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Regulation of the plasminogen activator activity and inflammatory environment via transforming growth factor-beta regulation of sperm in porcine uterine epithelial cells

  • Kim, Su-jin;Cheong, Hee-Tae;Park, Choon-keun
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.4
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    • pp.297-306
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    • 2020
  • The aims of the present study were to confirm that regulation of the PA and environment via TGF-β regulation of sperm by Percoll-separated in porcine uterine epithelial cells. And, it was performed to identify the cytokines (TGF-β1, 2 and 3, TGF-β receptor1 and 2; interleukin, IL-6, IL-8) and PA-related genes (urokinase-PA, uPA; tissue-PA, tPA; PA inhibitor, PAI; uPA-receptor, uPAR) by spermatozoa. The experiment used porcine uterus epithelial cells (pUECs) and uterine tissue epithelial cells, Boar sperm were separated by discontinuous Percoll density gradient (45/90%), and tissues were co-incubated with spermatozoa, followed by real-time PCR. PA activity was measured of sperm by discontinuous Percoll density gradient (45/90%) for 24 hours. To measure viability and acrosome damage of sperm double stained propidium iodide (PI) and SYBR-14 or FITC-PNA were used. In results, binding ratio of Percoll-separated sperm was found no differences, but sperms isolated from 90% Percoll layer reduced PA activity (p < 0.05). when co-cultured sperm selected Percoll in porcine uterus tissues epithelial cells, 90% layer sperm increased TGF-β R1, contrastively tPA and PAI-1 in comparison with control (p < 0.05). 45% sperm was decreased the expression of uPA (p < 0.05). TGF-β decreased PA activity in the supernatant collected from pUECs (p < 0.05). Especially, The group including uPA, PAI-1 were induce sperm intact, while it was reduced in sperm damage when compared to control (p < 0.05). Also, there was no significant difference group of tPA and tPA+I in the dead sperm and acrosome damage compared to control. The expression of tPA and PAI showed a common response. Percoll-separated spermatozoa in 90% layer reduced tPA and IL-related gene mRNA expression. Thus, Percoll-sparated sperm in 90% layer show that it can suppress inflammation through increased expression of TGF-β and downregulation of PA and IL in epithelial cells compared to 45% layer Percoll.

The Succession and Method of Internal Alchemy of Five-Masters in Southern School Taoism (도교 남종 5조의 전승과 내단법)

  • Kim, Kyeong-soo
    • The Journal of Korean Philosophical History
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    • no.39
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    • pp.143-168
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    • 2013
  • The purpose of this paper is to study the succession and method of five-masters of southern school in transmitting the internal alchemy in Taoism which is established by Zhang Bo-duan. In history, the internal alchemy is divided into a few schools but all of them are under the umbrella of Zhang Bo-duan's alchemy. The five master's method has been consistent from Zhang Bo-duan to Seok Tae to Seol Do Gwang based on three reasons. First, they lived long while practicing the method. Second, they learned the method from their direct master secretly. Third, they kept practicing the individual method while staying in the secular world rather than in a religious body. I limit three of them Zhang Bo-Dan, Seok Tae, Seol Do Gwang as figures who practiced the pure internal alchemy. Thus I argue that the method stayed only for 200 years, arising from the late Dang Dynasty and disappearing in 1911, when Seol Do-Gwang was dead. This method can be instructed only to the appropriate figure, and the specific steps of method are taught through the oral instruction so that this method cannot be wide spread. Based on the fact that the time of practicing method is quite long and it needs intensive and constant efforts, it is difficult for a mundane person to practice and cope with the training. Jin Nam and Baek Ok Seom are believed that they belonged to the school of Shin So. Baek Ok Seom is regarded as a figure that made efforts to unite the school of Wei and the internal alchemy. However, he made lots of fabrication and discrepancy. Thus I state that Jin Nam and Baek Ok Seom need to be out in the succession and method of five masters of southern school in Taoism. Their changed method in quality needs to be organized in a new genealogy.

