• Biomolecules & Therapeutics
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• 제29권5호
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• pp.506-518
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• 2021

The imprinted tumour suppressor NOEY2 is downregulated in various cancer types, including ovarian cancers. Recent data suggest that NOEY2 plays an essential role in regulating the cell cycle, angiogenesis and autophagy in tumorigenesis. However, its detailed molecular function and mechanisms in ovarian tumours remain unclear. In this report, we initially demonstrated the inhibitory effect of NOEY2 on tumour growth by utilising a xenograft tumour model. NOEY2 attenuated the cell growth approximately fourfold and significantly reduced tumour vascularity. NOEY2 inhibited the phosphorylation of the signalling components downstream of phosphatidylinositol-3'-kinase (PI3K), including phosphoinositide-dependent protein kinase 1 (PDK-1), tuberous sclerosis complex 2 (TSC-2) and p70 ribosomal protein S6 kinase (p70S6K), during ovarian tumour progression via direct binding to vascular endothelial growth factor receptor-2 (VEGFR-2). Particularly, the N-terminal domain of NOEY2 (NOEY2-N) had a potent anti-angiogenic activity and dramatically downregulated VEGF and hypoxia-inducible factor-1α (HIF-1α), key regulators of angiogenesis. Since no X-ray or nuclear magnetic resonance structures is available for NOEY2, we constructed the three-dimensional structure of this protein via molecular modelling methods, such as homology modelling and molecular dynamic simulations. Thereby, Lys15 and Arg16 appeared as key residues in the N-terminal domain. We also found that NOEY2-N acts as a potent inhibitor of tumorigenesis and angiogenesis. These findings provide convincing evidence that NOEY2-N regulates endothelial cell function and angiogenesis by interrupting the VEGFR-2/PDK-1/GSK-3β signal transduction and thus strongly suggest that NOEY2-N might serve as a novel anti-tumour and anti-angiogenic agent against many diseases, including ovarian cancer.

• 한국지리정보학회지
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• 제13권1호
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• pp.16-27
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• 2010

가로는 도시를 구성하는 선적(線的) 요소로서 도시 발전의 축이다. 이러한 가로의 본질적 기능은 통행이 목적인 교통공간이지만 이와 함께 사람과 사람의 만남, 휴식, 오락 등의 다양한 활동을 담아내는 커뮤니티의 장으로서 중요한 역할을 하고 있다. 가로는 기본적으로 보도와 차도 그리고 가로를 에워싸고 있는 가로변 건축물로 구성되는 3차원의 공간이다. 이때 가로변건축물은 가로공간을 구성하는 중요한 물리적 구성요소로 가로변건축물의 입면은 사람들이 가로공간의 이미지를 형성하는데 중요한 변수로 작용하게 된다. 이에 본 연구에서는 가로변 건축물의 입면을 구성하는 형태구성요소를 대상으로 가로경관의 이미지에 영향을 미치는 시지각적 우세요소들을 추출하고 각각의 요소들의 만족도와 선호도를 조사/분석하여 향후 도시가로 경관계획 시 기초자료로 활용할 수 있도록 제공하는데 그 목적이 있다.

• 산경연구논집
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• 제9권3호
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• pp.57-68
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• 2018

Purpose - The purpose of this study is to classify that the quality factors for comparing the Visegrard Group with the Nordic Council have historical similarities against Germany and the Soviet Union. However, this is because in the integrated European market, the competitiveness possessed by the two groups of countries is invested in the priority order to grow. Research design, data, and methodology - The study was conducted on the research design, and the reason for trying to compare the competence of the automobile industry in the assessment of industrial capability is that the Visegrard Group focuses on automotive production and the Nordic Association focuses on the commercialization of the automobile(market). In this study, searching and quantifying indirect evidence was made through standards are more complementary in Europe since each country acts like the role of the European automotive industry for example, which is different from the realistic evaluation criteria, are more important than those of the United States(first in the world) or Germany(first in Europe). Results - The results of this study are as follows: In the global EV market U.S.(export: $ 2.62 billion /share: 36.7%), Germany($ 1.29 billion /18.1%), France($ 390 million /5.4%), United Kingdom($ 380 million /5.4%), and South Korea($ 320 million/ 4.4%). South Korea's share of the EV market is 4.4%, while TSI reaches at +0.9 which measures the comparative advantage of a specific commodity in the world trade market. There is great potential for evaluated as products processing in export competition. But, commercialization, standardization, and overall market expansion did not have a positive impact on global satisfaction. Conclusions - EVs put importance on various utilities. So this suggests that Korea's exports to the EU, including the Visegrard Group, should be more focused on marketability when illuminating with a sharing industrial system under the European Union. It is necessary to specialize in manufacturing and commercialization by country(region) to prepare sharing economy and blockchain in order to create a smart-sharing city linked on artificial intelligence, as the commercialization of electric vehicles, which will have a larger growth rate than that of manufacturing in the fourth revolutionary era.

