• 한국환경보건학회지
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• 제48권5호
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• pp.282-289
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• 2022

Background: Airborne fungi are ubiquitous in the air and exposure to an airborne fungus can be a significant risk factor. The composition of fungi has been potentially important for human health, especially for respiratory diseases like asthma and atopic dermatitis. Therefore, we attempted to ascertain what kind of airborne fungi affect human health at a nationwide level. Objectives: This study was carried out to provide information on indoor fungi distribution at multi-use facilities throughout South Korea. Methods: We classified our data by region and public facility after collection, cultivation, and identification via the sequencing of the ITS (internal transcribed spacer) region. We investigated whether or not the proliferation of HaCaT cells was affected by the identified airborne fungi. Results: In our data, the most isolated airborne fungi by region were Penicillium spp (Seoul, Daegu), Periconia sp (Gyeonggi-do), Iprex sp (Gangwon-do), Phanerochaete sp (Busan), Bjerkandera sp (Gwangju), and Aspergillus sp (Jeju-do). In the public facilities, the most detected fungi were Cladosporium sp (public transport), Penicillium sp (apartment house, retail market, financial institution, karaoke room), Bjerokandera sp (underground parking lot, public toilet, medical institution), Periconia sp (retail store), and Fusarium sp (general restaurant). Next, we selected twenty airborne fungi to examine their cytotoxicity and proliferation of human skin cells. In this experiment, the proliferation of the cells was influenced by most of the identified fungi. In case of the cytotoxicity test, most genera except for Rhodotorula sp and Moesziomyces sp showed cytotoxicity in HaCaT cells. Conclusions: The distribution of mold in the indoor air in multi-use facilities in South Korea differs from region to region, and this is an indicator that should be considered in future health impact studies. In addition, as a result of culturing about 20 types of bacteria dominant in indoor air, it was found that most (90%) inhibit the growth of skin cells, which can be harmful to health. An in-depth study of the health effects of floating fungi is needed.

• 한방안이비인후피부과학회지
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• 제36권1호
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• pp.96-109
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• 2023

Objectives : The objective of this study is to predict how future infectious diseases will affect healthcare utilization patterns within korean medicine hospitals and, specifically, departments of ophthalmology, otolaryngology and dermatology of Korean medicine. Methods : We retrospectively analyzed medical records of patients who visited Kyung Hee University Korean Medicine Hospital during Pre COVID-19(from March 1st, 2018 to Feb 29th, 2019) and Post COVID-19(from March 1st, 2019 to Feb 28th, 2022). The data was analyzed by visit type and diagnosed disease code. Results : 1. After the COVID-19 break-out, the number of first visit patients increased but those of revisit patients, total patients, and revisit/first visit ratio decreased in all departments. 2. In Kyung Hee University Korean Medicine Hospital, the decrease rate of the number of total patients diagnosed with diseases of respiratory system(J00-J99) was the highest, while that for diseases of circulatory system(I00-I99) was the lowest. 3. In the department of Ophthalmology and Otolaryngology of Korean Medicine, the decrease rate of the number of total patients with nose disease was the highest, followed by eye disease and ear disease. 4. In the department of Dermatology of Korean Medicine, the number of total patients with dermatitis remained consistent, while the number of total patients with skin appendage disease showed relatively high decrease rate. Conclusions : Severe or chronic diseases showed relatively low decrease rate of the number of total patients. While mild diseases or diseases with high probability of exposure to COVID-19 infection during treatment showed high decrease rate of the number of total patients.

• 문화기술의 융합
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• 제8권6호
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• pp.849-854
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• 2022

본 연구에서는 참당귀(Angelica gigas Nakai)의 핵심 유효 성분인 decursin과 decursinol angelate를 높은 수율로 추출한 초임계 이산화탄소 선택 추출물의 항염증 효능을 에탄올 추출물과 비교하여 측정하였다. 항염증 효능을 측정하기 위해 염증 매개 인자인 nitric oxide(NO), 염증성 사이토카인인 interleukin(IL)-6, IL-8 생성량을 측정하였다. NO 생성량은 lipopolysaccharide(LPS)로 염증 반응을 유도한 Raw 264.7 세포를 대상으로 Griess assay를 통해 측정하였고, IL-6 및 IL-8 생성량은 tumor necrosis factor(TNF)-α로 염증 반응을 유도한 human keratinocyte cell line(HaCaT) 세포를 대상으로 enzyme-linked immunoadsorbent assay(ELISA)를 통해 측정하였다. NO 생성량은 초임계 이산화탄소 추출물이 에탄올 추출물에 비해 뛰어나게 억제하였다. IL-6 및 IL-8 생성량 또한 에탄올 추출물은 오히려 증가시키는 반면, 초임계 이산화탄소 추출물은 6.25 ㎍/mL의 농도에서 통계적으로 유의하게 억제하였다(P<0.01). 우리는 이러한 결과를 통해 참당귀 초임계 이산화탄소 선택 추출물이 아토피 피부염을 완화시키는 항염증 코스메슈티컬 소재로써 활용 가능함을 확인하였다.

