• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.139.1-139
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• 2003

Tissues from woody plant contain higher amount of phenolic compounds and polysaccharides, which give inhibitory effects on reverse transcriptase and/or Taq ploymerase. The common multiple-step protocols using several additives to inhibit polyphenoic compounds during nucleic acid extraction are time consuming and laborious. Sodium sulfite (Na$_2$SO$_3$) was used as inhibitor of polyphenolic oxidases in extraction buffer and compare it's effect between commercial RNA extraction kit and small-scale double-stranded RNA (dsRNA) extraction by RT-PCR. During nucleic acid extraction procedure, addition of 0.5％-1.5％ (w/v) sodium sulfite to Iysis buffer or STE buffer resulted in lighter color change than extracts without sodium sulfite and improve the RT-PCR detection. When commercial RNA extraction kit used, optimal concentration of sodium sulfite were variable according to the host plant. However, using dsRNA as RT-PCR template, 1.5％ sodium sulfite in STE buffer improves the detection of both viruses and unspecific amplifications were reduced significantly, Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

• Journal of Chest Surgery
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• 제32권2호
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• pp.151-157
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• 1999

배경: 많은 실험적인 연구에서 종양 조직 내의 아포프토시스와 미세 혈관의 생성은 서로 반비례한다고 보고된다. 비소세포 폐암 조직내에서 두 수치의 관계를 조사하여 보았다. 대상 및 방법:조직내의 아포프토시스의 정도는 deoxynucleotidyl trasferase방법으로(Apop Tag In Situ Apoptosis Detection Kit, ONCOR) 측정하였고, 종양내 미세 혈관 밀도는 항 CD 31 항체를 이용하였다. 결과:아포프토시스 지수와 종양내 미세 혈관 밀도 사이에는 통계적으로 유의하게 역 상관관계가 있었다(p = 0.047). 결론: 비소세포 폐암종에서 아포프토시스와 미세 혈관 생성의 정도는 서로 연관이 있다고에 할 수있다. 그리고 종양내의 신생 혈관의 생성이 종양내 아포프토시스의 억제에 기여한다고 유추 할 수 있다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2009년도 춘계학술대회
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• pp.661-664
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• 2009

최근 지능형 감시카메라에 대한 연구가 활발히 진행되고 있다. 그러나 현재의 연구들은 대부분 통합시스템 구현보다는 단일 모듈에 대한 성능향상에 중점을 두고 있다. 따라서 본 논문에서는 이동이 가능한 몸체, 얼굴검출, 모션 센서 통합 모듈로 구성하는 이동형 무선감시 시스템을 구현하였다. 이동형 무선 감시 시스템의 구현에는 sharp사의 카메라 모듈과 ecom사의 무선영상전송 모듈, A4WD1 Combo kit for RC를 이용한 이동로봇 바디, roboblock의 ZigBee RF 무선컨트롤 송수신 모듈을 사용 하였고, 모션 센서 모듈에는 PANASONIC의 AMN14111를 사용하였다. OpenCV 라이브러리를 이용한 얼굴검출과 MFC로 소프트웨어를 구현하였다. 본 논문에서 구현한 시스템은 모션 센서를 이용하는 이동형 영상 감시 시스템이나 얼굴검출이 필요한 시스템, 원격조정이 필요한 작업환경에 유용하게 사용될 수 있다.

• Journal of Electrical Engineering and Technology
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• 제4권1호
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• pp.143-148
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• 2009

About 70% of Korea consists of mountainous areas, and during the construction of many roads and railroads, cut slopes are inevitably formed. The rainy season, frost heaving in winter, and thawing in spring can all cause rockfalls and landslides. The failure of these slopes is increasing every year, causing damage to vehicles, personal injury and even death. To protect people and property from such damage, a real-time monitoring system is needed to detect the early stages of slope failures. The GMG placed TRS sensor units in the slopes to monitor them in real-time. But due to its reliance on data lines and power lines, the system is vulnerable to lightning damage. The whole system can be damaged by a single lighting strike. Consequently, for the purposes of this paper we propose the use of the Ubiquitous Sensor Network (USN) which follows the IEEE 802.1.4. By using the USN system we can minimize lightning damage and can monitor the movement of the slopes consistently.

