This study was carried out to examine seed characteristics of Artemisia capillaris and the effects of different temperature and light on its seed germination, and of sowing date on its growth and yield. A seed of A. capillaris, 0.79 mm in length diameter, 0.39 mm in width diameter and 0.054 g in thousand-kernel weight, was extremely small, vertical pinstripes in the surface of seed, and long oval of dark brown in shape, and its maturity occurred September to October. A germination rate was superior at light conditions and 20 to $25^{\circ}C$ in temperature. Although its germination became shorter as the higher temperature. Its emergence rate was the highest in sowing at March 30. But the growth and yield potential was the best when sowed at March 20. The earlier the sowing date, the higher yield.
Germination and seedling elongation of soybeans [cult. ‘Williams’] were measured at two temperatures (15 and 3$0^{\circ}C$), three seed water contents (30, 50, and 70%), and with and without polyethylene glycol-8000 (PEG) treatments. A split-plot design was used with four replications. Observations were made from two hours to eight days for each treatment combination. Seeds were soaked with 30% PEG solution with 0.2% thiram at 15 C for six days, rinsed with deionized water and dried at 25.5$^{\circ}C$ for three days. Ten treated or untreated seeds were placed on Whatman No. I (9 cm) filter paper in plastic petridishes to which sufficient deionized water was added to adjust seed moisture content to the desired level. The dishes were then sealed with parafilm and placed in a continuous programmed temperature chamber under dark condition. Seedling growth did not occur at either temperature when moisture content was 50 percent or less. Osmo-conditioning with PEG showed positive effects on seedling moisture uptake and seedling growth at 15$^{\circ}C$ but little effect at 3$0^{\circ}C$. Seedling moisture content increased rapidly early in the germination period and reached 60% at 15 C for 56 hours and at 3$0^{\circ}C$ for 28 hours. Seedling growth started when seed moisture reached a critical point of 60% at 15$^{\circ}C$, however, growth started after 20 hours of germination at 3$0^{\circ}C$. Seedlings of soybeans elongated more than four times faster at 3$0^{\circ}C$ than at 15$^{\circ}C$. Water uptake during germination was characterized by two phases in this experiment.
As a fundamental study to control Alopeuclus aequalis Sobol. var. amurensis(Kom.) Ohwi., on dominant weed in barley and dry seeded rice cultivation on paddy fields, several factors which would have closer relations to its germination and emergence, were examined. The results are as follows. The dormancy of seeds were broken at dry-heat treatment of $30^{\circ}C$. The germination rate of the seeds was high in order of 15>10>20> $5^{\circ}C$ and was very low at more than $25^{\circ}C$. The emergence of A. aequalis was influenced little for the light, but had a tendency to be good on the dark condition. The seeds dried on room temperature germinated few but them treated on $30^{\circ}C$ for 24 hours were germinated over 80%. The A. aequalis occured few in the saline soil of salt concentration of more than 0.25% and the germination rate of seeds was very low on the over 0.5% solution of NaCl. On the solution of pH 6.0~12.0, the germination was not effected for pH but was very few on pH 2.0. At the paddy-lowland which have cultivated the soybean for three years A. aequalis emerged a few.
Comparisons of understory vegetation between the burned and the unburned area, and effects of fire on seed germination of Melica onoei, which increased importance value in burned area, were investigated in the black pine forest. The number of plant species in burned and unburned area was 38 and 20, respectively. Melica onoei, indigofera kirilowii, Lespedeza bicolor Miscanthus sinensis were the most abundant species in burned area. A number of seedlings and sprouts of these species were found in burned area. Standing biomass of understory vegetation in burned and unburned area was 88.7g/㎡ and 299.8g/㎡, respectively, in the immediate postfire year. However, standing biomass in burned area increased to 537.2g/㎡ and relative standing biomass of Melica onoei was the highest with a value of 25.7% in the second year Seed germination of Melica onoei in control(C), dark (D), leaf (L), leaf extract (E) treatment was 92.5%, 86.0%, 45.5% and 39.0%, respectively. However, seed germination in L+E and D+L+E treatment was 10.5% and 4.0%, respectively. It is assumed that higher importance value of M. onoei in the postfire vegetation in this study area might be due to the removal of allelopathic inhibitors and the improvement of light condition after fire.
The effects of various factors on germination of dormant Digitaria sanguinalis seeds were studied to obtain the basic informations on establishing an effective control method in upland crops. Germination of dormant seeds was most significantly promoted by light treatment, about 88-89%, and followed by the treatment of alternating temperatures (15 or 20$^{\circ}C$ to 35$^{\circ}C$), showing the similar effects like light. Removal of seed coats also increased germination of dormant seeds by 72%, next to the treatments of light and altering temperature. Potassium nitrate ($KNO_3$) at $10^{-2}M$ and concentrated sulfuric acid ($H_2SO_4$) treatment at the duration of 8 minutes resulted in 20% and 37.8% of germination of dormant seeds, respectively, which were significantly higher than that of the untreated control. Under the conditions of altering temperature, gibberellic acid (GA) at $10^{-3}M$ to $10^{-6}M$, benzylandenin (BA) at $10^{-4}$ to $10^{-7}M$ and their combination had no effect on breaking of dormant weed seeds, and instead inhibited the promotive effects induced by the alternating temperature. However, under the constant temperature at 35$^{\circ}C$ in dark, all these treatments increased germination as much as 20% over the untreated control.
