Browse > Article
http://dx.doi.org/10.6116/kjh.2016.31.5.7.

Antimicrobial synergism of Camellia sinensis-isolated five phenol compounds and R-(-)-carvone against mutans streptococci  

Song, Ok-Hee (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Kang, Ok-Hua (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Mun, Su-Hyun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Kim, Min-Chul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Han, Young-Sun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Choi, Sung-Hoon (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Publication Information
The Korea Journal of Herbology / v.31, no.5, 2016 , pp. 7-13 More about this Journal
Abstract
Objectives : Camellia sinensis (Theaceae) possesses a various beneficial effects such as free radical-scavenging, inactivation of urokinase in cancer cell proliferation, antibacterial, and hypotensive. Dental caries is one of the most common oral infectious disease in a human. Oral microorganisms play a significant role in the etiology of dental caries. An aberration to this ecology due to dietary habits, improper oral hygiene or systemic factors lead to an increase in cariogenic microorganisms. Cariogenic microorganisms like Streptococcus mutans and Streptococcus sobrinus encourage the accumulation and adherence of plaque biofilm by metabolizing sucrose into glucans. The purpose of this study was to investigate the antimicrobial activity of phenolic compounds of Camellia sinensis and R-carvone, monoterpenes, is can be found naturally in numerous essential oils, on Streptococcus mutans and Streptococcus sobrinus .Methods : The antimicrobial activity of these compounds was determined by the broth microdilution method and checkerboard dilution assay to investigate the potential synergistic effects of each five compounds of Camellia sinensis (C. sinensis) and R-carvone.Results : C. sinensis-isolated compounds and R-carvone were determined with MIC of more than 1,000 ㎍/mL. However, the combination test showed significant synergism against S. mutans and S. sobrinus, implicated in the lowered MICs.Conclusions : These results suggest that combinatory application of phenolic five compounds (theophyllin, l-theanine, epicatechin, epicatechin gallate, and caffeine) from C. sinensis and R-carvone has a potential synergistic effect and thus may be useful as a mouthrinse in helping control cariogenic microorganism.
Keywords
Camellia sinensis; R-carvone; theophyllin; l-theanine; epicatechin; epicatechin gallate; caffeine;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bertolini PF, Biondi Filho O, Pomilio A, Pinheiro SL, Carvalho MS. Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study. J Appl Oral Sci. 2012 ; 20 : 32-7.   DOI
2 Kim JE, Kim HJ, Pandit S, Chang KW, Jeon JG. Inhibitory effect of a bioactivity-guided fraction from Rheum undulatum on the acid production of Streptococcus mutans biofilms at sub-MIC levels. Fitoterapia. 2011 ; 82 : 352-6.   DOI
3 Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard 7th ed. 2006; Document M7-A7. Clinical and Laboratory Standards Institute, Villanova, PA.
4 Mun SH, Joung DK, Kim YS, Kang OH, Kim SB, Seo YS, Kim YC, Lee DS, Shin DW, Kweon KT, Kwon DY. Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine. 2013 ; 20 : 714-8.   DOI
5 Mun SH, Kang OH, Joung DK, Kim SB, Seo YS, Choi JG, Lee YS, Cha SW, Ahn YS, Han SH, Kwon DY. Combination Therapy of Sophoraflavanone B against MRSA: In Vitro Synergy Testing. Evid Based Complement Alternat Med. 2013 ; 2013 : 823794.   DOI
6 Shin JS and Chung HS. Antibacterial Activities of Phenolic Components from Camellia sinensis L. on Pathogenic Microorganisms. Journal of Food Science and Nutrition. 2007 ; 135-40.
7 Shahbazi Y. Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria. J Pathog. 2015 ; 2015 : 916305.
8 Tadesse A, Hymete A, Bekhit AA, Mohammed SF. Quantification of total polyphenols, catechin, caffeine, L-theanine, determination of antioxidant activity and effect on antileishmanial drugs of ethiopian tea leaves extracts. Pharmacognosy Res. 2015 ; 7 : S7-S14.
9 Conde VR, Alves MG, Oliveira PF, Silva BM. Tea (Camellia Sinensis (L.)): a Putative Anticancer Agent in Bladder Carcinoma? Anticancer Agents Med Chem. 2014
10 Anita P, Sivasamy S, Madan Kumar PD, Balan IN, Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm. 2014 ; 6 : 35-9.   DOI
11 Aydın E, Türkez H, Keleş MS. Potential anticancer activity of carvone in N2a neuroblastoma cell line. Toxicol Ind Health. 2015 ; 31 : 764-72.   DOI
12 Maciążek-Jurczyk M, Sułkowska A, Bojko B, Równicka-Zubik J, Szkudlarek-Haśnik A, Zubik-Skupień I, Góra A, Dubas M, Korzonek-Szlacheta I, Wielkoszyński T, Zurawiński W, Sosada K. The influence of fatty acids on theophylline binding to human serum albumin. Comparative fluorescence study. Spectrochim Acta A Mol Biomol Spectrosc. 2012 ; 89 : 270-5.   DOI
13 Abdou AM, Higashiguchi S, Horie K, Kim M, Hatta H, Yokogoshi H. Relaxation and immunity enhancement effects of gamma-aminobutyric acid (GABA) administration in humans. Biofactors. 2006 ; 26 : 201-8.   DOI
14 Kerio LC, Wachira FN, Wanyoko JK, Rotich MK. Total polyphenols, catechin profiles and antioxidant activity of tea products from purple leaf coloured tea cultivars. Food Chem. 2013 ; 136 : 1405-13.   DOI
15 Hajiaghaalipour F, Kanthimathi MS, Sanusi J, Rajarajeswaran J. White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage. Food Chem. 2015 ; 169 : 401-10.   DOI
16 Soares MB, Izaguirry AP, Vargas LM, Mendez AS, Spiazzi CC, Santos FW. Catechins are not major components responsible for the beneficial effect of Camellia sinensis on the ovarian δ-ALA-D activity inhibited by cadmium. Food Chem Toxicol. 2013 ; 55 : 463-9.   DOI
17 Choby GW, Lee S. Pharmacotherapy for the treatment of asthma: current treatment options and future directions. Int Forum Allergy Rhinol. 2015 ; 5 : S35-40.   DOI
18 Jówko E. Green Tea Catechins and Sport Performance. In: Lamprecht M, editor. Antioxidants in Sport Nutrition. Boca Raton (FL): CRC Press/Taylor & Francis; 2015. Chapter 8.
