• 생명과학회지
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• 제21권7호
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• pp.932-938
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• 2011

Ginsenoside Rg1은 인삼에서 분리한 약물학적인 활성을 가지는 물질이다. 본 연구는 Rg1이 제2형 당뇨병 모델 동물에서 혈당과 지질대사에 유익한 효과를 가지는지를 확인하기 위한 목적으로 수행되었다. 10주령의 db/db 마우스에 Rg1을 10 mg/kg 농도로 15일간 경구투여한 결과 공복혈당이 감소하였고, 포도당 내성이 개선되었다. 특히 혈중 중성지방과 유리지방산이 유의적으로 감소하였고 혈중 HDL-콜레스테롤이 증가되었다. 또한 chimeric GAL4-PPAR${\alpha}$ receptor 활성 프로모터를 활성화시켰고 PPAR${\alpha}$ gene인 CPT-1 (carnitine palmitoyltransferase-1)과 ACO (acyl-CoA oxidase)의 발현을 증가시켰는데 이것으로 Rg1의 지질대사 개선이 PPAR${\alpha}$ 활성에 의한 지방산 산화에 의한 것임을 확인할 수 있었다. 모든 결과를 종합해 볼 때, Rg1은 제2형 당뇨병과 관련된 고혈당증과 고지혈증에 유용한 효과를 가짐을 확인하였다.

• 동의생리병리학회지
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• 제17권5호
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• pp.1217-1223
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• 2003

This study was carried out to understand the effects of Bambusae Caulis in Liquamen manufactured by different production process mixed with Silk Worm Powder on blood sugar in the db/db mice. Refined Bambusae Caulis in Liquamen D(L-BCL.D and H-BCL.D) manufactured by low or high temperature production process and original Bambusae Caulis in Liquamen producted by Hanlim pham Co. (BCL) and Silk Worm Powder were used. The effects of L-BCL.D+Silk Worm Powder, H-BCL.D+Silk Worm Powdr and BCl+SWP were observed in terms of blood sugar, creatinine, BUN, GPT in db/db mice. The amount of glucose was significantly decreased (P < 0.01) in the expremental groups compared with the control. The amount of Creatinine did not show any differences among four groups. The amount of blood urea nitrogen observed significant decrease in the case of BCL group. The amount of GPT did not show any differences among four groups.

• 한국식품영양과학회지
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• 제39권5호
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• pp.692-698
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• 2010

본 연구는 중국 광동지방에서 민간에서 널리 사용되는 Kocat-D1의 항당뇨 효능을 확인하기 위하여 진행하였다. Kocat-D1의 포도당 흡수능 증가 활성을 지방구세포인 3T3-L1을 이용하여 측정해본 결과, 0.2 nM 인슐린(낮은 인슐린농도)만 투여한 대조군보다 0.2 nM 인슐린과 $100^{\circ}C$ 열수 추출물을 0.2 mg/mL의 농도로 같이 처리하였을 때 대조군보다 포도당 흡수능이 $165.0{\pm}0.7%$로 증가하였다. 8주령의 C57BL/KsJ-db/db mice를 정상대조군(normal control, db/+ mice 비처리군), 당뇨대조군(DM control, db/db mice 비처리군) 및 Kocat-D1군(db/db mice에 Kocat-D1 $100^{\circ}C$ 열수 추출물을 350 mg/kg/day로 투여한 군)으로 나누었다. 16주간의 처리 후 체중과 식이 섭취량은 Kocat-D1군이 당뇨대조군보다 감소하였다. Kocat-D1군의 혈당은 $14.7{\pm}1.4\;mmol/L$였으며 당뇨대조군($27.1{\pm}0.2\;mmol/L$)보다 유의적 수준으로 감소하였다. 인슐린 농도는 Kocat-D1 투여군이 $0.17{\pm}0.02\;ng/mL$로 당뇨대조군의 $0.05{\pm}0.02\;ng/mL$보다 증가하였다. 신장의 glomeruli 부분의 collagen 축적을 확인하기 위하여 periodic acid-shiff base(PAS)로 염색한 결과 Kocat-D1에서 PAS로 염색된 collagen 부분이 감소한 것을 확인하였다.

