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Inherited metabolic diseases in the urine organic acid analysis of complex febrile seizure patients (복합 열성경련 환자의 소변 유기산 분석에서 나타난 유전대사질환)

  • Cheong, Hee Jeong;Kim, Hye Rim;Lee, Seong Soo;Bae, Eun Joo;Park, Won Il;Lee, Hong Jin;Choi, Hui Chul
    • Clinical and Experimental Pediatrics
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    • v.52 no.2
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    • pp.199-204
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    • 2009
  • Purpose : Seizure associated with fever may indicate the presence of underlying inherited metabolic diseases. The present study was performed to investigate the presence of underlying metabolic diseases in patients with complex febrile seizures, using analyses of urine organic acids. Method : We retrospectively analyzed and compared the results of urine organic acid analysis with routine laboratory findings in 278 patients referred for complex febrile seizure. Results : Of 278 patients, 132 had no abnormal laboratory findings, and 146 patients had at least one of the following abnormal laboratory findings: acidosis (n=58), hyperammonemia (n=55), hypoglycemia (n=21), ketosis (n=12). Twenty-six (19.7 %) of the 132 patients with no abnormal findings and 104 (71.2%) of the 146 patients with statistically significant abnormalities showed abnormalities on the organic acid analysis (P<0.05). Mitochondrial respiratory chain disorders (n=23) were the most common diseases found in the normal routine laboratory group, followed by PDH deficiency (n=2) and ketolytic defect (n=1). In the abnormal routine laboratory group, mitochondrial respiratory chain disorder (n=29) was the most common disease, followed by ketolytic defects (n=27), PDH deficiency (n=9), glutaric aciduria type II (n=9), 3-methylglutaconic aciduria type III (n=6), biotinidase deficiency (n=5), propionic acidemia (n=4), methylmalonic acidemia (n=2), 3-hydroxyisobutyric aciduria (n=2), orotic aciduria (n=2), fatty acid oxidation disorders (n=2), 2-methylbranched chain acyl CoA dehydrogenase deficiency (n=2), 3-methylglutaconic aciduria type I (n=1), maple syrup urine disease (n=1), isovaleric acidemia (n=1), HMG-CoA lyase deficiency (n=1), L-2-hydroxyglutaric aciduria (n=1), and pyruvate carboxylase deficiency (n=1). Conclusion : These findings suggest that urine organic acid analysis should be performed in all patients with complex febrile seizure and other risk factors for early detection of inherited metabolic diseases.

Control of Phythophthora capsici and Residual Characteristics by the Pesticides Tank-Mixed in Tomato Hydroponic Culture System (농약의 양액 탱크내 혼합처리에 의한 토마토 역병 방제 효과 및 잔류 특성)

  • Ihm, Yang-Bin;Kyung, Kee-Sung;Kim, Cban-Sub;Park, Byung-Jun;Lee, Jung-Sup
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.264-270
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    • 2003
  • To control effectively and safely Phytophthora root rot caused by Phytophthora capsici on tomato in hydroponic culture, tank-mixing method was considered with two pesticides, metalaxyl copper oxychloride 50% WP and dimethomorph dithianon 38% WP. Forty days after transplanting of tomato seedlings, 4 mL of sporangia of P. capsici (about 25 sporangi/mL) per plot was inoculated around tomato plant roots, and at 5 days after inoculation, the pesticides tank-mixed at three dilution levels, 12,500, 25,000 and 50,000, were drenched 1, 2 or 3 times per plot on the culture cube every 15 days for metalaxyl copper oxychloride 50% WP and every 10 days for dimethomorph dithianon 38% WP. During the drenching period, the residue levels of metalaxyl and dimethomorph in hydroponic culture solution were similar to the initial levels but the level of dithianon was drastically decreased from one day after tank-mixing. In tomato drenched with metalaxyl copper oxychloride 50% WP, metalaxyl was detected $0.02\sim0.04$ mg/kg in all diluted plots. Dimethomorph was detected $0.012\sim0.021$, $0.001\sim0.006$ and $0.001\sim0.003$ mg/kg in 12,500, 25,000 and 50,000 times diluted plots, respectively, while dithianon was detected 0.005, 0.003 mg/kg in 12,500 and 50,000 times diluted plots, respectively. The detection levels of three pesticides were far below compared with the levels of Korean MRLs. Incidences of Phytophthora root rot were not found in all the plots, but phytotoxic responses were recognized in the 12,500 times diluted plots of both pesticides. Based on the above results, the drenching of the culture solution tank-mixed with these pesticides could be recommended as a very safe and effective method to control Phytophthora root rot in tomato in hydroponic culture.

