• 한국동물생명공학회지
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• 제35권1호
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• pp.102-111
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• 2020

Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

• 한국동물위생학회지
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• 제38권4호
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• pp.253-257
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• 2015

Bull-boxing in South Korea is a form of competitions in which bulls measure their strength with each other in the form of pushing by putting their heads together. Bull-boxing has been admitted after being designated as a play culture by the government because it does not cause great injuries to animals' bodies and has strong positive aspects such as contributing to livestock farmers' leisure activities, encouragement of animal husbandry, and communities' economic development. However, bull-boxing sometimes causes damage to the head and horns due to the heavy bodies. According to the results of examinations of these damage cases, damage that caused irregular shapes of the base of horns was identified in 31.5% of fighting bulls. The damage to horns is thought to have been caused by the great forces of fighting bulls repeatedly imposed to each other's horns during bull-boxing that caused minute fractures leading to damage to the blood vessels inside the horns resulting in the blocking of delivery of nutrients to the horns causing the deformation of the horns into abnormal shapes. Since bulls' bodies are injured during bull-boxing although the injuries are small as bull-boxing is mainly conducted by pushing, animal protection groups regard bull-boxing as cruelty to animals and request to stop bull-boxing. The present study aims to develop animal welfare type headgears that can protect bulls' horns and heads in order to protect fighting bulls during bull-boxing in terms of animal protection and welfare.

• 한국농공학회논문집
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• 제56권1호
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• pp.41-49
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• 2014

Pig chronic wasting disease, including porcine reproductive and respiratory syndrome (PRRS) and postweaning multisystemic wasting syndrome (PMWS), have made a continuous economic damage in pig farms. Airborne spread of livestock viruses are an important spread factor which is difficult to analyze due to invisible airflow and limitation of measurement. The objective of this study is to analyze airborne disease spread between buildings in the experimental pig farm by means of field experiment and computational fluid dynamics (CFD). The field experiments were conducted to capture airborne virus using air sampler and teflon filter along multi points in the experimental pig farm. The samples were tested in terms of virus detection resulting in positive reaction for PRRS and PCV-2 viruses, which can be a firm evidence of airborne virus spread. The CFD simulation model was developed by considering complex topography, wind conditions, building arrangement, and ventilation systems and was used to analyze airborne virus spread according to different wind conditions. The CFD computed result showed a possibility of airborne virus spread via livestock aerosol from infected pig house to neighboring pig houses according to wind directions. The CFD simulation technique is expected to provide significant data for estimating and making a counterplan against airborne disease spread.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권4호
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• pp.1481-1498
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• 2014

Automated activity monitoring has become important in many applications. In particular, automated monitoring is an important issue in large-scale management of group-housed livestock because it can save a significant part of farm workers' time or minimize the damage caused by livestock problems. In this paper, we propose an automated solution for measuring the daily-life activities of pigs by using video data in order to manage the group-housed pigs. Especially, we focus on the circadian rhythm of group-housed pigs under windowless and 24-hour light-on conditions. Also, we derive a cost-effective solution within the acceptable range of quality for the activity monitoring application. From the experimental results with the video monitoring data obtained from two pig farms, we believe our method based on circadian rhythm can be applied for detecting management problems of group-housed pigs in a cost-effective way.

• Toxicological Research
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• 제29권1호
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• pp.43-52
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• 2013

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium that are found in cereals and agricultural products. ZEN has been implicated in mycotoxicosis in farm animals and in humans. The toxic effects of ZEN are well known, but the ability of an alkaline Comet assay to assess ZEN-induced oxidative DNA damage in Chang liver cells has not been established. The first aim of this study was to evaluate the Comet assay for the determination of cytotoxicity and extent of DNA damage induced by ZEN toxin, and the second aim was to investigate the ability of N-acetylcysteine amide (NACA) to protect cells from ZEN-induced toxicity. In the Comet assay, DNA damage was assessed by quantifying the tail extent moment (TEM; arbitrary unit) and tail length (TL; arbitrary unit), which are used as indicators of DNA strand breaks in SCGE. The cytotoxic effects of ZEN in Chang liver cells were mediated by inhibition of cell proliferation and induction of oxidative DNA damage. Increasing the concentration of ZEN increased the extent of DNA damage. The extent of DNA migration, and percentage of cells with tails were significantly increased in a concentration-dependent manner following treatment with ZEN toxin (p < 0.05). Treatment with a low concentration of ZEN toxin (25 ${\mu}M$) induced a relatively low level of DNA damage, compared to treatment of cells with a high concentration of ZEN toxin (250 ${\mu}M$). Oxidative DNA damage appeared to be a key determinant of ZEN-induced toxicity in Chang liver cells. Significant reductions in cytolethality and oxidative DNA damage were observed when cells were pretreated with NACA prior to exposure to any concentration of ZEN. Our data suggest that ZEN induces DNA damage in Chang liver cells, and that the antioxidant activity of NACA may contribute to the reduction of ZEN-induced DNA damage and cytotoxicity via elimination of oxidative stress.

