• Archives of Pharmacal Research
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• 제27권12호
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• pp.1270-1274
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• 2004

Random amplified polymorphic DNA (RAPD) analysis was used to determine the genetic relationships among seventeen species of the Acanthopanax species. The DNA isolated from the leaves of the samples was used as template in polymerase chain reaction (PCR) with twenty random decamer primers in order to distinguish plant subspecies at the level of their genomes. The RAPD patterns were compared by calculating pairwise distances using Dice similarity index, and produced to the genetic similarity dendrogram by unweighted pair-group method arithmetic averaged (UPGMA) analysis, showing three groups; a major cluster(twelve species), minor cluster (4 species) and single-clustering species. The results of RAPD were compatible with the morphological classification, as well as the chemotaxonomic classification of the Acanthopanax species. The Acanthopanax species containing 3,4-seco-lupane type triterpene compounds in their leaves corresponded to the major cluster, another species having oleanane or normal lupane type constituents to minor clusters, and one species not containing triterpenoidal compound to single-cluster.

• 한국지능시스템학회논문지
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• 제13권5호
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• pp.512-519
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• 2003

본 논문에서는 계층적 클러스터링과 GMM을 순차적으로 이용하여 최적의 파라미터를 추정하고 이를 뉴로-퍼지 모델의 초기 파리미터로 사용하여 모델의 성능 개선을 제안한다. 반복적인 시도 중 가장 좋은 파라미터를 선택하는 기존의 알고리즘 과 달리 계층적 클러스터링은 데이터들 간의 유클리디언 거리를 이용하여 클러스터를 생성하므로 반복적인 시도가 불필요하다. 또한 클러스터링 방법에 의해 퍼지 모델링을 행하므로 클러스터와 동일한 갯수의 적은 규칙을 갖는다. 제안된 방법의 유용함을 비선형 데이터인 Box-Jenkins의 가스로 예측 문제와 Sugeno의 비선형 시스템에 적용하여 이전의 연구보다 적은 규칙으로도 성능이 개선되는 것을 보였다.

• 한국패류학회지
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• 제14권1호
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• pp.33-40
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• 1998

A horizontal starch gel electrophoresis for enzyme proteins extracted from 2 species of Korean viviparid snails; Cipangopaludina chinensis malleata and C. japonica was carried out in order to elucidate their genetic relationships. A total of 10 enzymes were employed in three different kinds of buffer systems. Two loci from each enzyme of alcohol dehydrogenase, esterase, glucose phosphate isomerase, isocitrate dehydrogenase, iditol dehydrogenase, malate dehydrogenase and peptidase(VL); and only one locus dach from two enzymes, glycerlo-3-phosphate dehydrogenase and phosphoglucomutase were detected; but, four loci from peptidase(LGG) were observed. Most of loci in two viviparid species showed homozygous monomorphic banding patterns and some of them were specific as genetic markers between two different species. However, EST-1, MDH-1, PEP(VL)-1loci showed polymorphic banding patterns. Foru populations of C. chinensis malleata were more closely clustered in a dendrogram within the range of genetic identity values of 0.928-1.00, and these clusters were lineated with C. japonica at the value of 0.355. In summarizing the above results, two viviparid snail species dmployed in this study mostly showed monomorphic enzyme protein banding patterns, and genetic differences specific between two species.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2010년도 학술발표회
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• pp.1355-1359
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• 2010

The purpose of this study is to analyze the spatial characteristics of Namgang-Dam watershed for a Total Maximum Daily Loads(TMDL). Three TMDL target sites, Gyeonghogang1, 2, Namgang-Dam2, are located within Namgang-Dam watershed. Under the current criterion for TMDLs, 3-year arithmetic mean BOD concentration of the target sites should not exceed the target concentration for 2 consecutive years. Two and three times of violation were observed for Gyenghogang2 and Namgang-Dam2 sites while no violation was found for Gyeonghogang1 site. However, no violation was found since 1999 for all three sites. Correlation between each 12 stations within the watershed were analyzed and cluster analysis was conducted to figure out the spatial characteristics of the watershed. Correlation coefficient between Gyonghogang1 and 2 was high (0.758) while the coefficients between lake station (Namgang-Dam2) and stream stations (Gyonghogang1 and 2) were very low. Dendrogram indicated that all of three Namgang-Dam stations were very close and Gyenghogang1, 2 stations were also close.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.854-858
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• 2005

A Lactobacillus isolate was collected from the feces of a healthy Korean individual and named as Lactobacillus ruminus SPM0211. It was further characterized by subjecting it to an antibiotic resistance test and genetic analysis. In the antibiotic resistance test, all tested Lactobacillus spp. were classified as 'high resistance' for multiple antibiotics, such as isoniazid, ethambutol, cycloserine, and vancomycin. L. ruminus SPM0211 was classified as 'high resistance' for streptomycin also, while the other tested Lactobacillus spp. were classified as low resistance. This suggests that the antimicrobial spectra may be a good indicator in the discrimination of this strain among the tested Lactobacillus spp. In a polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) analysis using the Microbial Uniprimer kit, L. ruminus SPM0211, and L. suebicus were clustered as a group with a 74.3% similarity level, suggesting that these two species are genetically related. Thus, our data suggest that the PCR-RADP method using the Microbial Uniprimer kit may be valuable in discriminating L. ruminus SPM0211 from other Lactobacillus spp.

