• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.6
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• pp.611-616
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• 2001

The bacteriological and physiochemical analysis of seawater in Tongyeong harbor was conducted to evaluate sanitary conditions, The samples were collected at 6 stations established once a month from January to December, 2000. During the study period, the ranges of temperature, transparency, chemical oxygen demand, dissolved oxygen, dissolved nitrogen, phosphate and chlorophyll-a were $6.8\sim25.2^{\circ}C,\;1.0\sim2.5\;m,\;1.79\sim2.41\;mg/L,\;5.7\sim10.1\;mg/L,\;6.59\sim10.53{\mu}g-at/L,\;0.56\sim1.01{\mu}g-at/L\;and\;1.21\sim9.54\;mg/m^3$, respectively, The viable cell counts of seawater in Tongyeong harbor ranged from $3.0\times10^4CFU/mL\;to\;6.9\times10^6CFU/mL$. The coliform group and fecal coliform MPN's of the samples were ranged $23\~4,600\;MPN/100\;mL$ (means 540 MPN/100 mL) and $11\~1,600\;MPN/100\;mL$ (means 210 MPN/100 mL), respectively, The coliform group was classified with IMViC reactions and pathogenic vibrios were analyzed. Two hundred eighteen strains that were obtained from seawater samples in Tongyeong harbor represented Escherichia coli group, $66.1\%$; Citrobacter freundii group, $11.0\%$; Enterobacter aerogenes, $9.6\%$; and unknown, $13.3\%$, respectively. During the study period, infectious bacteria such as Vibrio cholerae O1, Salmonella sp. and Shigella sp. were not detected from the samples, but detection ratios of V. parahaemolyticus, V cholerae non-O1 and V. vulnificus were $10.0\sim30.1\%$.

• Korean Journal of Food Science and Technology
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• v.27 no.4
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• pp.449-457
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• 1995

Aseptic packaged cooked rice without oxygen absorber and EVOH material was not suitable for good quality during long storage at room temperature. Quality change of aseptic packaged cooked rice with OPP/EVOH/EPL(P-1), PET/EVOH/EPL(P-2) and OPP/EVOH/LLDPE(P-3) for lid material and FX and TSQ for oxygen absorber was observed during storage(60 days) at $20^{\circ}C,\;30^{\circ}C\;and\;40^{\circ}C$. To evaluate quality change of aseptic packaged cooked rice, water content, color, pH, acidity, hardness, and total plate count were measured periodically. The results showed that moisture content was decreased as storage time increased while storage temperature showed no significant effect. P-2 and FX combination had the lowest evaporation of water. L value was decreased as storage time increased while a value increased in all treatment. P-2 and FX combination had the best result for color value. Titratable acidity and pH of P-3 increased up to 20, 5.5, respectively at the end of storage while the other treatment showed no significant effect. Hardness of aseptic packaging of cooked rice was decreased as temperature of storage increased while lid materials and oxygen absorber types showed no significant effect. Result of total plate count showed that there was no detection for $20^{\circ}C\;and\;30^{\circ}C$ storage temp. while more than $1{\times}10^6\;CFU/g\;for\;40^{\circ}C$ storage temp. at the end of storage. In conclusion, aseptic packaged cooked rice could be stored and distributed for 6 month at room temp. and effect of lid materials on quality change of aseptic packaged cooked rice was more significant than oxygen absorber.

• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.33-39
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• 2001

To develop a method for separation process using Sep-pak $C_18$, simultaneous and systematic analysis of 8 permitted and 11 non-permitted synthetic food colors in Korea, optimization of analysis conditions for reverse phase ion-pair high performance liquid chromatography was carried out. For the best result of Sep-pak $C_18$ separation the pH of color standard mixture solution was $5{\sim}6$ and 0.1% HCl-methanol solution were set as eluent. The colors eluated from Sep-pak $C_18$ cartridge were determined and confirmed by high performance liquid chromatography with a photodiode array detector at 420 nm for yellow colors type, at 520 nm for red colors type, at 600 nm for blue and green colors type and at 254 nm for mixed colors. Conditions for HPLC analysis were as follows: column, Symmetry $C_18$ (5 m, 3.9 mm $i.d.{\times}150\;mm$); mobile phase, 0.025 M ammonium acetate (containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (65 : 25 : 10) and 0.025 M ammonium acetate(containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (40 : 50 : 10); flow rate, 1 mL/min. It takes 35 minutes for simultaneaus analysis and 18 minutes for systematic analysis. The detection limits range of each colors were $0.01{\sim}0.05\;{\mu}g/g$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.401-408
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• 2011

Type 2 diabetes mellitus (NIDDM) is a metabolic disorder that is characterized by high blood glucose in the context of insulin resistance and relative insulin deficiency. In order to control the type 2 diabetes mellitus, anti-hyperglycemic effect of Triticum aestivum L. water extracts (TAWE) was investigated in 7 week old male diabetic C57BL6/J-ob/ob mice. For the experiments, the diabetic animal model ob/ob mice and non-diabetic animal model lean mice were divided into 3 groups: non-treatment control group (Control), and two experimental groups orally treated with 25 or 100 mg/kg/day dose of TAWE (TAWE-25 and TAWE-100, respectively). The lean mice were used as the non-diabetic normal control. TAWE was orally administrated for 6 weeks and the diabetic clinical markers, including blood glucose level, body weight, organs weight and insulin level were determined. The oral administration of TAWE-100 in ob/ob diabetic mice significantly decreased blood glucose level (78.4%) and body weight (11.9%) compared with diabetic control group. The weights of organs, including spleen, liver, kidneys, heart and lung were not different among groups, while the treatments of TAWE-100 in ob/ob diabetic mice significantly reduced blood total cholesterol (24.35%) and triglyceride (23.97%) levels compared with the diabetic control group. The levels of serum insulin and glucose tolerance were improved after TAWE-100 treatment in ob/ob diabetic mice. Moreover, the immunohistochemical staining for insulin detection in pancreatic islet $\beta$-cells expressed high level of insulin in TAWE-100 treated ob/ob mice. From the above results, the intake of TAWE may be effective in anti-hyperglycemia by the attenuation of glucose and lipid levels. TAWE-containing diets or drugs may be beneficial for controlling diabetes mellitus type 2 in human.

• Geophysics and Geophysical Exploration
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• v.3 no.1
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• pp.1-6
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• 2000

The 1 $km^2$ area studied is located in Sukchun-ri, Hwasung-koon, the southern part of Kyeonggi-do. Even though this site has been known as a contaminated area caused by seawater intrusions, geophysical and geochemical surveys have never been carried out at the site to determine the extent of the seawater contamination and to investigate whether the seawater intrusion is in progress. The purpose of this study is to determine the extent of seawater contamination and a preferred channel of the seawater intrusion using geophysical methods such as DC resistivity surveys with Schlumberger array and a dipole-dipole array. In order to determine whether the seawater intrusion is in progress in the area, DC resistivity monitoring with Schlumberger array was performed. According to the resistivity map obtained from the inversion of the resistivity data measured with Schlumberger array, the study area is divided into two districts as relatively lowly resistive (less than 30 ohm-m) and highly resistive (more than 30 ohm-m) areas. The distribution of the lowly resistive area is consistent with the distribution of the layer composed of clay minerals, and the resistivity of this layer decreases slowly as approaching to the old seashore. Hydrogeological analysis shows that the clay layer within a distance of about 200 m from the seashore has been already contaminated by sea-water and its electric conductivity is 8 times higher than that of the sand layer covered by the clay layer. According to the results of the 2-dimensional DC resistivity surveys with a dipole-dipole array, there are two preferred channels of the seawater intrusion in the site, and both the channels are in the NW-SE direction from the old seashore. The lowly resistive zone in the southern channel extends to a depth of 80 m. The DC resistivity monitoring with Schlumberger array was carried out along the preferred channel which has the low resistivity Bone (fracture zone) that extended to a depth of 80 m. The time series of apparent resistivity, measured at a distance of 260 m from the old coast line, fluctuates with a period of 12 hours. From these observations, it can be concluded that the seawater intrusion caused by tidal action is still in progress along the fractured zone interpreted by the DC resistivity surveys with a dipole-dipole array.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.1
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• pp.25-36
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• 2008

