• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.102-111
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• 2020

Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

• Korean Journal of Veterinary Service
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• v.38 no.4
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• pp.253-257
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• 2015

Bull-boxing in South Korea is a form of competitions in which bulls measure their strength with each other in the form of pushing by putting their heads together. Bull-boxing has been admitted after being designated as a play culture by the government because it does not cause great injuries to animals' bodies and has strong positive aspects such as contributing to livestock farmers' leisure activities, encouragement of animal husbandry, and communities' economic development. However, bull-boxing sometimes causes damage to the head and horns due to the heavy bodies. According to the results of examinations of these damage cases, damage that caused irregular shapes of the base of horns was identified in 31.5% of fighting bulls. The damage to horns is thought to have been caused by the great forces of fighting bulls repeatedly imposed to each other's horns during bull-boxing that caused minute fractures leading to damage to the blood vessels inside the horns resulting in the blocking of delivery of nutrients to the horns causing the deformation of the horns into abnormal shapes. Since bulls' bodies are injured during bull-boxing although the injuries are small as bull-boxing is mainly conducted by pushing, animal protection groups regard bull-boxing as cruelty to animals and request to stop bull-boxing. The present study aims to develop animal welfare type headgears that can protect bulls' horns and heads in order to protect fighting bulls during bull-boxing in terms of animal protection and welfare.

• Journal of The Korean Society of Agricultural Engineers
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• v.56 no.1
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• pp.41-49
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• 2014

Pig chronic wasting disease, including porcine reproductive and respiratory syndrome (PRRS) and postweaning multisystemic wasting syndrome (PMWS), have made a continuous economic damage in pig farms. Airborne spread of livestock viruses are an important spread factor which is difficult to analyze due to invisible airflow and limitation of measurement. The objective of this study is to analyze airborne disease spread between buildings in the experimental pig farm by means of field experiment and computational fluid dynamics (CFD). The field experiments were conducted to capture airborne virus using air sampler and teflon filter along multi points in the experimental pig farm. The samples were tested in terms of virus detection resulting in positive reaction for PRRS and PCV-2 viruses, which can be a firm evidence of airborne virus spread. The CFD simulation model was developed by considering complex topography, wind conditions, building arrangement, and ventilation systems and was used to analyze airborne virus spread according to different wind conditions. The CFD computed result showed a possibility of airborne virus spread via livestock aerosol from infected pig house to neighboring pig houses according to wind directions. The CFD simulation technique is expected to provide significant data for estimating and making a counterplan against airborne disease spread.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.4
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• pp.1481-1498
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• 2014

Automated activity monitoring has become important in many applications. In particular, automated monitoring is an important issue in large-scale management of group-housed livestock because it can save a significant part of farm workers' time or minimize the damage caused by livestock problems. In this paper, we propose an automated solution for measuring the daily-life activities of pigs by using video data in order to manage the group-housed pigs. Especially, we focus on the circadian rhythm of group-housed pigs under windowless and 24-hour light-on conditions. Also, we derive a cost-effective solution within the acceptable range of quality for the activity monitoring application. From the experimental results with the video monitoring data obtained from two pig farms, we believe our method based on circadian rhythm can be applied for detecting management problems of group-housed pigs in a cost-effective way.

• Toxicological Research
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• v.29 no.1
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• pp.43-52
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• 2013

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium that are found in cereals and agricultural products. ZEN has been implicated in mycotoxicosis in farm animals and in humans. The toxic effects of ZEN are well known, but the ability of an alkaline Comet assay to assess ZEN-induced oxidative DNA damage in Chang liver cells has not been established. The first aim of this study was to evaluate the Comet assay for the determination of cytotoxicity and extent of DNA damage induced by ZEN toxin, and the second aim was to investigate the ability of N-acetylcysteine amide (NACA) to protect cells from ZEN-induced toxicity. In the Comet assay, DNA damage was assessed by quantifying the tail extent moment (TEM; arbitrary unit) and tail length (TL; arbitrary unit), which are used as indicators of DNA strand breaks in SCGE. The cytotoxic effects of ZEN in Chang liver cells were mediated by inhibition of cell proliferation and induction of oxidative DNA damage. Increasing the concentration of ZEN increased the extent of DNA damage. The extent of DNA migration, and percentage of cells with tails were significantly increased in a concentration-dependent manner following treatment with ZEN toxin (p < 0.05). Treatment with a low concentration of ZEN toxin (25 ${\mu}M$) induced a relatively low level of DNA damage, compared to treatment of cells with a high concentration of ZEN toxin (250 ${\mu}M$). Oxidative DNA damage appeared to be a key determinant of ZEN-induced toxicity in Chang liver cells. Significant reductions in cytolethality and oxidative DNA damage were observed when cells were pretreated with NACA prior to exposure to any concentration of ZEN. Our data suggest that ZEN induces DNA damage in Chang liver cells, and that the antioxidant activity of NACA may contribute to the reduction of ZEN-induced DNA damage and cytotoxicity via elimination of oxidative stress.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.97-104
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• 2005

