• Molecules and Cells
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• v.42 no.10
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• pp.711-720
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• 2019

Sink strength optimizes sucrose import, which is fundamental to support developing seed grains and increase crop yields, including those of rice (Oryza sativa). In this regard, little is known about the function of vacuolar invertase (VIN) in controlling sink strength and thereby seed size. Here, in rice we analyzed mutants of two VINs, OsVIN1 and OsVIN2, to examine their role during seed development. In a phenotypic analysis of the T-DNA insertion mutants, only the OsVIN2 mutant osvin2-1 exhibited reduced seed size and grain weight. Scanning electron microscopy analysis revealed that the small seed grains of osvin2-1 can be attributed to a reduction in spikelet size. A significant decrease in VIN activity and hexose level in the osvin2-1 spikelets interfered with spikelet growth. In addition, significant reduction in starch and increase in sucrose, which are characteristic features of reduced turnover and flux of sucrose due to impaired sink strength, were evident in the pre-storage stage of osvin2-1 developing grains. In situ hybridization analysis found that expression of OsVIN2 was predominant in the endocarp of developing grains. A genetically complemented line with a native genomic clone of OsVIN2 rescued reduced VIN activity and seed size. Two additional mutants, osvin2-2 and osvin2-3 generated by the CRISPR/Cas9 method, exhibited phenotypes similar to those of osvin2-1 in spikelet and seed size, VIN activity, and sugar metabolites. These results clearly demonstrate an important role of OsVIN2 as sink strength modulator that is critical for the maintenance of sucrose flux into developing seed grains.

• KIEAE Journal
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• v.14 no.1
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• pp.59-66
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• 2014

G-SEED aims for establishing green environmental building with energy saving and has started for evaluating with multi-residential complex in 2002 by the Ministry of Land, Infrastructure & Transport (MOLIT) and the Ministry of Environment to develop the current 10 building types and new & existing building types to be applied. Since G-SEED has been developed for more than 10 years, the strategy of globalization is needed with constant review itself and comparison between other assessments to understand current situation from the global perspectives. This study draws similarities and differences of G-SEED and LEED criteria by the comparison from re-constructed LEED criteria based on G-SEED. "Energy & Pollution" is the most concerned category in both assessments as the result of analysis on category rates. Next concerns are "Material & Resources", "Indoor Environment" and "Water Management". The unique categories in both criteria are "Ecological Environment" in G-SEED and "Innovation & Design Process" in LEED. The conclusion of this research is that two assessments are organized at different locations and with different strategies for developing green building but there are common concerns such as energy saving, material use, indoor environment. As a result, it is expected to use fundamental data for developing G-SEED for global standard.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.310-317
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• 2006

This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

• Journal of the Korean Solar Energy Society
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• v.35 no.6
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• pp.35-42
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• 2015

The proper use of the passive and active design elements are important for the realization of green buildings. The G-SEED (Green Standard for Energy and Environmental Design) has been implemented in Korea to promote green building construction reflecting these design elements. In this paper, evaluation items of G-SEED which related to the passive design were selected. And improvement direction for the G-SEED was proposed through the analysis of the application status of the passive design elements for the G-SEED certified buildings. This results can be used for developing study on the future improvement of the assessment criteria of G-SEED.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.329-333
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• 2006

Soybean quality is determined based on protein content, lipid content and fatty acid composition, and several functional components including isoflavones, anthocyanins and functional activity. Because the level of each component changes during seed development, it is necessary to know the concentration of quality-related components in developing seeds. Little is known of the pattern of changes in quality-related components. Seeds from field-grown soybean was harvest from the $R_6$ stage to the $R_8$ stage in 2004. Seed characteristics and the level of nutritional components were examined. Seed moisture content was dropped rapidly after the $R_7$ stage in the tested varieties. Seed growth rate was the highest from the beginning of the $R_6$ stage to the mid-$R_6$ stage. Chlorophyll content was decreased rapidly in pods and seeds. However, seed growth period from the $R_6\;to\;R_8$ was 35 days. The crude protein content was. increased dramatically between 63 DAF and 70 DAF and then increased slightly. The pattern of isoflavone accumulation was nearly similar to that of seed weight increase. From the late $R_6$ stage to the $R_7$, the accumulation rate was higher as compared to other stages. The angiotensin inhibitory activity was increased according to seed development from 63 ($R_6$) to 84 DAF ($R_8$). The difference of inhibitory activity in heated soybean powder, however, was not great among stages. The inhibitory activity was affected by heating treatment. The most effective heating time was 10 min. Excessive heating longer than 30 min resulted in a lowered inhibitory activity of soybean on ACE.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.11-15
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• 2006

Five seed samples of carrot were tested to detect Alternaria spp. by blotter method. A. alternata and A. radicina were detected from all the seed samples as high as $25.8-70.5\%$ and $37.5-63.5\%$, respectively. A. dauci was detected from four seed samples as low as $0.5-7.5\%$. The three Alternaria spp. were detected from the pericarp and the seed coat and endosperm of the carrot seeds but not from the embryo by component plating test. A. alternata and A. radicina were much more detected from the pericarp than the seed coat and endosperm. A. dauci was detected from the pericarp and the seed coat and endosperm at similar rate. The seed sample which was most severely infected with A. radicina showed the lowest rate of germination in the test on top of paper (TP). In the TP test, differences in total infection rate of A. radicina and A. dauci of the seed samples were very closely correlated with those in incidence of seedling rot on the seed samples. However, there was no correlation between infection rate of A. alternata and rate of germination or seedling rot of the seed samples. Soil test for seedling growth revealed that there was no correlation between differences in total infection rate of A. radicina and A. dauci and those in rate of normal seedlings of the seed samples.

