Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Analysis of Molecular Diversity in Castanopsis sieboldii with Felt Disease Caused by Septobasidium sp.

Septobasidium sp.에 의한 구실잣밤나무 고약병의 분자학적 다양성 분석

  • Geon-Woo Lee (Department of Forest Environment Science, Jeonbuk National University) ;
  • Sang-Tae Seo (Forest Entomology and Pathology Division, National Institute of Forest Science) ;
  • Byeongjin Cha (Department of Plant Medicine, Chungbuk National University) ;
  • Sang-Sub Han (Department of Forest Environment Science, Jeonbuk National University)
  • 이건우 (전북대학교 산림환경과학과) ;
  • 서상태 (국립산림과학원 산림병해충연구과) ;
  • 차병진 (충북대학교 식물의학과) ;
  • 한상섭 (전북대학교 산림환경과학과)
  • Received : 2023.12.04
  • Accepted : 2023.12.12
  • Published : 2023.12.31
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Abstract

In 2020, within the Dongbaekdongsan area in Jeju Island, a Septobasidium sp. associated with a felt disease in Castanopsis sieboldii (Makino) Hatus. ex T. Yamaz. & Mashiba was identified. The symptom included the presence of brown, thin, and silk-like mycelial mats attached to the tree's bark, displaying variations in size from large to small. To induce hyphal growth, the samples collected were incubated in a moist chamber, and the newly formed hyphae were subjected to genomic DNA extractions. The nucleotide sequences of the internal transcribed spacer and small subunit rDNA genes were determined, and molecular characteristics among the isolates were investigated through polymerase chain reaction-based restriction fragment length polymorphism analysis. This Septobasidium sp. exhibited distinct morphological and phylogenetic features compared to those that were previously reported in South Korea. Consequently, this strain is taxonomically classified as a provisionally novel species of Septobasidium. Furthermore, the observed felt disease exhibited a high degree of host specificity, as it was exclusively identified in C. sieboldii without occurrence in other tree species at the time of observation.

2020년 제주도 동백동산 내 구실잣밤나무 수피에 고약병과 관련된 Septobasidium sp.가 발견되었다. 분리한 균주는 습실 처리하여 새로 생성된 균사에 대한 genomic DNA를 추출한 뒤 internal transcribed spacer 및 small subunit rDNA 유전자에 대해 염기서열을 밝혔으며 polymerase chain reaction-based restriction fragment length polymorphism 분석을 통해 균주들간의 분자학적 특성이 조사되었다. 이 새로운 Septobasidium sp.은 기존에 알려진 고약병들과 형태학적 및 계통학적으로 다르게 나타나 새로운 종으로 보고한다. 또한 이 고약병은 관찰 당시 주변의 다른 수목들에서는 발생하지 않고 오직 구실잣밤 나무에서만 나타나는 특성으로 기주특이성이 매우 높다는 것이 확인되었다.

Keywords

Acknowledgement

This work was supported by the research grant of the Chungbuk National University.

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