• 생태와환경
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• 제47권4호
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• pp.292-301
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• 2014

Akinete germination may be a starting point of some akinete-producing cyanobacterial blooms in eutrophic freshwater systems. This study examined germinability of akinete of a cyanobacterium Anabaena circinalis isolated from the sediment of the North Han River (Cheongpyung Dam), Korea, under several environmental factors such as temperature ($5{\sim}25^{\circ}C$), light ($0{\sim}100{\mu}mol\;photons\;m^{-2}\;s^{-1}$), nutrients (nitrogen and phosphorus) and pH (5~12). The high germination rate appeared at high temperature: >55% at $25^{\circ}C$, followed by 15% at $15^{\circ}C$, $10^{\circ}C$ and 10% at $5^{\circ}C$. Low light intensity was favorable for akinete germination. Over 45% of germination occurred at low light intensities (5, 15 and $30{\mu}mol\;photons\;m^{-2}\;s^{-1}$), while less than 10% of germination occurred at both 50 and $100{\mu}mol\;photons\;m^{-2}\;s^{-1}$. No germination occurred in the dark condition. Akinete germination rate increased with nutrient (phosphorus and nitrogen) enrichment, and nitrogen addition showed greater effect on the germination compared to phosphorus addition. Akinetes germinated well at neutral or slightly alkaline pH condition (pH 7 and pH 8: >55%), but no germination was observed at pH 11~12. The present study demonstrates some favorable ambient conditions of Anabaena circinalis germination, which could provide useful information to study the germination conditions of other Anabaena species or akinete-forming algae and predict its bloom in eutrophic freshwaters.

• 농업과학연구
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• 제12권1호
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• pp.47-54
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• 1985

본(本) 조사연구(調査硏究)는 충남도내(忠南道內) 36개지역(個地域)에서 수집(蒐集)한 1984년도산(年度産) 메밀종자(種子)에 대(對)하여 몇가지 기초적(基礎的)인 특성(特性)을 조사분석(調査分析)하고 그들의 지역적분포(地域的分布)를 밝히고자 실시(實施)하였던 바 그 결과(結果)를 요약(要約)하연 다음과 같다. 1. 종자(種子)의 입형(粒型)은 유시형(有翅型), 중간형(中間型) 및 보통형(普通型)등 3개형(個型)이었고 어느 지성(地城)에서 수집(蒐集)한 종자(種子)이든 이들 3개형(個型)의 종자(種子)가 섞여 있었는데 그 혼합비율(混合比率)은 전체(全體) 평균(平均)으로 볼 때 보통형(普通型)이 50.1%, 중간형(中間型)이 26.8% 그러고 유시형(有翅型)은 23.1%이었으나 이러한 혼합비율(混合比率)은 지역간(地域間)에 큰 차이(差異)가 있었다. 2. 1000 입중(粒重)은 전체평균(全體平均) 24.9gr이었으나 지역간(地域間)에 큰 차이(差異)가 인정(認定)되어서 서산군(瑞山郡)((No.30)에서의 수집종자(蒐集種子)가 17.4gr로 가장 가벼웠고 서천군(舒川郡)(No.16)에서의 수집종자(蒐集種子)는 31.9gr로 가장 무거웠는데 입형별(粒型別) 혼합비율(混合比率)과 1000입중(粒重)과는 일정(一定)한 경향(傾向)이 나타나지 않았다. 3. $5^{\circ}C$로부터 $30^{\circ}C$사이에서는 온도(溫度)가 높아짐에 따라서 일반적(一般的)으로 발아(發芽)가 촉진(促進)되었는데 서천군(舒川郡)(No.16), 홍성군(洪城郡)(No.23), 천원군(天原郡)(No.35) 및 예산군(禮山郡)(No.27)에서의 수집종(蒐集種)은 $15^{\circ}C$ 그리고 서산군(瑞山郡)(No.29), 당진군(唐津郡)(No.32) 및 청양군(靑陽郡)(No.21)에서의 수집종(蒐集種)은 $25^{\circ}C$에서 발아율(發芽率)이 낮았다가 그 이상(以上)의 온도(溫度)에서 다시 상승(上昇)하였으며 $5^{\circ}C$의 저온(低溫)에서는 대부분(大部分)의 수집종자(蒐集種子)들이 10%미만(未滿)의 발아율(發芽率)을 나타내었으나 아산군(牙山郡)(No.33)과 대덕군(大德郡)(No.3)에서의 수집종(蒐集種)은 각각(各各) 20%와 30%의 발아율(發芽率)을 나타내었다. 4. 종피(種皮)의 색(色)은 흑색(黑色)과 암갈색(暗褐色)으로 구분(區分)되었으며 입형(粒型)과 종피색(種皮色)을 조합(組合)한 종자형(種子型)은 유시흑색(有翅黑色), 중간흑색(中間黑色), 보통흑색(普通黑色), 유시암갈색(有翅暗褐色), 중간암갈색(中間暗褐色) 그리고 보통암갈색종(普通暗褐色種) 등 6개형(個型)으로 분류(分類)할 수 있었고 종피색(種皮色)의 차이(差異)에 따른 광(光)Energy의 흡수(吸收) Spectrum의 차이는 190~390nm범위 이었다.