• Applied Microscopy
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• v.25 no.1
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• pp.15-29
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• 1995

Legumin was purified from the endosperm cells of the ginseng seed and analyzed its characteristics. Distributional patterns of the legumin in the endosperm cells were identified using the immunocytochemical method. Legumin was glycoprotein composed of two subunits, molecular weights about 33,000 and 25,000 respectively. The molecular shape of purified legumin stained negatively seems to have hexagonal structure about 10 nm in size. It was localized at the rER, dictyosomes, and in the vacuoles at the early developing stage. Legumin was glycosylated in the dictyosomes and transported from the dictyosomes to the vacuoles. Legumin was accumulated into the central vacuole via the dictyosomes while the endosperm cells were developing. The armorphous proteins containing legumin were scattered randomly within the central vacuoles, which were aggregated together and became gradually spherical shape. Legumin was distributed within the globular protein bodies in the endosperm cells of matured seed. However legumin was not found in the globoids located in the protein bodies.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.133-138
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• 2003

Five seed kernel namely, Neem (Azadirachta indica A. Juss.), Pongamia (Pongamia glabra L. Pierre), Tamarind (Tamarindus indica L.), Mahua (Madhuca indica Gmel.) and Shikakai (Acacia cancinna De.) were tested against hatching of eggs and larval mortality of Meloidogyne incognita causing root knot disease in mulberry along with Furadan (Carbofuran) and Bionema (a bioformulation developed from Verticillium chlamydosporium) for comparison. Results revealed that highest hush-up of hatching was observed in Neem (77.40%) and Pongamia (75.99%) seed kernel extracts at 100% concentration over the check. Similarly, highest larval mortality was observed in Neem and Pongamia by 76.00% and 74.50%, respectively at 100% concentration after 72 hrs of exposure period. Pot culture studies revealed that pre-application of seed kernel powders (20 days before inoculation of nematode) found to be more effective in controlling the root knot disease than post application. In pre application of seed kernel powders, maximum reduction of root knots was observed in case of Neem seed kernel powder (54.85%) followed by Pongamia (51.9%). Similar trend was also observed in reduction of egg masses/plant and nematode population ／250 cc soil. Rest of the seed kernel extracts was found to be less effective in suppression of hatching, enhancing the larval mortality and controlling the root knot disease. However, application of Furadan and Bionema tested for the comparison were found to be more effective than seed kernel powders. The generated information seems to be useful in developing an ecofriendly integrated approach for the control of root knot nematode disease in mulberry.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.242-252
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• 2012

Seed storage oils are essential resources for not only human and animal diets but also industrial applications. The primary goal of this study was to increase seed oil content through comparative analysis of two seed-specific promoters, AtFAE1 from Arabidopsis Fatty Acid Elongase 1 gene and BnNapin from Brassica napus seed storage protein gene. AtWRI1 and AtDGAT1 genes encoding an AP2-type transcription factor and a Diacylglycerol Acyltransferase 1 enzyme, respectively, were expressed under the control of AtFAE1 and BnNapin promoters in Arabidopsis. The total seed oil content in all transgenic plants was increased by 8-11% compared with wild-type seeds. The increased level of oil content in AtWRI1 and AtDGAT1 transgenic lines under the control of both promoters was similar, although the activity of the BnNapin promoter is much stronger than that of AtFAE1 promoter in the mature stage of developing seeds where storage oil biosynthesis occurs at a maximum rate. This result demonstrates that the AtFAE1 promoter as well as the BnNapin promoter can be used to increase the seed oil content in transgenic plants.

• Architectural research
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• v.20 no.4
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• pp.121-128
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• 2018

Given the barriers to implement green building rating systems, Building Information Modeling (BIM) was suggested as an effective solution integrating information into one model and saving substantial time to facilitate certification process. Synergies between BIM and Leadership in Energy and Environment Design (LEED), the most widely used rating system, have been researched for a few decades. This paper demonstrates literature review about the development of integration between BIM and LEED. The research focuses on synergies between BIM and Green Standard for Energy & Environmental Design (G-SEED) in Korea, as one of important strategies to mitigate greenhouse gas emission. The research compares LEED and G-SEED related items based on evaluation contents. The result manifests G-SEED and LEED share many common items in different degrees. Therefore, it is entirely possible for G-SEED and BIM to adapt same developing mode of LEED and BIM. Moreover, the study measures the potential of BIM in application process of G-SEED certification by investigation of credits in LEED and G-SEED can be earned by BIM. The results of paper indicate the documentation support LEED and G-SEED may be prepared directly, semi-directly and indirectly via sustainability analyses software in BIM.