• Animal cells and systems
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• v.4 no.1
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• pp.23-30
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• 2000

Horizontal starch gel electrophoresis for 29 populations (n=543) of the wrinkled frog, Rana rugosa, from Korea and Japan was peformed to assess the degree of genic variation and genetic diversity, and to understand the biogeographic pattern of distribution and speciation. A sum of 22 presumptive loci was screened from 17 enzymes and general proteins. Four loci, Aco, Est-3, Me-2, and Pgm, demonstrated high levels of polymorphism. The degree of average genetic variation of R. rugosa was P=22.7% (9.1-40.9%), Ho=0.086 (0.048-0.165) and He=0.090 (0.042-0.168). In the south-eastern region of the Korean peninsula (Chongsong, Yongchon, Ulsan, Kyongju, Pohang, yongdok and Ulchin), a few unique alleles in the Mpi locus were detected and their biogeographic implications were considered. The degree of genetic differentiation among the Korean populations was moderate (S=0.900), whereas the degree of genetic diversity between Korean and Japanese populations was notably high (S=0.687, D=0.293). This result corresponds with the data obtained by the mitochondrial cytochrome b gene sequence (Lee et al., 1999) suggesting that the Korean and Japanese R. rugosa might have evolved a specific level of genetic differentiation since their geographic isolation.

• Speech Sciences
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• v.1
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• pp.55-81
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• 1997

In the present paper, V-to-V coarticulation in Korean VCV sequences is discussed, focusing on links between consonantal production and degree of V-to-V coarticulation. Temporal and spatial differences between three types of Korean alveolar stops (lax /t/. aspirated /$t^h$/ and thense /t'/) are examined from VCV sequences involving all possible combinations of three Korean unrounded vowels /a, i,/ based on spectrographic and electrographic data(two male speakers and one female speaker and one female speaker respectively). Closure duration and voice onset time (VOT) were measured from acoustic data. 'Total duration', which is defined as the sum of the closure duration and the VOT, was also calculated in order to see the temporal distance between two vowels in a VCV sequence. Differences in lingual-palatal contact pattern at the maximum contact (MC) point between the three types of stop were observed from EPG data. V-to-V coarticulation was investigated by measuring the offset or onset of the second formant (F2) of the target vowels from spectrograms. Two different dimensions of articulation, temporal and spatial, seem to playa role in determining the degree of V-to-V coarticulation. The degree of V-to-V anticipatory coarticulation is influenced by the spatial characteristics of the intervening consonant while the degree of carryover coarticulation is influenced by the temporal characteristics of the consonant.

• Proceedings of the KWS Conference
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• 2009.11a
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• pp.102-102
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• 2009

The purpose of this study is to establish the predictive equation of transversal residual stress at the thick weldment of large container ship. In order to do it, the variables used for this study were restraint degree, yield strength of base material, thickness of weldment and welding heat input. Here, the level of restraint degree at the thick weldment of container ship having the various welding sequence was calculated using FEA. From the result, the h-type specimen was designed to simulate the level of restraint degree at the actual weldment of containership. With H-type test specimen designed, the effect of the variables on the distribution of transversal residual stress at the weldment in a container ship was evaluated using the comprehensive FEA. Based on the results, the predictive equations of mean value and the distribution of transverse residual stress in each location of residual stress were established using dimensional analysis and multiple-regression method. The validation of predictive equations was verified by comparing with measured results by XRD in the actual weldment of the ship.

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.367-374
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• 1997

Bovine rotaviruses(A, 288, 55086 strains) isolated from fecal samples in Korea were propagated onto MA104 cells and were confirmed tentatively as G6, G8, and G10, respectively, by RFLP analysis. Full-length VP7 gene of these isolates was amplified by reverse transcriptase polymerase chain reaction(RT-PCR) using VP7 specific primers and cloned into TA vector. Nucleotide and deduced amino acid sequences of VP7 genes of the isolates were determined and compared with those of bovine rotavirus reference strains(NCDV; G6, UK; G6, Cody I-801; G8 and B223; G10). A, 288 and 55086 isolates showed high degree of nucleotide sequence homology with NCDV and UK(93% and 94%), Cody I-801(86%) and B223(97%), respectively, However, they showed 71~74% of nucleotide sequence homlogy with bovine rotavirus reference strains which belong to different serotypes. From the results of deduced amino acid sequence homology analysis, three isolates showed 94~96% of homology with the same serotype reference strains but 80~84% of homology with the different serotype reference strains. Three bovine rotavirus isolates, A, 288 and 55086 strains, were confirmed as G6, G8, and G10, respectively, by nucleotide and deduced amino acid sequence analysis.

• Journal of applied mathematics & informatics
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• v.27 no.1_2
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• pp.49-58
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• 2009

For $1{\leq}k{\leq}r$, let ${\sigma}$($K_{r,r}$ - ke, n) be the smallest even integer such that every n-term graphic sequence ${\pi}$ = ($d_1$, $d_2$, ..., $d_n$) with term sum ${\sigma}({\pi})$ = $d_1$ + $d_2$ + ${\cdots}$ + $d_n\;{\geq}\;{\sigma}$($K_{r,r}$ - ke, n) has a realization G containing $K_{r,r}$ - ke as a subgraph, where $K_{r,r}$ - ke is the graph obtained from the $r\;{\times}\;r$ complete bipartite graph $K_{r,r}$ by deleting k edges which form a matching. In this paper, we determine ${\sigma}$($K_{r,r}$ - ke, n) for even $r\;({\geq}4)$ and $n{\geq}7r^2+{\frac{1}{2}}r-22$ and for odd r (${\geq}5$) and $n{\geq}7r^2+9r-26$.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.922-927
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• 2001