• 한국약용작물학회지
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• 제24권3호
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• pp.177-182
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• 2016

Background: Many Taraxacum spp. plants are widely used in medicine, but some of them have propagation problems, such as strong dormancy and poor germination rates. This study investigated the effects of temperature, gibberellic acid ($GA_3$), and potassium nitrate ($KNO_3$) on seed germination in Taraxacum ohwianum. Methods and Results: The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were $4.54{\pm}0.032mm$ and $0.97{\pm}0.029mm$, respectively. Various germination temperatures were tested (15, 20, 25, and $30^{\circ}C$), the optimum temperature for germination was found to be $20^{\circ}C$ (31.3%). High temperature ($30^{\circ}C$) resulted in non-typical seedlings (thickened and crumpled cotyledons, with a restricted root system). To increase the germination capacity, $GA_3$ (200, 500, and $1,000mg/{\ell}$) or $KNO_3$ (20, 50, 100, 200, and $500mg/{\ell}$) solutions were used as pre-soaking solutions instead of water. The $GA_3$ treatments increased the germination percentage and rate, but germination percentage was higher with the $KNO_3$ treatments. Under the $50-200mg/{\ell}\;KNO_3$ treatments, the germination percentage exceeded 80% after 12 days, and $50mg/{\ell}\;KNO_3$ was notably effective (91.2% after 15 days). Conclusions: T. ohwianum seeds showed improved germination at low temperatures. The best method for germination was presoaking in $50mg/{\ell}\;KNO_3$ for 24 h, in the dark at $4^{\circ}C$, and then incubating the germinated seed at $20^{\circ}C$ for 15 days.

• 한국환경생태학회지
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• 제12권1호
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• pp.9-13
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• 1998

조경용 소재로 그 관상 가치가 뛰어난 자생 갯까치수영의 종자는 저장기간이 길수록 발아력이 감소하며 그로 인한 보금에 어려움이 많았다. 본 실험에서는 우선 갯까치수영의 적정발아조건을 구명하고 프라이밍처리에 따른 발아상을 알아보기 위하여 실시하였다. 광과 온도에 따른 종자발아율을 알아본 결과 25$\circ $C의 온도조건과 광조건이 발아에 적정환경으로 이때 발아율과 발아속도는 각각 96.9%와 3.5일이었다. 20$\circ $C의 온도조건에서도 90%이상의 발아율을 보였으나 발아속도가 6.3일로 현저히 길어졌다. 저장기간이 길어졌을 때(300일) 75%까지 떨어졌던 종자의 발아율은 NaCl용액과 KNO$_{3}$ + K$_{3}$H$_{2}$PO 혼합용액을 프라이밍 처리한 결과 모든 처리농도에서 90% 이상으로 회복되었다. 여러 농도의 priming처리 후 발아속도에 차이가 있었는데, 처리농도가 각각 0.1M에서 0.5M로 높아질수록 발아속도가 길어지는 경향을 나타내었다.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.199-204
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• 2008