• 대한수의학회지
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• 제42권4호
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• pp.545-550
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• 2002

The raccoon dog (Nyctereutes procyonoides) may be infected by Dirofilaria immitis. However, there has been no report on dirofilarial infection in the raccoon dog in Korea. In this study, we report on D. immitis infection in two wild raccoon dogs captured in the Daejeon area. The two raccoon dogs were referred to the Veterinary Teaching Hospital, Chungnam National University for diagnosis of D. immitis infection. The modified Knott's test for the detection of blood D. immitis microfilariae was positive, and serological test (FASTest$^{(R)}$ HW Antigen ELISA kit, Diagnostik Mega Cor, Austria) for D. immitis was positive as well. Additionally, D. immitis microfilariae were differentiated from other microfilariae by using acid phrnphatase histochemical staining (Leucognost-SP$^{(R)}$kit, Diagncstica MERCK, Germany). The two raccoon dogs were necropsed and D. immitis infection was confirmed.

• Parasites, Hosts and Diseases
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• 제57권1호
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• pp.27-31
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• 2019

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection $Kit^{(R)}$, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

• 한국재난정보학회 논문집
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• 제10권3호
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• pp.452-457
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• 2014

화학재난 발생시 현장대응 요원들은 사건 원인물질의 성상과 잔류오염 농도를 신속 정확하게 파악해야 한다. 또한 화학재난 현장에서의 적절한 대응절차 진행을 위해서는 화학물질의 성상과 오염농도 확인은 필수적이다. 이를 위해 현장에서 사용하는 각 장비의 특징을 알아보고자 한다. 현장대응장비는 모든 화학물질을 확인할 수 없으며, 각 장비별로 물질탐지에 제한적이다. 장비별 물질탐지 범위와 상호보완성을 고려해야 한다. 본 연구에서는 현장 활용장비인 간이탐지 킷과 검지관식 탐지장비, 전자식 탐지장비의 신속한 현장 활용을 위한 대응절차를 마련하여 현장대응에 도움을 주고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권3호
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• pp.299-302
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• 1996

Milk samples(n = 78) were taken 19d, 20d, 21d, 22d after artificial insemination(AI) for early pregnancy diagnosis by using the Enzyme immunoassay(EIA) kit. The progesterone ($P_4$) concentration in the whole milk was measured on the same day of pregnancy diagnosis. Rectal palpation(RP) was accomplished between 60d and 70d after AI to estimate the ovary condition and pregnancy status. Milk progesterone concentrations measured by Radio-immunoassay(RIA) method, in the pregnant cows at 17d, 19d, 21d after insemination were $17.10{\pm}0.91$, $17.60{\pm}0.46$, and $18.43{\pm}0.79nmol/l$, whereas those in the not-pregnant cows were $6.57{\pm}1.03$, $2.63{\pm}0.29$, and $0.67{\pm}0.08nmol/l$, respectively. When the progesterone concentration was less than 7 nmol/l, the color of the EIA kit was lighter and when the progesterone concentration was ${\geq}16nmol/l$, the color of the EIA kit was darker compared to the standard color. The detection rates of error by judging the color differences were 5.1% and 20.7%, respectively. In the early pregnancy diagnosis by the EIA kit and RIA method, the accuracy rates in the pregnancy of cows were 82% and 87%, and those in not-pregnant cows were 86% and 91%, respectively. For ovarian status estimated by the RIA method and certified by RP, the accuracy rates of the ovarian atrophy, follicular cyst and luteal cyst were 80, 91 and 83% and the progesterone concentrations were 2.51, 2.03, and 26.7 nmol/l, respectively.

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.375-380
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• 2016

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

• The Plant Pathology Journal
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• 제20권2호
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• pp.142-146
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• 2004

Woody plant tissues contain great amounts of phenolic compounds and polysaccharides. These substances inhibit the activation of reverse transcriptase and/or Taq polymerase in RT-PCR. The commonly used multiple-step protocols using several additives to diminish polyphenolic compounds during nucleic acid extraction are time consuming and laborious. In this study, sodium sulfite was evaluated as an additive for nucleic acid extraction from woody plants and the efficiency of RT-PCR assay of commercial nucleic acid extraction kits and small-scale dsRNA extraction was compared. Sodium sulfite was used as an inhibitor against polyphenolic oxidases and its effects were compared in RNA extraction by commercial extraction kit and small-scale double-stranded RNA (dsRNA) extraction method for RT-PCR. During nucleic acid extraction, addition of 0.5%-1.5%(w/v) of sodium sulfite to lysis buffer or STE buffer resulted in lighter browning by oxidation than extracts without sodium sulfite and improved the RT-PCR detection. When commercial RNA extraction kit was used, optimal concentrations of sodium sulfite were variable according to the tested plant. However, with dsRNA as RT-PCR template, sodium sulfite 1.5% in STE buffer improved the detection efficiency of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in fruit trees, and reduced the unspecific amplifications signi-ficantly. Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.