• Title/Summary/Keyword: dendrogram

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Rapid discrimination of commercial strawberry cultivars using Fourier transform infrared spectroscopy data combined by multivariate analysis

  • Kim, Suk Weon;Min, Sung Ran;Kim, Jonghyun;Park, Sang Kyu;Kim, Tae Il;Liu, Jang R.
    • Plant Biotechnology Reports
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    • v.3 no.1
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    • pp.87-93
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    • 2009
  • To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves and fruits of five commercial strawberry cultivars were subjected to Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Fisher's linear discriminant function analysis. The dendrogram based on hierarchical clustering analysis of these spectral data separated the five commercial cultivars into two major groups with originality. The first group consisted of Korean cultivars including 'Maehyang', 'Seolhyang', and 'Gumhyang', whereas in the second group, 'Ryukbo' clustered with 'Janghee', both Japanese cultivars. The results from analysis of fruits were the same as of leaves. We therefore conclude that the hierarchical dendrogram based on PCA of FT-IR data from leaves represents the most probable chemotaxonomical relationship between cultivars, enabling discrimination of cultivars in a rapid and simple manner.

Genetic Discrimination of Catharanthus roseus Cultivars by Multivariate Analysis of Fourier Transform Infrared Spectroscopy Data

  • Kim, Suk-Weon;Cho, Soo-Hwa;Chung, Hoe-Il;Liu, Jang-R.
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.201-205
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    • 2007
  • To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts of higher plants is applied to discriminate plants genetically, leaf samples of eight cultivars of Catharanthus roseus were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR fingerprint region data were analyzed by principal component analysis (PCA). Major peaks as biomarkers were identified as the most significant contributors to distinguish samples by using genetic programming. A hierarchical dendrogram based on the results from PCA separated the eight cultivars into two major groups in the same manner as the dendrograms based on genetic fingerprinting methods such as RAPD and AFLP. A slight difference between the dendrograms was found only in branching pattern within each subgroup. Therefore, we conclude that the hierarchical dendrogram based on PCA of the FT-IR data represents the most probable chemotaxonomical relationship between cultivars, which is in general agreement with the genetic relationship determined by conventional DNA fingerprinting methods.

Design of OTP based on Mobile Device using Voice Characteristic Parameter (음성 특징 파라메터를 이용한 모바일 기반의 OTP 설계)

  • Cha, Byung-Rae;Kim, Nam-Ho;Kim, Jong-Won
    • Journal of Advanced Navigation Technology
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    • v.14 no.4
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    • pp.512-520
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    • 2010
  • As the applications based on Mobile and Ubiquitous becoming more extensive, the communication security issues of those applications are appearing to be the most important concern. Therefore, every part of the system should be thoroughly designed and mutually coordinated in order to support overall security of the system. In this paper. we propose new technique which uses the voice features in order to generate Mobile One Time Passwords(OTPs). Voice is considered to be one of the powerful personal authentication factors of biometrics and it can be used for generating variable passwords for one time use. Also we performed a simulation of homomorphic variability of voice feature points using dendrogram and distribution of voice feature points for proposed password generation method.

Genetic Divergence Analysis among Micromutant Lines in Finger Millet(Eleusine coracana G.)

  • Muduli, Kumuda Chandra;Misra, Rama Chandra
    • Journal of Crop Science and Biotechnology
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    • v.11 no.1
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    • pp.63-68
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    • 2008
  • The induced genetic divergence was estimated in 44 mutant lines of finger millet variety GPU 26, developed by single and combination treatments with gamma rays, EMS and NG using three multivariate analyses. The mutant lines were grouped into eight genetically diverse clusters by multivariate D2 and canonical analyses and 11 clusters by dendrogram grouping through Gower's similarity coefficient. The clustering pattern in these three methods was almost similar. Twelve mutant lines in D2 and 13 in the dendrogram grouping method were grouped in the parental cluster(Cluster I) indicating that they did not possess enough divergence from the parent to be classified as micromutant lines. However a large proportion of mutant lines showed divergence from the parent variety and also among themselves. No definite relationship of mutagenic origin and clustering of mutant lines were observed. The mutant lines developed from the same mutagenic treatments often grouped into different clusters indicating that each mutagenic treatment was effective in inducing diverse types of changes in the nine traits studied. The hybridization program between the divergent mutant lines GE 2-2 or GE 3-4 with GG 3-1 is expected to give promising and desirable segregants in subsequent generations. Traits such as days to 50% flowering and days to maturity had major contributions to the induced genetic divergence.