This experiment was conducted to introduce strip croppings fur decreasing the damage of the continuous com cultivation at the experimental field of Grassland and Forage Crops division, National Livestock Research Institute from 1999 to 2000. Introduced mps for strip cropping to the continuously com cultivated field were red clover (RC), alfalfa(AL), and mixture of grass md legume. Fall panicum(FP), which is one of warm season's weeds was focused on its emerged quantity in com field changed by the level of soil water content and degree of the continuous corn cultivation. The ranges of yearly gained forage fresh yields including weeds like mainly FP were 119.4 ${\~}$ 169.6 in the continuous cultivation with com and rye and 64.9 ${\~}$ 83.3 ton / ha in the corn mono-cropping. Meanwhile, the percentages of pure com in continuous com cultivation plot were only $31-38\%$. This was for the mainly emergence of FP in the continuously com cultivated field. The ranges of average fresh yield of emerging FP were 45.6${\~}$63.4 ton/ ha in the continuously com cultivated field which were over the ranges of yields of rye, 28.2 ${\~}$ 41.6 ton/ ha. The dry matter yields of red clover, alfalfa and mixture with grass and legume introduced for strip cropping were 10.2, 10.6 and 10.6 ton / ha, respectively and these were about the 1/4 average fresh com yield. After strip cropping of red clover, alfalfa and mixture with grass and legume, the yields of corn increased to 56, 82, 88 and 79 ton / ha, respectively and com yield was much better in the alfalfa strip cropping.

• Toxicological Research
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• v.25 no.4
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• pp.243-251
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• 2009

Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.1
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• pp.71-81
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• 2020

Enclosed pig house are creating an environment with high concentrations of gas and dust. Poor conditions in pig farms reduce pig weight and increase disease and accidents for livestock workers. In the pig house, the high concentration of harmful gas may cause asphyxiation accidents to workers and chronic respiratory disease by long-term exposure. As pig farm workers have been aging and feminized, the damage to the health of the harsh environment is getting serious, and real-time monitoring is needed to prevent the damage. However, most of the measuring devices related to humidity, harmful gas, and fine dust except temperature sensors are exposed to high concentrations of gas and dust inside pig house and are difficult to withstand for a long time. The purpose of this study is to develop an wearable based device to monitor the hazardous environment exposed to workers working in pig farms. Based on the field monitoring and previous researches, the measurement range and basic specifications of the equipment were selected, and wearable based device was designed in terms of utilization, economic efficiency, size and communication performance. Selected H₂S and NH₃ sensors showed the average error of 5.3% comparing to standard gas concentrations. The measured data can be used to manage the working environment according to the worker's location and to obtain basic data for work safety warning.

• Journal of Korea Multimedia Society
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• v.17 no.7
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• pp.886-894
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• 2014

Recently, domestic animal disease caused tremendous damage to farmhouses and the damage stretched in nationwide with the spread of epidemic disease. To prevent animal diseases from happening again, the system development to easily measure the temperature of sick animals and identify of them is needed, thereby quickly treat them, reducing losses of farmhouses. However, a lack of related equipment and human resource hampered its effort to minimize its losses. This study tries to develop diagnosis system as part of measures to curb these domestic animal diseases. This paper present the 센서 node based on IEEE 802.15.4 which can be attached to the animal body for real-time temperature measurement. We design and implement tiny chip-type that can be attached to the body of animals. Then, we use available power only when measuring temperatures in a long term-basis. In this paper, the 센서 node was applied to horse's neck. We measure the horse's body temperature between $32.2^{\circ}C{\sim}33.7^{\circ}C$ and analyze phenomenon data for 4 months.

• Proceedings of the Korean Society for Rock Mechanics Conference
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• 2000.09a
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• pp.241-246
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• 2000

Blasting vibrations velocity had been used to design blasting pattern and predict damage-possibility of structures. As vibration levels are currently required to estimate the damage concerning human-body and livestock, so measured vibration velocities must be transformed into vibration levels. In this study, the digital filter program was developed to transform measured data into vibration levels. Simple harmonic vibrations and measured data obtained by Blastmate series (Instantel Inc.) were applied to the digital filter program. As a result, simple harmonic vibrations and measured data were successfully transformed into vibration levels using the developed program. Measured data from any instrument with ASCII output-function will similarly be transformed into level units.