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Changes in Lymphocyte DNA Damage and Antioxidant Status after Supplementing Propolis to Korean Smokers: A Placebo-Controlled, Double-Blind Cross-Over Trial (프로폴리스 섭취 후 흡연자의 임파구 DNA 손상도 및 항산화 상태의 변화: 이중맹검 교차 인체시험)

  • Kang, Myung-Hee;Lee, Hye-Jin;Kim, Mi-Kyung;Sung, Mi-Kyung;Kwon, O-Ran;Park, Yoo-Kyoung
    • Journal of Nutrition and Health
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    • v.42 no.5
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    • pp.442-452
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    • 2009
  • Smoking has been known to exacerbate the initiation and propagation of oxidative stresses. Efforts have been made to reduce the smoking-induced oxidative stresses using commercial dietary supplements. Propolis is the resinous substance collected by bees from the leaf buds and bark of trees, especially poplar and conifer trees. In this trial, we examined whether a daily supplementation of 800 mg propolis can protect endogenous lymphocytic DNA damage and modulate antioxidative enzyme activities and the level of antioxidant vitamin in smokers using a placebo-controlled, doubleblinded cross-over trial. After two weeks of running-in period, 29 smokers (mean age 34.38 ${\pm}$ 1.73) received 6 tablets/day of either propolis or placebo pills for 4 weeks. After 2 weeks of washout period the subjects switched they pills for cross-over study. The degree of DNA damage (assessed by tail DNA, tail length and tail moment) was not significantly changed with propolis intake or placebo intake. Similarly, total antioxidant status (TAS) remained at the same level regardless of the treatment. Erythrocyte catalase, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), plasma vitamin C and tocopherol level did not differ before and after propolis treatment, and did not differ between treatments. Putting all these results together, we would suggest that it is still too early to claim that propolis possess antioxidative activities.

HEARING OF RAINBOW TROUT TO COMMERCIAL SIZE IN A INDOOR AQUARIUM (실내수조를 이용한 무지개송어의 사육실험)

  • KIM In-Bae;JO Jae-Yoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.233-238
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    • 1978
  • Rainbow trout were reared in a stainless steel aquarium from Nov. 11, 1977 to June 12, 1978, and the following results were obtained : 1. The volume of water was about $400\iota$ in a aquarium measuring $1m\;(Length)\times1m\;(Width)\times67cm(Height)$ and water depth 40 cm. Water was supplied for about 16 hours daily at a rate $3\iota/min$ and was drained through the conical settling part in the middle of the aquarium bottom. Filter tank was about $23cm(W)\times23cm(L)\times40cm(D)$ and contained pebbles 30 cm in depth. Water recirculation rate was at)out $1,030\iota/hr$, or 2.6 turn-over per hour. 2. During the first period (77 days), the trout grew from 88.3g to 229g in average, the total weight attaining 30.7kg. The food coefficient was 1.249, average daily increment 243.3g, average daily growth rate 1.245%, and the mortality was 2 smallest fish weighing 53 g, owing to unknown reason. During the second period (135 days), the trout grew from 239g to 555g in average, the total weight attaining 57.2 kg. The food coefficient was 1.447, average daily increment 279.8g, average daily growth rate $0.65\%$ and the mortality was 31 fish weighing 11,255 g, owing partly to miss-handling and partly to disease. 3. The feed consisting of fully domestic materials was prepared in this laboratory, and the feed conversion was not inferior to high protein commercial feed available in foreign countries. 4. The result of whole period for 212 days was 56.5 kg in gross increment, and based on this result, when $1\iota/min$ full day inflowing water available, the net production will become 28.25 kg. So, if a 5000kg production is planned, $180\iota/min$ or about $10.8m^3/hr$ be reauired, and the production in value frill become 15million won at local price at the expense of about 5.3 million won. From the result of this experiment, rainbow trout is feasible for commercial production in Korea with relatively small amount of well water and simplified water recirculation system.

