• 한국융합학회논문지
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• 제13권4호
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• pp.653-657
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• 2022

구강 질환은 전 세계적으로 심각한 건강 및 경제적 부담을 야기하여 사람들의 삶의 질을 크게 저하시킨다. 대표 구강질환인 치아우식증은 S. mutans에 의해 발생한다. 또한, 구강 병원성 미생물에는 면역 반응이 일어나면서 다양한 구강 질환을 유발할 수 있는 지질다당류(lipopolysaccharide, LPS)가 함유되어 있다. 본 연구의 목적은 구강 질환을 조절하기 위해서 대나무 잎 추출물(BLE)의 항산화 및 항균 효과를 조사하는 것이다. THP-1, oral fibroblasts, S mutans 배양액에 댓잎 추출물을 0-8% 농도별로 처리하여 실험을 진행하였다. 그 결과 단핵세포주와 구강섬유아세포에서 BLE 농도에 따른 항산화 효과를 확인하였다. 또한, BLE 농도에 따른 S. mutans의 항균효과를 입증하였다. 따라서 BLE가 구강질환의 예방 또는 치료에 사용될 수 있음을 시사한다.

• The Journal of Advanced Prosthodontics
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• 제16권3호
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• pp.151-162
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• 2024

PURPOSE. This study aimed to analyze factors influencing the success and failure of implant prostheses and to estimate the lifespan of prostheses using standardized evaluation criteria. An online survey platform was utilized to efficiently gather large samples from multiple institutions. MATERIALS AND METHODS. During the one-year period, patients visiting 16 institutions were assessed using standardized evaluation criteria (KAP criteria). Data from these institutions were collected through an online platform, and various statistical analyses were conducted. Risk factors were assessed using both the Cox proportional hazard model and Cox regression analysis. Survival analysis was conducted using Kaplan-Meier analysis and nomogram, and lifespan prediction was performed using principal component analysis. RESULTS. The number of patients involved in this study was 485, with a total of 841 prostheses evaluated. The median survival was estimated to be 16 years with a 95% confidence interval. Factors found to be significantly associated with implant prosthesis failure, characterized by higher hazard ratios, included the 'type of clinic', 'type of antagonist', and 'plaque index'. The lifespan of implant prostheses that did not fail was estimated to exceed the projected lifespan by approximately 1.34 years. CONCLUSION. To ensure the success of implant prostheses, maintaining good oral hygiene is crucial. The estimated lifespan of implant prostheses is often underestimated by approximately 1.34 years. Furthermore, standardized form, online platform, and visualization tool, such as nomogram, can be effectively utilized in future follow-up studies.

• International Journal of Oral Biology
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• 제35권2호
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• pp.69-74
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• 2010

The mu opioid receptor (MOR) has been regarded as the main site of interaction with analgesics in major clinical use, particularly morphine. The repressor element-1 silencing transcription factor (REST) functions as a transcriptional repressor of neuronal genes in non-neuronal cells. However, it is expressed in certain mature neurons, suggesting that it may have complex and novel roles. In addition, the interactions between MOR and REST and their functions remain unclear. In this study, we examined the effects of morphine on the expression of REST mRNA and protein in human neuroblastoma NMB cells to investigate the roles of REST induced by MOR activation in neuronal cells. To determine the effects of morphine on REST expression, we performed RT-PCR, real-time quantitative RT-PCR, western blot analysis and radioligand binding assays in NMB cells. By RTPCR and real-time quantitative RT-PCR, the expression of REST was found to be unchanged by either the MOR agonist morphine or the MOR specific antagonist CTOP. By western blot, morphine was shown to significantly inhibit the expression of REST, but this suppression was completely blocked by treatment with CTOP. In the radioligand binding assay, the overexpression of REST led to an increased opioid ligand binding activity of endogenous MOR in the NMB cells. These results together suggest that morphine inhibits the expression of REST in human neuroblastoma cells through a post-transcriptional regulatory mechanism mediated through MOR.

• 대한수의학회지
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• 제50권3호
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• pp.165-169
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• 2010

Recently, the number of stray dogs is proportionate to the increase of the number of the companion dogs. Sarcoptic scabiei var canis, causing scabies, is one of the most important canine zoonotic arthropods in Korea and around the world. Thus, we have tried to know the prevalence of canine scabies in the stray dogs in Korea. A total of 565 stray dogs were collected from the rescue centers all over the country from Jan. 2006 to Dec. 2006. They were grouped with euthanasia or natural death and examined for the gender and age estimated by dental formula. To identify the lesions, the whole body was grossly examined and tested pathologically. Thirty two (5.66%) of 565 dogs were diagnosed as canine scabies. Dogs from urban areas had fewer scabies (0.62%) than those of rural areas (12.5%). Prevalence of scabies in male and female dogs was no difference as 5.96% and 5.25%. Euthanasia group showed higher prevalence (6.48%) than natural death group (2.44%) in scabies. Old dogs over five years showed lower infestration (1.82%) in scabies. In histopathological findings, there were mites in the burrows formed in the subcorneal space. Acanthosis, hyperkeratosis with crust, and vascular dilatation were main findings. One hundred thirteen (20%) of 565 stray dogs were diagnosed to have skin disease. Among them, canine scabies is the most prominent ectoparasite as 5.66 %. With previous reports on human infection in Korea, canine scabies must be regarded as the important zoonotic canine skin disease. Accordingly, for the human and canine hygiene it is imperative that stray dogs with skin problems are segregated and tested for the parasites to treat properly as soon as arriving at rescue shelter.

