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Assessing the association of single nucleotide polymorphisms in thyroglobulin gene with age of puberty in bulls

  • Fernandez, Maria Elena;Goszczynski, Daniel Estanislao;Prando, Alberto Jose;Peral-Garcia, Pilar;Baldo, Andres;Giovambattista, Guillermo;Liron, Juan Pedro
    • Journal of Animal Science and Technology
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    • v.56 no.5
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    • pp.17.1-17.4
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    • 2014
  • Puberty is a stage of sexual development determined by the interaction of many loci and environmental factors. Identification of genes contributing to genetic variation in this character can assist with selection for early pubertal bulls, improving genetic progress in livestock breeding. Thyroid hormones play an important role in sexual development and spermatogenic function. The objective of this study was to evaluate the association between single nucleotide polymorphisms (SNPs) located in thyroglobulin(TG) gene with age of puberty in Angus bulls. Four SNPs were genotyped in 273 animals using SEQUENOM technology and the association between markers and puberty age was analyzed. Results showed a significant association (P < 0.05) between these markers and puberty age estimated at a sperm concentration of 50 million and a progressive motility of 10%. This is the first report of an association of TG polymorphisms with age of puberty in bulls, and results suggest the importance of thyroidal regulation in bovine sexual development and arrival to puberty.

High Temperature Behavior of Oxidized Mild Steel in Dry and Wet Atmospheres

  • Favergeon, J.;Makni, A.;Moulin, G.;Berger, P.;Lahoche, L.;Viennot, M.
    • Corrosion Science and Technology
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    • v.7 no.4
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    • pp.224-232
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    • 2008
  • During the hot rolling process, steels develop an oxide scale on their surface. This scale can affect the mechanical properties of the rolled steel and its surface aspect. The main problem comes from the mechanical integrity of the oxide scales which could delaminate or crack, leading eventually to later oxide incrustation within the steel. The objective of the present work is to qualify the mechanical integrity of the iron oxide scales during the hot rolling process. The laboratory experiments use a four point bending test to simulate the mechanical solicitation which takes place during the rolling sequence of the steel slabs. The oxide scales grow on a mild steel at $900^{\circ}C$ under wet or dry atmosphere and the oxidized steel is then mecahnically tested at $900^{\circ}C$ or $700^{\circ}C$. The high temperature four point bending tests are completed with microstructural observations and with the record of acoustic emission to follow in-situ the mechanical damages of the oxide scales. The results show the role of water vapor which promotes the scale adherence, and the role of the temperature as the oxide are more damaged at $700^{\circ}C$ than at $900^{\circ}C$.

L'etude du Costume Liturgique (그리스도교의 전례복에 관한 연구)

  • 오춘자
    • Journal of the Korean Home Economics Association
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    • v.12 no.34
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    • pp.743-769
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    • 1974
  • Au debut I'habit Iiturgique ne differait de I'habit de fete du simple citoyen que par sa richesse. C'est seulement quand, dans la vie courante, on adopta la robe courte que le vetememt liturgique commenca a se distinguer, meme par la forme, du costume civil. Les ornements liturgiques, en effet, ne sont qu'une forme stylisee de l'habit de fete de la fin de l'Empire Romain (du IIIeme au Veme siecle). Pour une ceremonie religieuse on se presente bien habille. Le meme sentiment de respect des choses sacrees a amene deja, vers la fin de l'antiquite chretienne, a donner au pretre un vetememt liturgique special. Pour celebrer la messe, le pretre revet par-dessus sa soutane un costume special, compose de l'amict, de l'aube, du cordon, du manipule, de l'etole et de la chasuble. Aux messes solennelles, les eveques ajoutent a ces ornements des bas et sandales, des gants, la tunique et la dalmatique avee la mitre ; en certain cas, les archeveques y joingnent le pallium. Aux messes solennelles, le diacre porte sur ; l'aube le manipule et la tunique. De ces pieces du costume liturgique, on dira brievement, apres leur emploi et leur forme actel, l'origine et l'histoire, mais seulement apres avoir marque les lignes generales parition et des transformations du costume liturgique dans son ensemble. L'evolution du costume liturgique a partir du XIIIeme siecle peut se resumer en quelques mots en raison souvent de la lourdeur des riches etoffes (velours et brocarts) et de l'importance donne a la decoration, toujours par rechereche d'une plus grande commodite on a abandonne lentement d'abord, puis rapidement adapte a partir du XVI eme siecle, les formes amples pour des formes courtes et etriquees. Il faut faire quelques exceptions qui marquent l'influence des gouts decoratifs de gouts epoque : La periode de 1700 a 1850 marque la complete decadence du costum liturgique. On a depuis essaye de lui rendre sa beaute et aussi sa signification symbolique, par un retour aux etoffes et a la decoration de la deuxieme partie du moyen age. Souhations que, sous la direction des liturgistes, en respectant l'essentiel de la liturgie aujourd'hui, avec le concours d'artistes epris du sens liturgique et des connaisseurs des tradtions et de toutes les ressources actuelles on trouve des costumes liturgiques adaptes a notre temps dans un style plus simple et correspondant au besoins actuels.

