• Toxicological Research
• /
• v.22 no.4
• /
• pp.423-429
• /
• 2006

Recent reports on the photocarcinogenicity and photogerotoxicity of many compounds led to an increasing awareness for the need of a standard approach to test for photogenotoxicity. The comet assay has been recently validated as a sensitive and specific test system for the quantification of DNA damage. Thus, the objectives of this study are to investigate the utility of photo-comet assay for detecting photo-mutagens, and to evaluate its ability to predict rodent photo-carcinogenicity. Photo-comet assays were performed using L5178Y $Tk^{+/-}$ mouse lymphoma cells on five test substances (8-methoxypsoralen, chlorpromazine, lomefloxacin, anthracene and retinoic acid) that demonstrated positive results in photocarcinogenicity tests. For the best discrimination between the test substance-mediated DNA damage and the undesirable DNA damage caused by direct UV absorption, a UV dose-response of the cells in the absence of the test substances was firstly fnalized. Out of 5 test substances, positive comet results were obtained for chlorpromazine, lomefloxacin, anthracene and retinoic acid while 8-methoxypsoralen found negative. An investigation into the predictive value of this photo-comet assay for determining the photocarcinogenicity showed that photo-comet assay has relatively high sensitivity. Therefore, the photo-comet assay with mammalian cells seems to be a good and sensitive predictor of the photocarcinogenic potential of new substances.

• Molecules and Cells
• /
• v.39 no.3
• /
• pp.204-210
• /
• 2016

DNA damage responses are important for the maintenance of genome stability and the survival of organisms. Such responses are activated in the presence of DNA damage and lead to cell cycle arrest, apoptosis, and DNA repair. In Caenorhabditis elegans, double-strand breaks induced by DNA damaging agents have been detected indirectly by antibodies against DSB recognizing proteins. In this study we used a comet assay to detect DNA strand breaks and to measure the elimination of DNA strand breaks in mitotic germline nuclei of C. elegans. We found that C. elegans brc-1 mutants were more sensitive to ionizing radiation and camptothecin than the N2 wild-type strain and repaired DNA strand breaks less efficiently than N2. This study is the first demonstration of direct measurement of DNA strand breaks in mitotic germline nuclei of C. elegans. This newly developed assay can be applied to detect DNA strand breaks in different C. elegans mutants that are sensitive to DNA damaging agents.

• Structural Engineering and Mechanics
• /
• v.30 no.4
• /
• pp.445-466
• /
• 2008

This paper presents a novel structural damage detection method with a new damage index based on the statistical moments of dynamic responses of a structure under a random excitation. After a brief introduction to statistical moment theory, the principle of the new method is put forward in terms of a single-degree-of-freedom (SDOF) system. The sensitivity of statistical moment to structural damage is discussed for various types of structural responses and different orders of statistical moment. The formulae for statistical moment-based damage detection are derived. The effect of measurement noise on damage detection is ascertained. The new damage index and the proposed statistical moment-based damage detection method are then extended to multi-degree-of-freedom (MDOF) systems with resort to the leastsquares method. As numerical studies, the proposed method is applied to both single and multi-story shear buildings. Numerical results show that the fourth-order statistical moment of story drifts is a more sensitive indicator to structural stiffness reduction than the natural frequencies, the second order moment of story drift, and the fourth-order moments of velocity and acceleration responses of the shear building. The fourth-order statistical moment of story drifts can be used to accurately identify both location and severity of structural stiffness reduction of the shear building. Furthermore, a significant advantage of the proposed damage detection method lies in that it is insensitive to measurement noise.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2002.11b
• /
• pp.755-761
• /
• 2002

Recently, the noise damage cases of livestock are increasing rapidly. The cases related to the livestock damage were occupied 20% of noise and vibration dispute intervention cases from 1991 to 2001. Of this results, 34% (16/38) are relative to pig, 27%(13/30) to domestic fowl and 20%(9/38) to cattle. The results of regression analysis of between the approval of damage rates (ADR) and a noise level by livestock have been derived that pigs was highly sensitive. In case of cattle, the regression model to apply the ADR according to the appraised noise level was made, but it needs to be complemented later.

• Structural Engineering and Mechanics
• /
• v.51 no.6
• /
• pp.1037-1046
• /
• 2014

Most existing damage detection methods based on curvature should investigate the indicator value changes between the intact and damaged state. However, the 'footprint' in the intact state is usually not available for most actual beams. Therefore, a new damage localization indicator called Difference of Nearby Difference Curvature Indicator (DNDCI) was introduced for single-span beams. This indicator does not require prior information of the intact beam and is sensitive to the damage occurs on or nearby the measuring points. Practical and detailed application method of DNDCI has been proposed. Besides the idealized simply supported beams, it was validated by example that DNDCI is also applicable to the actual single-span beams supported by bearings.