• International Journal of Oral Biology
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• 제41권3호
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• pp.105-111
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• 2016

${\beta}-carotene$ is present in carrots, pumpkins, and sweet potatoes. It suppresses many types of cancers by regulating cellular proliferation and apoptosis through a variety of mechanisms. However, the effects of ${\beta}-carotene$ on oral cancer cells have not been clearly established. The main goal of this study was to investigate the effects of ${\beta}-carotene$ on cell growth and apoptosis in oral cancer cells. Our results demonstrate that treatment with ${\beta}-carotene$ induced inhibition of cell growth, and that the effect was dependent on ${\beta}-carotene$ treatment time and concentration in KB cells. Furthermore, treatment with ${\beta}-carotene$ induced nuclear condensation and fragmentation in KB cells. ${\beta}-carotene$ promoted proteolytic cleavage of procaspase-3, -7, -8 and -9 with associated increases in the concentration of cleaved caspase-3, -7, -8 and -9. In addition, the level of cleaved PARP was increased by ${\beta}-carotene$ treatment in KB cells. These results suggest that ${\beta}-carotene$ can suppress cell growth and induce apoptosis in KB human oral cancer cells, and that it may have potential usefulness in anti-cancer drug discovery efforts.

• 대한본초학회지
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• 제31권5호
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• pp.7-13
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• 2016

Objectives : Camellia sinensis (Theaceae) possesses a various beneficial effects such as free radical-scavenging, inactivation of urokinase in cancer cell proliferation, antibacterial, and hypotensive. Dental caries is one of the most common oral infectious disease in a human. Oral microorganisms play a significant role in the etiology of dental caries. An aberration to this ecology due to dietary habits, improper oral hygiene or systemic factors lead to an increase in cariogenic microorganisms. Cariogenic microorganisms like Streptococcus mutans and Streptococcus sobrinus encourage the accumulation and adherence of plaque biofilm by metabolizing sucrose into glucans. The purpose of this study was to investigate the antimicrobial activity of phenolic compounds of Camellia sinensis and R-carvone, monoterpenes, is can be found naturally in numerous essential oils, on Streptococcus mutans and Streptococcus sobrinus .Methods : The antimicrobial activity of these compounds was determined by the broth microdilution method and checkerboard dilution assay to investigate the potential synergistic effects of each five compounds of Camellia sinensis (C. sinensis) and R-carvone.Results : C. sinensis-isolated compounds and R-carvone were determined with MIC of more than 1,000 ㎍/mL. However, the combination test showed significant synergism against S. mutans and S. sobrinus, implicated in the lowered MICs.Conclusions : These results suggest that combinatory application of phenolic five compounds (theophyllin, l-theanine, epicatechin, epicatechin gallate, and caffeine) from C. sinensis and R-carvone has a potential synergistic effect and thus may be useful as a mouthrinse in helping control cariogenic microorganism.

• 한국융합학회논문지
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• 제12권4호
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• pp.95-103
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• 2021

본 연구의 목적은 연령대에 따른 생애주기별 감염병에 대한 지식, 태도, 예방 및 관리에 대한 교육요구도를 파악하여 교육프로그램 개발을 위한 기초자료의 제공이다. 2021년 2월 한 달 동안 한국갤럽의 온라인·모바일 조사를 통해 만 19세 이상 성인 328명을 조사하였다. 연구내용은 일반적 특성, 감염 관련 개인위생실천, 감염 관련 지각된 위험성, 감염병에 대한 중요도 및 지식정도, 감염병 예방 및 관리에 대한 교육요구도이다. 조사 자료는 PASW Statistics ver 20.0 통계 프로그램을 이용하였다. Borich 요구도 공식으로 환산하여 중요도와 지식정도를 합산하여 순위를 분석한 결과 1순위(Borich 3.11)는 감염병의 치료 방법, 2순위(Borich 2.15)는 감염병 의심 및 확진 시 처리절차, 3순위(Borich 1.75)는 감염병의 전파경로, 4순위(Borich 1.73)는 감염병의 예방 방법, 5순위(Borich 1.50)는 감염병의 진단 및 검사방법, 6순위(Borich 1.45)는 감염병의 특성, 7순위(Borich1.38)는 감염병의 주요 증상 등으로 조사되었다. 본 연구 결과는 감염병 예방 및 관리에 대한 교육요구도를 반영한 교육프로그램 개발을 통해 대상자의 신체건강, 정신건강 및 심리적 안녕감을 증가시키는 데 기여할 수 있을 것이다.

