• Journal of Sericultural and Entomological Science
• /
• v.27 no.1
• /
• pp.31-36
• /
• 1985

Using sexual differences between female and male as the indication of the degree of the physiological distrubance in the sex-limited larval marking strains or sex-limited egg colour strains, it was investigated whether the physiological distrubance caused y the translocated autosome fragment on the W-sex determination chromosome, have an effect on expression of the three quantitative characters; cocoon weight, cocoon shell weight, and cocoon shell percentage in the female silkworm. The mean values of the ratios of female to male the above two experimental groups were 127%, 107%, 85%, in cocoon shell weight, and cocoon shell percentage, respectively. On the other hand, those from the three normal stains, namely the basic stock strains, white egg strains, and hybrids were 129% for cocoon weight, 110% for cocoon shell weight, and 85% for cocoon shell percentage, respectively. From the results, it comes to the conclusion that the translocated autosome fragment on the W-chromosome has no influences on the expression of the quantitative characters of the female silkworm because sexual differences of the sex-limited strains were very similar to those of the three normal strains.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.49 no.1
• /
• pp.104-112
• /
• 2022

The purpose of this study is to analyze the fracture resistance of reattached tooth according to the resin materials and tooth preparation type under physiological conditions. Uncomplicated crown fracture in the oblique direction was reproduced on the extracted 64 anterior teeth. Depending on the composite resin material, reattachment was performed using a flowable resin and a packable resin. Depending on retentive forms, reattachment was performed using simple reattachment, 1.0 mm × 1.0 mm labial chamfer bevel, 1.0 mm × 1.0 mm lingual chamfer bevel and 1.0 mm × 1.0 mm circumferential bevel. A load was applied to the palatal surface of the tooth using a universal testing machine at an angle of 125 degree, which is the interincisal angle of normal children. Under the masticatory pressure condition, fracture resistance of lingual chamfer groups was 28.28 ± 7.41 MPa and 27.54 ± 4.45 MPa, which was significantly higher than those of simple reattachment groups, 17.21 ± 5.87 MPa and 20.10 ± 6.00 MPa, in both flowable and packable resin groups. When considering the lingual force similar to masticatory pressure, the fragment retention was significantly improved when the lingual chamfer was formed compared to the simple reattachment. Clinicians may consider the design of the lingual chamfer in order to improve fracture resistance to masticatory pressure during fragment reattachment.

• Journal of Biosystems Engineering
• /
• v.26 no.2
• /
• pp.131-140
• /
• 2001

This study was conducted to develop a garlic separator which could to reduce the labor in preparation of seeding. After consideration of the design criteria of a garlic separator such as no additional conveying device, simple construction and operation, enhancement of separating efficiency, reduction of damage, degree a rotating corn type garlic separator was designed. The effects of design parameters such as height and angle of the inner and outer corns, rotating speed of inner corn on the separating performance of the prototype were estimated. In performance was compared with manual work. The results are summarized as follows. 1. Garlic bulbs were separated by a spiral movement in the gap between inner rotating corn and outer fixed corn. At constant feed rate of garlic bulbs, the capacity of garlic separation increased with increase of rotating speed of inner corn. Especially, the capacity was very high at the rotating speeds of 300 and 400rpm. 2. The damage degree of separated garlics increased with rotating speed of corn within 10%. Above 300rpm, separability of Uisung garlic was about 100% and incomplete separation of Namdo garlic was within 2%. 3. The capacity of prototype garlic separator developed in this study was 30 times as large as that of human being.

• Animal cells and systems
• /
• v.5 no.2
• /
• pp.101-106
• /
• 2001

AFLPS (amplified fragment length polymorphisms) were used to estimate the genetic diversity of seven populations of Brassica campestis L. ssp. napus var. nippo-oleifera Makino between naturalized and cultivated populations. The seven Korean populations maintained a high level of genetic diversity. For example, all eight primers were high polymorphic, with an average of 3.2 effective alleles per primer set, and the expected heterozygosity was also high. The majority of genetic variance resided within populations The combinations of an insect-pollinated, outcrossing breeding system, large populations sizes, a high degree of gene flow and a propensity for high fecundity may explain the high level of genetic diversity within cultivated populations. Estimates of genetic similarity on the proportion of shared fragments ranged from 0.952 to 0.999. The high level of gene flow In Korean naturalized populations is mainly caused by seed dispersal via sea tide and the gene flow of cultivated populations may be enhanced in part by artificial pollen dispersal.

• Environmental Analysis Health and Toxicology
• /
• v.29
• /
• pp.7.1-7.8
• /
• 2014

Objectives The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. Methods A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was determined. DNA renaturation tendency was also investigated for the suggested denaturation method. Results Heating, beads mill, and sonication bath did not show any denaturation for 30 minutes. However probe sonication fully denatured DNA in 5 minutes. 1 mol/L sodium hydroxide (alkaline treatment) and 60% dimethyl sulfoxide (DMSO) treatment fully denatured DNA in 2-5 minutes. Conclusions Among all the physical methods applied, the direct probe sonication was the most effective way to denature the DNA fragments. Among chemical methods, 60% DMSO was the most adequate denaturation method since it does not cause full renaturation during DNA hybridization.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 1994.06a
• /
• pp.75-85
• /
• 1994

