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한우 송아지의 설사 분변에서 Escherichia coli(K99), Clostridium perfringens와 Cryptosporidium parvum의 검출 (Detection of Escherichia coli(K99), Clostridium perfringens and Cryptosporidium parvum in Diarrhetic Feces of Korean Native Calves)

  • 전승기;이한경;김남수
    • 한국임상수의학회지
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    • 제24권4호
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    • pp.588-592
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    • 2007
  • The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.

순차적 결함 검출 방법에 기반한 TFT-LCD 결함 영역 검출 (TFT-LCD Defect Blob Detection based on Sequential Defect Detection Method)

  • 이은영;박길흠
    • 한국산업정보학회논문지
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    • 제20권2호
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    • pp.73-83
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    • 2015
  • 본 논문에서는 순차적 결함 검출 방법을 이용하여 TFT-LCD의 결함 영역(Blob)을 효과적으로 검출하는 알고리즘을 제안한다. 먼저 결함과 배경 간의 휘도 차를 이용하여 영상의 각 화소들에 대한 결함 확률을 판단하고, 결함 확률에 따른 순차적 결함 검출 방법을 이용하여 결함 후보 화소를 검출한다. 여기서 결함 확률이란 결함 후보 화소가 검출된 단계에 따라 결함 영역에 포함될 가능성을 나타내다. 형태학 연산을 적용함으로써 검출된 후보 화소들을 후보 결함 영역으로 형성하고, 각 후보 결함 영역에 대한 결함 가능성을 계산하여 결함 영역을 검출한다. 모의 TFT-LCD 영상을 생성하여 제안 방법의 타당성을 검증하고, 실제 TFT-LCD 영상에 적용함으로서 제안 알고리즘의 우수한 결함 검출 성능을 확인하였다.

Solid-Phase Microextraction에 의한 백련의 휘발성 향기 성분 분석 (Volatile Flavor Composition of White-flowered Lotus by Solid-phase Microextraction)

  • 최향숙
    • 한국식품영양학회지
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    • 제30권2호
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    • pp.363-370
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    • 2017
  • This study investigated the chemical composition of headspace gas from white-flowered lotus (Nelumbo nucifera Gaertner). Volatile flavor compositions of headspace from white-flowered lotus (floral leaf, stamen, flower stalk, stem) were investigated through the solid-phase microextraction method using polydimethylsiloxane-divinylbenzene fiber. The headspace was directly transferred to a gas chromatography-mass spectrometry. Sixty-three volatile flavor constituents were detected in the headspace of lotus floral leaves, and undecanoic acid (7.81%) was the most abundant component. Fifty-three volatile flavor constituents were detected in the headspace of lotus stamina, and isobutylidene phthalide (7.94%) was the most abundant component. Forty-four volatile flavor constituents were detected in the headspace of lotus flower stalks, and 3-butyl dihydrophthalide (11.23%) was the most abundant component. Fifty-nine volatile flavor constituents were detected in the headspace of lotus stems, and ligustilide (16.15%) was the most abundant component. The content of phthalides was higher in the headspace of flower stalks and stems, while alcohols and acids were the predominant compounds in lotus floral leaves.

곡류 가공품중의 미생물 오염도 조사 (Distribution of Microorganisms in Seonsik and Saengsik)

  • 김중범;박용배;강정복;김종찬
    • 환경위생공학
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    • 제20권1호
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    • pp.12-22
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    • 2005
  • This study was performed to survey distribution of microorganisms and food-borne pathogenic bacterium in order to estimate microbiological safety in seonsik and saengsik. Total aerobic bacteria was detected over $10^5\;CFU/g$ in raw materials($4.3\%$) and products($35.7\%$) of saengsik. Coliforms were detected over $10^2\;CFU/g$ in seonsik products($27.3\%$) and in raw materials($4.3\%$) and products($35.7\%$) of saengsik. Cl. perfringens was detected in saengsik products($4.8\%$). B. cereus was detected in raw materials($12.5\%$) and products($18.2\%$) of seonsik and raw materials($13.0\%$) and products($23.8\%$) of saengsik. Concentration and detection rate of microorganisms in products were higher than raw materials. These results show some food hygiene problems but do not cause food poisoning because concentration of Clostridium perfringens and B. cereus were lower than $10^5\;CFU/g$.