Monitoring of Pesticide Residues on Dried Agricultural Products (건조채소류의 잔류농약 실태 조사)

  • Gang, Gyungri;Mun, Sujin;Kim, Gwang-Gon;Yang, Yongshik;Lee, Semi;Choi, Euna;Ha, Dongryong;Kim, Eunsun;Cho, Baesik
    • The Korean Journal of Pesticide Science
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    • v.21 no.1
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    • pp.49-61
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    • 2017
  • The study was conducted for safety evaluation of 208 kinds of residue pesticides on 200 dried agricultural products, which are distributed in Gwangju. The method of monitoring was the second of Multi Class Pesticide Multi-residue Methods in Korean Food Code, and GC-ECD, GC-NPD, GC-MSD, and LC-MS/MS were used as evaluation equipment to analyze. The residue level in pesticides were 15.5% (31 of 200 samples) and 4 samples exceeded MRLs. 4.5 mg/kg of pyraclostrobin (MRL; 3.0 mg/kg) was detected in red pepper, 1.49 mg/kg of chlorpyrifos (MRL; 0.13 mg/kg) in daikon leaves, 38.26 mg/kg of pyridalyl (MRL; 0.25 mg/kg) in pepper leaves, 0.98 mg/kg of chlorpyrifos (MRL; 0.05 mg/kg), respectively. Pesticides were found on the 15 samples among the 21 samples of red pepper which is a fruit vegetable, and this resulted in high detection rate of 71%. In addition, pesticides were detected on chwinamul, shitake, siler divaricata, daikon leaves and others within MRLs. The frequent detected kinds of pesticides were insecticide (47.6%), fungicide (33.3%), acaricide (14.3%), nematicide (4.8%) in the order named, and pesticides were methoxyfenozide > pyraclostrobin > azoxystrobin, chlorantraniprole > novaluron, trifloxystrobin in frequent order.

Bacteriological Study of Sea Water and Oyster in Charan Bay, Korea (자란만의 해수 및 굴의 세균학적 연구)

  • CHOI Jong-Duck;JEONG Woo-geon;KIM Poong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.429-436
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    • 1998
  • A bacteriological study of sea water and oyster in Charan Bay was conducted to evaluate sanitary conditions of the bay and compliance of waters with the recommended bacteriological criteria for the designated area of shellfish cultivation, The Samples were collected at 23 sampling stations(Fig. 1 and Fig. 2) estaslished once a month from January 1997 to December 1997, During the study period, temperature ranged from 4.7 to $25.6^{\circ}C$, transparency ranged from 3.3 to 6.2m chemical oxygen demand ranged from 1.67 to 2.18 mg/$\ell$, dissolved oxygen demand ranged from 5.4 to 10.0 mg/$\ell$ dissolved nitrogen ranged from 1.65 to 7.88 $\mu$g-at/$\ell$, phosphate ranged from 0.15 to 1.16 $\mu$g-at/$\ell$, Chlorophylla-a ranged from 0.95 to 12.69mg/$\ell$. The coliform group and fecal coliform MPN's of sea water were ranged from <1.8$\~$l,600 and <1.8$\~$540, respectively. The coliform group and fecal coliform MPN's of oysters were ranged from <18$\~$16,000 and <18$\~$1,400, respectively. The viable cell counts in oyster ranged from $1.5\times10^2$ to $7.5\times10^3$. The bacteriological criteria of sea water in shellfish growing area should be less than 70 per 100 ml of sea water for median value of coliform MPN, and below $10\%$ of the samples which contain over than 230 for coliform MPN or over than 43 for fecal coliform MPN. The sea water from 432 samples were complied water coliform criteria recommended for designated shellfish growing area. The coliform group, fecal coliform, classification of coliform group with IMViC reactions and pathogenic vibrios were analyzed. During the study period, infectious bacteria such as Vibrio cholerae, Salmonella sp, and Shigella sp, were not detected from the samples, but detection ratios of Vibrio parahaemolyticus and Vibrio vulnifirus were $7\~17\%$ in summer months.