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Diversity of Paenibacillus spp. in the Rhizosphere of Four Sorghum(Sorghum bicolor) Cultivars Sown with Two Contrasting Levels of Nitrogen Fertilizer Assessed by rpoB-Based PCR-DGGE and Sequencing Analysis

  • Coelho, Marcia Reed Rodrigues;Mota, Fabio Faria Da;Carneiro, Newton Portilho;Marriel, Ivanildo Evodio;Paiva, Edilson;Rosado, Alexandre Soares;Seldin, Lucy
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.753-760
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    • 2007
  • The diversity of Paenibacillus species was assessed in the rhizospheres of four cultivars of sorghum sown in Cerrado soil amended with two levels of nitrogen fertilizer(12 and 120 kg/ha). Two cultivars(IS 5322-C and IS 6320) demanded the higher amount of nitrogen to grow, whereas the other two(FBS 8701-9 and IPA 1011) did not. Using the DNA extracted from the rhizospheres, a Paenibacillus-specific PCR system based on the RNA polymerase gene(rpoB) was chosen for the molecular analyses. The resulting PCR products were separated into community fingerprints by DGGE and the results showed a clear distinction between cultivars. In addition, clone libraries were generated from the rpoB fragments of two cultivars(IPA 1011 and IS 5322-C) using both fertilization conditions, and 318 selected clones were sequenced. Analyzed sequences were grouped into 14 Paenibacillus species. A greater diversity of Paenibacillus species was observed in cultivar IPA 1011 compared with cultivar IS 5322-C. Moreover, statistical analyses of the sequences showed that the bacterial diversity was more influenced by cultivar type than nitrogen fertilization, corroborating the DGGE results. Thus, the sorghum cultivar type was the overriding determinative factor that influenced the community structures of the Paenibacillus communities in the habitats investigated.

Bacterial and Fungal Communities in Bulk Soil and Rhizospheres of Aluminum-Tolerant and Aluminum-Sensitive Maize (Zea mays L.) Lines Cultivated in Unlimed and Limed Cerrado Soil

  • Mota, Da;Faria, Fabio;Gomes, Eliane Aparecida;Marriel, Ivanildo Evodio;Paiva, Edilson;Seldin, Lucy
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.805-814
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    • 2008
  • Liming of acidic soils can prevent aluminum toxicity and improve crop production. Some maize lines show aluminum (Al) tolerance, and exudation of organic acids by roots has been considered to represent an important mechanism involved in the tolerance. However, there is no information about the impact of liming on the structures of bacterial and fungal communities in Cerrado soil, nor if there are differences between the microbial communities from the rhizospheres of Al-tolerant and Al-sensitive maize lines. This study evaluated the effects of liming on the structure of bacterial and fungal communities in bulk soil and rhizospheres of Al-sensitive and Al-tolerant maize (Zea mays L.) lines cultivated in Cerrado soil by PCR-DGGE, 30 and 90 days after sowing. Bacterial fingerprints revealed that the bacterial communities from rhizospheres were more affected by aluminum stress in soil than by the maize line (Al-sensitive or Al-tolerant). Differences in bacterial communities were also observed over time (30 and 90 days after sowing), and these occurred mainly in the Actinobacteria. Conversely, fungal communities from the rhizosphere were weakly affected either by liming or by the rhizosphere, as observed from the DGGE profiles. Furthermore, only a few differences were observed in the DGGE profiles of the fungal populations during plant development when compared with bacterial communities. Cloning and sequencing of 16S rRNA gene fragments obtained from dominant DGGE bands detected in the bacterial profiles of the Cerrado bulk soil revealed that Actinomycetales and Rhizobiales were among the dominant ribotypes.