• KSBB Journal
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• 제32권3호
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• pp.224-232
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• 2017

Resveratrol has been actively investigated as an anticancer drug since it induces cell growth inhibition and apoptosis in many cancer cells. Resveratrol acts through modulation of multiple pathways and genes. In this study, we found resveratrol reduced cell growth and mammosphere formation in MDA-MB-231 triple-negative human breast cancer cells. This suppressive effect of resveratrol is accompanied by a reduction in Bmi-1 gene expression. We also observed that knock-down of Bmi-1 gene by small interfering RNA effectively sensitizes breast cancer cells to resveratrol treatment. Our data demonstrate, for the first time, that resveratrol down-regulates Bmi-1 expression in human breast cancer cells and suggest that specific molecular targeting of Bmi-1 can be combined with a chemotherapeutic strategy to improve the response of breast cancer cells to resveratrol.

• 한국축산식품학회지
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• 제35권3호
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• pp.293-298
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• 2015

Porcine placenta extract (PPE) is known to possess anti-inflammatory properties owing to its high concentration of bioactive substances. However, the need to eliminate blood-borne infectious agents while maintaining biological efficacy raises concerns about the optimal method for sterilizing PPE. Therefore, the objective of this study was to compare the effects of the standard pressurized heat (autoclaving) method of sterilization with γ-irradiation on the anti-inflammatory effects of PPE. The anti-inflammatory actions of these two preparations of PPE were evaluated by measuring their inhibitory effects on the production of NO, the expression of iNOS protein, and the expression of iNOS, COX2, TNF-α, IL-1β, and IL-6 mRNA in lipopolysaccharide-stimulated RAW 264.7 cells. Compared with autoclaved PPE, γ-irradiated PPE showed significantly greater inhibition of NO production and iNOS protein expression, and produced a greater reduction in the expression of iNOS, COX2, TNF-α, IL-1β, and IL-6 mRNA. These results provide evidence that the sterilization process is crucial in determining the biological activity of PPE, especially its anti-inflammatory activity. Collectively, our data suggest that γ-irradiated PPE acts at the transcriptional level to effectively and potently suppresses the production of NO and the expression of pro-inflammatory cytokines.

• 문화기술의 융합
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• 제6권3호
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• pp.267-273
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• 2020

비콘(Beacon)은 근거리에 있는 스마트 기기를 자동으로 인식하여 필요한 데이터를 전송할 수 있는 무선 통신장치이다. 4차 산업혁명 시대에 대표적인 사물인터넷(IoT) 설비로 근거리 정보 전달, 모바일 위치서비스, 쇼핑, 마케팅 등 다양한 분야에 활용되고 끊임없이 진화하고 있다. 본 논문에서는 관광위치 기반 추천 정보 제공 서비스를 바탕으로, 비콘 기술을 접목하여 사용자의 관심 또는, 선호도 등에 따라 맞춤형 정보를 추천하는 시스템을 제안한다. 즉, 관광객인 사용자가 원하는 정보를 알려 주는 정보 대행자의 역할을 한다. 관광객의 니즈를 충족시키기 위해서는 지능형 관광 추천 시스템 구축이 필요하다. 본 논문에서 제안한 비콘 기술을 활용한 개인 맞춤형 한식과 관광지 추천 관리 시스템은 한국을 찾는 외국인뿐 아니라 내국인 관광객들에게 양질의 서비스를 제공할 수 있을 것으로 예상된다.

• 한국주거학회논문집
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• 제25권3호
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• pp.105-114
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• 2014

Landscape zones have been designated as aesthetic old town districts across a wide range of Nakakyo-Ku and Shimokyo-Ku, city center of Kyoto, Japan. In these districts in which traditional structures and new buildings coexist, regulations of restriction on acts such as new building's heights, shapes, materials, and colors are carried out according to local governmental landscape ordinance based on Scenic Conservation Act. And yet, minimal fulfillment of the regulations according to different designer's subjective interpretation and principle of economy is rather creating abnormal shapes not harmonized with the traditional landscape. Thus, this study aims to extract combinations between form elements of middle and high rise apartment facade that affects 'harmony' and 'mismatch' in the districts by clarifying the social rules commonly implied based on intuitive judgments (sensibility evaluation) in which human experiential knowledge is involved. As research methods, the study first analyzes the form elements of the facade through a field survey, sets up a standard model through tasks of classification and segmentation and draws computer graphic images with 99 different patterns based on it. Based on these images, this study carries out sensibility evaluation and analyzes experimental data applying the rough set theory. As a result of the analysis, the combinations of form elements that affect harmony or mismatch act greatly when the colors and shapes of the pillars, positions and the patterns of the use of the first floor are combined.