In an effort to regulate the mammalian cell behavior in entrapment with a gel, we have functionalized hydrogels with the putative cell-binding (-Arg-Gly-Asp-)(RGD) domain. An adhesion molecule of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, a cell recognition ligand, was induced into thermo-reversible hydrogels, composed of N-isopropylacrylamide with small amounts of acrylic acid (typically 2-5 $mol\%$ in feed), as a biomimetic extracellular matrix (ECM). The GRGDS containing a p(NiPAAm-co-AAc) copolymer gel was studied in vitro for its ability to promote the spreading and viability of cells by introducing a GRGDS sequence. Hydrogel with no adhesion molecule was a poor ECM for adhesion, permiting spreading of only $3\%$ of the seeded cells for 36h. By immobilizing the peptide linkage into the hydrogel, the conjugation of RGD promoted $50\%$ of proliferation for 36h. However, the GREDS sequence, nonadhesive peptide linkage, conjugated hydrogel showed only $5\%$ of the seeded cell for the same time period. In addition, with the serum-free medium, only GRGDS peptides conjugated to hydrogel was able to promotecell spreading, while there was no cell proliferation in the hydrogel without GRGDS. Thus, the GRGDS peptide-conjugated thermo-reversible hydrogel specifically mediated the cell spreading. This result suggests that utilization of peptide sequences conjugating with the cell-adhesive motifs can enhance the degree of cell surface interaction and influence the long-term formation of ECM in vitro.

• Environmental Analysis Health and Toxicology
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• v.24 no.2
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• pp.107-117
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• 2009

Metal pollution of aquatic ecosystems is a problem of economic and health importance. Information regarding molecular responses to metal exposure is sorely needed in order to identify potential biomarkers. To determine the effects of heavy metals on chironomids, the full-length cDNA of alcohol dehydrogenase (ADH3) from Chironomus riparius was determined through molecular cloning and rapid amplification of cDNA ends (RACE). The expression of ADH3 was analyzed under various cadmium and copper concentrations. A comparative and phylogenetic study among different orders of insects and vertebrates was carried out through analysis of sequence databases. The complete cDNA sequence of the ADH3 gene was 1134 bp in length. The sequence of C. riparius ADH3 shows a low degree of amino acid identity (around 70%) with homologous sequences in other insects. After exposure of C. riparius to various concentrations of copper, ADH3 gene expression significantly decreased within 1 hour. The ADH3 gene expression was also suppressed in C. riparius after cadmium exposure for 24 hour. However, the effect of cadmium on ADH3 gene expression was transient in C. riparius. The results show that the suppression of ADH3 gene by copper exposure could be used as a possible biomarker in aquatic environmental monitoring and imply differential toxicity to copper and cadmium in C. riparius larvae.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.11a
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• pp.16-18
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• 2011

This study aims to identify the degree of safety when mariners take their actions in several different situations. We have carried out many experiments in order to observe mariners' behavior, and then measured the safety level that is based on their actions to avoid dangerous collision situations. One of the most important actions that mariners have to take, either as their daily routine or when they are in a collision situation and then want to avoid that situation is the lookout. In this paper, behaviors on the lookout have been defined as a standard sequence of three steps that are "time of first detection", "time of recognition as risky vessel" and "time of starting avoiding action", and the suitability and applicability of the definition have been shown. And also we propose the risk assessment on the collision and the recommendation for reducing the collision at sea. Some analyzing results and the application of the results are reported. The sequence of lookout is also understood. By combining these knowledge and some systematic studies, we propose the risk assessment on the collision and the recommendation for reducing the collision at sea.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.327-330
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• 2004

Because of its effectiveness for the PD(partial discharge) pattern recognition, PSA(Pulse Sequence Analysis) has been considered as a new analytic method instead of conventional PRPDA(Phase Resolved Partial Discharge Analysis). However, PSA has a big problem that can misanalyze patterns in case of data missing resulting from poor sensitivity because it analyses the correlation between sequential pulses, which leads to hesitate to apply it to on-site. Therefore, in this paper, the problems of PSA such as data missing and noise adding cases were investigated. For the purpose, PD data obtained from various defects including noise adding data were used and analysed, The result showed that both cases can cause fatal errors in recognizing PD patterns. In case of the data missing, the error depends on the kinds of defect and the degree of degradation. Also, it could be noticed that the error due to adding noises was larger than that due to some data missing.

• Journal of Life Science
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• v.11 no.2
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• pp.83-86
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• 2001

The long terminal repeat (LTR) elements of human endogenous retrovirus (HERV) have been found to be coexpressed with genes located nearby. It has been suggested that the LTR elements have contributed to the genetic variation of human genome connected to various diseases. Recently, HERV-W family was identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using genomic DNAs derived from schizophrenia, we performed PCR amplification and identified six HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (87.7-99.5%) with HERV-W LTR (AF072500). Sequence analysis of the HERV-W LTR elements revealed that clone W-sch1 showed identical sequence with the AC003014 (PAC clone RP1-290B4) derived from human Xq23. Clone W-sch2 was closely related to the AC0072442 derived from human Y chromosome by phylogenetic analysis. Our data suggest that new HERV-W LTR elements in schizophrenia may be very useful for further studies to understand neuropsychiatric diseases.