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Production of Gastrodia elata Tuber using Armillaria spp. (Armillaria 속균을 이용한 천마의 생산)

  • Sung, Jae-Mo;Jung, Bum-Shig;Yang, Keun-Joo;Lee, Hyun-Kyung;Harrington, T.C.
    • The Korean Journal of Mycology
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    • v.23 no.1 s.72
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    • pp.61-70
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    • 1995
  • The genus Armillaria is important because they produce Gastrodia tubers. Seventy two isolates of Armillaria were obtained from fruit bodies grown on decayed wood in Korea. Twenty four isolates from Pinus koraiensis were identified as A. ostoyae. Two isolates from G. elata growing in the field were identified as A. mellea. Seven isolates from Acer ginnala and Quercus spp. were identified as A. tabescens. Thirty nine isolates were identified as A. gallica. Armillaria gallica was isolated from Quercus spp., Ainus japonica, Vitis amurensis and Prunus sargentii. Armillaria spp. isolates were divided into four groups based on the cultural characteristics. Group II (A. gallica KNU-A110) was better than the other groups for mycelial growth and rhizomorph formation. Isolate KNU-A110 proved to be good for production of G. elata tubers. This fungus forms mycelial fan in the plant tissue and rhizomorphs in contact with G. elata tubers. Gastrodia spp. was found in thirteen sites in Kangweon province in Korea. The plants were divided into three different kinds based on stem color. Plants with stems of brownish orange and greyish yellow were identified as G. elata, and those with greyish green colored stems were identified as G. gracilis. Gastrodia was collected mainly from humus soils rich in leaf debris, and slopes facing south from mid-May to mid-July. Once the new tubers are formed from the ancestry tuber, the ancestry tuber begins to decay. The offspring tuber, apparently gaining nutrients through rhizomorphs, begins to grow in length and slowly to enlarge. It takes three years for the offspring tuber to become ancestry tuber.

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Effect of Turbidity Changes on Tissues of Zacco koreanus (탁도 변화가 참갈겨니 (Zacco koreanus) 조직에 미치는 영향)

  • Shin, Myung-Ja;Kim, Jeong-Sook;Hwang, Yun-Hee;Lee, Jong-Eun;Seo, Eul-Won
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.73-80
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    • 2008
  • Present study aims to investigate the effect of muddy water on the gill and the kidney tissues of Zacco koreanus under high turbidity rearing condition. The gill of Z. koreanus showed abnormal shapes in its secondary lamellae and a rough surface with impure debris in the high level of turbidity and the longer raising period condition. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. In case of kidney tissue, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was wider. The SOD activities in both gill and kidney tissues were increased in proportion to the high level of turbidity. On the while, CAT and GPX activities were shown constant level in the gill, but were increased in the kidney in the high turbid muddy water. These results indicate that the harmful radicals which generate by high level of turbidity could be removed partly by antioxidant enzymes in the kidney. The concentrations of micro heavy metal ions accumulated in the gill increased drastically at the 1,000 NTU. Based on the above results, it is considered that the exposure to the high level of turbidity for long period may affect on the structures of tissues, and change the enzymatic balance in Z. koreanus, causing the fatal disease.

TISSUE LEVELS OF INTERLEUKIN-1α, INTERLEUKIN-1β AND TUMOR NECROSIS FACTOR-α IN PULPAL AND PERIAPICAL PATHOSIS (치수 및 치근단병소에서 interleukin-1α, interleukin-1β, tumor necrosis factor-α의 분포에 관한 연구)

  • Ko, Hyun-Jung;Chung, Kwan-Hee;Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
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    • v.23 no.1
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    • pp.316-327
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    • 1998
  • This study was designed to examine the tissue levels of interleukin-$1{\alpha}$(IL-$1{\alpha}$), interleukin-$1{\beta}$(IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) in inflamed human dental pulps and periapical lesions, and to determine the relationship between each cytokine and pulpal and periapical pathosis. The pulps used in this experiment, were obtained in routine endodontic treatment and the periapical lesions in periapical surgery after clinical diagnoses were performed. These specimens were divided into four groups as normal pulp group(control group, n=9), acute pulpitis group(n=g), chronic pulpitis group(n= 10) and periapical lesion group(n= 18) and stored in liquid N2. For extract preparation, tissues were finely minced with a scalpel, and the fragments were incubated in $0.5m\ell$ homogenizing buffer (0.1 mol/L potassium chloride, 0.02 mol/L TRIS; pH=7.6) for two hours and grinded with glass homogenizer. Debris was removed by centrifugation and supernatants were immediately tested with enzymelinked immunosorbent assay (ELISA, R&D Co., Minneapolis, USA). Following results were obtained; 1. The concentrations of IL-$1{\alpha}$ in all experimental groups were significantly higher than in control group(p<0.05). And the concentrations of IL-$1{\alpha}$ in periapical lesion group were somewhat higher than in two pulpitis groups, but the differences among those groups were not stastically significant (p>0.05). 2. The concentrations of IL-$1{\beta}$ in all experimental groups were significantly higher than in control group (p<0.05), and all the experimental groups expressed similar concentrations. 3. The concentrations of TNF-${\alpha}$ in all experimental groups were higher than in control group but only the differences between chronic pulpitis group and control group were statistically significant(p<0.05). And the concentrations of TNF-${\alpha}$ in acute and chronic pulpit is groups were higher than in periapical lesion group but only the differences between chronic pulpitis group and periapical lesion group were statistically significant (p<0.05). 4. There was significant correlation only between IL-$1{\alpha}$ and IL-$1{\beta}$ in periapical lesion group (p<0.05).

