• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.965-969
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• 1996

An investigation was carried out to elucidate the effects of type of ginseng, concentration of ginseng, and type of coagulants on the physical and sensory properties of soybean curd. The textural properties of soybean curd were not influenced by the type of ginseng. Soybean curd coagulated with glucono-delta-lactone (GDL) showed a greater hardness than that coagulated with other coagulants, whereas the former produced a lesser springiness than the latter. The L-value was proportionally reduced by the increase of ginseng level and soybean curd coagulated with $CaCl_2$ showed the lowest L value. All the curd products prepared with ginseng had a pale yellow color. In the sensory properties, springiness and beany taste of soybean curd linearly decreased as the concentration of ginseng was increased. The concentration of ginseng to improve the acceptability of ginseng soybean curd as determined by the physical and sensory evaluation, was less than 0.25%. The most acceptable ginseng soybean curd was the one coagulated with $MgCl_2$. Soybean curd prepared with GDL had the lowest acceptability because of its sour taste and textural properties.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.24-28
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• 2002

Effect of pretreatment processing (peeling time, drying temperature, sample type) on the quality of hot air drying peony roots was investigated. Peony roots were peeled for 0, 10, 20, 30, 60, 90 and 120 minute in the peeling machine after water washing. Sample types were whole or cut (thickness of 3mm). Temperatures of hot air drying were 30, 40, 50 and $60^{\circ}C$ The level of water contents in dried sample was $14{\sim}15%$. Drying time was muth reduced as the peeling time, cutting sample types and higher temperature. Contents of paeoniflorin were maintained highest amount (3.48%) in the non-peeling cut sample dried at $30^{\circ}C$ and lowest amount (2.29%) in the 120min-peeling whole one dried at $60^{\circ}C$. Skin color of dried peony roots was heavily discolored in the sample of non-peeling whole type dried at $60^{\circ}C$ and the color was dark brown $({\Delta}E\;was\;46.1)$. When considering shortening of drying time, preservation of active compounds and maintenance of color, the best pretreatment method of drying in peony roots was non-peeling, cut type processing.

• The Journal of Korean society of community based occupational therapy
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• v.8 no.2
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• pp.29-37
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• 2018

Objective : Sleep disorder caused by stress or disease to elderly currently. We tried to make clear constant exercise according to exercise intensity would have effect on sleep disorder in elderly using EEG and melatonin. Methods : Thirty subjects were over 65 years old who lived a senior facility in some parts. They were able to communicate and understand the purpose of the study. They also expressed their intension to participate actively in experiment. They should have alert consciousness and orientation about time, place, and people. Sleep disorder was assessed via below 6 hr total sleep time and Pittsburge Sleep Quality Index, which was satisfied with both of criterion at the same time. Exercises composed of low intensity walking, moderate aerobic exercise, and high intensity resistance strength. We used QEEG 8-System (LAXTHA Inc. KOREA) to check wave type and Polysomnograpy (Compumedics, Australia) to test quality of sleep. and Histologic features were observed by TTC (triphenyltetrazolium chloride) staining and H & E (Hematoxylin & Eosin) staining. Results : There was a significant sleep index and change of melatonin after aerobic exercise. There was a significant SOL, S1, S2, and SWS in aerobic exercise, but there was a significant SWS in walking and resistance strength. There was a significant change of delta wave especially in frontal and temporal region between exercises. Conclusion : They had different effects according to type of exercise, when elderly who have chemical, behavioral change of circadian rhythms did exercise consistently. Aerobic exercise had more effect on sleep disorder than other exercise. Therefore, we may supply proper exercise to elderly and high quality of life.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.373-381
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• 2019

Site-directed mutagenesis was employed to generate five different triple point mutations in the double mutant (C295A/I86A) of Thermoanaerobacter ethanolicus alcohol dehydrogenase (TeSADH) by computer-aided modeling with the aim of widening the small alkyl-binding pocket. TeSADH engineering enables the enzyme to accept sterically hindered substrates that could not be accepted by the wild-type enzyme. The underline in the mutations highlights the additional point mutation on the double mutant TeSADH introduced in this work. The catalytic efficiency ($k_{cat}/K_M$) of the ${\underline{M151A}}$/C295A/I86A triple TeSADH mutant for acetophenone increased about 4.8-fold higher than that of the double mutant. A 2.4-fold increase in conversion of 3'-methylacetophenone to (R)-1-(3-methylphenyl)-ethanol with a yield of 87% was obtained by using ${\underline{V115A}}$/C295A/I86A mutant in asymmetric reduction. The ${\underline{A85G}}$/C295A/I86A mutant also produced (R)-1-(3-methylphenyl)-ethanol (1.7-fold) from 3'-methylacetophenone and (R)-1-(3-methoxyphenyl)-ethanol (1.2-fold) from 3'-methoxyacetophenone, with improved yield. In terms of thermal stability, the ${\underline{M151A}}$/C295A/I86A and ${\underline{V115A}}$/C295A/I86A mutants significantly increased ${\Delta}T_{1/2}$ by $+6.8^{\circ}C$ and $+2.4^{\circ}C$, respectively, with thermal deactivation constant ($k_d$) close to the wild-type enzyme. The ${\underline{M151A}}$/C295A/I86A mutant reacts optimally at $70^{\circ}C$ with almost 4 times more residual activity than the wild type. Considering broad substrate tolerance and thermal stability together, it would be promising to produce (R)-1-(3-methylphenyl)-ethanol from 3'-methylacetophenone by ${\underline{V115A}}$/C295A/I86A, and (R)-1-phenylethanol from acetophenone by ${\underline{M151A}}$/C295A/I86A mutant, in large-scale bioreduction processes.

