• Journal of the Korean association of regional geographers
• /
• v.18 no.3
• /
• pp.253-267
• /
• 2012

The study looked into the type and characteristics of debris landforms in Mt. Mudeung. By focusing on the representative area, we aimed to categorize the debris landforms based on the morphologic and genetic characteristcis. The types of debris areas in Mt. Mudeung can be divided into the exposed debris type, mixed type of matrix, and the boulder-hidden type. Supply of block in the debris slope area displays different features depending on types of rocks. For the stony slopes of andesite, the block must be moved from the columnar joint or cliff in the upper part. The andesite debris slopes display dominant edge shape while displaying no round shape. The granite stony slopes display dominant round shape and the present exposed slope was assumed to be formed as the core stone which was deep weathered moved along slope during the periglacial era and the matrix was removed after post-glacial era. The movements of blocks are assumed to be caused by solifluction process. The joint area where granite and andesite areas meet, granite is located beneath andesite area, and this implies that blocks were actively freezing and creeping by solifluction and freezing and thawing at that time. It can be assumes that the granite matrix formed plain slope and then andesite boulder covered up the slope. Currently, the blocks in the stony slopes of Mt. Mudeung shows almost no mobility and the stony slopes created under periglacial climate can be considered to be fossil landform.

• Journal of Soil and Groundwater Environment
• /
• v.11 no.5
• /
• pp.43-50
• /
• 2006

Bioreporter bacteria, such as recombinant bioluminescent bacteria, have been used for the detection of specific compounds in complex environmental media. In this study, optimum conditions for the preparation and application of deep-freezed and Iyophilized recombinant bioluminescent strain KG1206 were investigated for the future application on contaminated environmental sites. Genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, contains TOL plasmid and the plasmid inserted $P_{m}$, promoter on the upper part of lux gone in vector pUCD615, and m-toluate and benzoate are considered direct inducers for bioluminescence. Optimum conditions determined for the preparation and application of the deep-freezed and lyophilized strain were followings: cryoprotective agent (24% sucrose), lyophilization time (12 hrs), strain concentration ($OD_{600}=0.6$), reconstitution for freezed strain (quick reconstitution at $35^{\circ}C$), reconstitution for lyophilized strain ($3{\sim}6$ hrs exposure on LB medium), carrying conditions (keep at $20^{\circ}C$ after reconstitution). These results demonstrate the feasibility of deep-freezed or lyophilized state of genetically engineered bioluminescent strain for environmental usage.

• Journal of fish pathology
• /
• v.34 no.2
• /
• pp.249-253
• /
• 2021

Anisakiasis is a well-known zoonosis caused by ingestion of raw or thermally undercooked seafood product contaminated with live Anisakis nematode third stage larvae (L3). Several traditional processing techniques have been used to kill or remove the Anisakis larvae worldwide, but thermal processing or deep freezing are the most effective treatments to kill the Anisakis larvae. In this study, we investigated the survival of Anisakis larvae in several condiments (soy bean sauce, wasabi, vinegar, red pepper paste) commonly consumed when eating raw fish in Korea. We also examined several different media (NaCl solution, absolute alcohol, soju) to investigate their larvicidal effect. When directly exposed to various condiments, the most effective larvicidal effect was observed in the mixture of wasabi and soy bean sauce. When exposed to different NaCl solutions, the larvicidal ability became more effective as the concentration increased, but did not show 100% killing effect. In soju, the L3 were killed under less than 4 hr. We observed the larvicidal effects of several condiments in this study, but these results are thought to be carefully interpreted for actual use because all the condiments in this study showed the effect in hours and in general, the L3 are exposed to these condiments only for seconds before ingested in real situation.

• Economic and Environmental Geology
• /
• v.53 no.4
• /
• pp.413-423
• /
• 2020

Over the wide area, King Sejong Station and the nearby land are uncovered with snow and ice conditions. Therefore, the active layer on the permafrost has been formed to be much thicker than the other Antarctica region. Electrical resistivity survey of Wenner and dipole-dipole arrays was undertaken at a series of time in the freezing season at the King Sejong Station to delineate subsurface structure and to monitor active layer in permafrost terrain. Time-lapse resistivity structures are well in terms of the vegetation distribution, ground surface temperature, and snow depth. Horizontal high resistivity belt(>1826 Ωm) at very shallow depth is thickening with the lapse of time, probably caused by the freezing of the water in the pore spaces with decrease of ground temperature. Subsurface structures for the area of low snow-cover and vegetated zone area are comprised of 0~0.5 m deep high-resistive gravel-rich soil, 0.5~3 m deep low-resistive active layer, and the underlying permafrost. In contrast, the unvegetated area and high snow-buildup is characterized with high resistivities larger than approximately 2000 Ωm due to freezing of the soil throughout the year. Data interpretation and correlation schemes explored in this paper can be applied to confirm the active layer, which is expected to get thinner in additional survey during the thawing season.