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Effects of Exercise Program by Type on Balance Ability and Muscle Activity In A Standing Posture (유형별 운동프로그램이 선 자세에서의 균형능력과 근활성도에 미치는 영향)

  • Kang, Jeong-Il;Park, Jun-Su;Park, Seung-Kyu;Yang, Dae-Jung;Choi, Hyun;Jeong, Dae-Keun;Kwon, Hye-Min;Moon, Young-Jun
    • Journal of Digital Convergence
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    • v.12 no.7
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    • pp.411-418
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    • 2014
  • This study, in order to establish the effect of three exercise groups on the static and dynamic balancing abilities and the muscular activity, targeting adult males aged 20-29, assigned 15 men to each aquatic exercise group, trunk stabilization exercise group, and balance exercise group. The study was conducted from June, 2013 to August, 2013, and measured and compared the balancing ability and the muscle activity(tibialis anterior and gastrocnemius muscle) of the participants after performing intervention for 30 minutes a day, 3 days a week, for 6 weeks. As a result, on the comparison between before and after the intervention, there were significant differences in changes of the surface area and the whole path length in all the three groups(p<.05)(p<.01), and also on the dynamic balance, there was a significant difference in change of limited of stability(p<.05)(p<.01). On change of the muscle activity of tibialis anterior, both left and right sides showed statistically significant differences in all the three groups(p<.05)(p<.01), and gastrocnemius muscle showed a statistically significant difference in all the three groups except for the left side of the trunk stabilization exercise group(p<.05)(p<.01). It could be established that aquatic exercise is effective for improvement of the balancing ability and increase of the muscular activity, and we intend to propose specific aquatic exercise program development by conducting a study to determine the objective effect of aquatic exercise on the elderly or patients who have a poor balancing ability.

Studies on the Morphological, Anatomical and Physiological Characters in Populus alba × glandulosa and its Parent (Populus alba × glandulosa 와 그의 양친종(両親種)의 형태(形態), 해부학(解剖學), 생리학적(生理学的) 연구(硏究))

  • Kim, Chung Suk;Chon, Sang Keun;Hwang, Jin Sung
    • Journal of Korean Society of Forest Science
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    • v.49 no.1
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    • pp.11-31
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    • 1980
  • The morphological, anatomical and physiological traits were eximined for Populus alba ${\times}$ glandulosa which is an important planting species in Korea. The results obtained are as follows: 1. External characters in the leaf shape and chaff shape in the catkin were inherited as incomplete dominance but nectar gland was inherited as dominance. 2. Among the 15 selected clones, 9 clones were male, 2 clones female and 2 clones monoecious. 3. There were well-developed cork layers and bast fiber bundles in the bark. 4. Primordial leaves composed of 3 layers of cells and those undifferentiated into palisade and spongy parenchymas differed in its origin. 5. Leaf scare consisted of two kinds of tissues; one is connected to vascular bundle and the other not to vascular bundle. Tissues which had been connected to vascular bundle were isolated with only 2 or 3 layers of cork cells from the outside. 6. There was complicated arrangement in the vascular bundle of petioles. 7. Growth of the hybrid was sensitively influenced by external temperature, day-length and amount of light. In particular, it was apparent in height growth. 8. Flatness, loam soils and a $60{\times}60cm$ spacing might be best factors for the growth of P. alba ${\times}$ glandulosa. 9. The rooting of 15 clones was dependant upon external factors. 10. The growth of P. alba ${\times}$ glandulosa was best at around 80% of soil moisture content on the basis of plot water capacity. 11. Temperature difference between inside and outside stems below 100cm during the winter was the greatest at the south among seasons and among directions. 12. The sap movement was markedly influenced by air temperature, relative humidity in forest stand and moisture content in stem. 13. Total sugars in the cortex changed with season but did not differ in the dircetion of the stem. 14. Isoperoxidase variations in the leaf were different among 15 clones. Thus, it may be useful as a criterium for clonal identification. 15. The rate of soil moisture content decreased at a rapid slope was faster than that at a slow slope. Poor growth of P. alba ${\times}$ glandulosa at the slope was probably due to depletion of soil moisture.