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Arthroscopic Reduction and Fixation of an Anterior Cruciate Ligament Avulsion Fracture From the Tibial Eminence Using Bioabsorbable Pins - Technical Note - (생흡수성 핀을 이용한 소아 경골 과간부 견열 골절의 관절경적 정복 및 고정 방법 - 수술 술기 -)

  • Lee, Su-Chan;Yang, Il-Soon;Seo, Hee-Soo
    • Journal of the Korean Arthroscopy Society
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    • v.13 no.2
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    • pp.183-187
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    • 2009
  • Purpose: We describe a new and simple technique for arthroscopic fixation of tibial intercondylar eminence avulsion fracture using bioabsorbable pins in skeletally immature patients. Operative Technique: Diagnostic knee arthroscopy is performed using anterolateral and anteromedial portals. Fracture debris and blood clot are debrided to expose the injured site well. The fragment is reduced with the probe and fixed temporarily with a 1.1-mm diameter K-wire that is inserted percutaneously from the anterosuperior aspect of the knee joint. The drill guide is introduced into the joint and the fragment is secured by bioabsorbable, poly-p-dioxanone 1.3-mm pins inserted from different angles. The pins are 40 mm in length. The knee is placed in a long leg cast in extension for 4 weeks to assure that full extension is obtained. Conclusion: Arthroscopic fixation of an tibial intercondylar eminence avulsion fracture using bioabsorbable pins is not a technically demanding, suitable method that ensures fracture healing and restores the stability of the joint.

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Studies on Countermeasures for Preventing Loss of Human Life Caused by Landslides (산사태(山沙汰)로 인한 인명재해(人命災害) 예방대책(豫防對策)에 관(關)한 연구(硏究))

  • Woo, Bo Myeong
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.228-241
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    • 1989
  • The objectives of this study are to identify flood disasters resulted from heavy rainstorm including earth and stone-debris avalanches and also to develope the scientific data to be needed for establishing the landslide-related disaster prevention countermeasures. For this study, 5 Gun (district) regions including Booyeo, Seochun, Gongju, Boryung, and Chungyang in Chungchongnam-do of the central part of Korea, in which severe landslide damages have been triggered during 3 days from July 21 to July 23, 1987, were investigated. Mostly, landslides having death of human lives triggered from 6 a.m, to 8 a,m. on July 22, and the principal factor was proved to be the continuous heavy rain ; the continuous rainfall of internal region for 3 days measured about 300-673 mm. The structural measures for slope failure prevention countermeasures at the hollow part of upper hillslope should be required. Natural drainage network on slopes should not be disturbed in case of land use alteration, such as a chestnut planting work on hillslopes behind the houses particularly. There are so many problems in recognition of landslide disaster prevention countermeasures including evacuation exercises. More actual education of countermeasures for control of flood and landslide should be put to practice through "civil defense education" and "inhabitants' meeting." In this context, existing Erosion Control Stations of 13 regions established in each Province should not be reduced. The designation criterion and surveying processes of "Landslide Prone Site" published by Forest Administration should also be improved scientifically.

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Analysis and Improvement for Manual to Protect Mountain Disaster in Urban Area (도심지 토사재해 예방을 위한 기존 매뉴얼 분석과 개선 방안)

  • Song, Byungwoong;Baek, Woohyun;Yoon, Junghwan;Sim, Oubae
    • Journal of the Korean GEO-environmental Society
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    • v.16 no.7
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    • pp.43-53
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    • 2015
  • More than 70 percent of the Korean territory consists of mountain area so development of mountain district is essential to urbanize continuously. Thus, technological developments for risk factors and standards and manuals must be needed to prevent mountain disaster. Risk Management Manual should be made and operated in government legislation related to national disaster, but there is still no Emergency Management Standard Manual and Emergency Response-Practical Manual to prevent mountain disaster. This study suggests the improvement plans that are legislated but not established cleary in the field of disaster in urban area. The main items are like as 1) adaptable standard and practical manual to prevent mountain disaster in urban area, 2) reinforcement between managing department and interagency vertically and horizontally in central and local government organization, 3) Personal SOP (Standard Operating Procedure) not EOP (Emergency Operation Plan), 4) considering 13 items selected by Ministry of Public Safety and Security, 5) schematization with personal action plan, 6) check list to do in the event of mountain disaster, and 7) regular practice per quarter.