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SELECTIVE DETECTION OF VIABLE ENTEROCOCCUS FAECALIS USING PROPIDIUM MONOAZIDE IN COMBINATION WITH REAL-TIME PCR (Propidium monoazide와 real-time PCR을 이용한 살아있는 Enterococcus faecalis의 선택적인 검출)

  • Kim, Sin-Young;Lee, Seung-Jong;Kim, Eui-Seong;Seo, Deog-Gyu;Song, Yoon-Jung;Jung, Il-Young
    • Restorative Dentistry and Endodontics
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    • v.33 no.6
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    • pp.537-544
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    • 2008
  • Polymerase chain reaction (PCR) can detect bacteria more rapidly than conventional plate counting. However DNA-based assays cannot distinguish between viable and dead cells due to persistence of DNA after cells have lost their vitality. Recently, propidium monoazide (PMA) treatment has been introduced. The purpose of this study is to evaluate the applicability of the PMA treatment and real-time PCR method for cell counting in comparison with plate counting and to evaluate the antibacterial efficacy of 2% CHX on E. faecalis using PMA treatment in combination with real-time PCR. Firstly, to elucidate the relationship between the proportion of viable cells and the real-time PCR signals after PMA treatment, mixtures with different ratios of viable and dead cells were used. Secondly, relative difference of viable cells using PMA treatment in combination with real-time PCR was compared with CFU by plate counting. Lastly, antibacterial efficacy of 2% CHX on E. faecalis was measured using PMA treatment in combination with real-time PCR. The results were as follows : 1. Ct value increased with decreasing proportion of viable E. faecalis. 2. There was correlation between viable cells measured by real-time PCR after PMA treatment and CFU by plate counting until Optical density (OD) value remains under 1.0. However, viable cells measured by real-time PCR after PMA treatment have decreased at 1.5 of OD value while CFU kept increasing. 3. Relative difference of viable E. faecalis decreased more after longer application of 2% CHX.

Restoring Omitted Sentence Constituents in Encyclopedia Documents Using Structural SVM (Structural SVM을 이용한 백과사전 문서 내 생략 문장성분 복원)

