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Detailed morphological analysis of axolotl sperm

  • Keskin, Ilknur;Gurgen, Duygu Gursoy;Avinca, Didem;Ozdemir, Ekrem Musa;Keskin, Suat Utku;Karabulut, Seda
    • Korean Journal of Veterinary Research
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    • v.61 no.3
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    • pp.25.1-25.7
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    • 2021
  • The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 ㎛, 356.544 ㎛, and 103.661 ㎛, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.

Implementation of Mapping Function for 400 Gigabit Flexible Ethernet Signal in OTN (OTN에서의 400Gb/s급 Flexible 이더넷 신호수용 위한 맵핑 기능 구현)

  • Lee, Chang-Ki
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.12 no.3
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    • pp.257-264
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    • 2019
  • Recently, ITU-T recommends that FlexE, which allows the flexible configuration of Ethernet signals, be received by OTN for transparent transmission through OTN. To compensate for the difference in bit rate that can occur when mapping FlexE signals to OTN payload, an idle codeword is removed or inserted. However, the detailed functional blocks required to implement this method are not yet available. In this paper, based on the recent ITU-T requirements, a detailed functional block for OTN mapping of FlexE signals is proposed based on 400G class. In addition, based on the detailed functional blocks, mathematical analysis was performed to obtain the characteristics of removing and inserting idle code words, and the simulation results are shown.

A Study on the Improvement of Standards of Traffic Information Service and Provide Services Based on the Detailed Traffic Information (교통정보서비스 표출기준 개선 및 상세교통정보 기반 서비스 제공방안 연구)

  • Bae, Kwangsoo;Lee, Seungcheol
    • Journal of Information Technology Services
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    • v.17 no.4
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    • pp.85-100
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    • 2018
  • In this study, we formulated rational criteria to efficiently provide traffic information services via a crafted approach. By utilizing this, we presented a detailed traffic information service providing method that can overcome the limitations of existing link unit information provision system. Three methodologies such as user survey, data mining, and KHCM (Korea Highway Capacity Manual) utilization method were applied to formulate a rational expression standard for traffic information service. Each method was designed to establish a quantitative criterion for various traffic conditions and to enable user-oriented traffic information service in consideration of the traffic principal/compatibility. Considering the results of each methodological analysis in a comprehensive manner, the basic expression standards for traffic information service was formulated. Then we presented improvements such as traffic condition step by road, speed range of traffic condition, expression term of traffic condition and so on. In order to complement the problems of the information provision system of the existing link unit based on the derived improvement criterion, we presented the detailed traffic information service provision method by using the traffic speed data of the second order. And we applied this to the two links of Daegu city. The method presented in this research can improve the quality of traffic information service. Not only it can be used for various fields such as optimal route search, traffic safety service and so on.

Analysis of dental hygiene learning objectives based on Bloom's taxanomy (Bloom의 교육목표 분류에 기반한 치위생학 학습목표 분석)

  • Ki, Ji-Yun;Jang, Jong-Hwa
    • Journal of Korean society of Dental Hygiene
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    • v.21 no.2
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    • pp.193-201
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    • 2021
  • Objectives: We evaluated the learning objectives of dental hygiene courses based on Bloom's learning objectives, and analyze the degree of match with the dental hygienist's job for each detailed subject. Methods: The 5th edition of 'Dental hygiene and learning objectives' was analyzed by subject based on Bloom's cognitive domain classification from March 10 to April. In addition, the degree of match between the contents of the secondary job analysis of the dental hygienist and the learning objectives for each detailed subject were analyzed. Results: The total number of dental hygiene learning objectives was 2,975 (2,762 theory, 52 practice). Among the cognitive domains, the comprehension domain was the most common (79.8%), and the skill domain was very low (4.9%). In the job for each detailed subject of dental hygiene, the most frequently performed was 'dental prophylaxis and practice' with 103 subjects. Conclusions: Overall, dental hygiene learning objectives are mostly theory-oriented, so it is necessary to expand and improve in the direction related to the jobs that clinical dental hygienists perform in the field. In addition, it is necessary to continuously develop timely learning goals, and prepare active strategies for developing high-quality items.