Eye localization means localization of the center of the pupils, and is necessary for face recognition and related applications. Most of eye localization methods reported so far still need to be improved about robustness as well as precision for successful applications. In this paper, we propose a robust eye localization method using multi-scale Gabor feature vectors without big computational burden. The eye localization method using Gabor feature vectors is already employed in fuck as EBGM, but the method employed in EBGM is known not to be robust with respect to initial values, illumination, and pose, and may need extensive search range for achieving the required performance, which may cause big computational burden. The proposed method utilizes multi-scale approach. The proposed method first tries to localize eyes in the lower resolution face image by utilizing Gabor Jet similarity between Gabor feature vector at an estimated initial eye coordinates and the Gabor feature vectors in the eye model of the corresponding scale. Then the method localizes eyes in the next scale resolution face image in the same way but with initial eye points estimated from the eye coordinates localized in the lower resolution images. After repeating this process in the same way recursively, the proposed method funally localizes eyes in the original resolution face image. Also, the proposed method provides an effective illumination normalization to make the proposed multi-scale approach more robust to illumination, and additionally applies the illumination normalization technique in the preprocessing stage of the multi-scale approach so that the proposed method enhances the eye detection success rate. Experiment results verify that the proposed eye localization method improves the precision rate without causing big computational overhead compared to other eye localization methods reported in the previous researches and is robust to the variation of post: and illumination.

• The korean journal of orthodontics
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• v.31 no.1 s.84
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• pp.71-83
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• 2001

The purpose of this study was to Prove that the medial edge epithelial cells covering the secondary palatal shelves were removed by apoptosis during palatal fusion. 12 mature female rats (Suprague-Dawley) were mated overnight with male rats and sacrificed on days 15.0, 16.5, 16.75, 17.0 of pregnancy. The embryos were removed from the uterus and the heads were embedded in paraffin. The paraffin blocks were sectioned and the sections were undergone H-E staining for general histologic feature and TdT staining for detection of apoptotic cells. The obtained results were as follows. k. In the section of 16.0 and 16.5 day embryos, the palatal shelves were prior to contact and no apoptotic cells wereobserved in the medial edge epithelium. At the initial contact of Palatal shelves, there was a few apoptotic cells in the fusing epithelium. 2. In the 16.75 day embryos, the samples that epithelial seams did not lost there continuity, apoptotic cells were rarely seen at the midline epithelial seam. In contrast, a lot of apoptotic cells were observed at epithelial triangles and the junction between palatal shelves and nasal septum. 3. In the 16,75 day embryos, the samples that epithelial seams lost their continuity and disrupted to epithelial islands, large number, of apoptotic cells were observed at epithelial islands and epithelial triangles. Some apoptotic cells were also observed at the oral, nasal epithelium near the midline. 4. In the 17.0 day embryos, most of epithelial islands were disappeared and mesenchymal confluence was achieved. Apoptotic cells were rarely observed in the mesenchymal tissue which replaced epithelial islands, but there were some apoptotic cells at the epithelial triangles, oral and nasal epithelium. From the results of the study, it was revealed that medial edge epithelial cells of fusing palate were removed by apoptosis. Apoptotic cells were found mainly in the disappearing midline epithelial seam and the oral and nasal epithelial triangles at some late stages of palatal fusion.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.663-674
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• 2019