Patent Analysis on Fuel Cell By-Product Utilization Technology for Operating Expenditure Reduction of Hydrogen Residential Buildings (수소에너지 주거건물의 운영비용 감축을 위한 연료전지 발전 부산물 활용기술에 관한 특허분석)

  • Ji, SangHoon;Kim, WeonJae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.12
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    • pp.488-493
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    • 2020
  • The demand for hydrogen, which is considered an environmentally friendly energy source, is increasing, and at the same time, the fuel cell market is increasing continuously. This study aimed to establish a strategy for securing intellectual property rights on fuel cell by-product utilization technology for operating expenditure reduction of hydrogen-powered residential buildings. In this patent analysis, this study investigated Korean, American, Japanese, and European patents filed/published/registered by October 2019 and established a technical classification system and classification criteria through expert discussion. To reduce the operating expenditure of hydrogen-powered residential buildings, intellectual property rights will be improved using systems and methodologies involving cathode-side purified air, product water, and oxygen-depleted air captured with the dead-end mode operation of polymer electrolyte fuel cells.

LCL Cargo Loading Algorithm Considering Cargo Characteristics and Load Space (화물의 특성 및 적재 공간을 고려한 LCL 화물 적재 알고리즘)

  • Daesan Park;Sangmin Jo;Dongyun Park;Yongjae Lee;Dohee Kim;Hyerim Bae
    • Journal of Intelligence and Information Systems
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    • v.29 no.4
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    • pp.375-393
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    • 2023
  • The demand for Less than Container Load (LCL) has been on the rise due to the growing need for various small-scale production items and the expansion of the e-commerce market. Consequently, more companies in the International Freight Forwarder are now handling LCL. Given the variety in cargo sizes and the diverse interests of stakeholders, there's a growing need for a container loading algorithm that optimizes space efficiency. However, due to the nature of the current situation in which a cargo loading plan is established in advance and delivered to the Container Freight Station (CFS), there is a limitation that variables that can be identified at industrial sites cannot be reflected in the loading plan. Therefore, this study proposes a container loading methodology that makes it easy to modify the loading plan at industrial sites. By allowing the characteristics of cargo and the status of the container to be considered, the requirements of the industrial site were reflected, and the three-dimensional space was manipulated into a two-dimensional planar layer to establish a loading plan to reduce time complexity. Through the methodology presented in this study, it is possible to increase the consistency of the quality of the container loading methodology and contribute to the automation of the loading plan.

Current Situation on Signing Advance Medical Directives and Actual Life-sustaining Treatment Given at a University Hospital (일개 대학병원의 연명치료 선택 및 사전의료의향서 작성 현황)

  • Yoon, Ho-Min;Choi, Youn-Seon;Hyun, Jong-Jin
    • Journal of Hospice and Palliative Care
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    • v.14 no.2
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    • pp.91-100
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    • 2011
  • Purpose: This study was performed to investigate patients' preferences on receiving life-sustaining treatments (LST) and to analyze the relationship between patients' characteristics and LST selection. We also examined any discrepancy between LST patients' choices regarding medical intervention and actual medical intervention given/not given within 48 hours before death. Methods: This cross-sectional study was performed from March 1, 2008 to August 31, 2008 in the Palliative Care Unit of Korea University Hospital. Electric medical records (EMR) of 102 hospice cancer patients were reviewed, and 74 patients with Glasgow coma scale (GCS) ${\geq}$10 at the time of signing the advance medical directives (AMD) were selected for the first analysis. Then, patients alive at the end of this study, transferred to other hospitals or dead within 48 hours were excluded, and the remaining 42 patients were selected for the second analysis. Results: Preferred LST included antibiotics, total parenteral nutrition, tube feeding, transfusion, and laboratory and imaging studies. The relationship between patients' characteristics and LST could not be analyzed due to skewed preferences. LST chosen at the time of signing the AMD and actual medical intervention given/not given in the last 48 hours showed discrepancy in most cases. Conclusion: When making AMD in hospice cancer patients, it is important to consider the time and possibility of changing the choices. Above all, patients must fully understand the AMD. Thus, LST should always be provided with careful consideration of all possibilities, because legal and social aspects of AMD have not been established yet.