19 Tadesse A, Hymete A, Bekhit AA, Mohammed SF. Quantification of total polyphenols, catechin, caffeine, L-theanine, determination of antioxidant activity and effect on antileishmanial drugs of ethiopian tea leaves extracts. Pharmacognosy Res. 2015 ; 7 : S7-S14.
20 Snoussi M, Noumi E, Trabelsi N, Flamini G, Papetti A, De Feo V. Mentha spicata Essential Oil: Chemical Composition, Antioxidant and Antibacterial Activities against Planktonic and Biofilm Cultures of Vibrio spp. Strains. Molecules. 2015 ; 20 : 14402-24.   DOI
21 Pragadheesh VS, Yadav A, Chanotiya CS. Role of substituents in cyclodextrin derivatives for enantioselective gas chromatographic separation of chiral terpenoids in the essential oils of Mentha spicata. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 ; 1002 : 30-41.   DOI
22 Shmigol A, Kirischuk S, Kostyuk P, Verkhratsky A. Different properties of caffeine-sensitive Ca2+ stores in peripheral and central mammalian neurones. Pflugers Arch. 1994 ; 426 : 174-6.   DOI
23 Ferrazzano GF, Roberto L, Amato I, Cantile T, Sangianantoni G, Ingenito A. Antimicrobial properties of green tea extract against cariogenic microflora: an in vivo study. J Med Food. 2011 ; 14 : 907-11.   DOI
24 Araghizadeh A, Kohanteb J, Fani MM. Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria. Med Princ Pract. 2013 ; 22 : 368-72.   DOI
25 Souza FV, da Rocha MB, de Souza DP, Marçal RM. (-)-Carvone: antispasmodic effect and mode of action. Fitoterapia. 2013 ; 85 : 20-4.   DOI
26 Mun SH, Kang OH, Joung DK, Kim SB, Choi JG, Shin DW, Kwon DY. In vitro anti-MRSA activity of carvone with gentamicin. Exp Ther Med. 2014 ; 7 : 891-6.   DOI
27 Park KM, You JS, Lee HY, Baek NI, Hwang JK. Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens. J Ethnopharmacol. 2003 ; 84 : 181-5.   DOI
28 Browngardt CM, Wen ZT, Burne RA. RegM is required for optimal fructosyltransferase and glucosyltransferase gene expression in Streptococcus mutans. FEMS Microbiol Lett. 2004 ; 240 : 75-9.   DOI
29 Oda Y, Hayashi F, Okada M. Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in patients with intellectual disabilities. BMC Oral Health. 2015 ; 15 : 102.   DOI
30 Conrads G, de Soet JJ, Song L, Henne K, Sztajer H, Wagner-Döbler I, Zeng AP. Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level. J Oral Microbiol. 2014 ; 6 : 26189.   DOI
31 Tam A, Shemesh M, Wormser U, Sintov A, Steinberg D. Effect of different iodine formulations on the expression and activity of Streptococcus mutans glucosyltransferase and fructosyltransferase in biofilm and planktonic environments. J Antimicrob Chemother. 2006 ; 57 : 865-71.   DOI
32 Katsura H, Tsukiyama RI, Suzuki A, Kobayashi M. In vitro antimicrobial activities of bakuchiol against oral microorganisms. Antimicrob Agents Chemother. 2001 ; 45 : 3009-13.   DOI
33 Ren Z, Cui T, Zeng J, Chen L, Zhang W, Xu X, Cheng L, Li M, Li J, Zhou X, Li Y. Molecule Targeting Glucosyltransferase Inhibits Streptococcus mutans Biofilm Formation and Virulence. Antimicrob Agents Chemother. 2015.
34 Araghizadeh A, Kohanteb J, Fani MM. Inhibitory activity of green tea (Camellia sinensis) extract on some clinically isolated cariogenic and periodontopathic bacteria. Med Princ Pract. 2013 ; 22 : 368-72.   DOI
35 Subramaniam P, Eswara U, Maheshwar Reddy KR. Effect of different types of tea on Streptococcus mutans: an in vitro study. Indian J Dent Res. 2012 ; 23 : 43-8.   DOI
36 Crevelin EJ, Caixeta SC, Dias HJ, Groppo M, Cunha WR, Martins CH, Crotti AE. Antimicrobial Activity of the Essential Oil of Plectranthus neochilus against Cariogenic Bacteria. Evid Based Complement Alternat Med. 2015 ; 2015 : 102317.   DOI
37 Limsong J, Benjavongkulchai E, Kuvatanasuchati J. Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. J Ethnopharmacol. 2004 ; 92 : 281-9.   DOI