Birds are effective ecological indicators but there is no national protocol in place to monitor population dynamics of forest birds in Korea. To support the establishment of future monitoring protocols, we compared the results of two generally used monitoring methods for forest bird surveys in two temperate mixed forests in central Korea. There was no statistical difference in the number of species and individuals detected per unit survey effort when comparing line transects and point counts. The number of species and individuals were higher in a five-minute count than in a three-minute point count, but the total accumulated number of expected observed species showed no difference between the two count durations. The number of observed species and individuals increased in both methods as plot radius or transect width increased, suggesting that multi-layer or multi-band surveys may be useful for quantitative and qualitative objectives. The decreasing number of observed species and individuals after sunrise suggested that bird monitoring should be conducted earlier in the morning, within four hours after sunrise. To detect 70% of the total number of species, 7.0 to 7.6 survey hours, equivalent to 42 three-minute counts (95% confidence interval [CI]: 26 to 61) or 33 five-minute counts (95% CI: 19 to 53) were needed for unlimited radius point counts. On the other hand, 4.8 survey hours, equivalent to 26 line transect counts (95% CI: 15 to 45) using 200-m transects with unlimited width, were required to achieve the same level of species detection. Therefore, the line transect method may be more effective than the point count method, at least in terms of local species richness assessment. For national forest bird monitoring, our data indicated that one or both survey methods can be selected as a basic protocol, based on the goals and scales of monitoring, forest types, and the conditions of the target areas.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.4
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• pp.459-464
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• 2017

Biphenyl is used as an intermediate in the production of crop protection products, a solvent in pharmaceutical production, and as a component in the preservation of citrus fruits in many countries. Biphenyl is not authorized for use and also does not have standards or specifications as a food additive in Korea. National and imported food products are likely to contain biphenyl. Therefore, control and management of these products is required. In this study, a simple analytical method was developed and validated using HPLC to determine biphenyl in food. These methods are validated by assessing certain performance parameters: linearity, accuracy, precision, recovery, limit of detection (LOD), and limit of quantitation (LOQ). The calibration curve was obtained from 1.0 to $100.0{\mu}g/mL$ with satisfactory relative standard deviations (RSD) of 0.999 in the representative sample (orange). In the measurement of quality control (QC) samples, accuracy was in the range of 95.8~104.0% within normal values. The inter-day and inter-day precision values were less than 2.4% RSD in the measurement of QC samples. Recoveries of biphenyl from spiked orange samples ranged from 92.7 to 99.4% with RSD between 0.7 and 1.7% at levels of 10, 50, and $100{\mu}g/mL$. The LOD and LOQ were determined to be 0.04 and $0.13{\mu}g/mL$, respectively. These results show that the developed method is appropriate for biphenyl identification and can be used to examine the safety of citrus fruits and surface treatments containing biphenyl residues.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.5
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• pp.646-652
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• 2017

The aim of this study was to investigate method validation for determination of sesamol, sesamin, and sesamolin in non-fermented sesame and fermented sesame by bioconversion. For validation, the specificity, linearity, precision, accuracy, limits of detection (LOD), and quantification (LOQ) of sesamol, sesamin, and sesamolin were measured by HPLC. Linearity tests showed that the coefficients of calibration correlation ($R^2$) for sesamol, sesamin, and sesamolin were 0.9999. Recovery rates of lignan contents in non-fermented and fermented sesame were high in the ranges of 100.27~115.10% and 98.43~114.90%, respectively. The inter-day and intra-day precisions of sesamin and sesamolin analyses for non-fermented and fermented sesame were 0.27~1.94% and 0.25~0.69%, respectively. The LOD and LOQ were $0.23{\sim}0.34{\mu}g/g$ and $0.70{\sim}1.03{\mu}g/g$, respectively. These results indicate that the validated method is appropriate for the determination of sesamol, sesamin, and sesamolin.