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Growth Characteristics of Cucumber Scion and Pumpkin Rootstock under Different Levels of Light Intensity and Plug Cell Size under an Artificial Lighting Condition (인공광형 폐쇄형 육묘시스템 내 광량 및 플러그 트레이 규격에 따른 오이 접수 및 호박대목의 생육특성)

  • Jang, Yoonah;Lee, Hye Jin;Choi, Chang Sun;Um, Yeongcheol;Lee, Sang Gyu
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.383-390
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    • 2014
  • This study was conducted to investigate the growth characteristics of cucumber scion and pumpkin rootstock under different levels of light intensity (photosynthetic photon flux, PPF) and plug cell size in a closed transplant production system with artificial lighting. Cucumber scion and pumpkin rootstock seedlings were grown under the combinations of three levels of PPF (PPF 165, 248, and $313{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and five types of plug tray (50, 72, 105, 128, and 200 cells in the tray) for nine days. The shoot dry weight and relative growth rate increased with increasing PPF and plug cell size. As PPF increased, cucumber scion and pumpkin rootstock seedlings had higher dry matter, lower specific leaf area, and lower hypocotyl length. The first true leaf of cucumber scion and pumpkin rootstock unfolded at eight and seven days after sowing, respectively, except the treatment using 200-cell plug tray. The unfolding of first true leaf of seedlings grown in 200-cell plug tray was delayed by one day. Accordingly, it was considered that the use of small cell size such as 200-cell plug tray would require more time for the production of scion and rootstock. Based on the results, we suggest that cucumber scion and pumpkin rootstock be grown in 105-cell to 128-cell plug tray for eight days and 72-cell to 105-cell plug tray for seven days, respectively, when using splice grafting method with root-removed rootstock. Additionally, higher PPF is suggested to improve the growth and quality of scion and rootstock.

The Effects of RPE of Step Aerobics on the Immunologic Function of High School Girls (Step aerobics의 RPE가 여고생의 면역기능에 미치는 영향)

  • Kwon, Sun-Ok;Jeong, Seon-Tae
    • Journal of Life Science
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    • v.20 no.2
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    • pp.304-313
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    • 2010
  • Out of the tenth graders of K girl's high school in J city, 24 students whose %fat was over 30% were divided into 3 groups through Purposing Sampling. Groups A and B were exercise groups and C was the control group. Using Borg's RPE (rating of perceived exertion), RPE 15-17 (hard-very hard) $\times$ 3 sets were set up for group A, RPE 11-13 (fairly light-somewhat hard) $\times$ 3 sets were set up for group B, and both groups performed step aerobics (step box: 68cm in length, 28cm in width, 15cm in hight, 450g in weight) for 50-60 minutes a day, 3 days a week for 8 weeks in total. This research was conducted to find out the effects of various RPE in step aerobics on the immunologic function (neutrophil, lymphocyte, monocyte, eosinophil, basophil, IgG, IgA, and IgM levels) of overweight female high school students. By using SPSS Ver. 14.0, a repeated two-way ANOVA was conducted to find out the effects of interaction between the groups and time period, paired t-test to evaluate data within each group, and pre- and post experiment difference rates (%diff) to perform one-way ANOVA for group comparisons. The following results were found. As for WBC, within group A, neutrophil, monocyte, basophil, and eosinophil levels increased, while lymphocyte levels remained the same. Within group B, eosinophil levels decreased while neutrophil, lymphocyte, monocyte, and basophil levels showed no differences. Within the control group, neutrophil, basophil, and eosinophil levels decreased while lymphocyte and monocyte levels showed no differences. As for the group comparisons, neutrophil levels increased more in group A than group B and the control group. There were no differences in lymphocyte levels among the three groups. Monocyte levels increased more in group A and B than the control group. Basophil and Eosinophil increased more in group A than group B and the control group. As for immunoglobin, within group A, the IgG level increased but the levels of IgA and IgM did not change. Within group B, the IgA level increased but the level of IgG decreased, and the level of IgM did not change. Within the control group, the IgG level decreased but the levels of IgA and IgM did not change. As for the group comparisons, the level of IgA increased more in group A than the control group, and the level of IgG increased more in group A than group B and the control group, but levels of IgM among the three groups did not show any difference. In summary, WBC and Ig levels showed that the three groups remained at the reference interval even after the exercise program. However, group A, which performed RPE 15-17 in step aerobics, showed increase in more measured items than the other groups, and this implies that the immunologic function has improved in the range of the reference intervals. Therefore, it will be effective to conduct step aerobics with the RPE 15-17 (hard-very hard) in order to increase the immunologic function.