• Steel and Composite Structures
• /
• v.15 no.5
• /
• pp.567-583
• /
• 2013

Experimental studies and finite element analyses have been carried out to establish the effect of tendon damage on the structural behavior of concrete filled tubular tied arch girder (CFTA girder). The damage of tendon is considered in different stages by varying the number of damaged cables in the tendon. Static and dynamic structural parameters are observed at each stage. The results obtained from the experiments and numerical studies have been compared to validate the studies. The tendons whose damage can significantly affect the stiffness of the CFTA girder are identified by performing the sensitivity analysis. The locations in the girder which are sensitive to the tendon damage are also identified.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2002.11a
• /
• pp.375.1-375
• /
• 2002

Recently, the noise damage cases of livestock are increasing rapidly. The cases related to the livestock damage were occupied 20% of noise and vibration dispute intervention cases from 1991 to 2001. Of this results, 34%(16/38) are relative to Pig, 27%(13/38) to domestic fowl and 20%(9/38) to cattle. The results of regression analysis of between the approval of damage rates (ADR) and a noise level by livestock have been derived that pigs was highly sensitive. (omitted)

• Structural Monitoring and Maintenance
• /
• v.1 no.3
• /
• pp.339-356
• /
• 2014

This study has been motivated to evaluate the practicality of numerical simulation of impedance monitoring for damage detection in steel column connection. In order to achieve the objective, the following approaches are implemented. Firstly, the theory of electro-mechanical (E/M) impedance responses and impedance-based damage monitoring method are outlined. Secondly, the feasibility of numerical simulation of impedance monitoring is verified for several pre-published experimental examples on steel beams, cracked aluminum beams, and aluminum round plates. Undamaged and damaged steel and aluminum beams are simulated to compare to experimental impedance responses. An aluminum round plate with PZT patch in center is simulated to investigate sensitive range of impedance responses. Finally, numerical simulation of the impedance-based damage monitoring is performed for a steel column connection in which connection bolts are damaged. From the numerical simulation test, the applicability of the impedance-based monitoring to the target steel column connection can be evaluated.

• Structural Engineering and Mechanics
• /
• v.67 no.3
• /
• pp.245-254
• /
• 2018

In this paper, a Cloud Model based Fruit Fly Optimization Algorithm (CMFOA) is presented for structural damage identification, which is a global optimization algorithm inspired by the foraging behavior of fruit fly swarm. It is assumed that damage only leads to the decrease in elementary stiffness. The differences on time-domain structural acceleration data are used to construct the objective function, which transforms the damaged identification problem of a structure into an optimization problem. The effectiveness, efficiency and accuracy of the CMFOA are demonstrated by two different numerical simulation structures, including a simply supported beam and a cantilevered plate. Numerical results show that the CMFOA has a better capacity for structural damage identification than the basic Fruit Fly Optimization Algorithm (FOA) and the CMFOA is not sensitive to measurement noise.

• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 1997.12a
• /
• pp.101-101
• /
• 1997

Comet assay, single cell gel electrophoresis has been known as useful, rapid, simple, visual, and sensitive technique for measuring the DNA breakage in mammalian ce1ls. For evaluation of DNA damage using comet assay, early studies reported a change in comet length and intensity with DNA damage using simple visual technique, such as fluorescence microscopy with eyespiece. In recent, some workers are observing and analyzing nucleotide of comets using quantitative fluorescence image analysis system to estimate 'tail moment', which is defined as the product of the tail length and the fraction of total DNA in tail. Our laboratory also adopted the image analysis software for qualification. In addition, many of the practical features of comet assay render it potentially attractive as useful tool for molecular toxicology and carcinogenesis, because the system is already showing considerable promise as rapid predictor in both in vitro and in vivo experimental designs. Recently, the comet assay becomes a attractive technique to study of apoptosis, because apoptotic fragmentation of nuclear DNA into nucleosomal sizes can be evaluated by the comet assay. So, we attempted to apply the comet assay to studying the effect of various stress on the apoptosis-sensitive cell lines. Particularly, focusing on the hyperthermic apoptosis, we could find that heat shock(44˚C for 60 minutes) was sufficient to induced apoptosis in these cell lines. But using the highly sensitive comet assay, we could not detect DNA breaks immediately after heat shock.