. A skin biopsy was obtained from the ear of a 2-year-old Korean Hanwoo female. The cells were cultured in 10% FBS＋DMEM up to 2-3 months(up to 10 passages) and then used. In Experiment 1, the Korean bovine Ear Skin Fibroblast cells (KbESF) were either serum starved (culture in 0.05% FBS＋DMEM) or serum fed (10% FBS＋DMEM) for 4-7 days Prior to NT In Experiment 2, the KbESF cells used for nuclear transfer in these experiments were from passages 2 to 10. The development of 208 nuclear transfer (NT) embryos reconstructed from either serum starved or serum fed ear skin fibroblast was assessed. NT embryos reconstructed from serum starved and serum fed cells showed the same developmental rate (cleavage 80.16 vs. 85.37%; blastocyst 20.63 vs. 19,51%). The development of 590 nuclear transfer (NT) embryos reconstructed from passage 2 to 10 was assessed. We observed the same developmental rates for embryos derived from later Passages as compared with those embryos from early passages(blastocyst from 16.69 to 27.91%, average 20.17%). There was no significant difference between serum-fed and serum-starved donor cells. We observed no difference in developmental rates for embryos derived from 2 to 10 passages. These data show that prolonged culture and serum starvation does not affects the cloning competence of adult somatic cells.

• 한국과학교육학회지
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• 제37권4호
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• pp.659-668
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• 2017

이 연구는 진화 개념의 발달 단계가 전공과 국가에 따라서 유의미한 차이가 있는지 확인하기 위하여 진행되었다. 이 연구를 위하여 한국과 미국의 대학생의 자료를 생물 전공과 비생물 전공에 따라 구분하여 수집하였다. Ha(2007)이 개발한 도구를 사용하여 Ha(2016)의 분석방법에 근거하여 순위선다형으로 분석하였다. 연구 결과 진화적 사고의 과학사적 발달 과정(창조, 목적, 의도, 용불용설, 자연선택)을 토대로 순위선다방식으로 분석하는 방법이 한국과 미국, 전공과 비전공 학생에게도 적합하게 적용될 수 있는 방법임이 확인되었다. 진화 개념의 발달 단계를 확인한 결과 우리나라 대학생들은 용불용설 수준, 미국의 대학생들은 목적론적 설명 수준에 있는 것으로 확인되었다. 전공과 국가에 따른 진화 개념의 발달 수준은 진화 설명소재와 교호작용효과가 있는지 확인한 결과 유의미한 교호작용효과가 없는 것으로 확인되었다. 진화 개념의 발달 단계와 종교성은 유의미한 관련성이 있었다. 이 연구 결과들을 토대로 진화 개념의 발달 특성에 대해서 논의한다.

• Child Health Nursing Research
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• 제17권1호
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• pp.39-47
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• 2011

Purpose: This study was done to determine whether a parenting education program using responsive teaching strategies is effective for parenting stress, maternal interactional behavior and behavioral problems in children at risk for Attention Deficit Hyperactivity Disorder (ADHD). Methods: Participants in this study were 17 elementary school children and their parents. The ADHD risk group was determined by scores on the Korean-ADHD Rating Scale. The parenting education program was developed based on Dr. Mahoney's responsive teaching curriculum. Mothers participated in the program once a week for 8 sessions. Treatment outcome was evaluated using the Korean version of the Child Behavior CheckList (K-CBCL), parenting stress, maternal interactional behavior, and child pivotal developmental behavior. Data were analyzed using Wilcoxon signed rank test, Mann-Whitney test, and regression analysis. Results: After the parenting education program, no significant difference in parenting stress (Z=-1.00, p=.320) was found, but there was a significant decrease in the child's internal behavior problems (Z=-2.05, p=.040), and also a significant improvement in maternal interactional behavior and a significant difference in child pivotal developmental behavior (Z=-2.67, p=.008). Conclusion: The results indicate that parenting education programs based on responsive teaching strategies are effective and that application of a program is recommended to prevent behavioral problems and improve maternal child interaction for children at risk for ADHD.

• 복식문화연구
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• 제5권3호
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• pp.159-175
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• 1997

The purpose of the study is to analyze the pattern of human developmental growth with empirical anthropometric data for the establishment of the sizing system of junior high school student\`s uniforms. The sample size was 881 boys and 762 girls between age 12 and 14. An anthropometric database used for this study was the 1992 national anthropometric survey of Korea. The result obtained are as follows ; 1. Several items of anthropometric data on junior high school students students showed significant differences in accordance with age and sex. In the meantime, there was no difference on the item of height under the age of 12 between sex, however, significant difference on that age of 13 or over. The hip girth was the biggest growth rate among tyhe item of girth. The somatotype of boys is straight, while girls\` shows a curved line. The bodytype of girls shows slim waist, fat belly and hip girth. 2. The result of factor analysis indicated that the first factor was composed with girth, depth and width-measures, and the second factor of height, length-measures. The third factors were consisted of items of representing bodytype of trunk, and the fourth, bodytype of lower part of trunk. 3. The result of cluster analysis indicated that boys were sybdivided into 3 types and girls 4 types.

• The Journal of Korean Physical Therapy
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• 제15권3호
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• pp.251-264
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• 2003

Alcohol exposure during development leads to significant long-term neurobehavior dysfunction and central nervous system alteration. The purpose of this study was to determine the effects of enriched enviroment in developmental period through motor behavior test and expression of BDNF. Neonatal rat exposed to alcohol on postnatal days 4 through 10 were studied. Female Sprague-Dawley pups were assigned to two groups. Experimental group(EG) via 4.5 g kg-1day-1 of ethanol was housed in enriched enviornment for 9 weeks. The main result of this study were as follows: 1. There was significant difference in the mean of weight change between control and experimental group. 2. In motor behavior test, there was significant difference in the mean of weight change between control and experimental group. 3. Regarding the immunoreactivity of BDNF were higher appeared experimental group than control group. In conclusion, the present results reveals that enriched enviroment in developmental period is to be extremely useful in neuronal reprganization and motor behavior improvement after alcohol exposure.

• Clinical and Experimental Reproductive Medicine
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• 제18권2호
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• pp.173-179
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• 1991

The purpose of this study was to evaluate the in vitro developmental promoting effect of human amniotic fluid (AF) on pronucleate 1-cell mouse embryos. The AF was obtained from five patients undergoing amniocentesis for the routine diagnosis of fetal abnormality. The supernatant was filtered ($0.22{\mu}m$) after centrifugation and stored at $-20^{\circ}C$. One-cell embryos were cultured in four study groups (10% AF, 0.4% BSA, 10% AF+0.4% BSA & no-supplement in Ham's F10) for 6 days (EXP. 1). Significantly more embryos hatched in 10% AF (P<0.0l), although no difference was found among other three groups. The embryos were also cultured in varous concentration of AF (0, 10, 50 & 100%) for 7 days (EXP. 2). The rate of hatched blastocysts was significantly higher in 10%- and 50% group than in 0% and 100%- one at day 6 (P<0.05) and day 7 (P<0.005), although no difference was found between 10% and 50%- group.