Study of the Korean Americans Housewives' Knowledge of Korean Festival Foods (재미 한인 주부들의 한국 명절음식에 대한 인식)

  • 심영자;김정선
    • Korean journal of food and cookery science
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    • v.14 no.2
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    • pp.148-158
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    • 1998
  • This study was to investigate the knowledge of Korean festival foods and the acculturation of American main traditional occasions among Korean American housewives residing in the New York and New Jersey area. Out of 400 questionnaires, the 271 respondents were taken. Of the respondents, 77.1% demonstrated the interest in Korean festival foods. The most important Korean festivals were Chusuk (52.0%), Sulnal(46.9%) and Daeborum (1.1%). In order to make the Korean festival foods to be international foods, the most vital factor was taste and flavor (39.5%), cooking method (34.7%), preservation (10.3%), sanitation (8.1%) and nutrition (7.4%). American Thanksgiving Day was the most important occasion among the Korean American housewives. The factors that keep the American traditional occasions were related to whether the respondents had an occupation or the length of stay in the U.S.A. But in case of Korean festival foods, the factors cannot be applied. On the contrary, they do still keep the tradition and the interest of Korean festival foods in U.S.A. As a result of that, the Korean festival foods in the U.S.A. are systemized for the preservation and popularization for the coming Korean generation.

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Biological Study on the Increment of Survival Rate during Early Life Cycle in th Rockfish, Sebastes schlegeli (Telostei: Scorpaenidae) - I. Effects of Ammonia on Survival and Growth of the Larvae and Juveniles Stage (조피볼락, Sebastes schlegeli의 초기생활사 동안 생존율 향상을 위한 생물학적 연구 - I. 자어의 생존과 성장에 미치는 암모니아의 영향)

  • Chin, Pyung;Shin, Yun-Kyung;Kim, Hak-Gyoon;Lee, Jeong-Sick;Kim, Hyung-Soo
    • Korean Journal of Ichthyology
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    • v.10 no.1
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    • pp.98-105
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    • 1998
  • The effect of ammonia on survival and growth of the larval rockfish, Sebastes schlegeli was examined by a static renewal bioassay method. The tolerance of larvae to ammonia toxicity was more sensitive at the early larvae, but increased with the development of larvae. In 14 day-old-larvae after parturition, the mortality with treatment of each concentration of ammonia was 5% at control group and 0.0112 $NH_3$mg/l, whereas it was increased up to 27.5% at exposure group of 0.1230 $NH_3$mg/l with higher concentration. Regression equation between ammonium concentration(X) and mortality(Y) was followed; Y=0.516+3.482 X($r^2=0.4737$, P<0.01). The NOEC(no-observable-effect concentration) and LOEC (lowest-observable-effect concentration) to mortality compared to control group were 0.100 $NH_3mg/l$ and 0.1230 $NH_3$mg/l, respectively and chronic value(ChV) which is the geometric mean of the NOEC and LOEC was 0.1110 $NH_3$mg/l. Body length after 7-days exposure in control group, 0.0112 $NH_3$mg/l and 0.1230 $NH_3$mg/l were 7.8325mm, 7.700mm and 7.05mm, respectively. The NOEC, LOEC and chronic value(ChV) were 0.0335 $NH_3$mg/l, 0.0558 $NH_3$mg/l and 0.0432 $NH_3$mg/l, respectively.

