• 대한예방한의학회지
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• 제17권3호
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• pp.177-185
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• 2013

Objective : Eucommiae Cortex is bark of Eucommia ulmoides Oliver, a deciduous tree native to China. Today, teas or herbal drugs made from E. ulmoides leaves are widely consumed as a healthy food especially in Eastern Asia including Japan, China and Korea. This study was aimed to verify the extraction efficiency of bioactive compounds from Eucommiae Cortex depending on characteristics of decoction water. Method : Six types of decoction water with various origin were prepared and the contents of minerals in water were analyzed by ICP-AES and ICP-MS. The contents of extracted bioactive compounds were analyzed by HPLC and the relationship between mineral contents and extracted bioactive compounds in the decoction water were analysed by multiple factor analysis. The decoction waters could be classified in three groups by hierarchical cluster analysis. Result : Extracted amounts of compounds in Eucommiae Cortex showed a positive relationship with Na, Si, K concentration in decoction water, while a negative relationship with Ca, Mg concentration. Conclusion : The study proved that the minerals in water influenced the extraction efficiency of bioactive compounds from Eucommiae Cortex.

• 생약학회지
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• 제43권1호
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• pp.16-21
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• 2012

The characteristics of decoction water are important for the extraction of herbal drugs. In this study, the relationship between the characteristics of water and extraction efficiency of bioactive compounds from Corydalis tuber was investigated. Six different types of decoction water, including three types of mineral water from Jeju Island and three types of bottled water sold in market, were used as decoction water for the extraction of bioactive compounds from Corydalis tuber. The contents of minerals in six types of water were analyzed by ICP-AES and ICP-MS; the contents of extracted bioactive compounds were analyzed by HPLC. Multivariate analysis of variance was used to analyze the differences in total extraction and bioactive compounds extracted with six types of water. Multiple factor analysis was used to analyze the relationship among the pH, content of mineral, anion, total extractions and content of bioactive components as factors. There was a significant difference among the six types of water in the total extracts and the bioactive compounds. The results proved that the content of extracted compounds from Corydalis tuber was influenced by the types of decoction water.

• 대한본초학회지
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• 제25권1호
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• pp.9-12
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• 2010

Objectives : To evaluate the extraction efficiency of water decoction with or without non-woven fabric underlying comparison of the HPLC (high-performance liquid chromatography) profiles of the hesperidin which is the standard of Citri Pericarpium. Methods : Pyungwisan, which is comprised of Atractylodis Rhizoma, Magnoliae Cortex, Citri Pericarpium, Glycyrrhizae Radix, Zingiberis Rhizoma, and Ziziphy Fructus, was extracted in boiling water with non-woven fabric or without non-woven fabric. Then hesperidin content was analyzed by HPLC. Results : The contents of hesperidin in the water extract of Pyungwisan which was decocted without non-woven fabric showed $219.57{\pm}2.51\;mg/g$ and $138.90{\pm}6.32\;mg/g$ in with non-woven fabric decoction. Conclusions : These results suggest that herbal decoction without non-woven fabric might increase extraction efficiency.

• 대한본초학회지
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• 제27권6호
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• pp.63-69
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• 2012

Objectives : Decoction, in Korean Medicine, is a pharmacological method of extraction, by boiling, of dissolved chemicals, or herbal prescriptions, which may include stems, roots, bark and rhizomes. Decoctions differ from most teas, infusions, in that they are usually boiled. This study was performed to compare the difference of water decoctions extracted by different decoction extractor and extraction time and to analyze the reason of decoctions extracted by each decoction extractor have different taste. Methods : With water decoction samples by Pressure extractor, Non-pressure extractor and Ultrasonic waves merge extractor for 1 hr, 2 hr and 3 hr were investigated the yield and the concentration of hesperidin and glycyrrhizin by HPLC/DAD system in Pyungwi-san decoction. Results : The samples of each extractor were gradually increased the yield and the concentration of hesperidin and glycyrrhizin. The HPLC pattern of samples is similar. The yield and the concentration of hesperidin and glycyrrhizin of Ultrasonic waves merge extractor was most highest of the three. The rate of increase of the yield and the hesperidin concentration of between 1 hr and 2 hr in Pressure extractor was the most highest of the three. But the concentration of glycyrrhizin in Pressure extractor was relatively similar to Non-pressure extractor. Conclusions : The yield and the concentration of reference compounds in Pyungwi-san water decoction was influenced by extracting method and extracting time. For scientific movement and standardization of extracting medicinal herbs method need to study of extractor validation and to study in vitro and in vivo.

• 분석과학
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• 제24권1호
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• pp.38-44
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• 2011

본 연구는 제주지역에서 생산되는 세 종류의 물과 서울지역에서 사용되고 있는 수돗물 등 네 종류의 물을 전탕용수로 하여 중요 한약재인 대황으로부터 활성성분을 추출하고 용수 중의 미네랄이 대황활성성분 추출에 미치는 효과에 대하여 비교 분석하였다. 유도결합플라즈마방출분광기와 유도결합플라즈마질량분석기를 이용하여 30여종의 미네랄과 다수의 음이온을 분석하였다. 각 전탕용수로 대황을 추출하고 그 추출액의 일부를 증발시켜 총 추출량을 구하고, 또한 HPLC를 이용하여 추출액으로부터 대황의 생리활성 성분을 분석하였다. 용수 간 총 추출량과 대황 성분함량의 차이 검정을 위하여 Kruskal-Wallis 검정을 시행하였고, 미네랄, 음이온, 총 추출량 및 성분함량과의 관계분석을 위하여 다중요인분석을 시행하였다. 분석결과 용수 중의 미네랄 함량은 대황의 총 추출량 및 성분 추출량과 높은 상관관계가 있는 것으로 나타났다.