The development and validation of a prehospital emergency delivery protocol for paramedics (병원 전 응급구조사의 응급분만 프로토콜 개발 및 타당도 검증)

  • Keun-Young Kim;Byung-Soo Kim
    • The Korean Journal of Emergency Medical Services
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    • v.27 no.2
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    • pp.79-89
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    • 2023
  • Purpose: This study aimed to develop a standardized protocol of systematic and explicit roles and procedures for paramedics for the maternal and newborn management during prehospital emergency delivery situations. Methods: This study is a methodological study with detailed step-by-step items proposed by an expert group based on evidence from a literature review. The content validity of the proposed preliminary prehospital emergency delivery protocol was verified before the final protocol was confirmed. Results: All 23 detailed items of the preliminary protocol met the reference cut-off (CVI value of 0.8 or higher). After considering, revising, and supplementing expert opinions for each of the 23 items 23 detailed in the proposed protocols, four steps were confirmed. The steps included five items for the management of the imminent mother, 10 items for delivery progress, five items for newborn management, and three items for postpartum management of the mother. Conclusion: This study's findings may serve as reference for developing future specialized training programs for paramedics, to ensure enhanced competence in prehospital emergency delivery situations. The protocol should be reviewed and revised periodically to ensure currency.

Evaluation of Liquefaction Strength Based on Korean Earthquake Magnitude (국내 발생 지진규모를 고려한 액상화저항강도 산정)

  • 신윤섭;박인준;최재순;김수일
    • Journal of the Korean Geotechnical Society
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    • v.15 no.6
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    • pp.307-317
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    • 1999
  • The purpose of this study is to utilize conventional procedures for evaluation of liquefaction potential and to compare the results obtained by modified detailed method based on Korean earthquake magnitude (M=6.5). Liquefaction potential is assessed by comparing liquefaction strength of soil and cyclic shear stress generated in the soil layers during earthquakes. The cyclic shear stress is computed from the earthquake response analysis, and liquefaction strength of soil is evaluated by using results from cyclic triaxial tests. The cyclic triaxial tests are performed on many different conditions of sample ; relative densities(50%, 60%, and 70%), initial effective confining pressures (70kPa, 100kPa, and 150kPa), and fine contents(10%, 20%, and 30%). From the result of comparing the conventional procedure with the modified detailed method, it is found that the modified detailed method tends to evaluate larger safety factor against liquefaction in the weak sand site$(FS \leq1.5)$. Therefore in this case, it is suggested that liquefaction potential should be evaluated by using the modified detailed method based on cyclic triaxial tests. It is also found that in modified detailed method based on earthquake magnitude 6.5, critical depth where liquefaction can be generated is around 15m from the ground surface.

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Systematic improvement method depending on analysis of inductive contents on university volunteers'satisfaction & dissatisfaction on participating in stipulated PGA pro-golf competitions (대학생 자원봉사자의 PGA 정규 투어 프로골프대회 참여 만족·불만족 귀납적 내용분석에 따른 제도적 개선 방안)