개 디스템퍼 바이러스의 신속검출법 개발 (Development of Rapid Detection Technique for Canine Distemper Virus)

  • 김두;안소저;권혁무
    • 한국임상수의학회지
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    • 제17권1호
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    • pp.13-20
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    • 2000
  • Recently cases of canine distemper have occurred in Korea despite vaccination was carried out nationwidely. This study was performed to establish rapid diagnosis of canine distemper by RT-PCR, nested PCR, and serological test. A total of 30 dogs, which were suspected canine distemper clinically, was examined. RT-PCR and nested PCR were specific for the amplification of CDV H gene and sensitive to detect 7 TCID50 of Onderstepoort strain. By RT-PCR, H gene was detected in 6(20%) of 30 peripheral bloods from dogs. And H gene was detected in 10(33.3%) of 30 samples by nested PCR. H gene was detected from 1 brain of 6 years-old Beagle dog and 1 lung of 2 months-old Shihtzu dog, in which peripheral blood H gene was not detected. Serum neutralizing antibody titer against Onderstepoort strain ranged from 4 to 1,024 in 30 patients. No correlation was observed between the results of nested PCR and titiers of neutralizing antibody.

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Sea fog detection near Korea peninsula by using GMS-5 Satellite Data(A case study)

  • Chung, Hyo-Sang;Hwang, Byong-Jun;Kim, Young-Haw;Son, Eun-Ha
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
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    • pp.214-218
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    • 1999
  • The aim of our study is to develop new algorism for sea fog detection by using Geostational Meteorological Satellite-5(GMS-5) and suggest the techniques of its continuous detection. So as to detect daytime sea fog/stratus(00UTC, May 10, 1999), visible accumulated histogram method and surface albedo method are used. The characteristic value during daytime showed A(min) > 20% and DA < 10% when visble accumulated histogram method was applied. And the sea fog region which detected is of similarity in composite image and surface albedo method. In case of nighttime sea fog(18UTC, May 10, 1999), infrared accumulated histogram method and maximum brightness temperature method are used, respectively. Maximum brightness temperature method(T_max method) detected sea fog better than IR accumulated histogram method. In case of T_max method, when infrared value is larger than T_max, fog is detected, where T_max is an unique value, maximum infrared value in each pixel during one month. Then T_max is beneath 700hpa temperature of GDAPS(Global Data Assimilation and Prediction System). Sea fog region which detected by T_max method was similar to the result of National Oceanic and Atmosheric Administration/Advanced Very High Resolution Radiometer (NOAA/AVHRR) DCD(Dual Channel Difference). But inland visibility and relative humidity didn't always agreed well.

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약사에 의해 탐지된 항암제 처방오류 분석 (Analysis of Chemotherapy Prescribing Errors Detected by Pharmacists)

  • 이현주;양미경;조주희;김성은;석현주;김현아
    • 한국임상약학회지
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    • 제20권2호
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    • pp.120-127
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    • 2010
  • Objective: The purpose of this study was to identify the type and frequency of chemotherapy-related prescribing errors and assess the pharmacist intervention in preventing potential harm. Methods: This study was performed in satellite pharmacy of oncology/hematology unit in tertiary teaching hospital from April to September, 2009. All chemotherapy prescribing errors detected by pharmacists were recorded. Frequency and characteristics of prescribing errors were analyzed. Pharmacists reviewed 28, 495 chemotherapy orders from 12,719 patients during 6-month periods. Results: A total of 835 prescription errors (2.93%) in 734 patients (5.77%) were detected by pharmacists. Alkylating agents (37.6%) followed by antimetabolite (23.35%), and mitotic inhibitors (21.44%) were the most prevalent classes in which errors occurs. The most common types of error detected were incorrect dose (34%), incorrect solution (33%), incorrect route (9%) and omission errors (8%). Changes in chemotherapy order due to pharmacists' intervention occurred in all error cases. Conclusion: Pharmacists' intervention in reviewing chemotherapy and drug orders intercepted potential harm due to prescribing errors. The current study provided strategies for reduction of medication errors.