La época de la independencia de América latina y los roles de las mujeres (라틴아메리카 독립 시기와 여성의 역할)

  • LEE, Seong hun
    • Cross-Cultural Studies
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    • v.34
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    • pp.285-306
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    • 2014
  • La historia sobre la ${\acute{e}}poca$ de la independencia y la $formac{\acute{o}}n$ de las naciones latinoamericanas enfatiza sobre todo los roles de los hombres y $espor\acute{a}dicamente$ de las mujeres. Se han considerado directrices las acciones de algunos criollos de esa epoca como $Sim{\acute{o}}n$ Bolivar y San Martín. La historiografía masculina-$c{\acute{e}}ntrica$ $est{\acute{a}}$ ignorando y marginando las luchas y los sacrificios de las mujeres que participaron en la independencia y la $formac{\acute{o}}n$ de las naciones. Por tanto, es preciso realizar una crítica a los procesos que han mantenido la tendencia machista, uno de ellos es porque no se han desatendido concepciones y cosmovisiones sobre el $g{\acute{e}}nero$. En fin, esta tendencia es porque no se ha superado la herencia colonial de manera crítica. En este contexto, este artículo intenta investigar los cambios sobre los puntos de vista y la manera de ver a las mujeres y sus actividades durante la ${\acute{e}}poca$ de la independencia. Puesto que las mujeres han sido consideradas como objetos que ocupan un espacio secundario en $comparaci{\acute{o}}n$ a los roles de los criollos. Este estudio pretende configurar nuevos puntos de vista para reestructurar nuestro enfoque tradicional sobre la independencia y las formaciones de las naciones latinoamericanas para poner en evidencia los roles de las mujeres en la historia.

D$\acute{e}$veloppement d'un Programme d'$\acute{E}$cotourisme dans la R$\acute{e}$gion de Yeosu en Coree du Sud: le Cas de l'$\hat{I}$le de Sado et de son Relief Caract$\acute{e}$ristique (지형 특성에 기반한 여수시 사도일원의 생태관광 프로그램 구성)