• 동의생리병리학회지
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• 제17권1호
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• pp.177-182
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• 2003

This study was carried out to understand the effects of Bambusae Caulis in Liquamen on blood sugar in the db/db mice. Refined Bambusae Caulis in Liquamen C. D(BCL.C. D)manufactured by high temperature production process and Bambusae Caulis in Liquamen(H-BCL) manufactured & distributed by HANLIM PHARM.COM., LTD were used. The Bambusae Caulis in Liquamen extracted from bamboo charooal manufacturing process was filtered and refined. The effects of Bambusae Caulis in Liquamen were administered orally to mice for 6weeks and its anti-diabetic effect examined. The effects of BCL.C. D and H-BCL were observed in terms of blood sugar. creatinine. BUN and GPT in db/db mice. The results were as follows : The amount of glucose was slightly decreased (P<0.05) in the B CL.C-treated groups compared with the control. The amount of glucose was significantly decreased (P<0.01) in the BCL.D and H-BCL-treated groups compared with the control. The amount of creatinine did not show any differences among four groups. The amount of blood urea nitrogen did not show any differences in the case of BCL.C-treated groups. but observed significant decrease in the case of BCL.D and H-BCL-treated groups. The amount of GPT did not show any differences in the case of BCL.D-treated groups. but observed significant increase in the case of BCL.C and H-BCL-treated groups.

• Biomolecules & Therapeutics
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• 제21권4호
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• pp.284-289
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• 2013

Antidiabetic and beta cell-protection activities of purple corn anthocyanins (PCA) were examined in pancreatic beta cell culture and db/db mice. Only PCA among several plant anthocyanins and polyphenols showed insulin secretion activity in culture of HIT-T15 cells. PCA had excellent antihyperglycemic activity (in terms of blood glucose level and OGTT) and HbA1c-decreasing activity when compared with glimepiride, a sulfonylurea in db/db mice. In addition, PCA showed efficient protection activity of pancreatic beta cell from cell death in HIT-T15 cell culture and db/db mice. The result showed that PCA had antidiabetic and beta cell-protection activities in pancreatic beta cell culture and db/db mice.

• 생약학회지
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• 제43권4호
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• pp.308-315
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• 2012

Opuntia humifusa known as the Eastern prickly pear have been used as a treatment of burns, diarrhea, asthma, rheumatism, gonorrhea, and diabetes in alternative medicine. O. humifusa is widely cultivated in the middle and southern provinces of Korea and distributed in North America. The aim of this study is to investigate anti-diabetic effect of O. humifusa stem (OHS) water or 80% MeOH extract using 3T3-L1 adipocytes and db/db mice animal models. OHS 80% MeOH extract at a dose of $250{\mu}g/ml$ significantly increased the glucose uptake and lipid accumulation compared with the control in 3T3-L1 adipocytes. Blood glucose, plasma total cholesterol and triglyceride levels were significantly reduced by oral treatment of OHS 80% MeOH extract (200 mg/kg BW) for 6 weeks in db/db mice. Also, the oral treatment of OHS 80% MeOH extract slightly changed the plasma insulin and insulin resistance levels in db/db mice, but were no significance in comparison to control. Glucose transporter(GLUT)4 expressions of adipose tissue and muscle were significantly increased more than that in the control. Therefore, these results suggest that OHS 80% MeOH extract inhibits the blood glucose level through regulation of lipid profile, insulin resistance, and GLUT4 expression in db/db mice and its diabetic effect is effective more than water extract.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.263.2-264
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• 2002

SPH-l is a herbal medicine composing oriental prescription. We have studied the antidiabetic effect and mechanism of SPH-l in insulin-resistant diabetic db/db mice. Mice were grouped and treated for 3 weeks as follows: control group was administrated with tap water orally: treated group was administrated with SPH-l orally at dose of 500 mg/kg. SPH-l lowered plasma glucose level by 67% as compared to the diabetic control (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.260.2-260.2
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• 2002

