• Plant Biotechnology Reports
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• 제3권1호
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• pp.87-93
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• 2009

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves and fruits of five commercial strawberry cultivars were subjected to Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Fisher's linear discriminant function analysis. The dendrogram based on hierarchical clustering analysis of these spectral data separated the five commercial cultivars into two major groups with originality. The first group consisted of Korean cultivars including 'Maehyang', 'Seolhyang', and 'Gumhyang', whereas in the second group, 'Ryukbo' clustered with 'Janghee', both Japanese cultivars. The results from analysis of fruits were the same as of leaves. We therefore conclude that the hierarchical dendrogram based on PCA of FT-IR data from leaves represents the most probable chemotaxonomical relationship between cultivars, enabling discrimination of cultivars in a rapid and simple manner.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.201-205
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• 2007

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts of higher plants is applied to discriminate plants genetically, leaf samples of eight cultivars of Catharanthus roseus were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR fingerprint region data were analyzed by principal component analysis (PCA). Major peaks as biomarkers were identified as the most significant contributors to distinguish samples by using genetic programming. A hierarchical dendrogram based on the results from PCA separated the eight cultivars into two major groups in the same manner as the dendrograms based on genetic fingerprinting methods such as RAPD and AFLP. A slight difference between the dendrograms was found only in branching pattern within each subgroup. Therefore, we conclude that the hierarchical dendrogram based on PCA of the FT-IR data represents the most probable chemotaxonomical relationship between cultivars, which is in general agreement with the genetic relationship determined by conventional DNA fingerprinting methods.

• 한국항행학회논문지
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• 제14권4호
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• pp.512-520
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• 2010

유비쿼터스와 모바일의 광범위한 응용과 더불어 통신 보안은 최근 중요한 관심사가 되고 있다. 따라서 각각의 보안 요소마다 다양한 기법 및 응용에 대한 연구와 시스템적 응용에 대한 연구가 활발히 이루어지고 있다. 본 논문에서는 음성의 특징을 이용한 모바일 OTP의 일회용 암호키를 생성하는 방법을 제안한다. 본 연구는 강력한 개인 인증에 사용되는 바이오매트릭스의 음성 정보를 이용하여 가변적이고 안전한 일회용 암호 키를 생성하였으며, 또한 제안 기법에 대한 덴드로그램(dendrogram)을 이용한 음성 특징점에 의한 준동형적(homomorphic) 가변성 그리고 음성 특징점의 분포를 시뮬레이션 하였다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.63-68
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• 2008

The induced genetic divergence was estimated in 44 mutant lines of finger millet variety GPU 26, developed by single and combination treatments with gamma rays, EMS and NG using three multivariate analyses. The mutant lines were grouped into eight genetically diverse clusters by multivariate D2 and canonical analyses and 11 clusters by dendrogram grouping through Gower's similarity coefficient. The clustering pattern in these three methods was almost similar. Twelve mutant lines in D2 and 13 in the dendrogram grouping method were grouped in the parental cluster(Cluster I) indicating that they did not possess enough divergence from the parent to be classified as micromutant lines. However a large proportion of mutant lines showed divergence from the parent variety and also among themselves. No definite relationship of mutagenic origin and clustering of mutant lines were observed. The mutant lines developed from the same mutagenic treatments often grouped into different clusters indicating that each mutagenic treatment was effective in inducing diverse types of changes in the nine traits studied. The hybridization program between the divergent mutant lines GE 2-2 or GE 3-4 with GG 3-1 is expected to give promising and desirable segregants in subsequent generations. Traits such as days to 50% flowering and days to maturity had major contributions to the induced genetic divergence.