  • Hwang, Min-Kook;Kim, Youngtae;Ra, Dongyul;Lim, Soojong;Kim, Hyunki
    • Journal of Intelligence and Information Systems
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    • v.21 no.2
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    • pp.131-150
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    • 2015
  • Omission of noun phrases for obligatory cases is a common phenomenon in sentences of Korean and Japanese, which is not observed in English. When an argument of a predicate can be filled with a noun phrase co-referential with the title, the argument is more easily omitted in Encyclopedia texts. The omitted noun phrase is called a zero anaphor or zero pronoun. Encyclopedias like Wikipedia are major source for information extraction by intelligent application systems such as information retrieval and question answering systems. However, omission of noun phrases makes the quality of information extraction poor. This paper deals with the problem of developing a system that can restore omitted noun phrases in encyclopedia documents. The problem that our system deals with is almost similar to zero anaphora resolution which is one of the important problems in natural language processing. A noun phrase existing in the text that can be used for restoration is called an antecedent. An antecedent must be co-referential with the zero anaphor. While the candidates for the antecedent are only noun phrases in the same text in case of zero anaphora resolution, the title is also a candidate in our problem. In our system, the first stage is in charge of detecting the zero anaphor. In the second stage, antecedent search is carried out by considering the candidates. If antecedent search fails, an attempt made, in the third stage, to use the title as the antecedent. The main characteristic of our system is to make use of a structural SVM for finding the antecedent. The noun phrases in the text that appear before the position of zero anaphor comprise the search space. The main technique used in the methods proposed in previous research works is to perform binary classification for all the noun phrases in the search space. The noun phrase classified to be an antecedent with highest confidence is selected as the antecedent. However, we propose in this paper that antecedent search is viewed as the problem of assigning the antecedent indicator labels to a sequence of noun phrases. In other words, sequence labeling is employed in antecedent search in the text. We are the first to suggest this idea. To perform sequence labeling, we suggest to use a structural SVM which receives a sequence of noun phrases as input and returns the sequence of labels as output. An output label takes one of two values: one indicating that the corresponding noun phrase is the antecedent and the other indicating that it is not. The structural SVM we used is based on the modified Pegasos algorithm which exploits a subgradient descent methodology used for optimization problems. To train and test our system we selected a set of Wikipedia texts and constructed the annotated corpus in which gold-standard answers are provided such as zero anaphors and their possible antecedents. Training examples are prepared using the annotated corpus and used to train the SVMs and test the system. For zero anaphor detection, sentences are parsed by a syntactic analyzer and subject or object cases omitted are identified. Thus performance of our system is dependent on that of the syntactic analyzer, which is a limitation of our system. When an antecedent is not found in the text, our system tries to use the title to restore the zero anaphor. This is based on binary classification using the regular SVM. The experiment showed that our system's performance is F1 = 68.58%. This means that state-of-the-art system can be developed with our technique. It is expected that future work that enables the system to utilize semantic information can lead to a significant performance improvement.

A Collaborative Video Annotation and Browsing System using Linked Data (링크드 데이터를 이용한 협업적 비디오 어노테이션 및 브라우징 시스템)

  • Lee, Yeon-Ho;Oh, Kyeong-Jin;Sean, Vi-Sal;Jo, Geun-Sik
    • Journal of Intelligence and Information Systems
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    • v.17 no.3
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    • pp.203-219
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    • 2011
  • Previously common users just want to watch the video contents without any specific requirements or purposes. However, in today's life while watching video user attempts to know and discover more about things that appear on the video. Therefore, the requirements for finding multimedia or browsing information of objects that users want, are spreading with the increasing use of multimedia such as videos which are not only available on the internet-capable devices such as computers but also on smart TV and smart phone. In order to meet the users. requirements, labor-intensive annotation of objects in video contents is inevitable. For this reason, many researchers have actively studied about methods of annotating the object that appear on the video. In keyword-based annotation related information of the object that appeared on the video content is immediately added and annotation data including all related information about the object must be individually managed. Users will have to directly input all related information to the object. Consequently, when a user browses for information that related to the object, user can only find and get limited resources that solely exists in annotated data. Also, in order to place annotation for objects user's huge workload is required. To cope with reducing user's workload and to minimize the work involved in annotation, in existing object-based annotation automatic annotation is being attempted using computer vision techniques like object detection, recognition and tracking. By using such computer vision techniques a wide variety of objects that appears on the video content must be all detected and recognized. But until now it is still a problem facing some difficulties which have to deal with automated annotation. To overcome these difficulties, we propose a system which consists of two modules. The first module is the annotation module that enables many annotators to collaboratively annotate the objects in the video content in order to access the semantic data using Linked Data. Annotation data managed by annotation server is represented using ontology so that the information can easily be shared and extended. Since annotation data does not include all the relevant information of the object, existing objects in Linked Data and objects that appear in the video content simply connect with each other to get all the related information of the object. In other words, annotation data which contains only URI and metadata like position, time and size are stored on the annotation sever. So when user needs other related information about the object, all of that information is retrieved from Linked Data through its relevant URI. The second module enables viewers to browse interesting information about the object using annotation data which is collaboratively generated by many users while watching video. With this system, through simple user interaction the query is automatically generated and all the related information is retrieved from Linked Data and finally all the additional information of the object is offered to the user. With this study, in the future of Semantic Web environment our proposed system is expected to establish a better video content service environment by offering users relevant information about the objects that appear on the screen of any internet-capable devices such as PC, smart TV or smart phone.