광양만 저질에서의 미량오염물질 분포특성에 관한 연구 (A Study on Distribution Property of Micropollutants in Gwang-Yang Bay Sediment)

  • 한상국;박지영
    • 한국환경과학회지
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    • 제15권7호
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    • pp.643-651
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    • 2006
  • This study investigated the distributive property of micropollutants in sediment around Gwang-yang bay using simultaneous analytical method of 310 chemicals. In the results, the major micropollutants detected were CH type chemicals such as aliphatic, polycyclic compounds, benzenes, and CHN(O) type chemicals aromatic amines, and pesticides. Insecticides of pesticide type were frequently detected at all sampling site. The total concentration of micropollutants were higher in summer than in winter and measured within the range of $ND{\sim}36.50{\mu}g/L$. Also, because of effect of seomjin river, GY6 of all sampling site detected by the highest concentration, GY10 and 11 located in Gwang-yang outside bay were not detected the micropollutants. From the result of this study, we should estimated that the industrial complex located in Gwang-yang inside bay and an inflow of fresh water through the Seomjin river are major pollution sources of Gwang-yang bay.

Polymerase Chain Reaction-Sequence Specific Primer를 이용한 HLA-B 유전자의 DNA 다형성 조사 (Genotyping of HLA-B by Polymerase Chain Reaction-Sequence Specific Primer)

  • 장순모
    • 대한임상검사과학회지
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    • 제39권3호
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    • pp.147-150
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    • 2007
  • Most expressed HLA (human leukocyte antigen) loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this study, the HLA-B genotypes were determined in twenty students unrelated koreans using the PCR-SSP (polymerase chain reaction-sequence specific primer) technique. Several specific primer pairs in assigning the HLA-B gene were used ($B^{\ast}4001/4007$, $B^{\ast}4901/5001/4501$, $B^{\ast}3701$, $B^{\ast}5801$). The results of PCR-SSP, the HLA-B3701 primer was detected one (5%), the $HLA-B^{\ast}5801$ were detected four (20%), the $HLA-B^{\ast}4001/4007$ were detected nineteen (95%) and the $HLA-B^{\ast}4901/5001/4501$ were detected twenty. This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-B genotypes. Moreover, these results genotype frequency of the HLA-B gene could be useful for database study before being applied to individual identification and transplantation immunity.

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퍼지추론을 이용한 얼굴영역 검출 알고리즘 (Face Region Detection Algorithm using Fuzzy Inference)

  • 정행섭;이주신
    • 한국항행학회논문지
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    • 제13권5호
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    • pp.773-780
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    • 2009
  • 본 논문은 픽셀의 색상과 채도를 퍼지추론한 얼굴영역 검출 알고리즘을 제안하였다. 제안한 알고리즘은 조명보정과 얼굴 검출 과정으로 구성되었다. 조명보정 과정에서는 조명변화에 대한 보정기능을 수행한다. 얼굴 검출 과정은 20개의 피부 색상 모델에서 계산된 색상과 채도를 특징 파라미터로 멤버쉽 함수를 생성하여 유사도를 평가하였다. 추출된 얼굴 후보영역을 CMY칼라 모델에서 C요소로 눈을 검출하였고, YIQ 칼라 공간에서 Q요소로 입을 검출하였다. 추출된 얼굴 후보영역에서 일반적인 얼굴에 대한 지식을 기반으로 얼굴 영역을 검출하였다. 입력받은 정면 칼라 영상으로 실험한 결과, 얼굴 영상의 위치와 크기에 관계없이 얼굴 영역이 검출됨을 알 수 있었다.

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