• Journal of Plant Biotechnology
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• 제49권4호
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• pp.339-346
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• 2022

본 연구는 벌나무 에탄올 추출물의 황색포도상구균에 대한 항균 및 항산화 활성을 검증하였다. 총 폴리페놀 및 플라보노이드 함량, DPPH와 ABTS 라디칼 소거효능과 FRAP법을 사용한 환원력을 측정하여 벌나무 에탄올 추출물의 항산화활성을 검증하였고, Paper disc법, MIC 및 MBC법을 통하여 황색포도상구균에 대한 벌나무 에탄올 추출물의 항균 활성을 검증하였다. 총 폴리페놀 및 플라보노이드 함량은 각각 266.66 ㎍ GAE/mg와 6.46 ㎍ QE/mg이었다. DPPH와 ABTS 라디칼 소거능은 농도 의존적 소거능을 보였고, 벌나무 에탄올 추출물의 RC₅₀ 값은 각각 21.49, 12.81 ㎍/mL이었다. 환원력을 평가하기 위해 수행된 FRAP 분석에서 벌나무 추출물은 0.73 ± 0.19 mM FeSO₄ E/mg의 효능을 보여주었다. Paper disc법을 이용한 황색포도상구균에 대한 벌나무 추출물의 항균 활성은 2 mg에서 3 mm의 저해환을 보여주었으며, 최소 저해농도 연구에서는 1 mg/mL 이상의 농도에서 80% 이상의 억제력을 보여주었고, 16 mg/mL 이상의 농도에서 최소저해 농도를 확인할 수 있었다. 또한, 최소살균농도 연구에서는 32 mg/mL에서 최소살균농도를 확인할 수 있었다. 벌나무 추출물을 사용한 항산화 및 황색포도상구균에 대한 항균 활성을 확인한 결과 벌나무 추출물의 아토피피부염에 유효한 물질로서의 가능성을 확인할 수 있었다.

• 한방안이비인후피부과학회지
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• 제36권2호
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• pp.60-75
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• 2023

Objectives : A bibliometric approach using network analysis was applied to explore the global trends of research on herbal medicine for atopic treatment. Methods : Articles related to herbal medicine on atopic treatment from 2003 to 2022 were retrieved from Web of Science Core Collection. Extracted records were analyzed according to the publication year, research area, journal title, country, organization, author and keyword. The VOSviewer program was used to visualize the trends and the research hotspots in herbal medicine for atopy. Results : Analysis of 406 articles indicated the consistent increase of using herbal medicine for atopic treatment over the last 20 years. The most productive country and research organization in issuing articles were South Korea and Kyunghee university. Many articles have been published in research areas such as 'integrative complementary medicine' and 'pharmacology pharmacy'. By evaluating the total link strength, the average publication year and the average citations of countries and authors, the influential countries and authors were identified. A network analysis based on the co-occurrence and the publication year of keywords revealed the relevant characteristics and trends of herbal medicine for atopy. The most up-to-date keywords were 'topical application', 'skin barrier' and 'care'. Conclusions : This bibliometric study examined the overall trends and the time-based development of herbal medicine for atopic treatment. The current study would be useful not only for grasping the global network hub of research on herbal medicine for atopic treatment, but also to explore the new directions for future research.

• 한방안이비인후피부과학회지
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• 제36권3호
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• pp.1-11
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• 2023

Objectives : The objective of this study is to identify the characteristics of outpatients and the frequently diagnosed diseases in dermatology of Korean medicine through periodic research and comparison with previous studies. Methods : We analyzed medical records of new outpatients who visited department of dermatology at Kyung Hee university Korean medicine hospital between January 1, 2018 and December 31, 2022. We analyzed the patient demographic characteristics and the frequency of diagnosed diseases. Then we compared these results with previous studies in 2000, 2007 and 2016. Results : 1. Average number of patients and proportion of male patients have increased from 1996 to 2022. 2. From 1996 to 2022, there was a decrease in the proportion of patients aged 0-39 and an increase in the proportion of patients aged over 40. 3. The distribution proportions of eczema, atopic dermatitis, and rosacea during 2018-2022 have increased compared to those during 2011-2015. 4. The distribution of diseases according to age appears to vary based on the epidemiological characteristics of the diseases. 5. The distribution of diseases according to gender also appears to vary based on the epidemiological characteristics of the diseases, as well as cosmetic issues related to the affected areas. Conclusions : Considering the sustained trend of a high proportion of non-infectious inflammatory diseases since 1996, it is important to enhance treatment capacity through continuous research.