Seed dormancy behavior of weed seeds is a critical determinant of their survival rates in a given cropping system as it helps the weeds to evade herbicides and other weeding practices. We investigated the effects of red light, alternating temperature, stratification duration and different doses of nitrogen containing compounds alone or in combination with red light on breaking seed dormancy of Chenopodium album L. The application of red light(80 ${\mu}mols^{-1}m^{-2}$) significantly increased seed germination of C. album in all treatments. Germination rates of 12 h incubated seeds were highest under 20 min of red light irradiation than 1 min, 5 min and 10 min treated seeds. Germination rate was significantly higher at alternating temperatures of $25^{\circ}C\;and\;5^{\circ}C$ for 12 h each with an irradiation of red light(80 ${\mu}mols^{-1}m^{-2}$) for 10 min than other treatments. Stratification period of 15 days significantly stimulated germination percentage of seeds incubated in dark, although 5 days of stratification along with red light application for 10 minutes exhibit similar effects on seeds. Seed germination was also enhanced by nitrogen containing compounds like $NaNO_2,\;KNO_3,\;NH_4Cl\;and\;NH_4NO_3$. We observed that seed germination increased significantly with 25 mM $KNO_3$ and 10 mM $NH_4NO_3$ in dark condition, while $NaNO_2$ and $NH_4Cl$ enhanced seed germination under red light irradiation. It was concluded that red light alone or synergized with alternating temperatures, stratification and nitrogen compounds, especially nitrite and ammonium enhanced seed germination of C. album. Thus, the red light can play a vital role in present and future weed management strategies.

• 한국자원식물학회지
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• 제10권1호
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• pp.39-44
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• 1997

몇가지 생장조절제, 온도 및 광 조건의 차이에 의한 취나물류(참취, 개미취, 수리취 미역취 곰취)의 발아특성을 살펴본 결과는 다음과 같다. 1. 수리취는 암조건에서, 곰취, 참취, 개미취, 미역취는 명조건에서 발아율이 더 높았다. 2. 생장조절제를 처리한 종자는 명조건에서 참취를 제외한 4종의 발아율이 2-10% 향상되었다. 생장조절제 가운데 BA가 가장 효과적이었으며 무처리에 비하여 수리취에서 33%, 곰취에서 20%, 개미취에서 7% 발아율을 향상시켰다. 2. 명조건에서 발아일수는 곰취가 14일로서 가장 길었고 수리취 10일, 개미취 8일, 참취 7일, 미역취 5일 순이었다. 무처리에 비하여 생장조절제 처리구에서는 곰취, 참취, 미역취에서 각각 1일씩, 개미취와 수리취에서 각각 2일씩 단축되었다. 3. 생장조절제를 처리한 종자는 암조건에서 무처리에 비하여 7-29% 발아율이 향상되었으며 BA가 가장 효과적이었다. 4. 무처리에 비하여 암조건의 생장조절제처리구에서는 개미취 (2일), 수리취(1일), 곰취(1일)의 발아일수가 길어진 참취는 5일이 단축되었다. 수리취를 제외한 나머지 4종의 취나물류 종자의 발아일수는 BA처리에서 1-5일 단축되었다. 5. 참취는 $10^{\circ}C$, 수리취와 곰취는 $15^{\circ}C$, 개미취와 미역취는 $25^{\circ}C$에서 가장 높은 발아율을 나타냈으며 발아에 효과적인 BA의 적정 농도는 수리취가 200ppm, 곰취와 개미취가 100-150ppm, 참취가 100ppm이었고 미역취는 BA농도에 따른 발아율 차이가 인정되지 않았다.

• The Plant Pathology Journal
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• 제18권6호
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• pp.328-332
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• 2002

Wheat samples showing typical spot blotch symptoms on stems and sheaths were collected from the field after physiological maturity, and were sealed in paper bags and stored in the laboratory at room temperature to study the survival of Bipolaris sorokiniana conidia on wheat straw. The materials were observed at monthly intervals to assess the conidia viability during storage. After 4 months, the frequency of individual conidia already present on wheat straw at the time of sampling was reduced and appeared to be progressively replaced by the formation of round structures consist-ing of conidia aggregates. After 5 months, distinct, individual conidia were no longer detected, and only 'clumps of conidia' were observed. These dark black aggregates or 'clumps of conidia’measured 157-170$\mu\textrm{m}$ in diameter and were grouped into boat-shaped olivacious conidia showing thick wall and measuring 50-82$\times$20-30$\mu\textrm{m}$. The germination was unipolar and below 0.5%, suggesting the occurrence of dormancy, In contrast, individual conidium produced on wheat during the growing season were 96-130$\times$16-20$\mu\textrm{m}$, slightly curved, hyaline to light pale, and euseptate with a bipolar germination reaching 98-100%. Bipolaris sorokiniana conidia produced on PDA were 55-82$\times$20-27$\mu\textrm{m}$, tapered at both ends, dark brown to olivacious, distoseptate, showed up to 1% germination, and were predominantly unipolar. Results of the present study suggest that B. sorokiniana conidia belonged to two different physiological categories corresponding to the pathogen's infection phase and its survival, respectively. The infection phase is characterized by a high germination percentage as opposed to the survival phase harboring apparent dormancy.