Biocontrol is playing a more and more important role in plant disease management. Evidences show that there are optimum prospects for people to apply biocontrol approach to control plant disease or to study the mechanism of antagonism.“The study of Antagonistic Protein of Bacillus spp.to Xanthomonas oryzae pv. oryzae”has been worked in our laboratory since 1986. One hundred and thirty antagonistics bacteria were screened out, most of them belonged to Bacillus spp., and showed very strong inhibitive effect to various plant pathogens. Nine antagonistic proteins (peptides) were purified (P11-I, P11-II, B8, B826-I, B826-II, A30-I, A30-II, G35). Two antagonistic protein related DNA fragments (B826-I, A30-II) were cloned and sequenced. B826-I DNA fragment composed by 905 bp, and it contained two ORF encoding 95, and 54 amino acids, respectively. By using Rifr and Kamr as the selective markers, we found the bacteria could colonize on rice leaf for at least 40 days. In greenhouse the antagonistic bacteria showed certain degree of control efficiency.

• Research in Plant Disease
• /
• v.29 no.4
• /
• pp.420-424
• /
• 2023

In 2020, within the Dongbaekdongsan area in Jeju Island, a Septobasidium sp. associated with a felt disease in Castanopsis sieboldii (Makino) Hatus. ex T. Yamaz. & Mashiba was identified. The symptom included the presence of brown, thin, and silk-like mycelial mats attached to the tree's bark, displaying variations in size from large to small. To induce hyphal growth, the samples collected were incubated in a moist chamber, and the newly formed hyphae were subjected to genomic DNA extractions. The nucleotide sequences of the internal transcribed spacer and small subunit rDNA genes were determined, and molecular characteristics among the isolates were investigated through polymerase chain reaction-based restriction fragment length polymorphism analysis. This Septobasidium sp. exhibited distinct morphological and phylogenetic features compared to those that were previously reported in South Korea. Consequently, this strain is taxonomically classified as a provisionally novel species of Septobasidium. Furthermore, the observed felt disease exhibited a high degree of host specificity, as it was exclusively identified in C. sieboldii without occurrence in other tree species at the time of observation.

• Journal of the Korean Chemical Society
• /
• v.40 no.8
• /
• pp.557-564
• /
• 1996

FAB CAD MS/MS(Fast Atom Bombardment Collision Activated Dissociation Mass Spectrometry/Mass Spectrometry) was used to study different degree of bond stability according to the linkage positions of alkali cationized $(Na^+, Li^+, K^+, NH_4^+)$ stereoisomeric and synthetic oligosaccharides. The alkali metal cations were much more stable, requiring over -40 eV of collision energy vs. only -10 eV for the protonated forms. Of the cations, the potassium cationized trisaccharides were more stable than the others. They would not yield fragment ions under the conditions of collision available in triple quadrupole. Other cationized species exhibited decreasing stability in the series $Nap^+>Li^+>NH_4^+$ using 0.8 mTorr argon pressure in the collision cell. Metal cations of the oligosaccharides maintained charge principally on the amino sugar as shown by shift of all the fragment ions containing the amino sugar. The reason for the higher stability of the metal cationized form is the formation of crown ether-like bond around metal cations, N-acetyl group on GlcNAc and oxygens on fucose moiety. Depending on the metal sizes and the conformation of linkage-isomeric region, cationized species gave linkage dependent fragment patterns and exhibited stability in the series 1-6 > 1-4 > 1-3 linkage.

• Journal of Food Hygiene and Safety
• /
• v.18 no.4
• /
• pp.211-217
• /
• 2003

To discriminate between the genetically modified soybean processed foods which were Korean traditional foods as Beansprout, doenjang, Beancurd (Tofu) and the unmodified one. we had analyzed comparatively that the loss degree of inner DNA about denaturalization factors in process step as heat or pressure and decision of suitable PCR primer by size. As a result of having compared about ${\beta}$-actin was 160bp, 335 promotor was 130 bp and NOS terminator was 132 bp effective. As a result of having checked a loss degree of a gene, as for the bean curd, DNA was mostly preserved well, and the loss of DNA along the processing process was hardly observed by a processing process. Most DNA of beansprout have moved to trunk after germination stage, and the appropriate analysis part was judged as the trunk. And the doenjang showed a detection difference of DNA by an operation of an enzye among self-life periods. Besides, after 50days, insertion gene was destoryed entrely so that detection was not possible.

• Geomechanics and Engineering
• /
• v.33 no.6
• /
• pp.553-565
• /
• 2023

As the structure of broken rock mass is complex, with obvious discontinuity and anisotropy, it is generally necessary to reinforce broken rock mass using grouting in underground construction. The purpose of this study is to experimentally investigate the mechanical properties of broken rock mass after grouting reinforcement with consideration of the characteristics of broken rock mass (i.e., degree of fragmentation and shape) and a range of reinforcement methods such as relative strength ratio between the broken rock mass and cement-based grout stone body (λ), and volumetric block proportion (VBP) representing the volumetric ratio of broken rock mass and the overall cement grout-broken rock mass mixture after the reinforcement. The experimental results show that the strength and deformation of the reinforced broken rock mass is largely determined by relative strength ratio (λ) and VBP. In addition, the enhancement in compressive strength by grouting is more obvious for broken rock mass with spherical shape under a relatively high strength ratio (e.g., λ=2.0), whereas the shape of rock mass has little influence when the strength ratio is low (e.g., λ=0.1). Importantly, the results indicate that columnar splitting failure and inclined shear failure are two typical failure modes of broken rock mass with grouting reinforcement.