• 한국항행학회논문지
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• 제13권4호
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• pp.532-543
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• 2009

인터넷 및 유비쿼터스의 광범위한 응용과 더불어 인터넷 보안은 최근 중요한 관심사가 되고 있다. 따라서 각각의 보안 요소마다 다양한 기법 및 응용에 대한 연구와 시스템적 응용에 대한 연구가 활발히 이루어지고 있다. 본 논문에서는 지문의 특징을 이용한 모바일 OTP의 일회용 암호 키를 생성하는 방법을 제안한다. 본 연구는 잘 알려진 강력한 개인 인증요소인 지문 정보를 이용하여 가변적이고 안전한 일회용 암호 키를 생성하였으며, 또한 제안 기법에 대한 dendrogram을 이용한 지문 특징점의 준동형 그래프간의 가변성 그리고 지문 특징점의 분포를 시뮬레이션을 통해 성능을 분석하였다.

• 한국항행학회논문지
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• 제15권1호
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• pp.39-53
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• 2011

모바일 장치의 광범위한 응용과 더불어 통신 보안과 연구가 최근 중요한 관심사가 되고 있다. 본 논문에서는 바이오매트릭스의 지문과 음성의 특징을 이용한 모바일 통합 OTP의 일회용 암호 키 토큰을 생성하는 방법을 제안한다. 강력한 개인 인증에 사용되는 바이오매트릭스의 지문과 음성 정보를 이용하여 모바일 환경의 가변적이고 안전한 일회용 암호 키를 생성하는 OTP 프레임워크를 제안하였으며, 또한 제안 기법에 대한 dendrogram을 이용한 지문과 음성 특징 점에 의한 준동형적 가변성 그리고 지문과 음성 특징 점의 분포를 시뮬레이션 하여 유효성을 검증하였다.

• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.167-172
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• 2014

In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamer primers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three hairtail populations. Here, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population, 55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number of shared loci by the three populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28 loci, with an average of 4 per primer, were observed in the CHP population. The hierarchical dendrogram point out three main branches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15 ~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals' CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantly related to CHINESE no. 19 (genetic distance = 0.430). Consequently, PCR analysis generated on the genetic data displayed that the geographic AHP population was widely separated from CHP population, while individuals of CHP population were fairly closely related to those of GHP population.

• 한국균학회지
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• 제16권2호
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• pp.87-94
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• 1988

한국산 느타리버섯 균주의 균사, Primordia, 갓, 줄기의 전피영동 isozyme 패턴을 비교한 결과 자실체중 Primordia, 갓, 줄기의 패턴은 모두 아주 유사하였으나 균사의 패턴은 자실체의 것과 많은 차이가 있었다. 자생지역이 다른 한국산 Pleurotus ostreatus의 전기영동 isozyme 패턴중 Malate Dehydrogenase, Acid phosphatase는 유사하였으나 Esterase, Peroxidase, Leucine amino peptidase, Superoxide Dismutase의 패턴은 차이가 있었다. Pleurotus osteatus, P. cornucopiac, P. florida의 종간 Esterase 밴드 패턴은 많은 차이가 있어 전기영동에 의한 느타리버섯 종의 가능하였다.

• 한국작물학회지
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• 제50권2호
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• pp.142-146
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• 2005

Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.

• 미생물학회지
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• 제35권2호
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• pp.107-114
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• 1999

Fusarium 균에 속하는 16종 21균주를 대상으로 RAPD-PCR 방법을 이용하여 DNA 다형성을 분석하여 계통 유전학적 유연관계를 검토하였다. 40개의 random primer 로 시험하여 실험한 모든 종에서 다형성을 나타내는 11개의 primer를 선별하였다. RAPD 분석결과 평균 23.9개씩 모두 263개의 크기가 다른 RAPD 밴드들을 조사할 수 있었다. 각 primer에 대해 각각 독특한 DNA 다형성을 나타내었고, 증폭된 DNA 크기는 0.1-3.0 kb 범위에서 형성되었다. 각 균주간의 genetic similarity를 계산하여 유연관계를 dendrogram 으로 나타내었다. Genetic similarity 0.627을 기준으로 하여 크게 4그룹으로 나눌 수 있었다.