• Journal of Ginseng Culture
• /
• v.3
• /
• pp.1-10
• /
• 2021

Panax ginseng (Ginseng or Korean ginseng) is one of the most important medicinal herbs in the world. We made a high-quality whole genome sequence of P. ginseng using 'Chunpoong' cultivar, which is the first cultivar registered in Korea Seed and Variety Service (KSVS) with relatively similar genotypes and superior phenotypes, representing approximately 3 Gbp and 60,000 genes. Genome sequence analyses of P. ginseng and related speciesrevealed the origin of Korean ginseng and the ecological adaptation of 18 Panax species around the world. Korean ginseng and American ginseng (P. quinquefolius) are tetraploid species having 24 chromosome pairs, while the other 16 species are diploid species with 12 chromosome pairs. Panax and Aralia are the closest genera belonging to the Araliaceae family that diverged approximately 8 million years ago (MYA). All Panax species evolved as shade plants adapting to cool climates and low light conditions under the canopy of deep forests from Southeast Asia such as Vietnam to Northeast Asia such as Russia approximately 6 MYA. However, through recurrent ice ages and global warming, most diploid Panax species disappeared due to the freezing winter, while tetraploid P. ginseng may have appeared by allotetraploidization, which contributed to the adaptation to cold temperaturesin Northeast Asian countries including the Korea peninsula approximately 2 MYA. American ginseng evolved by the adaptation of P. ginseng in Northeast America after the intercontinental migration 1 MYA. Meanwhile, most of diploid Panax species survived in high-altitude mountains over 1,600 meters in Southeast Asia because they could not endure the hot temperature and freezing cold. The genome sequence provides good basisto unveil the origin and evolution of ginseng and also supports practical gene chips which is useful for breeding and the ginseng industry.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.15 no.4
• /
• pp.303-316
• /
• 1993

The auricular cartilage grafts have been widely used in replacement of the temporomandibular joint disk. Cartilage grafts itself have a low metabolism and high survival rate after grafting. In processing the grafting materials, it was important to preserve the properties of chondrocyte proper. We used 15% glycerol and 10% DMSO (Dimethyl Sulfoxide) solutions for cartilage fixation before deep freezing. We have performed the allogenic auricular cartilage graft in the temporomandibular joint of 20 rabbits which 10 specimen was treated with 15% glycerol and the other 10 specimen was treated with 10% DMSO respectively and examined in 1, 2, 4, 6 and 8 weeks after operation histopathologically. The result were : 1. Inflammatory cell infiltration around the grafted material appeared more glycerol groups than DMSO groups at 1 week, but each group has no differences after 2 weeks. 2. Degenerative changes of grafted auricular chondrocytes were more deveolped in glycerol group than DMSO groups till 4 weeks, but there were no differences between two groups after 6 weeks. 3. Fibrous union between grafted fragment and mandibular condyle was prominent in DMSO group. 4. Vascular proliferation of the grafted auricualr cartilage was more developed in DMSO groups than glycerol group in early stage. 5. Amount of the additional growth of grafted auricular cartilage was more existed in DMSO groups than glycerol group. 6. General survival rate after grafting was more prominent in DMSO group. In summary, allogenic auricular cartilage grafts treated with 15% glycerol and 10% DMSO solution have supported to survivalbility as a cryopreservative agents, especially DMSO groups have little inflammatory cell infiltration in early stages and degenerative changes and additional growth are more prominent than glycerol groups.