• 한국초지조사료학회지
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• 제25권2호
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• pp.97-104
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• 2005

옥수수연작 피해방지를 위해 레드클로버 등을 대상작으로 도입하여 그 효과를 기대하였다. 특히 옥수수 연작지에 발생하는 난지형 잡초인 Fall panicum(FP)를 대상으로 옥수수/호밀연작 정도와 토양 습윤 정도에 따른 발생량을 조사하였다. 옥수수 재배와 동시에 대상 경작한 작물은 레드클로버(RC), 알팔파(A), 목초혼파(G/ R)이었다. 2년 동안 대상작물 재배 후 마지막 3년차에 옥수수 재배로 다시 환원하였는데 옥수수 연작지와 옥수수 단작에서의 얻어진 평균 생초수량의 범위는 각각 119.4${\~}$169,6 및 64.9${\~}$83.3톤/ha 이었으나 옥수수만의 수량에 있어서는 각각 45.6${\~}$52.8 및 64.9${\~}$83.3톤/ha로 서로 다른 결과를 얻었다. 이 같은 수량차이의 대부분은 옥수수 연작지에서의 FP의 발생량에 기인한 것으로 파악되었는데 실제 옥수수 연작지에서의 FP 발생량의 범위는 평균 45.6${\~}$63.4에 달하여 연작지에서의 호밀 28.2${\~}$41.6을 초과하였다. 대상작으로 도입한 레드클로버, 알팔파 및 목초 혼파의 3년간 건물 생산량은 각각, 10.2, 10.6 및 10.6톤/ha로. 연작구 평균 옥수수 수량의 대략 4분의 1정도인 것으로 나타났는데 대상작 도입 후 옥수수 생초 수량은 연작구 및 레드클로버, 알팔파 및 목초 혼파 대상작 도입구에서 각각 56, 82, 88 및 79톤/ha 로 대상작 도입구에서 크게 증가하였고 알팔파 도입 시 가장 크게 증가하는 것으로 나타났다.

• Toxicological Research
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• 제25권4호
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• pp.243-251
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• 2009

Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.

• 한국농공학회논문집
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• 제62권1호
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• pp.71-81
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• 2020

Enclosed pig house are creating an environment with high concentrations of gas and dust. Poor conditions in pig farms reduce pig weight and increase disease and accidents for livestock workers. In the pig house, the high concentration of harmful gas may cause asphyxiation accidents to workers and chronic respiratory disease by long-term exposure. As pig farm workers have been aging and feminized, the damage to the health of the harsh environment is getting serious, and real-time monitoring is needed to prevent the damage. However, most of the measuring devices related to humidity, harmful gas, and fine dust except temperature sensors are exposed to high concentrations of gas and dust inside pig house and are difficult to withstand for a long time. The purpose of this study is to develop an wearable based device to monitor the hazardous environment exposed to workers working in pig farms. Based on the field monitoring and previous researches, the measurement range and basic specifications of the equipment were selected, and wearable based device was designed in terms of utilization, economic efficiency, size and communication performance. Selected H₂S and NH₃ sensors showed the average error of 5.3% comparing to standard gas concentrations. The measured data can be used to manage the working environment according to the worker's location and to obtain basic data for work safety warning.

• 한국멀티미디어학회논문지
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• 제17권7호
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• pp.886-894
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• 2014

Recently, domestic animal disease caused tremendous damage to farmhouses and the damage stretched in nationwide with the spread of epidemic disease. To prevent animal diseases from happening again, the system development to easily measure the temperature of sick animals and identify of them is needed, thereby quickly treat them, reducing losses of farmhouses. However, a lack of related equipment and human resource hampered its effort to minimize its losses. This study tries to develop diagnosis system as part of measures to curb these domestic animal diseases. This paper present the 센서 node based on IEEE 802.15.4 which can be attached to the animal body for real-time temperature measurement. We design and implement tiny chip-type that can be attached to the body of animals. Then, we use available power only when measuring temperatures in a long term-basis. In this paper, the 센서 node was applied to horse's neck. We measure the horse's body temperature between $32.2^{\circ}C{\sim}33.7^{\circ}C$ and analyze phenomenon data for 4 months.

• 한국암반공학회:학술대회논문집
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• 한국암반공학회 2000년도 암반공학문제의 수치해석(Numerical Analysis in Rock Engineering Problems)
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• pp.241-246
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• 2000

발파 진동은 보통 지반진동속도로 계측되며, 발파설계나 구조물의 피해 산정에 적절하게 사용된다. 그러나 발파 진동에 의한 인체의 반응이나 가축의 피해 산정 등의 문제에서는 현행 소음진동규제법에서 명시하는 바, 인체의 감각적 감응척도에 충실한 진동레벨을 주로 사용하므로 변환문제가 발생한다. 본 연구에서는 발파 진동 계측기기로 계측한 데이터를 디지털 필터로 처리하여, 진동레벨로 변환하는 프로그램을 개발하고, 이를 단순조화진동과 실제 발파계측결과에 적용하였다. 그 결과, 단순조화진동은 물론이고 국내에서 널리 사용되고 있는 Instantel Inc.의 Blastmate series에서 계측된 진동데이터도 정밀도 높은 진동레벨로 변환이 가능하였다. 비슷한 정밀도로 계측결과를 ASCII file로 송출할 수 있는 기종에서 계측된 자료도 같은 정도로 처리할 수 있을 것으로 판정되었다