• Journal of the Korean Institute of Landscape Architecture
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• v.41 no.6
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• pp.52-61
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• 2013

This study was conducted to investigate the effects of pergola's shading on the thermal comfort index in the summer. The 3 type of pergolas($4m{\times}4m{\times}h2.7m$) which were screened overhead(I)/overhead west(II)/overhead west north(III) plane with reed blind for summer shading and winter wind break, were constructed on the 4th floor rooftop. Thereafter the meteorological variables(air temperature, humidity, radiation, and wind speed) of pergola I, III and rooftop were measured from 14 to 16 August 2013(1st experiment), those of pergola I, II and rooftop were measured from 26 to 28 August 2013(2nd experiment). The effects of pergola's shading on the radiation environment and mean radiant temperature($T_{mrt}$), standard effective temperature($SET^*$) were as follows. The maximum 1 h mean values of differences ${\Delta}$ of the sums of shortwave radiant flux densities absorbed by the human body (${\Delta}K_{abs,max}$) between pergola I, III and nearby sunny rooftop were $-119W/m^2$, $-158W/m^2$, those between pergola I, II and rooftop were $-145W/m^2$, $-159W/m^2$. The maximum 1 h mean values of differences ${\Delta}$ of the sums of long wave radiant flux densities absorbed by the human body (${\Delta}L_{abs,max}$) between pergola I, III and nearby sunny rooftop, were $-15W/m^2$, $-17W/m^2$, those between pergola I, II and nearby rooftop, were $-8W/m^2$, $-7W/m^2$. The response of the direction dependent long wave radiant flux densities $L_1$ on the pergola's shading turned out to be distinctly weaker as compared to shortwave radiant flux densities $K_1$. The pergola's shading leads to a lowering of $T_{mrt}$ and $SET^*$. The peak values of $T_{mrt}$ absorbed by the human body were decreased $16^{\circ}C$ and $21.4^{\circ}C$ under pergola I and III as compared to that of nearby rooftop in the 1st experiment. Those were decreased $18.8^{\circ}C$ and $20.8^{\circ}C$ under pergola I and II as compared to that of nearby rooftop in the 2nd experiment. The peak values of $SET^*$ absorbed by the human body were decreased $2.9^{\circ}C$ and $2.6^{\circ}C$ under pergola I and III as compared to that of nearby rooftop in the 1st experiment. Those were decreased $3.5^{\circ}C$ and $2.6^{\circ}C$ under pergola I and II as compared to that of nearby rooftop in the 2nd experiment. The relative $SET^*$ decrease in pergola II, III compared to nearby sunny rooftop $SET^*$ were lower than that in pergola I, revealing the influence of the wind speed. Therefore it is essential to design pergola to maximize wind speed and minimize solar radiation to achieve comfort in the hot summer. The $SET^*$ under pergola I, III were exceeded $28.7^{\circ}C$ and $30.4^{\circ}C$ which were the upper limit of thermal comfort and tolerable zone during all most daytimes in the 1st experiment(maximum air temperature $37.5^{\circ}C$). The $SET^*$ under pergola I was exceeded $28.7^{\circ}C$ which was the upper limit of thermal comfort zone at 13h, that under pergola II was exceeded $28.7^{\circ}C$ from 8h to 14h, meanwhile the $SET^*$ under pergola I, II were within thermal tolerable zone during most daytimes in the 2nd experiment(maximum air temperature $34.4^{\circ}C$). Therefore to ensure the thermal comfort of pergola for summer hottest days, pergola should be shaded with not only reed blind but also climbing and shade plants. $T_{mrt}$ and $SET^*$ were suitable index for the evaluation of pergola's shading effects and outdoors.