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An Mobile-OTP(One Time Password) Key and Simulation using Fingerprint Features (지문 특징을 이용한 모바일 일회용 암호키 및 시뮬레이션)

  • Cha, Byung-Rae;Kim, Yong-Il
    • Journal of Advanced Navigation Technology
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    • v.13 no.4
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    • pp.532-543
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    • 2009
  • As the applications within Internet and Ubiquitous becoming more extensive, the security issues of those applications are appearing to be the most important concern. Therefore, every part of the system should be thoroughly designed and mutually coordinated in order to support overall security of the system. In this paper, we propose new technique which uses the fingerprint features in order to generate Mobile One Time Passwords(OTPs). Fingerprint is considered to be one of the powerful personal authentication factors and it can be used for generating variable passwords for one time use. Also we performed a simulation of homomorphic graph variable of fingerprint feature point using dendrogram and distribution of fingerprint feature points for proposed password generation method.

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Availability Verification of Integration OTP Framework using Biometrics Information (바이오매트릭스 정보를 이용한 모바일 기반의 통합 OTP 프레임워크의 유효성 검증)

  • Cha, Byung-Rae;Kim, Nam-Ho;Kim, Jong-Won
    • Journal of Advanced Navigation Technology
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    • v.15 no.1
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    • pp.39-53
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    • 2011
  • As the applications within Mobile devices becoming more extensive, the mobile communication security issues of these applications and researches are appearing to be the most important concern. In this paper, we propose new integration OTP framework technique which uses the fingerprint and voice features of biometrics in order to generate Mobile One Time Passwords (OTPs) Token. The fingerprint and voice are considered to be one of the powerful personal authentication factors of biometrics and it can be used for generating variable passwords based on mobile environments for one time use. However, we performed a simulation of homomorphic variability of fingerprint and voice feature points using dendrogram and distribution of fingerprint and voice feature points for proposed password generation method, and verified validation of availability.

Genetic Distances and Variations of Three Geographic Hairtail Populations Identified by PCR Analysis

  • Yoon, Jong-Man
    • Development and Reproduction
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    • v.18 no.3
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    • pp.167-172
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    • 2014
  • In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamer primers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three hairtail populations. Here, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population, 55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number of shared loci by the three populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28 loci, with an average of 4 per primer, were observed in the CHP population. The hierarchical dendrogram point out three main branches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15 ~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals' CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantly related to CHINESE no. 19 (genetic distance = 0.430). Consequently, PCR analysis generated on the genetic data displayed that the geographic AHP population was widely separated from CHP population, while individuals of CHP population were fairly closely related to those of GHP population.

Comparison of Electrophoretic Isozyme Band Pattern of Pleurotus spp. in Korea -I. Homogeneous Gel- (한국산 느타리버섯(Pleurotus spp.)의 전기영동 Isozyme Band Pattern 비교 -I. Homogeneous Gel-)

  • Park, Yong-Hwan;Byun, Myung-Ok;Hiroshi, Fujii
    • The Korean Journal of Mycology
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    • v.16 no.2
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    • pp.87-94
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    • 1988
  • Electrophoretic isozyme patterns from mycelia, primordia, cap and stem of Pleurotus spp. collected in Korea were compared. Primordia, cap and stem of fruitbody showed very similar isozyme patterns but mycelial isozyme patterns were different from those of fruitbody. Isozyme patterns of malate dehydrogenase, acid phosphatase in Pleurotus ostreatus collected from different regions in Korea were similar but those of esterase, peroxidase, leucine amino peptidase and superoxide dismutase were different. Interspecific comparison of esterase isozyme patterns among Pleurotus ostreatus P. cornucopiae and, P. florida was very different and may be valuable subsidiary tool to conventional taxonomic techniques for identifying species of Pleurotus. A dendrogram by similarities of isozyme band pattern was presented.

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Genetic Relationships among Korean Adlay, Coix lachryma-jobi L., Landraces Based on AFLPs

  • Moon Jung-Hun;Jang Jung Hee;Park Jung Soo;Kim Sung Kee;Lee Kyung-Jun;Lee Sang-Kyu;Kim Kyung-Hee;Lee Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.2
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    • pp.142-146
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    • 2005
  • Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.

RAPD-PCR Analysis in Fusarium species (Fusarium 종에서의 RAPD-PCR분석)

  • 민병례;양연주;최영길
    • Korean Journal of Microbiology
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    • v.35 no.2
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    • pp.107-114
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    • 1999
  • To assess genetic diversity amoug 21 strains from sixleen Frrsn~i~nn species , we used RAPD(rando1n amplified pol.ymorphic DNA) analysis based on PCR(po1ymerase chain reaction). Eleven primers showing Ule polymorphism were chosen from the 40 random pnmers-tcstcd. A total of 263 polymorphic bands were generated by the primers and the size of amplified DNA fragments ranged from 0.1 lo 3.0 kb. Sirnilku-it), coefficients between strains were calcnlatcd, and UPGMA cluster analysis was used to generate a dendrogram showing relationships among them. The results from RAPD-PCR analysis were grouped into four main groups at the si~nilarity level of 0.627.

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