Extracting Beginning Boundaries for Efficient Management of Movie Storytelling Contents (스토리텔링 콘텐츠의 효과적인 관리를 위한 영화 스토리 발단부의 자동 경계 추출)

  • Park, Seung-Bo;You, Eun-Soon;Jung, Jason J.
    • Journal of Intelligence and Information Systems
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    • v.17 no.4
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    • pp.279-292
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    • 2011
  • Movie is a representative media that can transmit stories to audiences. Basically, a story is described by characters in the movie. Different from other simple videos, movies deploy narrative structures for explaining various conflicts or collaborations between characters. These narrative structures consist of 3 main acts, which are beginning, middle, and ending. The beginning act includes 1) introduction to main characters and backgrounds, and 2) conflicts implication and clues for incidents. The middle act describes the events developed by both inside and outside factors and the story dramatic tension heighten. Finally, in the end act, the events are developed are resolved, and the topic of story and message of writer are transmitted. When story information is extracted from movie, it is needed to consider that it has different weights by narrative structure. Namely, when some information is extracted, it has a different influence to story deployment depending on where it locates at the beginning, middle and end acts. The beginning act is the part that exposes to audiences for story set-up various information such as setting of characters and depiction of backgrounds. And thus, it is necessary to extract much kind information from the beginning act in order to abstract a movie or retrieve character information. Thereby, this paper proposes a novel method for extracting the beginning boundaries. It is the method that detects a boundary scene between the beginning act and middle using the accumulation graph of characters. The beginning act consists of the scenes that introduce important characters, imply the conflict relationship between them, and suggest clues to resolve troubles. First, a scene that the new important characters don't appear any more should be detected in order to extract a scene completed the introduction of them. The important characters mean the major and minor characters, which can be dealt as important characters since they lead story progression. Extra should be excluded in order to extract a scene completed the introduction of important characters in the accumulation graph of characters. Extra means the characters that appear only several scenes. Second, the inflection point is detected in the accumulation graph of characters. It is the point that the increasing line changes to horizontal line. Namely, when the slope of line keeps zero during long scenes, starting point of this line with zero slope becomes the inflection point. Inflection point will be detected in the accumulation graph of characters without extra. Third, several scenes are considered as additional story progression such as conflicts implication and clues suggestion. Actually, movie story can arrive at a scene located between beginning act and middle when additional several scenes are elapsed after the introduction of important characters. We will decide the ratio of additional scenes for total scenes by experiment in order to detect this scene. The ratio of additional scenes is gained as 7.67% by experiment. It is the story inflection point to change from beginning to middle act when this ratio is added to the inflection point of graph. Our proposed method consists of these three steps. We selected 10 movies for experiment and evaluation. These movies consisted of various genres. By measuring the accuracy of boundary detection experiment, we have shown that the proposed method is more efficient.

