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http://dx.doi.org/10.5352/JLS.2019.29.6.724

Cytotoxicity of Particulate Matter in Various Human Cells Lines  

Lee, Ji-Hyeon (Science Education Center for the Gifted Gyeongsang National University)
Lee, Joo-Yeong (Science Education Center for the Gifted Gyeongsang National University)
Kim, Mi-Jeong (Department of Biology Education, Gyeongsang National University)
Kim, Hyeon-Ji (Department of Biology Education, Gyeongsang National University)
Lee, Sung-Ho (Division of Life Science, Gyeongsang National University)
Jeon, Byeong-Gyun (Science Education Center for the Gifted Gyeongsang National University)
Publication Information
Journal of Life Science / v.29, no.6, 2019 , pp. 724-734 More about this Journal
Abstract
The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.
Keywords
Air pollution; cytotoxicity; human; particulate matter;
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