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Ecological Investigations on the Managed Forests in Northwestern Europe and Tree Seed-Orchard System (북구(北歐)의 우량임분(優良林分)의 생태(生態) 및 채종원(採種園) 체계(體系)에 관(關)한 연구(硏究))

  • Kim, Chi Moon
    • Korean Journal of Agricultural Science
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    • v.9 no.1
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    • pp.239-249
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    • 1982
  • The outlines of superior stands, including species mixed, age classes, canopies, and ecological conditions of the stands, were examined in the forests of west Germany, Sweden, and Netherland. The establishment schemes of tree seed orchards for the production of high genetic quality seed were also investigated in the present study. 1. The meteorological conditions, abundant in precipitation and high in relative humidity, prove it to be oceanic climate characteristics in the Scandinavia located in high latitude and north-western part of Europe. The photo periodical characteristics following to day-length and the frost times determine the patterns of tree growth and distribution as well as the tree adaptability due to bud-setting and bud-flushing in Sweden forest. 2. The European forests are generally characterized by dense multistoried forest type composed of upper stories of tall tree species such as Norway spruce, fir, Scots pine and beech, lower stories of shade tolerant poles and saplings of spruce, fir, beech, and floor vegetations covered with birch, oak and hornbeam etc. 3. The single tree selection method and group shelterwood method are commonly applied for silvicultural system but shelterwood method and clearcutting method practiced partially in limited area. Badischer Femelschlagbetrieb and Bayerischer Femelschlagbetrieb are popularized to the regeneration of Schwarzwald but selection method applied in part. 4. Tree cuttings are properly limited so as not to exceed annual increment volume for the sustained yield and the preservation of ecosystem in European forest management. Land preservation and recreation function are more interested with the promotion of wood production in the forest management. 5. Careful attentions are paid to the reforestation of the introduced exotic species, such as Douglas-fir, red oak, pines from America, Japanese larch, Italian poplar etc., with concern against the insect and pest damage, and the meteorological damage. 6. Some intensive studies on tree improvement, such as selection of plus tree, selection of plus stand for seed collection, provenance trial, progeny test of clone, hybrid seed orchard for clone complexing, are performed in the countries and the great part of the seed required for reforestation are supplied from the seed orchard established in Sweden and Netherland.

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Effect of Slow-release Fertilizer Levels of Rice in Different Cultural Methods (벼 재배양식에 따른 유효성 비료의 시용량이 생육과 수량에 미치는 영향)

  • 정진일;최원영;최민규;이선용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.747-756
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    • 1995
  • To find out the optimum application method of slow-releasing fertilizer(SRF) and conventional fertilizer(CF) with different fertilization rate under two culture methods[l0-day old seedling machine transplanting(MT) and direct-sowing on dry paddy(DS)] in the south-western region(clay loam soil) of Korea, used were Chosun slow-releasing fertilizer(silicate latex coated fertilizer: N-P$_2$O$_{5}$-K$_2$O =18-12-13) and conventional fertilizer. Plant height and number of tillers with different two culture methods were higher at MT than DS in early growth. The ratio of dry weight in heading stage was higher at CF than SRF in MT than DS and especially, SRF 80% + CF 20% than SRF 100% or CF 100%. Leaf area index (LAI) in heading stage was higher at CF in MT but higher at SRF in DS than their counterparts. Chlorophyll content was higher at SRF than in CF expect for heading stage(HS), especially in DS. It was highest at HS in CF without its difference during maximum tillering stage(MTS) and panicle formation stage(PFS), while highest at PFS in SRF with tendency of gradual increase and decrease before and after PFS, respectively. Heading was delayed 2~3 days at SRF in two cultrue methods and 4~5 days at SRF in DS in comparison with CF in MT with delay of 2 days at DS compared with MT. Culm length was longer at CF in MT and at SRF in DS than their counterparts. Panicle number per m was more at SRF and in DS. Filled grain ratio was higher at CF and in MT. Yield was obtained 101 and 100% at 100% and 80% level of SRF in DS respectively, and 96% at 80% level of CF in MT, compared with conventional application method (516kg /l0a), and increased 2~4% at DS and 0~3% at MT in SRF. Yield was high in order of 100%(SRF) =80%(SRF) + 20%(CF) > 100%(SRF) + 20%(CF) > 80%(SRF) at MT and 80%(SRF) + 20%(CF) =100%(SRF) > 80%(SRF) =100%(SRF) + 20%(CF) at DS.

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