• 대한화학회지
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• 제36권2호
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• pp.212-217
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• 1992

수용액내에서 gadolinium-벤조산 착물형성 반응의 열역학적 함수값들을 이온상수 0.1M $NaClO_4$, $25^{\circ}C$ 조건하에서 구하였다. gadolinium-벤조산 착물의 안정도는 여분의 엔트로피 효과에 기인하는 것으로 판단되며, 벤조산 리간드의 경우 한 자리 리간드에 비해 높은 안정도 상수값을 나타내었다. 이러한 높은 안정도는 리간드내의 벤젠고리의 공액효과로 예측되었다. 합성된 Ga$(ben)_3$의 고체 IR스펙트럼 결과, 벤조산 리간드가 두 자리 리간드로 작용하여 킬레이트를 형성하였으며, 80${\sim}$300K 온도 범위에서의 자화율 데이터는 Curie-Weiss 법칙에 잘 적용됨을 알 수 있었다.

• 한국환경과학회지
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• 제24권4호
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• pp.437-447
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• 2015

Estimation of runoff peak is needed to assess water availability, in order to support the multifaceted water uses and functions, hence to underscore the modalities for efficient water utilization. The magnitude of storm rainfall acts as a primary input for basin level runoff computation. The rainfall-runoff linkage plays a pivotal role in water resource system management and feasibility level planning for resource distribution. Considering this importance, a case study has been carried out in the Hancheon basin of Jeju Island where distinctive hydrological characteristics are investigated for continuous storm rainfall and high permeable geological features. The study aims to estimate unit hydrograph parameters, peak runoff and peak time of storm rainfalls based on Clark unit hydrograph method. For analyzing observed runoff, five storm rainfall events were selected randomly from recent years' rainfall and HEC-hydrologic modeling system (HMS) model was used for rainfall-runoff data processing. The simulation results showed that the peak runoff varies from 164 to 548 m3/sec and peak time (onset) varies from 8 to 27 hours. A comprehensive relationship between Clark unit hydrograph parameters (time of concentration and storage coefficient) has also been derived in this study. The optimized values of the two parameters were verified by the analysis of variance (ANOVA) and runoff comparison performance were analyzed by root mean square error (RMSE) and Nash-Sutcliffe efficiency (NSE) estimation. After statistical analysis of the Clark parameters significance level was found in 5% and runoff performances were found as 3.97 RMSE and 0.99 NSE, respectively. The calibration and validation results indicated strong coherence of unit hydrograph model responses to the actual situation of historical storm runoff events.

• Computers and Concrete
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• 제22권2호
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• pp.167-182
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• 2018

The modeling of loss of bond between reinforcing bars (rebars) and concrete due to corrosion is useful in studying the behavior and prediction of residual load bearing capacity of corroded reinforced concrete (RC) members. In the present work, first the possibility of using different methods to simulate the rebars-concrete bonding, which is used in three-dimensional (3D) finite element (FE) modeling of corroded RC beams, was explored. The cohesive surface interaction method was found to be most suitable for simulating the bond between rebars and concrete. Secondly, using the cohesive surface interaction approach, the 3D FE modeling of the behavior of non-corroded and corroded RC beams was carried out in an ABAQUS environment. Experimental data, reported in literature, were used to validate the models. Then using the developed models, a parametric study was conducted to examine the effects of some parameters, such as degree and location of the corrosion, on the behavior and residual capacity of the corroded beams. The results obtained from the parametric analysis using the developed model showed that corrosion in top compression rebars has very small effect on the flexural behaviors of beams with small flexural reinforcement ratio that is less than the maximum ratio specified in ACI-318-14 (singly RC beam). In addition, the reduction of steel yield strength in tension reinforcement due to corrosion is the main source of reducing the load bearing capacity of corroded RC beams. The most critical corrosion-induced damage is the complete loss of bond between rebars and the concrete as it causes sudden failure and the beam acts as un-reinforced beam.