Monitoring of Pulmonary Tuberculosis by Polymerase Chain Reaction After Antituberculous Treatment (항결핵제 투여후 중합효소연쇄반응으로 추적한 폐결핵 환자들의 치료반응 관찰)

  • Jeon, Chang-Ho;Suh, Hun-Suk;Lee, Sang-Chae;Hyun, Dae-Sung;Ahn, Wook-Su
    • Tuberculosis and Respiratory Diseases
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    • v.45 no.5
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    • pp.935-941
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    • 1998
  • Background: As living and dead Mycobacteria could be amplified by polymerase chain reaction(PCR), it was considered that PCR was inappropriate for the monitoring of pulmonary tuberculosis after treatment. But we found negative conversion of PCR after successful treatment. We would like to know about the negative conversion rate of PCR and its conversion time after antituberculous treatment. Methods: We collected 113 sputums from the 16 patients of pulmonary tuberculsosis visiting Catholic University Hospital of Taegu Hyosung. We consecutively tested AFB smear, AFB culture and PCR by 2 to 4 weeks after antituberculous therapy. The patients were classified according to the chest X ray findings. Results: We detected negative conversion of PCR from all 16 patients of the pulmonary tuberculosis within 30 weeks after treatment. The average negative conversion time was $16{\pm}8$ weeks. The conversion time according to the chest X -ray findings were as follows : For the 8 cases of minimum were $9{\pm}5$ weeks, 4 cases of modreate advanced were $20{\pm}8$ weeks, and 4 cases of far advanced were $23{\pm}2$ weeks. The product of PCR was gradually decreased according to the duration of treatment. Conclusions: From the results of our study, we could utilize M. tubercuosis PCR for the prediction of therapy response and monitoring of the patient with pulmonary tuberculosis after treatment.

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On the Growth, Feeding Rates and the Efficiency of Food Conversion for Cuttlefishes and Squids (오징어, 꼴뚜기류의 성장 및 섭이율과 이료의 전환효율)

  • CHOE, Sang
    • The Korean Journal of Zoology
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    • v.9 no.2
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    • pp.12-20
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    • 1966
  • Studies on the rate of growth, the rate of feeding and the efficiency of food conversion on the stage of new-born fries to the near adult size for three species of cuttlefishes , Sepia esculenta, Sepia subaculeata, Sepiella maindroni and two species of squids, Sepioteuthis lessomiana, Euprymna berryi were carried out in the process of artificial raising, and then argued about a feasibility of the propagation of cuttlefishes and squids. 1. The relation between the daily age (D) and the body weight(W) of Sepia exculent is expressed in a logarithmic equation, log W=3.0649 log D-4.2768. The daily rates of growth through 121 days of the raising period were 1.46 per cent for the man시 length and 1.67 percent for the body weight. The raipidest growth of Sepia esculenta is observed at the stage of 1 to 4 cm in the mantle length . At that time the daily rates of growth reach 3.3 to 5.5 percent for the mantle length and 10.4 to 12.0 percent for the body weight, respectively. The growth of Sepia esculenta varies a great deal to the bait. When fed on a dead bait the rates of growth decrease 17 per cent for the mantle length and 26 percent for the body weight compared with those fed on a live bait. 2. The relation between the daily age and the body weight of Sepia subaculeata is expressed in a logarithmic equation, log W=3.7447 log D-4.9003. The daily rates of growth through 110 days of the raising period were 1.63 percent for the mantle length and 1.83 percent for the body weight. The rapidest growth of Selia subaculeata is observed at the stage of 1.5 to 9.0 cm in the mantle length. At that time the daily rates of growth reach 3.1 to 7.4 percent for the mantle length and 6.8 to 16.7 percent for the body weight , respectively. 3. The relation between tehdaily age and the body weight of Sepiella maindroni is expressed in a ogarithmic equation , log W=2.9332 log D-3.8224 . The daily rates of growth through 133 days of the rearing period were 1.39 percent for the mantle length and 1.51 percent for the body weight . The rapidest growth of Sepiella maindroni is observed at the stage of 0.4 to 5.8 cm in the mantle length. At that time the daily rates for growth reach 4.6 to 7.3 percent for the mantle length and 8.5 to 15.4 percent for the body weight , respectively. 4. The daily rates of growth onthe stage of 0.5 to 6.0 comin the mantle length of Sepioteuthis lessoniana were 4.1 to 5.9 percent for the mantle length and 7.1 to 10.7 percent for the body weight . 5. During the rearing period of 31 days immediately after the hatching , the daily rateof feeding of Sepia esculenta marked 11.0 to 39.4 percent (28.2 percent in an average), and the efficiency of food conversion of this species reached 9.0 to 71.0percent (38.7percent in an average). Even at the more growing stage of 4.5to 6.2 cm in the mantle length, the daily rate of feeding of three species of cuttlefishes wee maintained 17.7 percent for Sepia esculenta, 30.8 percent for Sepia subaculeata and 34.7percent for Sepiella maindroni on an average. 6. The efficiency of food coversion of cuttlefishes and squids are larger than those of other fishes, and all the species are rapid in their growth. Four to five months are thought to be enough for their growing into a fair commercial size.