• Korean Journal Plant Pathology
• /
• v.2 no.3
• /
• pp.150-157
• /
• 1986

Effects of presservation methods on viability and virulence of Xanthosmonas compestris pv. oryzae were investigated. The incidence of colony-type variants from freeze-dried and deep-frozen cultures was also determined. The suspending medium for freeze-dried cultures containing $10\%$ sucrose and $1\%$ gelatin showed the highest viability, and the virulence was well maintained in the suspending medium containing $2\%$ dextrin, $0.5\%$ ascorbic acid, 0.5% ammonium chloride, $0.5\%$ thiourea, and $0.85\%$ NaCl. Serially transferred cultures became attenuated. Rough colonies which had wrinkled surface occurred at a frequency of $1.9\%$ to $15.8\%$ during freeze-drying and freezing. The rough colonies consisted of nonseptated filamentous cells and rod-shaped cells. The virulence of rough colonies was weak as compared with the normal colony type.

• Journal of the Korean association of regional geographers
• /
• v.9 no.4
• /
• pp.534-545
• /
• 2003

The main purpose of this paper is to describe the granitic regolith and frost creep phenomena in Daegwanryung area in order to demonstrate the relationship of the weathered materials and the surrounding surface, in order to point out that the characteristics of this particular complex of granitic regolith most closely resemble those of soil frost creep phenomena formed elsewhere under periglacial conditions. For this study, the writer has clarified their structure and its associated milieux, with a group of methods and techniques, field survey, micro-morphological analysis, granulometry of weathered materials etc. Such facies of granitic regolith in situ with silty heterometric materials and micas are prone to be deformed by cryo-suction as well as deep freezing. It was probably formed by cryogenic activity, or frost creep in the periglacial climate of a very cold and humid condition.

• The Korean Journal of Quaternary Research
• /
• v.3 no.1
• /
• pp.35-45
• /
• 1989

In Korean peninsula as well as in Western European Countries, we can find, reddish bands in sandy deposits of various origin. These bands (called "$B_t$ Band"), composed of fine material, are regularly intervalled and parallel to topography. This study is tring to elucidate their origin and associated milieux, with a group of methods and techniques (field survey, micromorphological analysis, granulometry, X-ray diffractometry of clay minerals...). $B_t$ Bands are not sedimentological origin. They were formed by superposition of two type accumulations in the sandy profile, triggered by different mechanisms: 1) accumulation in banded form, concerning silty heterometric material and micas, oweing to cryophoretic forces operating in course of deep freezing of sandy deposits: 2) illuviation of ferrugineous clays in the precedants bands, migrated in chelate state by soil water. The first processes are associated with very cold and humid climate in which freeze soil more than 3m in depth. The second, cold and humid climate in which podzolizattion is generalized. In case of South Korea and Western Europe, these two types of climates are not present circumstance, but Recent Quaternary (probably since the Wurm).the Wurm).

• Korean Journal of Animal Reproduction
• /
• v.7 no.1
• /
• pp.19-23
• /
• 1983

Present studies were conducted to investigate the developmental stage and the location of embryos in the reproductive tract at various times after ovulation, the morphologically normal after thawing of embryos preserved in liquid nitrogen, and the survival after transferring frozen-thawed embryos. The results obtained were as follows: 1. Embryo stage and location in the reproductive tract after hCG administration. For the investigation of embryo stage and location in the reproductive tract after ovulation, rabbits were laparotomized at 24, 40, 48, 72 and 120 hrs post hCG injection, simultaneously with mating. the oviducts and uteri were flushed out with PBS medium containing 50% rabbit serum, respectively. 1) Most of embryos was remained in the oviduct within 48 hrs, with the lapse of time, embryos were started to move to uterus and shifted in uterus at 72 hrs after hCG injection. 2) The representatives of embryos stage collected at 24, 40, 48, 72 and 120 hrs were 1-cell(60.4%), 8-cell to early morula (52.3, 39.3%), late blastocyst (95.5%) stages, respectively. 2. Morphological normality and survival of the frozen-thawed embryos. For the evalution of the quality and viability on the frozen-thawed embryos, immediately after thawing, embryos were assessed by morphologically normal under a dissecting microscope, and a further test of frozen-thawed embryos was made by transferring the morphologically normal embryos to the uteri of recipient rabbit induced pseudopregnancy by the injection of hCG at the time of hCG injection in donor rabbits. 1) The propotions of embryos which a, pp.ared morphologically normal was higher when 8-cell (85.7%) and morula(90.5%) were used for freezing than when 4-cell (66.7%) and blastocyst (75.8%) were used. 2) Preganacies were observed at Day 15 after transfer of frozen-thawed 8-cell (7/13), morula (19/42) and blastocyst (3/19) but not after transfer of embryos at 4-cell stage.