• 대한화장품학회지
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• 제49권3호
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• pp.277-283
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• 2023

물푸레나무(Fraxinus rhynchophylla Hance, F. rhynchophylla)는 동아시아에서 널리 사용되어 온 전통적인 약용 식물로, 만성 기관지염, 세균성 이질과 시력 개선 등을 위해 사용돼 왔다. 물푸레나무에는 esculin, esculetin, fraxin 및 fraxetin과 같은 다양한 형태의 쿠마린이 존재하며, esculetin은 항산화, 항염, 항균, 항암 및 아토피성 피부 염증 완화 등의 효과를 가지고 있다. 지금까지 물푸레나무 내 유효 성분을 증가시키는 공정개발에 관한 연구는 보고되지 않았다. 따라서 본 연구의 목적은 물푸레나무 내 esculetin함량을 증가시키고, 항염 및 항산화 효능을 증대시키기 위한 물푸레나무 생물전환 공정을 개발하는 것이다. 당분해효소 viscozyme L을 이용하여 물푸레나무에 처리한 결과 물푸레나무 효소처리물(FRE-VL)은 물푸레나무 추출물(FRE)대비 esculetin 함량이 3.1배 증가하였으며, 항염 활성 증대와 DPPH 라디칼 소거 활성의 효과를 나타내었다. 본 연구를 통해 화장품 분야에서 생물전환 기술을 적용한 물푸레나무 효소처리물의 신규 화장품 기능성 소재로의 활용 가능성을 확인하였다.

• 한국위험물학회지
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• 제2권1호
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• pp.18-26
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• 2014

We analyzed the demand of competent authorities requiring adequate technical information for initial investigation of chemical accidents. Reflecting technical reports on chemical accident response by environmental agencies in the U.S. and Canada, we presented information on environmental diffusion and toxic effects available for the first chemical accident response. Hydrogen fluoride may have the risk potential to corrode metals and cause serious burns and eye damages. In case of inhalation or intake, it could have severe health effects. The substance itself is inflammable, but once heated, it decomposes producing corrosive and toxic fume. In case of contact with water, it can produce toxic, corrosive, flammable or explosive gases and its solution, a strong acid, may react fiercely with a base. In case of hydrogen fluoride leak, the preventive measures are to decrease steam generation in exposed sites, prevent the transfer of vapor cloud and promptly respond using inflammable substances including calcium carbonate, sodium bicarbonate, ground limestone, dried soil, dry sand, vermiculite, fly ash and powder cement. The method for fire fighting is to suppress fire with manless hose stanchions or monitor nozzles by wearing the whole body protective clothing equipped with over-pressure self-contained breathing apparatus from distance. In case of transport accident accompanied with fire, evacuation distance is 1,600m radius. In cae of fire, fire suppression needs to be performed using dry chemicals, CO₂, water spray, water fog, and alcohol-resistance foam, etc. The major symptoms by exposure route are dyspnoea, bronchitis, chemical pneumonia and pulmonary edema for respiration, skin laceration, dermatitis, burn, frostbite and erythema for eyes, and nausea, diarrhea, stomachache, and tissue destruction for digestive organs. In atmosphere, its persistency is low, and its bioaccumulation in aquatic organism is also low.