• Journal of Microbiology
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• v.44 no.6
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• pp.641-648
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• 2006

We previously reported that Skp1, a component of the Skp1-Cullin-F-box protein (SCF) complex essential for the timely degradation of cell cycle proteins by ubiquitination, physically interacts with Bfa1, which is a key negative regulator of the mitotic exit network (MEN) in response to diverse checkpoint-activating stresses in budding yeast. In this study, we initially investigated whether the interaction of Skp1 and Bfa1 is involved in the regulation of the Bfa1 protein level during the cell cycle, especially by mediating its degradation. However, the profile of the Bfa1 protein did not change during the cell cycle in skp1-11, which is a SKP1 mutant allele in which the function of Skp1 as a part of SCF is completely impaired, thus indicating that Skp1 does not affect the degradation of Bfa1. On the other hand, we found that the skp1-12 mutant allele, previously reported to block G2-M transition, showed defects in mitotic exit and cytokinesis. The skp1-12 mutant allele also revealed a specific genetic interaction with ${\Delta}bfa1$. Bfa1 interacted with Skp1 via its 184 C-terminal residues (Bfa1-D8) that are responsible for its function in mitotic exit. In addition, the interaction between Bfa1 and the Skp1-12 mutant protein was stronger than that of Bfa1 and the wild type Skp1. We suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex. The interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 1994.10a
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• pp.49-58
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• 1994

The methods of estimation of VTS effectivenss are compared and the worldwide literature related to the VTS effectiveness is reviewed. The review suggests three potential approaches ; simulation ; synthesis of expert opinion and statistical analysis of casualties. this study adopted dissimilar approaches to estimate the VTS effectiveness to the earlier studies ; the combination of synthesis of expert opinion and causal analysis of casualty. The VTS effectiveness is derived by multiplying casualty rate reduction factors by the effect level of causal factors. The development of casualty rate reduction factors was based on the questionnaire survey and the evolution of effect levels was based on the causal analysis using functional block diagram. According to these procedures the maximum benefit to be obtained through the introduction of a VTS system was approximately 46 percent overall. The collision reduction rate was estimated to be approximately 50 percent for a VTS system with advanced radar surveillance. And 47 percent of groundings 36 percent of rammings and 21 percent of founderings could be reduced by the introduction of VTS. These figures are more or less the same to the earlier studies. The VTS effectiveness by the different causal factor groups was examined. VTS may reduce about 68 percent of causal factors classified as environmental conditions 40 percent of human factors and 35 percent of technical factors in collision accidents. As a whole 60 percent of environmental factors 41 percent of human factors and 20 percent of technical factors may be prevented by a VTS. The key variable of the effectiveness percentage is the value of weight coefficient $\delta$. Therefore differing values for this input was discussed and the impact that these variations have on the VS effectiveness noted. As the results of sensitivity analysis of VTS effectiveness by $\pm$10 percent the effectiveness is varied approximately three to seven percent by casualty type. And the value is changed roughly four to eight percent by a $\pm$10 percent variation by different sub-areas.

• Polymer(Korea)
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• v.39 no.3
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• pp.370-381
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• 2015

In the system of poly(vinyl alcohol) (PVA)/dimethyl sulfoxide, the refractive index of polymer was very well matched to that of solvent and thus its scattered intensity could be minimized. After adding small amount of polystyrene latex particle (nominal diameter 200 nm), diffusion behavior of only probe particle was investigated against the concentration of polymer matrix by means of dynamic light scattering. The polymer concentration dependence of its reduced diffusion coefficient was able to be analysed with the stretched exponential function of the reduced concentration $C[{\eta}]$. In very dilute concentration regime, the depletion layer kept constant but at the early semi-dilute regime of $1{\leq}C[{\eta}]{\leq}2.5$, the concentration-dependent exponent of depletion layer ${\delta}$ was appeared to be -0.8 which was very close to theoretical one of -0.85. However it was also observed at the higher concentration that its layer thickness decreased more abruptly than theoretical expectation and this phenomenon was ascribed to Oosawa type attractive interaction between adjacent latex particles.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.3
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• pp.311-319
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• 2007

A Meshfree is a method used to establish algebraic equations of system for the whole problem domain without the use of a predefined mesh for the domain discretization. A point interpolation method is based on combining radial and polynomial basis functions. Involvement of radial basis functions overcomes possible singularity Furthermore, the interpolation function passes through all scattered points in an influence domain and thus shape functions are of delta function property. This makes the implementation of essential boundary conditions much easier than the meshfree methods based on the moving least-squares approximation. This study aims to investigate a stress analysis of structural element between a meshfree method and the finite element method. Examples on cantilever type plate, hollow cylinder and stress concentration problems show that the accuracy and convergence rate of the meshfree methods are high.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.9
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• pp.74-80
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• 2009

This paper describes a $2{\sim}6GHz$ CMOS frequency synthesizer that employs only one LC-tank voltage controlled oscillator (VCO). For wide-band operation, optimized LO signal generator is used. The LC-tank VCO oscillating in $6{\sim}8GHz$ provides the required LO frequency by dividing and mixing the VCO output clocks appropriately. The frequency synthesizer is based on a fractional-N phase locked loop (PLL) employing third-order 1-1-1 MASH type sigma-delta modulator. Implemented in a $0.18{\mu}m$ CMOS technology, the frequency synthesizer occupies the area of $0.92mm^2$ with of-chip loop filter and consumes 36mW from a 1.8V supply. The PLL is completed in less than $8{\mu}s$. The phase noise is -110dBC/Hz at 1MHz offset from the carrier.