• 한국정보과학회논문지:시스템및이론
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• 제30권10호
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• pp.544-555
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• 2003

DNA 마이크로어레이 실험으로 나오는 데이타는 아주 많은 양의 유전자 발현 정보를 담고 있기 때문에 적절한 분석 방법이 필요하다. 대표적인 분석 방법은 계층적 클러스터링(hierarchical clustering) 방법이다. 본 논문에서는 계층적 클러스터링의 결과로 나오게 되는 덴드로그램(dendrogram)에 대해서 후처리(post-Processing)를 시행함으로써 DNA 마이크로어레이 데이타 분석을 더 용이하게 해주는 리프오더링(leaf-ordering)에 대해서 연구하였다. 먼저, 기존의 리프오더링 알고리즘들을 분석하였고, 리프오더링 알고리즘의 새로운 접근 방식을 제안하였다. 또한 이에 대한 성능을 실험하고 분석하기 위해서 계층적 클러스터링과 몇 가지 리프오더링 알고리즘들, 그리고 제안된 접근 방식을 직접 구현한 HCLO (Hierarchical Clustering & Leaf-Ordering Tool)에 대해서 소개하였다.

• 대한지구과학교육학회지
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• 제9권1호
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• pp.65-87
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• 2016

The purpose of this study is to examine Korean journals related to science education in order to analyze research trends into Nature of science in Korea. The subject of the study is the level of Korean Citation Index (KCI-listed, KCI listing candidates), that can be searched by the key phrase, "Nature of science" in Korean language through the RISS service. In this study, the Descriptive Statistical Analysis Method is utilized to discover the number of research articles, classifying them by year and by journal. Also, the Sementic Network Analysis was conducted to Word Cloud Analysis the frequency of key words, Centrality Analysis, co-occurrence and Cluster Dendrogram Analysis throughout a variety of research articles. The results show that 91 research papers were published in 25 journals from 1991 to 2015. Specifically, the 2 major journals published more than 50% of the total papers. In relation to research fields., In addition, key phrases, such as 'Analysis', 'recognition', 'lessons', 'science textbook', 'History of Science' and 'influence' are the most frequently used among the research studies. Finally, there are small language networks that appear concurrently as below: [Nature of science - high school student - recognize], [Explicit - lesson - effect], [elementary school - science textbook - analysis]. Research topic have been gradually diversified. However, many studies still put their focus on analysis and research aspects, and there have been little research on the Teaching and learning methods.

• 한국약용작물학회지
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• 제20권6호
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• pp.440-446
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• 2012

Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon's information Index (S.I.) indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.

• 한국패류학회지
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• 제29권3호
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• pp.163-169
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• 2013

In the present study, the seven primers BION-33, BION-34, BION-37, BION-41, BION-44, BION-45 and BION-42 generated the total number of loci, average number of loci per lane and specific loci in Hongseong, Yeosu and Goheung population of F. mutica, respectively. 7 primers generated 19 specific loci in the Hongseong population, 29.3 in the Yeosu population and 23.1 in the Goheung population, respectively. Especially, the decamer primer BION-37 generated 7 unique loci to each population, which were identifying each population, approximately 700 bp in Hongseong population. In this study, the dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (HONGSEONG 01-HONGSEONG 07), cluster 2 (YEOSU 08-YEOSU 14) and cluster 3 (GOHEUNG 15-GOHEUNG 21). Among the twenty one cockles, the shortest genetic distance that displayed significant molecular differences was between individuals 17 and 19 from the Goheung population (genetic distance = 0.051), while the longest genetic distance among the twenty-one cockle individuals that displayed significant molecular differences was between individuals HONGSEONG no. 03 and YEOSU no. 12 (genetic distance = 0.616). Relatively, individuals of YEOSU population were fairly closely related to that of GOHEUNG population. Ultimately, PCR fragments revealed of in this study may be useful as a DNA marker the three geographic populations to distinguish.

• Mycobiology
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• 제47권1호
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• pp.76-86
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• 2019

Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, ${\beta}$-tubulin (TUB2), and translation elongation factor-$1{\alpha}$ ($TEF-1{\alpha}$) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and $TEF-1{\alpha}$ sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.