• 생명과학회지
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• 제34권3호
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• pp.170-178
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• 2024

지방줄기세포는 다양한 세포로 분화할 수 있어서 세포치료제로 주목받고 있으나 세포 증식 속도가 느리고 세포의 수명이 짧아 임상 적용에 어려움이 있다. 본 연구팀은 선행연구에서 지방줄기세포의 증식속도를 향상시키고 세포 수명을 연장시키기 위해 SV40의 T항원 유전자를 사람의 ADSC에 도입하여 불사화시킨 ADSC 세포주를 제작하였으며 ADSC-T라고 명명하였다. 본 연구에서는 ADSC-T 세포의 분화능력을 검증하고, ADSC-T 세포를 배양하여 획득한 무혈청 배양액의 항염증 효능을 평가하였다. 그 결과, ADSC-T 세포주는 지방세포와 골세포로 분화할 수 있는 능력을 보유하고 있어 줄기세포의 가장 중요한 특징인 분화능력을 유지하고 있음을 알 수 있었다. 또한 ADSC-T의 배양액은 염증을 유발하는 NF-κB의 활성을 억제하였고, NF-κB의 표적 유전자인 COX-2와 iNOS의 발현도 억제하였다. 또한 ADSC-T 배양액은 ERK, JNK, p38의 인산화를 저해하여 세포 내 염증 유발 신호 전달 회로인 MAPKs 회로를 억제하는 것으로 나타났으며, 염증을 유발하는 cytokine인 TGF-β, TNF-α, IL-6, IL-13의 발현도 억제하는 효과를 보여 강한 항염증 효능이 있을 것으로 추정되었다. 실제로, 아토피 모델 쥐인 Nc/Nga 마우스를 DNCB로 처리하여 아토피 피부염을 유발한 뒤 ADSC-T 배양액을 도포한 결과 강한 치료 효과를 나타내었다. 이상의 결과로부터, 불사화된 ADSC-T 세포주는 지방줄기세포의 장점과 효능을 유지하고 있으며 향후 지방줄기세포의 세포 수급 문제를 해결할 수 있을 뿐 아니라 임상 적용이 가능한 배양액의 생산에도 널리 이용될 수 있을 것으로 사료된다.

• 대한예방한의학회지
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• 제19권1호
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• pp.95-107
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• 2015

Objective : To use and its affecting factors of patients' outpatient treatment that have single and multiple chronic illnesses Method : We used the 2011 study by the Ministry of Health and Welfare, "Report on Usage and Consumption of Korean Medicine." This report was conducted on outpatients and inpatients that visited Korean traditional medicine, from August 25th, 2011, to September 30th, 2011. Our research was based on 1729 patients with chronic diseases aged 30 and over who received outpatient treatment during the last three months. Results : There were 1365 patients with non-complex chronic diseases, while there were 364 patients with complex chronis diseases. Patients had 1 - 8 chronic diseases, and the average number (standard deviation) was 1.26 (0.59). There were statistically significant differences by sex(P<0.0001), age(p=0.0045), marriage (p=0.0060), education level(p<0.0001), income level(P=0.0063), and types of health insurance(p=0.0023). The diseases most common among patients with non-complex chronic diseases were: low back pain, arthritis, gastrointestinal disorder, frozen shoulder, side effects from motor accidents, high blood pressure, fracture, stroke, diabetes, cancer, atopic dermatitis, and asthma. The diseases most common among patients with non-complex chronic diseases were: arthritis+low back pain, low back pain+gastrointestinal disorder, low back pain+side effects from motor accidents, low back pain+frozen shoulder, arthritis+gastrointestinal disease, gastrointestinal disease+frozen shoulder, arthritis+low back pain+gastrointestinal disease, high blood pressure+arthritis, arthritis+low back pain+frozen shoulder, arthritis+fracture, and arthritis+side effects from motor accidents. There were statistically significant differences among the usage of medical clinics by: frequently used clinic (p<0.0001), number of treatment (p<0.0001), the cost of outpatient treatment (p=0.0073), the satisfaction rate (p=0.0171), whether the clinic is the preferred clinic (p=0.0040). In model 1, men than women, people who had local benefits instead of type 1 medical aid, and patients with complex chronic diseases were more likely to use Korean medical clinics. In model 2, men than women, people who had local benefits than people with types 1 and 2 medical aids, people who went to pharmacies and Korean medicine pharmacies than people who went to hospitals, people who went to get treatment 1-10 times than people who visited 11-20 times and more than 41 times, and people who spent less than ten thousand Korean won than people who spent 1 to 2 million Korean won, and people without complex chronic diseases were more likely to use Korean medical treatment. Conclusion : There were differences in sociodemographic characteristics and the usage of medical clinics between patients with non-complex chronic diseases and patients with complex chronic diseases. Among patients with complex chronic diseases, women, patients with types 1 and 2 medical aid, patients who used Korean medical clinics, patients who were treated 11-20 times and more than 41 times, and patients who spent 1 million to 2 million Korean won on outpatient treatment used less treatment than patients with non-complex chronic diseases. However, patients with complex chronic diseases used pharmacies and Korean medicine pharmacies more.