The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.
This study investigates relation of food safety incidents with climate. Therefore food safety incidents and climate data during 1999 to 2009 have been analyzed. In situ observations of monthly mean temperature, maximum temperature, minimum temperature, precipitation, and relative humidity in 60 observation stations of Korean Meteorological Administration (KMA) have been used in this study. Food safety incidents data have been constructed by searching media reports following Park's method (2009) during the same period. According to the Park's method, 729 events were collected. To analyze its relations, food safety incidents data have been classified into chemical, biological, and physical hazards. Pearson product-moment correlation coefficients have been applied to analyze the relations. The correlation of food safety incidents has negative one with precipitation (-0.48), and positive one with minimum temperature(0.45). Precipitation has been correlated with biological and physical hazards more than chemical hazard. Temperatures (mean temperature, maximum temperature, and minimum temperature) have been correlated closely with chemical hazard than others. Food safety incidents data has been interblended with human behavior factor through decision-making processes in food manufacturing, processing, and consumption phases of "farm-totable" food processing. Act in the preventing damage will be obvious if the hazard were apparent. Therefore abnormal condition could be more dangerous than that of apparent extreme events because apparent events or extreme events become one of alarm over hazards. Therefore, human behavior should be considered as one of the important factors for analysis of food safety incidents. The result of this study can be used as a better case study for food safety researches related to climate change.
Shin, Hyuk Soo;Kim, Minwoo;Song, Jerry;Lee, Junseok;Ha, Yoonjeong;Jeon, Young Hee;Kim, Ji Woong;Lee, Yun Ju;Park, Soo Nam
Journal of the Society of Cosmetic Scientists of Korea
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v.44
no.4
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pp.407-417
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2018
In this study, the antioxidant, cytoprotective and antimicrobial activities of 50% ethanol extract of Polygoni multiflori Radix (PMR) and its ethyl acetate fraction were evaluated to confirm the applicability as a functional ingredient. The activities of the major constituent of PMR were verified and 2, 3, 5, 4′-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside (THSG) was confirmed to be the main component of extract and fraction using HPLC-DAD, LC-EIS-MS analysis. The phenolic and THSG contents of the ethyl acetate fraction were 11.1- and 3.0-folds higher than those of the ethanol extract, respectively. As a result of the DPPH assay and that of luminol dependent chemiluminescence assay in $Fe^{3+}$-EDTA/H2O2 system. the ethylacetate fraction was superior to the ethanol extract in free radical and ROS scavenging activities. Especially, the ethyl acetate fraction and THSG exhibited the similar scavenging activity like L-ascorbic acid in ROS scavenging activity. The ethyl acetate fraction perceived the most potent cytoprotective effect against oxidative damage of erythrocytes induced by photosensitization reaction, followed by the ethanol fraction, THSG and that of (+)-${\alpha}$-tocopherol, which was used as a positive control. Antimicrobial activities were evaluated by disc diffusion and broth microdilution assay against S. aureus, E. coli, P. aeruginosa and C. albicans. In particular, the antibacterial activity of the extract and fraction against S. aureus was superior to that of methyl paraben. Taken together, our results suggest that PMR could be used as a natural ingredient for antioxidant, cytoprotective and antimicrobial activities.
Kim, Won-Jin;Lee, Jeong-Yong;Shin, Yun-Kyung;Won, Kyoung-Mi
Korean Journal of Ichthyology
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v.30
no.4
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pp.194-204
/
2018
In order to minimize the damage on the red sea bream Pagrus major by a harmful dinoflagellate Cochlodinium polykrikoides, we investigated the effect of feeding, starvation and stocking density on the survival rate, growth, growth restoration and physiological response of P. major exposure to C. polykrikoides. The experimental groups were divided into three groups such as F-HD (feeding and high density with $6.4kg/m^3$), S-HD (starvation and high density with $6.4kg/m^3$) and S-LD (starvation and low density with $3.2kg/m^3$) according to stocking density and starvation in marine cage ($11m{\times}11m{\times}5m$). The F-HD was fed throughout the experiment for 9 weeks, whereas S-HD and S-LD were not fed for 5 weeks and then refeeding for 4 weeks. Survival rate was the lowest in F-HD (85.5%) and S-LD was the highest (97.3%). The growth rates of S-HD and S-LD were significantly lower than F-HD during starvation period for 4 weeks, but rapidly recovered after feeding. The nutritional status such as ALB, TP, TCH, TG were similar to tendency of growth data. Ht, Hb, AST, ALT and GLU levels were significantly higher in the F-HD than in the starvation groups at the same time (in 3 week) during starvation period. But starvation groups did not differ during starvation period. As a result, F-HD is more sensitive to stress than S-HD and S-LD. Thus, during C. polykrikoides bloom period, starvation and stocking density control can help survival and growth restoration of the red sea bream.