This study examined the germination characteristics by collecting fruits and pretreatment of the diaspore from April 2014 to June 2015 to investigate the diaspore characteristics, seed dispersion, and seed germination characteristics of two myrmecochorous spring ephemerals (MCSE): -Jeffersonia dubia (Jb) and Corydalis remota (Cr)-. The diaspore of Jb was about 5mm-long yellow-dark brown, oblong seeds with the attached amorphous white elaiosome. The mean weights of diaspore, seed, and elaiosome were 15.86mg, 13.46mg, and 2.40mg, respectively, and the elaiosome ratio was 15.13%. The diaspore of Cr was about 1.2mm diameter and glossy black ovoid seeds with the attached white spatula-shaped elaiosome. The mean weights of diaspore, seed, and elaisome were 2.58mg, 2.05mg, and 0.53mg, respectively, and the elaiosome ratio was 20.54%. Camponotus niponensis and Formica japonica transported the diaspore of Jb while Formica japonica and Lasius japonicus transported the diaspore of Cr. The germination percentage of Jb seeds was statistically significant and had the significance level of 1% with the pretreatment and date of sowing. However, it was independent of attachment of elaiosome. The mean germination percentages of Jb seeds was 65.0% during sowing on June 20, 17.5% during sowing on August 19, and 0% during sowing on October 20. The germination percentage of Cr was statistically significant and had the significance level of 5% and 1% with the attachment of elaisome and date of sowing, respectively. The mean germination rates were 54.17% and 35.0% in the non-treatment section and the treatment section with elaisome detached, respectively. The mean germination percentages of Cr seeds was 75.0% during sowing on June 20, 53.75% during sowing on August 19, and 5.0% during sowing on October 20. Considering the fact that the ants transported the diaspores to the ant house when the fruits of MCSE were ripened and dropped the seeds, the direct seeding right after collecting may be most suitable to the characteristics of the evolution of these plant species and may be the best method to obtain the highest germination percentages. Since the ants distribute their seeds, the MCSE produces and attaches the elaiosome to the seed to maintain the symbiotic relationship with ants. The ants then transport the seed to the ant house where the environment is controlled for suitable temperature and humidity, and then the MCSE succeeds in germination after the embryo grows sufficiently in the next spring.
Growth optima and tolerance ranges of marine algae species nay vary greatly during the developmental stage. The establishment stage is clearly of great importance for each generation, affecting the performance of the adult population. The present study was aimed to determine the effect of various concentrations of inorganic nutrients and heavy metals on germination and germlings of Ulva pertusa Kjellman (Chlorophyta). Percent germination increased rapidly as irradiance level increased, reaching the maximum at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in both east and west seawater. Percent germination in the east seawater was higher than that in the west seawater at irradiances lower than 30 ${\mu}$mol m$\^$-2/s$\^$-1/, and there was no difference in percent germination between the two different seawaters at irradiances higher than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. Germling growth increased in the both cases with increasing irradiances but no growth was found in the dark. Overall germling area was larger in the east seawater than in the west seawater. Number of cell increased with increasing irradiance and became light-saturated at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in the both cases. Germlings grown in the east seawater had more cells than those cultivated in the west seawater at irradiances lower than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. In various combinations of nitrate and phosphate, percent germination increased with increasing nitrate concentrations irrespective of phosphate concentrations. At 3 days after inoculation, nitrate concentration of 2.5 ppm with all phosphate concentrations promoted germination. Area and number of cell of germlings increased with increasing nitrate concentrations in all phosphate concentrations. In various combinations of copper and lead, germination was delayed as copper concentrations increased in all lead concentrations. Percent germination was severely reduced at 1 ppm Cu$\^$2+/. Area and number of cell of germlings decreased with increasing copper concentrations in all lead concentrations. The highest copper concentration (1 ppm) clearly suppressed the germling growth in U. pertusa.
This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was $0.47{\pm}0.07mm$, width $0.16{\pm}0.006mm$, and thickness $0.12{\pm}0.01mm$. The weight of one seed was $0.0003{\pm}0.0001mg$, and 1000 seeds weighed $4.59{\pm}0.02mg$. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at $4^{\circ}C$. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at $4^{\circ}C$ were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination. The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.
Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m-2·s-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.
Gibberellin (GA) has been widely used to break seed dormancy for better stand establishment. The experiment was done to clarify the effect of $GA_3$ (concentration; period) and light quality (red; white; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum; Codonopsis lanceolata; C. pilosula) to get an information on their field emergence. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. lanceolata, C. pilosula. Their mean germination rates and radicle lengths were increased with increased concentration to 0.1mM of $GA_3$. Earlier germination rate was higher but later one was less in 4-day $GA_3$ treatment than in 1- or 2-day $GA_3$ treatment. Light treatment. especially red light given after $GA_3$ treatment. eliminated the $GA_3$ treatment effect. Red light done after $GA_3$ treatment nearly blocked the germination of P. grandiflorum and C. pilosula but delayed that of C. pilosula compared to the other light quality treatments having the similar rate. In addition. the radicle elongation of all three species affected by light quality treatment showed the same result as the germination rate.
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