• 대한소아치과학회지
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• 제47권3호
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• pp.327-336
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• 2020

이 연구의 목적은 치아우식증 고위험군에 영향을 미치는 인구사회학적 특성과 구강건강관련행태 요소들을 파악하는 것이었다. 2015년도에 실시한 아동구강건강실태조사 자료 중 12세 아동 23,089명의 구강상태 및 설문조사 자료를 분석에 이용하였다. 대상자를 Significant caries (SiC) index에 따라 고위험군과 저위험군을 분류하고 각 변수들의 영향력을 분석하였다. 연구 결과, 한국 12세 아동의 치아우식증 고위험군의 SiC 지수는 5.08로 저위험군에 비해 약 9.6배 높게 나타났다. 고위험군과 관련된 위험요인은 제1대구치 치면열구전색 여부, 지난 1년간 치과 수진 여부, 주관적 구강건강인식, 성별, 지역, 1일 평균 간식섭취 횟수, 구강보조용품의 사용, 점심식사 후 칫솔질 여부의 순으로 나타났다. 치아우식증 고위험군의 위험요인으로 나타난 항목을 참조하여 적절한 예방조치와 구강보건교육이 시행된다면 아동들의 구강건강 향상에 도움이 될 것으로 사료된다.

• 대한치과의사협회지
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• 제53권1호
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• pp.36-46
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• 2015

Objectives: The objective of this work was to investigate the hospital nursing care of oral and maxillofacial health in jeju province. Methods: 438 Registered nurses(RN) who were working at each of secondary hospitals in Jeju province had responded to the questionnaire. The data were analyzed via frequency analyses and one-way ANOVA to assess the state of RN on hospital nursing care of oral and maxillofacial health. Results: The class of education on density in formal density that marked '0 hour' and '1-3 hours' were 73.5% and 19.9%, respectively. The class of refresher training on density that marked '0 hours' and '1-3 hours' were 92.9% and 6.6%, aggregately 99.5%. The nursing education on appearance after tumor of maxillofacial area that marked 'formal education' and 'none' were 45.2% and 52.1%, respectively. The score of question 'function, effect and side effect of hexamedin gaggle' was $2.68{\pm}0.95$ by Likert 5-point scale. Likewise, the scores were $2.82{\pm}0.88$ on question 'management of removal denture', $2.83{\pm}0.95$ on question 'preventive dental treatment before anticancer therapy', $2.88{\pm}0.86$ on question 'function of saliva', $2.96{\pm}0.99$ on question 'oral management of tube feeding patient', $3.13{\pm}1.00$ on question 'bacterial endocarditis from oral microflora', $3.36{\pm}0.89$ on question 'dysphagia' and $3.62{\pm}1.03$ on question 'aspiration pneumonia'. RN replied that 'lack of knowledge' and 'delay of cooperation' formed 53.7% and 33.3% respectively, on question 'problem in dental consultation other diseased patient'. Conclusions: From this study, it is necessary for RN and student of nursing science to be educated on the oral and maxillofacial nursing. Authors suggest further co-study and nation-wide research.

• Restorative Dentistry and Endodontics
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• 제35권2호
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• pp.116-124
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• 2010

본 연구에서는 복합 레진을 이용한 2급/5급 와동의 직접수복에 있어서 치과의사의 성별에 따른 술식의 차이를 비교하였다. 2008년 대한치과의사협회에 등록된 치과의사 12,193명을 대상으로 이 메일을 통한 설문조사를 실시하였다. 이 메일 수신이 확인된 2,632명 중 840명이 응답하였으며 응답자의 남녀 비율은(남 78.9%, 여 21.1%) 전체 치과의사의 남녀 비율과 유의한 차이를 보이지 않았다(p > 0.05). Chi-square test 와 multiple logistic regression analysis 를 이용하여 남녀간 술식의 차 이를 검증하였다. 2급 와동 수복에서 여자치과의사는 4회 이상의 적층 분할 수복을 하는 경향이 남자치과의사에 비해 1.87배 높았으며, 술식 당 30분 이상 소요하는 경향은 2.72배 높았다(p < 0.05). 5급 와동 수복에서 여자치과의사는 베이스를 사용하는 경향이 1.83배 높았으며, 술식 당 20분 이상 소요하는 경향은 1.63배 높았다(p < 0.05). 본 설문조사에 따르면 남녀 성별에 따라 복합 레진 수복 술식의 차이가 존재하는 것으로 나타났다.