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The Attitude of the Bereaved Family Attending a Bereavement Memorial Service (사별가족모임과 관련된 사별가족 태도 연구)

  • Jung, In-Soon;Shim, Byoung-Yong;Kim, Young-Seon;Lee, Ok-Kyung;Han, Sun-Ae;Shin, Ju-Hyun;Lee, Jong-Ku;Hwang, Su-Hyun;Ok, Jong-Sun;Kim, Hoon-Kyo
    • Journal of Hospice and Palliative Care
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    • v.8 no.2
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    • pp.143-151
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    • 2005
  • Purpose: Bereavement Memorial Service has been held every year by the hospice team at St. Vincent's Hospital for the purpose of supporting the bereaved family who feel grief and mourning. The purpose of this study is to find out the attitude of the bereaved attending at bereavement memorial service (BMS) and to find out the areas needing improvements to set up better memorial service. Methods: Hospice team sent invitation card to 180 families of patients who admitted and passed away at hospice ward Nov., 2003${\sim}$Oct., 2004. Among them 22 families attended the BMS meeting, which was held on 26th Nov., 2004. The researcher collected data from 22 families with 'Questionnaire' survey. Except identifying data and 2 dichotomy questions, we used open-ended questionnaire. 1 researcher conducted a telephone interview survey in 18 families who couldn't attend at BMS meeting. Results: The median age was 56 (range $16{\sim}19$) and there were 37 females and 3 males. They were patient's wife (22), mother (4), husband (5), daughter (4), mother-in-law (1), siblings (1), brothers wife (1). Duration after bereavement, $1{\sim}3$ months (17) was the highest frequency. 36 families agreed 'the dead experienced the death with dignity'. The reason of agreement to the death with dignity was 'the patient died in preparation' (16). 'the patient died in well-being condition spiritually' (9), 'the patient died in comfort physically (7). 4. persons thought the dead died with indignity. The bereaved defined 'the death with dignity' as follows: 'acceptance of death & death in spiritual well-being' (9), 'death in physical comfort condition' (7), 'the death in psycho-social well-being' (3), non-respondents (10). Most families (21) were still in difficulty to overcome bereavement grief. The answer regarding the method to overcome the difficulty was 'with spiritual sublimation' (13), 'with devotion of oneself in daily life' (10), 'with devotion to mourning as it is' (3). With regard to their attitude to invitation, 'having joy and thanks from hospice team' (21), 'grief' (4), 'suffering' (4). Toward the existence of hesitation about attendance at BMS meeting, the result as follows. Nonexistence of hesitation respondent (34), existence respondent (6), the reason for hesitation was various; 'the meeting reminds me of the suffering times', 'the meeting makes me to recall, and it will be likely to cry', and so on. The needs and feelings to memorial service meeting were various; 'it was meaningful time', 'it was good to recall about the dead', 'more meeting annually' and so on. In respect of the most difficulty after bereavement, in attendant family, 'depression' (10) was the highest frequency, whereas, in non-attendant family, the most difficult thing was 'financial problem/role difficulty (6). Conclusion: This study shows the rate of attendance was high in bereaved whose bereavement duration $1{\sim}3$ month. Most of bereaved were still suffering from bereavement grief within 1 year. Although most families didn't hesitate and felt positive mood to invitation, the rate of attendance was low. Comparing with two groups between attendant family and non-attendant, the latter felt more difficulty in 'financial problem/role difficulty, on the other hand, the former felt difficulty in 'depression'. Hereafter, the additional study about the factor relating to these attitude and needs of the bereaved relating to memorial service will be necessary.