SPH-2 is a herbal medicine composing oriental prescription. We have studied the antidiabetic effect and mechanism of SPH-2 in insulin-resistant diabetic db/db mice. Mice were grouped and treated for 3 weeks as follows: control group was administrated with tap water orally: treated group was administrated with SPH-2 orally at dose of 500 mg/kg. SPH-2 lowered plasma glucose level by 43% as compared to the diabetic control. Total cholesterol. triglyceride and free fatty acid were all reduced in SPH-2 treated group. (omitted)

• Archives of Pharmacal Research
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• 제29권8호
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• pp.666-676
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• 2006

Gangliosides are widely distributed in mammalian cells and play important roles in various functions such as cell differentiation and growth control. In addition, diabetes and obesity cause abnormal development of reproductive processes in a variety of species. However, the mechanisms underlying these effects, and how they are related, are not fully understood. This study examined whether the differential expression of gangliosides is implicated in the abnormal follicular development and uterine architecture of streptozotocin (STZ)-induced and db/db diabetic mice. Based upon the mobility on high-performance thin-layer chromatography, mouse ovary consisted of at least five different ganglioside components, mainly gangliosides GM3, GM1, GD1a and GT1b, and diabetic ovary exhibited a significant reduction in ganglioside expression with apparent changes in the major gangliosides. A prominent immunofluorescence microscopy showed a dramatic loss of ganglioside GD1a expression in the primary, secondary and Graafian follicles of STZ-induced and db/db diabetic mice. A significant decrease in ganglioside GD3 expression was also observed in the ovary of db/db mice. In the uterus of STZ-induced diabetic mice, expression of gangliosides GD1a and GT1b was obviously reduced, but gangliosides GM1, GM2 and GD3 expression was increased. In contrast, the uterus of db/db mice showed a significant increase in gangliosides GM1, GD1a and GD3 expression. Taken together, a complex pattern of ganglioside expression was seen in the ovary and uterus of normoglycemic ICR and $db/^+$ mice, and the correspoding tissues in diabetic mice are characterized by appreciable changes of the major ganglioside expression. These results suggest that alterations in ganglioside expression caused by diabetes mellitus may be implicated in abnormal ovarian development and uterine structure.

• 한국식품저장유통학회지
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• 제20권1호
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• pp.1-6
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• 2013

본 실험은 Curcuma longa L.의 비만, 인슐린 저항성 효과에 대해 알아보기 위하여 C. longa 를 50% 에탄올로 추출물을 제조한 다음 실험동물을 통하여 알아보았다. 실험동물로는 정상동물인 SD계 랫트와 2형 당뇨 모델인 db/db 마우스를 사용하였고 식이로는 고지방식이(15%fat diet)를 사용하였고 C. longa는 식이에 넣어 제공하였다. SD rat 에서 체중, 총지방량은 1.5% 울금식이군이 대조군보다 유의적으로 감소하였다. 혈청에서 인슐린, HOMA-IR, TG는 1.5% 울금식이군이 대조군보다 유의적으로 감소하는 것을 관찰할 수 있었다. db/db 마우스에서 체중은 1.5% 울금식이군이 대조군보다 감소하는 경향을 보였으나 유의적이지는 않았고 총지방량은 1.5% 울금식이군이 대조군보다 유의적으로 감소하는 것을 관찰 할 수 있었다. 본 실험에서 공복 시 혈당은 2주마다 측정하였는데 1.5% 울금식이군이 대조군보다 유의적으로 감소하였고 hemoglobin A1c도 유의적으로 감소하는 것을 관찰 할 수 있었다. 공복 시 혈당과 hemoglobin의 유의적인 감소로 인하여 혈청에서 측정한 insulin, HOMA-IR이 감소하는 경향을 보인 것으로 사료된다. 이상의 결과로 보아 C. longa 추출물은 체중조절 및 혈당조절에 도움이 되는 천연약물로서의 기능성이 입증되었다고 사료된다.