New Analytical Method to Identify Chromium Species, Cr(III) and Cr(VI), and Characteristic Distribution of Chromium Species in the Han River (한강수계해서의 크롬(III,VI) 종(species) 분포 및 분석방법 정립)

  • Jeong, Gwan-Jo;Kim, Dok-Chan;Park, Hyeon
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.6
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    • pp.590-598
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    • 2005
  • An adequate method to identify chromium separation, Cr(III) and Cr(VI), in water samples were studied by using High Performance Liquid Chromatography(HPLC) coupled with Inductively Coupled Plasma Mass Spectometer(ICP-MS) equipped with Dynamic Reaction Cell(DRC). The characteristic distribution of Cr(III) and Cr(VI) in the raw water taken at the six water intake stations in Seoul, was analyzed by the method developed by the authors. The chromium species separated by HPLC was isocratically conducted by using tetrabutylammonium phosphate monobasic(1.0 mM TBAP), ethylenediaminetetraacetic acid(0.6 mM EDTA) and 2% v/v methanol as the mobile phase. 5% v/v methanol was used as flushing solvent. A reactive ammonia($NH_3$) gas was used to eliminate the potential interference of $ArC^+$. Several Parameters such as solvent ratio, pH, flow rate and sample injection volume were optimized for the successful separation and reproducibility. Although it has been reported thai the separation sensitivity of Cr(III) is superior to that of Cr(VI), the authors observed Cr(VI) was more sensitive than Cr(III) when ammonia($NH_3$) gas was used as the reaction gas. It took less than 3 minutes to analyze chromium species with this method and the estimated detection limits were $0.061\;{\mu}g/L$ for Cr(III) and $0.052\;{\mu}g/L$, for Cr(VI). According to the results from the analysis on chromium species in the raw water of the six intake stations, the concentrations of Cr(III) ranged from 0.048 to $0.064\;{\mu}g/L$(ave. $0.054\;{\mu}g/L$) while that of Cr(VI) ranged from 0.014 to $0.023\;{\mu}g/L$(ave. $0.019\;{\mu}g/L$). Recovery ratio was very high($90.1{\sim}94.1%$). There were two or three times more Cr(III) than Cr(VI) in the raw water.

The Consideration about Heavy Metal Contamination of Room and Worker in a Workshop (공작실에서 실내 및 작업종사자의 중금속 오염도에 관한 고찰)

  • Kim Jeong-Ho;Kim Gha-Jung;Kim Sung-Ki;Bea Suk-Hwan
    • The Journal of Korean Society for Radiation Therapy
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    • v.17 no.2
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    • pp.87-94
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    • 2005
  • Purpose : Heavy metal use when producing the block from the workshop. At this time, production of heavy metal dust and fume gives risk in human. This like heavy metal to improve seriousness through measurement and analysis. And by the quest in solution is purpose of this thesis. Materials and Methods : Organization is Inductively Coupled Plasma Atomic Emission Spectrometer, and the object is Deajeon city 4 workshops in university hospital radiation oncology (Bismuth, Lead, Tin and cadmium). Method is the ppb the pumping it does at unit, comparison analysis. And the Calculation heavy metal standard level in air through heavy metal standard level in body and blood, so Heavy metal temporary standard set. Results : Subterranean existence room air quality the administration laws appointed Lead and Cadmium's exposure recommend that it is $3{\mu}g/m^3\;and\;2{\mu}g/m^3$. And Bismuth and Tin decides $7{\mu}g/m^3\;and\;6{\mu}g/m^3$ through standard level in air heavy metal and standard level in body and blood. Heavy metal measurement level of workshops in 4 university hospital Daejeon city compares with work existence and nonexistence. On work nonexistence almost measurement level is below the recommend level. But work existence case express high level. Also consequently in composition ratio of the block is continuous with the detection ratio. Conclusion : Worker's heavy metal contamination imbrued serious for solution founds basic part. In hospital may operation on local air exhauster and periodical efficiency check, protector offer, et al. And worker have a correct understanding part of heavy metal contamination, and have continuous interest, health control. Finally, learned society sphere administer to establishment standard level and periodical measurement. And it founds basic solution plan of periodical special health checkup.

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