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Cryptocaryoniasis of cultured flounder, Paralichthys olivaceus in low temperatures (저수온 양식 넙치 Paralichthys olivaceus의 Cryptocaryoniasis)

  • Ji, Bo-Young;Kim, Ki-Hong;Park, Soo-Il
    • Journal of fish pathology
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    • v.10 no.2
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    • pp.97-111
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    • 1997
  • In the winter of 1995, mass mortality occurred in cultured flounder, Paralichthys olivaceus in Gurongpo, Kyoungbuk, Korea. From the observations of moribund and dead fish, parasitic ciliates, which were shown as white spots to the naked eye, were considered to be involved in the mass mortality. From heavily infected flounders, histopathological, morphological and biological characterization of these ciliates were carried out. In the histological observation, many ciliates were found under the epithelia of gill filaments and skin, and caused hyperplasia of epithelial and mucus cells at the infected areas. The ciliates found on the body surface, fins and gills were very similar to Cryptocaryon irritans. However the ciliates showed two different patterns of reproductian, i.e., typical form(palintomy)and atypical form(budding plus multiple fission) at $16^{\circ}C$ of water temperature. The occurrence ratio between typical and atypical form was about 3:2. Tomitogenesis takes 8-14 days in the typical and 13-15 days in the atypical form. In the viability test at different temperatures and salinities, the typical form died below 30‰ at $12^{\circ}C$, below 20‰ at $16^{\circ}C$, below 15‰ at $20^{\circ}C$, and below 25‰ at $24^{\circ}C$, respectively. On the other hand, the atypical form died below 20‰ at $12^{\circ}C$, below 15‰ at 16-$20^{\circ}C$, and below 25‰ at $24^{\circ}C$, respectively. The results suggested that the atypical has better viability at low salinity than that of the typical at low temperatures. In the excystment time and success rates of excystment according to temperatures, the typical form showed 8 days, 30% at $12^{\circ}C$ : 6.5 days, 50%, at $16^{\circ}C$ : 5.5 days, 75% at $20^{\circ}C$ : and 7 days, 10% at $24^{\circ}C$, respectively. On the other hand, the atypical form showed 15.5 days at $12^{\circ}C$ : 14 days, 76.6% at $16^{\circ}C$ : 12 days, 72.2% at $20^{\circ}C$ : 10 days 31.6% at $24^{\circ}C$, respectively. The results suggested that the atypical form had longer excystment time than that of the typical form at any temperature and showed better stability at low temperatures.

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Effect of Oregano (Origanum vulgare L.) on the Survival of Escherichia coli ) O157:H7 and Staphylococcus aureus 196E during Cold Storage (저온저장중 Oregano(Origanum vulgare L.)가 Eschetichia coli O157:H7과 Staphylococcus aureus 196E의 생존에 미치는 영향)

  • 박찬성;박금순;김미림
    • Korean journal of food and cookery science
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    • v.13 no.4
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    • pp.440-447
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    • 1997
  • The antibacterial effect of low concentrations of oregano (Origanum vulgare L.) in culture broth against Escherichia coli O157:H7 and Staphylococcus aureus 196E was tested at 35,5 and -20$^{\circ}C$. Tryptic soy broth (TSB) containing 0∼2% (w/v) of oregano was inoculated with 10$\^$6/∼10$\^$7/ CFU/$m\ell$ of E. coli or S. aureus and incubated at each temperature. The growth of E. coli was not inhibited at 0.1∼1.0% oregano and the growth occured at 2% oregano but only after a prolonged lag period. The death rate of E. coli after stationary phase was increased with increasing concentration of oregano in culture broth. The growth of S. aureus was inhibited with increasing concentration of oregano at 35$^{\circ}C$. Growth of S. aureus occured at the presence of 0.3∼0.5% oregano after a long lag period while the viability at 1.0∼2.0% was decreased during storage at 35$^{\circ}C$. During refrigerated storage at 5$^{\circ}C$, inhibition of E. coli or S. aureus was increased with the progress of time and increasing spice concentration. At the presence of 0.5∼2.0% oregano, E. coli and S. aureus were dead after 20 and 16 days of refrigerated storage, respectively. During frozen storage at -20$^{\circ}C$, the antibacterial activity of oregano against E. coli was increased with increasing storage time and spice concentration while the antibacterial activity against S. aureus was effective during the early period of storage, and no changes in the population of S. aureus occurred at different concentrations of oregano during frozen storage. Viable counts of E. coli were 1/3∼l/7 and S. aureus were 1/18∼l/46 of the control at 0.1% oregano in culture broth during frozen storage.

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