  • Lee, Jeong-Hun
    • Journal of the Korean association of regional geographers
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    • v.17 no.6
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    • pp.738-752
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    • 2011
  • La c$\hat{o}$te m$\acute{e}$ridionale de la Cor$\acute{e}$e du sud, et principalement la ville de Yeosu et ses environs pr$\acute{e}$sentent un int$\acute{e}$r$\hat{e}$t particulier puisque le paysage offre de nombreuses presqu'$\hat{i}$les, baies et $\hat{i}$les. Pour tirer profit de ces paysages naturels il est d'abord n$\acute{e}$ssaire de les prot$\acute{e}$ger. Malgr$\acute{e}$ la haute valeur de ces paysages naturels, ils demeurent peu connus et les analyses $\acute{e}$cotouristiques de la ville de Yeosu et ses environs sont encore incompl$\grave{e}$tes. Par ailleurs, la protection de ces paysages naturels est rendue difficile par la d$\acute{e}$sagr$\acute{e}$gation d$\hat{u}$e aux sels halo$\ddot{i}$des. Cette recherche a pour objet l'$\acute{e}$tude du tourisme physico-$\acute{e}$cologique et sa contribution au d$\acute{e}$veloppement $\acute{e}$conomique d'une r$\acute{e}$gion de Yeosu en Cor$\acute{e}$e du sud. Nous nous int$\acute{e}$ressons particuli$\grave{e}$rement au d$\acute{e}$veloppement d'une route $\acute{e}$cotouristique, aux crit$\grave{e}$res de s$\acute{e}$lection du lieu $\acute{e}$cotouristique et $\grave{a}$ la pr$\acute{e}$sentation des explications touristiques, en tenant compte de l'$\hat{i}$le de Sado et de ses paysages naturels sur le plan de l'$\acute{e}$cotourisme. Il y a plusieurs ressources $\acute{e}$cotouristiques sur l'$\hat{i}$le de Sado et dans ses environs: la plage de sable et la falaise de l'$\hat{i}$le de Sado; les traces fossiles de dinosaures, la ripple-mark et la crevasse dans le sol boueux de l'$\hat{i}$le de Joungdo; le tombolo, l'affleurement tufac$\acute{e}$ et le dyke de l'$\hat{i}$le de Silouseom; le trou provoqu$\acute{e}$ par les sels halo$\ddot{i}$des et le dyke de l'$\hat{i}$le de Jangsado; la mer ass$\acute{e}$ch$\acute{e}$e entre l'$\hat{i}$le de Naquek et l'$\hat{i}$le de Choudo. On a, g$\acute{e}$n$\acute{e}$ralement, d$\acute{e}$velopp$\acute{e}$ les reliefs li$\acute{e}$s $\grave{a}$ la couche s$\acute{e}$dimentaire et les fossiles de la derni$\grave{e}$re p$\acute{e}$riode du m$\acute{e}$sozo$\ddot{i}$que. La route $\acute{e}$cotouristique part de l'embarcad$\grave{e}$re de l'$\hat{i}$le de Sado et continue du Nord jusqu'au Sud.

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Mutational Data Loading Routines for Human Genome Databases: the BRCA1 Case

  • Van Der Kroon, Matthijs;Ramirez, Ignacio Lereu;Levin, Ana M.;Pastor, Oscar;Brinkkemper, Sjaak
    • Journal of Computing Science and Engineering
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    • v.4 no.4
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    • pp.291-312
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    • 2010
  • The last decades a large amount of research has been done in the genomics domain which has and is generating terabytes, if not exabytes, of information stored globally in a very fragmented way. Different databases use different ways of storing the same data, resulting in undesired redundancy and restrained information transfer. Adding to this, keeping the existing databases consistent and data integrity maintained is mainly left to human intervention which in turn is very costly, both in time and money as well as error prone. Identifying a fixed conceptual dictionary in the form of a conceptual model thus seems crucial. This paper presents an effort to integrate the mutational data from the established genomic data source HGMD into a conceptual model driven database HGDB, thereby providing useful lessons to improve the already existing conceptual model of the human genome.

Design of intelligent control strategies using a magnetorheological damper for span structure

  • Hernandez, Angela;Marichal, Graciliano N.;Poncela, Alfonso V.;Padron, Isidro
    • Smart Structures and Systems
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    • v.15 no.4
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    • pp.931-947
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    • 2015
  • This paper focuses on the design of an intelligent control system. The used techniques are based on Neuro Fuzzy approaches applied to a magnetorheological damper in order to reduce the vibrations over footbridges; it has been applied to the Science Museum Footbridge of Valladolid, particularly. A model of the footbridge and of the damper has been built using different simulation tools, and a successful comparison with the real footbridge and the real damper has been carried out. This simulated model has allowed the reproduction of the behaviour of the footbridge and damper when a pedestrian walks across the footbridge. Once it is determined that the simulation results are similar to real data, the control system is introduced into the model. In this sense, different strategies based on Neuro Fuzzy systems have been studied. In fact, an ANFIS (Artificial Neuro Fuzzy Inference System) method has also been used, in addition to an alternative Neuro Fuzzy approach. Several trials have been carried out, using both techniques, obtaining satisfactory results after using these techniques.