  • Nam, Jae-Jun;Jung, Seong-Un
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.3
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    • pp.526-542
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    • 2020
  • The purpose of this study was to examine contents through inductive analytic method and make systematic improvement method on satisfaction & dissatisfaction of university students participating in stipulated PGA pro-golf competitions as volunteers. The objects of this study were university students participating in 2019 The CJ Cup@Nine Bridges as volunteers. Excluding 76 copies judged to be disloyal out of 300 collected copies, the researcher analyzed the contents of 224 copies. To begin with, the contents were categorized in 10 detailed areas & 5 general areas by implementing the 2nd inductive categoric analysis mainly on the basis of 408 raw materials, the overall detailed areas of satisfaction on participation. Checking specifically, first, "satisfaction on experience in golf competition" was divided into golf competition, golf information, golf course. Second, "satisfaction on player" was divided into direct watching and famous player. Third, "satisfaction on environment" was divided into satisfaction on place & satisfaction on facilities. Fourth, "satisfaction on participation" was divided into satisfaction on service & satisfaction on competence. Finally, "satisfaction on interpersonal relation" was divided with application same to that of detailed area. In the case of dissatisfaction on participation, the contents were categorized in 10 detailed areas & 3 general areas by implementing the 2nd inductive categoric analysis mainly on the basis of 369 raw materials, the overall detailed areas. Checking specifically, first, dissatisfaction on competition operating system was detailedly divided into dissatisfaction on job system, dissatisfaction on operating system, dissatisfaction on delivery system. Second, dissatisfaction on treatment for volunteers was divided into dissatisfaction on treatment for volunteers & dissatisfaction on work hours. Third, dissatisfaction on welfare for volunteers was divided into dissatisfaction on food & beverage, dissatisfaction on costumes and dissatisfaction on incidental facilities.

A Study of the Junior Nursing College Students실 Role during Clinical Practice (간호전문대학생들의 임상실습현장에서의 수행에 관한 연구 -실습의 내용 빈도를 중심으로-)

  • 권경남
    • Journal of Korean Academy of Nursing
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    • v.13 no.3
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    • pp.1-33
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    • 1983
  • The role and function of nursing care nowadays, tend to acquire sophisicated technology because specialization has expended due to increase of the medical population and the improvement of national health standards. To implement nursing care independently as a professional nurse, the apprehension of specific knowledge and skill should be acquired during basic nursing education. So it is important for nursing education not only to include theory and actual techniques, but also to strengthen the practical training in the actual clinical setting. This study was carried out with the following objectives; 1. To survey the detailed content and frequency of actual nursing students display during their clinical training. 2. To investigate the detailed content and frequency of actual nursing behavior which students display in each clinical a area. 3. To identify the motive for selection of nursing as their major and to determine the degree of self confidence, extent of knowledge and recognition of nursing responsibility. 4. To observe the relationship between actual nursing behavior and each of the following; 1) Motive for selecting nursing as a major 2) Self confidence 3) Knowledge of nursing care 4) Recognition of nursing responsibility The conclusions of this study were as follows; 1. Among the detailed nursing behavior which junior nursing college students carry out in clinical training; taking respiration's showed the highest frequency, and taking body temperatures, blood pressures, and pulses and making beds were next in frequency in this order. 2. In detailed nursing behaviors according to clinical area; taking vital signs showed the highest frequency in the emergency room, pediatric ward, orthopedic ward, general surgical ward and internal medicine ward. However, in the operating room, assisting with endotracheal tube insertion and sterile techniques were showen to have the highest frequencies. In nursery, umbilical cord care and the measurement of body weight were the highest in frequency In neurosurgical ward, the measurement of vital signs, changing position and tracheostomy care were the highest in frequency. In obstetric and gynecological ward and in the delivery room, checking duration, intensity and frequency of contractions was the highest in frequency. 3. In regard to the motive for majoring in nursing, the aptitude and interest of the student had the highest percentage(32.86%), and self-confidence in nursing activities (M=3.36), knowledge in nursing activities.(M=3. 09), and the recognition of the nursing activity (M= 3.76) wire in the middle range. 4. When the detailed nursing behaviors were compared with motive, self confidence, knowledge and recognition, it was found that when the nursing behavior was difficult and regarding much endeavor although the motive was high, the frequency of the nursing behavior was rather low. But in the cases in which there was much self confidence and a high level of skill was required, nursing behavior was carried more frequently. When there was muck self confidence and skill was not required, the frequency of nursing behavior was rather low. In the cases of a high level of knowledge, the frequency of nursing behavior was low and when recognition for nursing behavior was given the frequency of nursing behavior was low.

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