Experimental study on debris reduction facilities verification for small bridge and culvert (소교량 및 암거 대상 유송잡물 저감시설 검증실험)

  • Kim, Jong Tae;Yeo, Hong Koo;Kang, Jun Gu;Kim, Sung Joong
    • Proceedings of the Korea Water Resources Association Conference
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    • 2016.05a
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    • pp.447-447
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    • 2016
  • 홍수 시 하천을 따라 유하되는 유송잡물(잡목, 생활쓰레기 등)은 구조물에 집적이 이루어지며 이로 인해 물의 흐름을 방해하고 구조물 주변의 지반을 약화시키거나 월류로 인해 심각한 피해를 야기 시킨다. 교량의 경우 유송잡물의 집적은 교각의 항력을 증가시켜 전도파괴를 유발시킬 수 있으며, 교각주변 흐름교란을 통한 하상세굴로 인해 기초부를 파괴시키기도 한다. 또한 통수단면적 증가로 인해 높아진 수위는 제방을 월류시켜 재산 및 인명피해를 유발하기도 한다. 암거의 경우 유송잡물을 포함한 토사로 인해 단면폐색 및 침식으로 암거의 파괴를 발생시키고 이로인해 도로의 단절을 가져오기도 한다. 이러한 유송잡물에 대한 차단대책은 현재 까지 유지관리를 통한 방법만이 최선책으로 제시되고 있다. 본 연구는 국외에서 소개되고 있는 유송잡물 피해저감시설을 대상으로 축소모형실험을 통해 효과를 확인하고 이를 통해 저감시설의 효율에 대해 소개하고자 하는 것이 주목적이다. 유송잡물 저감시설은 교량의 경우 수직분리대 방법, 우회말뚝 방법, 스위퍼를 이용한 방법으로 소개하고 있으며 이들 방법의 특징은 유송잡물 우회를 통해 집적을 저감하는 방식이다. 암거의 경우 수직분리대 방법, 스크린 방법, 우회스크린을 이용하는 방법 등이 있으며 수직분리대는 교량과 마찬가지로 우회방식을 기본방식으로 스크린 방식은 유송잡물의 차단을 원칙으로 하고 있다. 실험의 결과는 교량과 암거의 차단시설 유무에 따른 유송잡물의 집적률을 대상으로 하였다. 차단시설이 없는 경우 교량에서의 집적률은 97.9% ~ 99.2%의 집적률로 나타났다. 수직분리대의 경우 유송잡물 집적률은 49.3%, 우회말뚝은 0.0% ~ 4.2%의 범위로 매우 높은 차단효과를 보이고 있었다. 스위퍼방식의 경우 집적률은 5.3% ~ 20.9%로 나타났다. 이러한 결과로 판단하였을 때 차단시설 설치로 인한 교량에 대한 직접적인 차단효과는 수직분리대 < 스위퍼 < 우회말뚝 순으로 높은 차단효과를 보이는 것으로 나타났다. 그러나 우회말뚝에서의 집적률이 한계치를 넘어선다면 유송잡물로 인해 흐름교란, 수위 및 하상변동, 말뚝의 유실 등으로 인한 피해가 예상된다. 암거대상 유송잡물 차단시설 실험결과는 수직분리대의 경우 type과 투하방법에 따라 차이가 있지만 집적률은 14.7% ~ 64.9%의 범위로 나타났다. 스크린과 우회스크린 방식은 유송잡물의 우회가 아닌 차단이 목적이므로 유송잡물 집적에 의한 수위상승이 우려되는바 수위영향을 검토하였다. 검토결과 스크린 설치로 인한 수위상승이 우회스크린보다 높게 상승하는 것으로 나타났다. 이러한 결과는 스크린의 형태적인 측면에서 2면을 통해 집적되는 특성을 갖는 우회스크린이 통수단면적을 많이 확보하기 때문이다. 따라서 국부적인 수위상승으로 인한 피해를 저감하기 위해서는 설치여건이 허용된다면 통수단면적 확보가 유리한 우회스크린 설치가 유리할 것으로 판단된다.