• 한국환경복원기술학회지
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• 제13권4호
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• pp.30-41
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• 2010

Research was initiated to investigate germination characteristics and germination pattern of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated in the study. An alternative condition for a CB germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 85% germination, and germination percentage. Germination rates were different among the treatments at the end of study. There were significantly considerable variations in early germination characteristics and germination pattern among CB varieties. The first germination in all entries was initiated between 4 and 5 DAS (days after seeding) under ISTA conditions. Generally, the first germination percentage was 5 to 25%. However, such Penn-series varieties as Penn A-1, Penn A-4 and Penncross were much more vigorous over others, resulting in 50 to 75% in the first germination percentage. It took 4 to 10 DAS in reaching to days to the 50% germination, being 5 days in difference among the varieties. Days to the 85% germination were 5.90 to 11.75 DAS under ISTA conditions, being 5.85 days in variety difference. It means 1 to 2 days after days to 50% germination. Penn A-1 was the fastest variety, while T-1 the slowest one. Considering days to the first germination, days to 85% germination, and germination pattern etc, Penn A-1, Penn A-4, Penncross and L-93 were regarded as excellent varieties under ISTA conditions. From this study, in-depth information on germination characteristics and pattern would be usefully applied for golf course design and construction, when established with CB.

• 한국환경복원기술학회지
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• 제11권5호
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• pp.79-91
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• 2008

Research was initiated to investigate germination characteristics of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated with different experiments. An alternative environment condition requiring for a CB germination test by International Seed Testing Association (ISTA) was applied in the Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$(ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$(natural conditions). In each experiment, data such as seed germinating vigor, early germination characteristics, germination speed and germination peak time were measured. Significant differences were observed in seed germinating vigor, early germination characteristics, germination speed and germination peak time. Seed germinating vigor was variable with different environments and varieties. It was 61.50 to 98.25% under ISTA conditions and 55.00 to 98.50% under natural conditions. There were considerable variations in early germination characteristics among CB varieties according to different conditions. Early germination characteristics indicated that all varieties were 1 to 4 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 70% and 90%, was much faster with Penn A-1, Penn A-4 and Penncross under ISTA conditions. But it was even faster with L-93 and Penncross when grown under natural conditions. Differences were also observed in germination peak time with varieties and growing conditions. It was 0.57 to 2.86 days under ISTA conditions and 0.74 to 1.74 days under natural conditions. Regardless of the environment conditions, the shortest variety was L-93 and the longest one T-1. Considering germinating vigor, early germination characteristics, germination speed and germination peak time, Penn A-1, Penn A-4 and Penncross were regarded as excellent varieties under ISTA conditions in terms of early establishment characteristics, while L-93, Penn A-1 and Penncross under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for the early germinating vigor, germination speed and germination peak time. Also, a proper variety selection and comprehensive site analysis for the growing environmental conditions should be done before golf course construction.

• 생물환경조절학회지
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• 제22권2호
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• pp.188-191
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• 2013

본 연구는 도깨비바늘을 새로운 채소작물로 재배하기 위한 목적으로 종자 발아에 미치는 광, 온도 조건 및 priming 처리가 미치는 영향을 구명하기 위하여 실시하였다. 실험 결과, 발아율의 경우 명조건과 암조건 모두 $25^{\circ}C$에서 발아율이 가장 높았다. 발아세 역시 명조건, 암조건 모두 $25^{\circ}C$에서 가장 높았다. 광조건은 도깨비바늘의 발아에 영향을 미치지 않았다. 종자에 priming 처리 시 $Ca(NO_3)_2$ 0.1M(68.3%) 처리구에서 무처리구에 비해 약 16% 증가하여 가장 높은 발아율을 보였다. 그 다음으로는 $K_3PO_4$ 0.1M(63.3%) 및 $KNO_3$ 0.1M(53.3%) 순으로 나타났다. 따라서, 도깨비바늘의 발아를 위하여 명조건 $25^{\circ}C$의 온도에 $K_3PO_4$ 0.1M 또는 $Ca(NO_3)_2$ 0.1M로 priming 처리를 하는 것이 효과적인 발아조건으로 판단된다.