Jeong, Dae Hui;Kim, Ki Yoon;Park, Sung Hyuk;Jung, Chung Ryul;Jeon, Kwon Seok;Park, Hong Woo
Korean Journal of Plant Resources
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v.34
no.4
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pp.287-296
/
2021
Recently, the pace of global climate change has tremendously increased, causing extreme damage to crop production. Here, we aimed to examine the growth characteristics and useful components of Angelica gigas under extreme heat stress, providing fundamental data for its efficient cultivation. Plants were exposed to various experimental temperatures (28℃, 34℃, and 40℃), and their growth characteristics and content of useful components were analyzed. At the experimental site, the ambient and soil temperature were 19.38℃ and 21.34℃, ambient and soil humidity were 81.3 % and 0.18 m3/m3, solar radiation was 162.05 W/m2. Moreover, the soil was sandy-clay-loam (pH 6.65), with 2.66% organic matter, 868.52 mg/kg soil available phosphate, and 0.14% nitrogen. Values of most growth characteristics, including the survival rate (85%), plant height (38.66cm), and fresh and dry weight (41.3 g and 14.24 g), were the highest at 28℃. Although the highest content of useful components was observed at 34℃ (3.24%), there were no significant differences across temperatures. Growth characteristics varied across temperatures due to detrimental effects of heat stress, such as accelerated tissue aging, reduced photosynthesis, and delay of growth. Similar content of useful components across temperatures may be due to poor accumulation of anabolic products caused by impaired growth at extremely high temperatures.
The severe lettuce damage caused by Paratylenchus projectus was first reported in 2019 in Korea. To find high-value rotation crops for the control of P. projectus, nine vegetables, Brassica juncea (leaf mustard), B. rapa subsp. nipposinica (kyona), B. oleracea var. italica (broccoli), B. rapa subsp. chinensis (bok choy), B. oleracea var. viridis (kale), B. oleracea var. gongylodes (kohlrabi), Cichorium endivia (endive), C. intybus (chicory), Ipomoea aquatica (morning glory) were planted in d-10-cm clay pots in greenhouse. The growth of vegetables was compared between inoculated with 3,000 P. projectus per 100 ㎤ of soil and non-inoculated. Treatments were replicated 10 times. After 100 days, the reduction of fresh top weight was 30.4% in C. intybus, 35.1% in I. aquatica, 36.9% in B. oleracea var. acephala, 40.5% in C. endivia, 42.1% in B. rapa, 47.5% in B. rapa subsp. nipposinica, 50.4% in B. oleracea var. gonglodes, 56.3% in B. oleracea var. italica, and 66.0% in B. juncea. Nematode multiplication rates (Pf/Pi) were lower in I. aquatica (0.64) and C. endivia (1.1), but higher in B. oleracea var. gongylodes (2.54). Considering these results, I. aquatica is suitable for the rotation crop with lettuce until better rotation crops developed.
The purpose of the study is to examine righteous army activity and combat in Hapcheon during the Imjin invasion period. The resulting conclusions are as follows. First, Chung In-hong raised a righteous army in Hapcheon and Kim Myeon raised an army in Goryeong. Chung In-hong commanded the army and fought against Japanese army with a leadership of knowing the enemy and himself, and Kim Myeon commanded the army and fought against Japanese military with a leadership of harmony. Second, battles of Chung In-hong's righteous army are the first Mugye battle, the ambush battle in Sawon-dong, the Chogye Majin battle, the Aneon battle, and the third Seongju Castle battle. Battles of Kim Myeon's righteous army are the Yeongang battle, the Gaesanpo battle, the second Mugye battle, the Ucheok-hyeon battle, the Jirye battle, and the Sarangam battle. Battles that Chung In-hong and Kim Myeon united and fought are the first and second Seongju Castle battles. In Jeongyu Jaeran, Chung In-hong played a role of Jodosa who takes charge of provisions in the right area of Gyeongsang. In addition, Hapcheon was the access road and transport route where Japanese army entered Jeolla-do. Third, participation and role of Ming troops are part of restraint device against Japanese army with military tactics of ii chei(using foreigners to control foreigners) to remove Japanese army from Joseon and defend Yodong. After a victory of Li Rusong in Pyeongyang Castle in January, 1593(the 26th year of Seongjo), Ming troops pursued practical interest through peace talks rather than active battles. When there was practically Siege of Jinju, Yujeong troops of Ming entered with hand-to-hand martial arts of Sacheon soldiers in late June, 1593, but did not participate because they should wait for orders of Gyeongnyak Song Eung-chang and Admiral Li Rusong. Fourth, in the Imjin invasion period, Joseon suffered terrible damage such as ruin of the whole country by invasion of Japanese army and in need of aid of military rice from Ming troops, but righteous armies and the royal forces in each area cooperated so defeated Japanese army. It is understood through a case of Hapcheon in the right area of Gyeongsang. Especially, Joseon did not succumb to pressure of Ming troops that used full powers to two aspects such as settlement and battle with Japan during the war, and did not lose national confidence and pride by showing a fighting will to fight against Japanese army to the end with independence. Such a spiritual culture originated from homeland protection and loyalty to the king, and is national spirit of resistance that sublimates the united mind and spirit of community to protect a country against foreign invasion.