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Three-dimensional micro photomachining of polymer using DPSSL (Diode Pumped Solid State Laser) with 355 nm wavelength (355nm 파장의 DPSSL을 이용한 폴리머의 3차원 미세 형상 광가공기술)

  • 장원석;신보성;김재구;황경현
    • Korean Journal of Optics and Photonics
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    • v.14 no.3
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    • pp.312-320
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    • 2003
  • The basic mechanistic aspects of the interaction and practical considerations related to polymer ablation were briefly reviewed. Photochemical and photothermal effects, which highly depend on laser wavelength have close correlation with each other. In this study, multi-scanning laser ablation processing of polymer with a DPSS (Diode Pumped Solid State) 3rd harmonic Nd:YVO$_4$ laser (355 nm) was developed to fabricate a three-dimensional micro shape. Polymer fabrication using DPSSL has some advantages compared with the conventional polymer ablation process using KrF and ArF laser with 248 nm and 193 nm wavelength. These advantages include pumping efficiency and low maintenance cost. And this method also makes it possible to fabricate 2D patterns or 3D shapes rapidly and cheaply because CAD/CAM software and precision stages are used without complex projection mask techniques. Photomachinability of polymer is highly influenced by laser wavelength and by the polymer's own chemical structure. So the optical characteristics of polymers for a 355 nm laser source is investigated experimentally and theoretically. The photophysical and photochemical parameters such as laser fluence, focusing position, and ambient gas were considered to reduce the plume effect which re-deposits debris on the surface of substrate. These phenomena affect the surface roughness and even induce delamination around the ablation site. Thus, the process parameters were tuned to optimize for gaining precision surface shape and quality. This maskless direct photomachining technology using DPSSL could be expected to manufacture tile prototype of micro devices and molds for the laser-LIGA process.

A Performance Comparison of Machine Learning Classification Methods for Soil Creep Susceptibility Assessment (땅밀림 위험지 평가를 위한 기계학습 분류모델 비교)

  • Lee, Jeman;Seo, Jung Il;Lee, Jin-Ho;Im, Sangjun
    • Journal of Korean Society of Forest Science
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    • v.110 no.4
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    • pp.610-621
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    • 2021
  • The soil creep, primarily caused by earthquakes and torrential rainfall events, has widely occurred across the country. The Korea Forest Service attempted to quantify the soil creep susceptible areas using a discriminant value table to prevent or mitigate casualties and/or property damages in advance. With the advent of advanced computer technologies, machine learning-based classification models have been employed for managing mountainous disasters, such as landslides and debris flows. This study aims to quantify the soil creep susceptibility using several classifiers, namely the k-Nearest Neighbor (k-NN), Naive Bayes (NB), Random Forest (RF), and Support Vector Machine (SVM) models. To develop the classification models, we downscaled 292 data from 4,618 field survey data. About 70% of the selected data were used for training, with the remaining 30% used for model testing. The developed models have the classification accuracy of 0.727 for k-NN, 0.750 for NB, 0.807 for RF, and 0.750 for SVM against test datasets representing 30% of the total data. Furthermore, we estimated Cohen's Kappa index as 0.534, 0.580, 0.673, and 0.585, with AUC values of 0.872, 0.912, 0.943, and 0.834, respectively. The machine learning-based classifications for soil creep susceptibility were RF, NB, SVM, and k-NN in that order. Our findings indicate that the machine learning classifiers can provide valuable information in establishing and implementing natural disaster management plans in mountainous areas.

A Study on Detection and Monitoring in land creeping area by Using the UAV (무인기를 활용한 산지 땅밀림 피해지점 탐지 및 모니터링 방안 연구)

  • Seo, Jun-Pyo;Woo, Choong-Shik;Lee, Chang-Woo;Kim, Dong-Yeob
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.481-487
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    • 2018
  • This paper proposes a method to detect and monitor the land creeping area using a UAV to analyze the damaged area efficiently. Using a UAV, it was possible to secure the safety of the investigators before the field survey and effectively utilize it to establish an investigation plan because an orthophoto can be used to detect and scale the cracks in a land creeping area. In addition, it was possible to analyze the scale of the crack quantitatively by extracting the topographic information from the orthophoto. The study sites were found to have a total crack area of 1.01 ha, a length of 1.07 km, an average width of 10 m, and a step distance of 1 to 10 m. Periodic UAV measurements can be used to detect displacements on the land creeping area and monitor the direction and scale of crack spread. Therefore, it is expected to be used effectively during recovery planning. Applying the UAV to the land creeping area resulted in the qualitative and quantitative results quickly and easily in dangerous mountainous watersheds. Therefore, it is expected that it will contribute to the development of related industries because of the high availability of a UAV in forest soil sediment disasters, such as landslides, debris flow, and land creeping area.