Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
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Genetic Diversity and Metabolite Analysis of Gastrodia elata by Inter-Simple Sequence Repeats (ISSR) Markers

ISSR 표지에 의한 천마의 유전 다양성분석 및 기능성 물질분석

  • Kim, Hyun Tae (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Kim, Ji Ah (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Park, Eung Jun (Division of Forest Biotechnology, Korea Forest Research Institute)
  • 김현태 (국립산림과학원 산림생명공학과) ;
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 박응준 (국립산림과학원 산림생명공학과)
  • Received : 2012.09.21
  • Accepted : 2012.11.16
  • Published : 2012.12.30
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Abstract

Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon's information Index (S.I.) indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.

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