• Toxicological Research
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• 제6권1호
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• pp.41-49
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• 1990

Aconiti Tuber is the root of Aconitum sp (Ranunclaceae) which has been considered as one of the most important medicinal plant having cordiotonic, diuretic and analgesic effect. On the other hand, it has been known that Aconiti Tuber contained toxic agent, aconitine alkaloids so that only processed Aconiti Tubers have been used as herbal drug traditionally. For the safety evaluation of processed Aconiti Tuber, quantitative determination of aconitine and acute, subacute toxicity test were performed on 5 commercial processed Aconiti Tubers. Arapid and precise method using HPLC has been developed for the separation and determination of aconitine. Samples were extracted with hydrochloric acid (pH3) and hot water decoction. In case of d-HCL extracts, the contents of aconitine were from 0.08 mg/g to trace. But in case of hot water decoction extracts, the contents of aconitine were not detected. For the investigation of Aconiti Tuber toxicity in rats, hot water decoction samples and methanol extracts were tested. 1) Acute toxicity test Hot water decoction sample and methanol extracts from Aconiti Tuber did not show any toxic effects in rats by an oral administration. $LD_50values of 2 extracts were above 10.0 g/kg. 2) Subacute toxicity study In the repeated administration study, hot water decoction samples were given orally to Sprague-Dawlay rats for 2 week at daily doses of 5.0 g/kg. The results are as follows; No toxic manifestation, body weight changes and lethality were observed during wxperimental period. There were no significant changes in serum enzyme activities such as GOT, GPT, LDH, ALP between treated and control groups. However CPK values were decreased in the Subuja-treated group. (P<0.01). In addition, no gross and microscopic changes were noted in Aconiti Tuber-treated groups.

• 대한한의학회지
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• 제24권1호
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• pp.133-140
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• 2003

Objectives : Kochiae Fructus (dried fruits of Kochia scoparia L. Schrad,) is used in the treatment of skin diseases as internal or external medicine in Oriental Medicine, but there has not been experimental study of Kochiae Fructus as an external medicine. The purpose of this study was to investigate if it has certain effects on the skin or not. Methods : By a skin prick test method, we evaluated the effects of wet dressing with Kochiae Fructus decoction in different concentrations on histamine-induced itch, erythema and wheal responses, and compared them with the effects of calamine lotion and distilled water in 8 healthy volunteers aged 26-32. Results : The mean intensity and duration of itch were most decreased after wet dressing with a Kochiae Fructus decoction of 100mg/150ml. The time required for erythema to reach the maximal size was around 5 minutes in all groups, and the size of erythema was smallest on the skin taking wet dressing with the Kochiae Fructus decoction of highest concentration. The size of wheal was also smallest after wet dressing with the highest concentration Kochiae Fructus decoction. The effects of wet dressing with Kochiae Fructus decoction on skin were dependent on their concentrations. Conclusions : Kochiae Fructus suppressed the histamine-induced skin responses, which supports the ancient herbal literatures which describe that Kochiae Fructus has a certain effectiveness on some skin diseases.

• 대한한의학방제학회지
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• 제16권2호
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• pp.145-153
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• 2008

Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by Chromosomalanomaly test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo Chromosomalanomaly test was performed using chiness hamster lung cell line. Results : Sipjeondaebo-tang water extract was negative in Chromosomalanomaly test at the doses of 0, 625, 1250 and $2500{\mu}g/m\ell$ at 6h and 24h.(S9- fraction). Chromosomalanomaly test(S+fraction) was also negative at the doses of 0, 1250, 2500 and $5000{\mu}g/m\ell$. Conclusions : It was concluded that Sipjeondaebo-tang extract did not induce Chromosomalanomaly in the chiness hamster lung cell.

• 대한한의학방제학회지
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• 제16권1호
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• pp.109-115
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• 2008

Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by micronucleus test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo micronucleus test was performed using 7 weeks ICR mice. At 24 hours after with Sipjeondaebo-tang extract at the doses of 0, 500, 1000 and 2000 mg/kg/day by peritoneal route mice were sacrificed and bone marrow cells were prepared for smear slides. Results : As a result of counting the micronucleus polychromate erythrocyte of 2000 polychromate erythrocyte, all treatment groups did not show statistically significant increase than negative control group. and there was no clinical sign and body weight connected with injection of Sipjeondaebo-tang(Shiquan dabu-decoction) extract. Conclusions: It was concluded that Sipjeondaebo-tang extract did not induce micronucleus in the bone marrow cells of ICR mice

• Journal of Acupuncture Research
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• 제32권4호
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• pp.109-118
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• 2015

Objectives : The purpose of this study was to investigate the anti-oxidative effect of Suhwagije-decoction (SGT) pharmacopuncture solution on the liver and spleen cells of aged rats. Methods : The liver and spleen cells of 10, 52, and 72 week old Sprague Dawley (SD) rats were divided into three groups: distilled water (DW), Vit.C, and SGT groups, which were treated with DW, Vit.C and SGT pharmacopuncture solution respectively. The levels of superoxide dismutase (SOD), glutathione (GSH) and nitric oxide (NO) were then measured. Results : 1. Treatment of SGT pharmacopuncture solution significantly decreased NO concentration in spleen cells of 52 week old rats as compared with that of other groups. 2. Treatment of SGT pharmacopuncture solution significantly increased SOD activity in spleen cells of 72 week old rats as compared with that of other groups. Conclusions : Suhwagije-decoction (SGT) pharmacopuncture solution may have an anti-oxidative effect in the spleen cells of aged rats.