Won, Ok Jae;Hong, Seo yeon;Suh, Eun Ji;Park, Jae-Sung;Lee, Hong Seok;Park, Jin-Ki;Ryu, Jong-Soo;Han, Won-Young;Han, Kil Su;Song, Duk Young
KOREAN JOURNAL OF CROP SCIENCE
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v.66
no.4
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pp.358-364
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2021
This study was conducted to select a desiccant and determine its concentration for safe usage to improve the harvesting efficiency of soybeans. Soybeans were treated with a desiccant (non-selective herbicide) before and after the maturation stage. The drying effect of the desiccant was higher at earlier treatment times than at the maturation stage, but the difference was not statistically significant. The higher efficacy might be related to the drying process of the leaves and stems, with most of the leaves and stems having already been dried by the time of hand harvesting. Desiccant treatments had no adverse effects on soybean yield, weight of 100 grains, seed quality, or seed germination rate compared with the untreated control. Pesticide residue analysis showed minimum residue concentration to be lower than the tolerance level of pesticide residues. In conclusion, it was confirmed that the desiccant was effective in drying soybean, and that there was no damage to the quality of soybean seeds. In addition to the drying effect, the dessicant treatment also facilitates the removal of weeds that interfere with the mechanical harvest and improves harvesting efficiency through the drying of the growth imbalanced individual. The desiccant treatment is expected to shorten the mechanical harvesting time by 1-2 weeks. It is thought that the selection of the proper cultivation period for other crops after soybean cultivation will be more advantageous.
This study was designed to identify the actual state of naturalized plants and invasive alien species that cause disturbances to the ecosystem, plants which are introduced after forest restoration, and explore the implications resulting from the project. Onsite examination included 29 sites which have been subjected to forest restoration by the Korea Forest Service. Once these were chosen, activity took place twice a year in the spring (May-June) and in the summer (August-September) in 2020 and 2021. Areas not relevant to the project sites were excluded from this activity so that we could identify the plants that could be understood to have been introduced or brought into the site after the actual forest restoration. And the correlation was analyzed, between the naturalized flora within the project sites and the working types applied to the site through confirmation of completion of the restoration project. The naturalized plants appearing on the entire site cover a total of 109 taxa, which includes 29 families, 80 genera, 108 species and 1 subspecies, while invasive plants included 3 families, 7 genera and 8 species. The number of classifications and the naturalization rate gradually decreased over time, after the project. While there was no significant difference between the number of classification groups and the naturalization rate for naturalized plants between project sites, given the number of taxa of naturalized plants, organized by type of damage, there were relatively more naturalized plants that appeared in the severed section of the Baekdudaegan Mountain Range, as well as at quarry and facility sites. Seeding apparently results in naturalization rates as high as 15.545%, on average, based on comparisons of naturalization rates by sowing, seeding, planting, herb planting, and sod pitching channels, all of these being methods of vegetation for planting/greening of bareland and slopes within the project areas. With no seeding, it was 9.167%, higher than the average. As for other vegetation, there was no significant difference depending on application of the working type. This means that unlike the plants subjected to planting, the working type of seed planting which makes it difficult to identify whether a certain plant is a naturalized plant greatly affects the introduction of naturalized plants to the restoration sites, even when using herb planting and sod pitching to control plants and results. Therefore the study suggests that there be inspection by experts of seeds when sowing within restoration sites. The results of this study suggest good practices that will help to direct effective vegetation restoration and follow-up management.
Purpose: This study compared the change in foraminal space on magnetic resonance imaging (MRI) and the clinical outcome after anterior cervical discectomy and fusion (ACDF) versus foraminotomy in cervical foraminal stenosis. Materials and Methods: A retrospective case-control study was conducted from January 2018 to March 2019 on 186 patients who underwent ACDF and foraminotomy. One hundred and two cases were selected considering age, sex, and body mass index. MRI was performed before and on the 5th day after surgery to compare the changes in the foraminal diameter between the ACDF group (group A-51) and foraminotomy group (group B-51). Results: Between groups A and B, the average change in foraminal vertical diameter was 1.7 mm and 1.2 mm, respectively; group A was 0.5 mm larger difference (p=0.042). The average change in foraminal transverse diameter was 1.2 mm and 1.8mm, respectively; group B showed a 0.6 mm larger change (p=0.21). Both the neck disability index (NDI) and Japanese orthopaedic association (JOA) scores improved in both groups. Group A showed more improvement, but there was no significant difference (p=0.356, p=0.607, respectively). Conclusion: Foraminotomy is a useful option for patients with foraminal stenosis of the cervical spine because it showed comparable clinical and morphological results to ACDF and could minimize motion segment loss and muscle and ligament damage.
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