• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.142-148
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• 2002

Although ionizing radiation (IR) has been used to treat the various human cancers, IR is cytotoxic not only to cancer cells but to the adjacent normal tissue. Since normal tissue complications are the limiting factor of cancer radiotherapy, one of the major concerns of IR therapy is to maximize the cancer cell killing and to minimize the toxic side effects on the adjacent normal tissue. As an attempt to develop a method to monitor the degree of radiation exposure to normal tissues during radiotherapy, we investigated the transcriptional responses of human peripheral blood lymphocytes (PBL) following IR using cDNA microarray chip containing 1,221 (1.2 K) known genes. Since conventional radiotherapy is delivered at about 24 h intervals at 180 to 300 cGy/day, we analyzed the transcriptional responses ex-vivo irradiated human PBL at 200 cGy for 24 h-period. We observed and report on 1) a group of genes transiently induced early after IR at 2 h, 2) of genes induced after IR at 6 h, 3) of genes induced after IR at 24 h and on 4) a group of genes whose expression patters were not changed after IR. Since Biological consequences of IR involve generation of various reactive oxygen species (ROS) and thus oxidative stress induced by the ROS is known to damage normal tissues during radiotherapy, we further tested the temporal expression profiles of genes involved in ROS modulation by RT-PCR. Specific changes of 6 antioxidant genes were identified in irradiated PBL among 9 genes tested. Our results suggest the potential of monitoring post-radiotherapy changes in temporal expression profiles of a specific set of genes as a measure of radiation effects on normal tissues. This type of approach should yield more useful information when validated in in vivo irradiated PBL from the cancer patients.

• Journal of the Korea Convergence Society
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• v.4 no.2
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• pp.29-33
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• 2013

From one elementary school of last 2005 Pusan was started "the school police" system is being applied the effect in the elementary school of the human nature receiving entire country. But "with school police system " have a limit about manpower that is insufficient. So we try to make "Baumter-Gikimy " that is extended scollo police in seoul. Despite being like that at 2010 and 2011 cannot forget the elementary student suicide events which are shocking or sexual assault events occur from the campus.From elementary school 4 grade the students between junior high school 1 grade crime occurrence ratio are highest. These people the group leaving out mainly, suicide, with group violence and sexual assault problem gets up same problems. In order to solve like this problems stands the countermeasure of the dimension which is national certainly is necessary. But so far only the damage student and the parents have an interest in about like this problems and they hurt. We the smart phone of Android bases it will be able to solve like this problem points [ayp] from under planning and development boil the elementary student safe helper it will can use. When it uses the elementary student safe helper system which we propose before the accident occurs, it will grasp the state information of the student and it will be able to prevent an accident in advance. We will try to more efficient safe guard to elementary school student by real-time monitoring skill soon.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1612-1612
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• 2001

The production of grain for export and domestic use is one of Australia's most important agricultural industries, and the NIR technique has been used extensively over many years for the routine monitoring of grain quality, particularly moisture and protein content. Because most Australian grain is intended for human food production, the determinants of grain quality for livestock feed, apart from protein, have been largely ignored. However the increasing use of grain for feeding to pigs, poultry, beef cattle and dairy cows has led to an important national research project entitled “Premium Grains for Livestock”. Two of the objectives of this project are to determine the compositional and functional characteristics of grains which influence their nutritional quality for the various classes of livestock, and to adopt rapid and objective analytical tests for these quality criteria. NIR has been used in this project firstly to identify a set of grain samples from a large population of breeders' lines which showed a wide spectral variation, and hence a potentially wide variation in nutritional value. The selected samples were not only subjected to an extensive array of chemical, physical and in vitro analyses, but also were grown out to produce sufficient quantities of grain to feed to animals in vivo studies. Additional grains were also strategically selected from farms in order to include the effect of weather damage, such as rain, drought and frost. In this study to date, NIR calibrations have been derived or attempted, on both ground and whole grains, for in vivo dry matter digestibility (DMD), pepsin-cellulase dry matter disappearance, protein, fat, acid detergent fibre, neutral detergent fibre, starch, in sacco DMD and in vitro assays to simulate starch digestion in the lumen and small intestine. Results so far indicate high calibration accuracy for chemical components (SECV 0.3 to 2.6%) and very promising statistics for in vivo DMD (SECV 1.8, $R^2$ 0.93, SD 7.0, range 61.9 to 92.3, n=60). There appears to be some potential for NIR to estimate some in vitro properties, depending upon the accuracy of reference methods and appropriate sample populations. Current work is in progress to extend the range of grains with in vivo DMD values (a very laborious and expensive process) and to increase the robustness of the various NIR calibrations, with the aim of implementing uniform testing procedures for nutritional value of grains throughout Australia.

• Journal of Conservation Science
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• v.26 no.3
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• pp.277-294
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• 2010

The Seosanmaaesamjonbulsang (National Treasure No. 84) consists of light gray and coarse to mediumgrained biotite granite with partly developed pegmatite and quartz vein. The host rock is divided into dozens of rock blocks with various shape along irregular discontinuity plane. The evaluation results of discontinuity systems reveal that the host rock were exposed to instable sloping environments. Results of deterioration diagnosis show that the degree of damage has been made worse by physical weathering and surface discoloration laying stress on part that vertical and horizontal joints are massed. Generally, deterioration rate of the triad Buddha surface cover with 42.7%, however, the rate of physical weathering and surface discoloration are subdivided to 9.6% and 33.1%, respectively. Ultrasonic measurements indicate that the triad Buddha was reached highly weathered grade in general. And the rock material was weaken to show low velocity zone of 1,000m/s along irregular joint systems. Indoor and outdoor mean relative humidity of the shelter was recorded more than 70% during every season, and high frequency appears in high relative humidity range over 95%. Such environments seem to have produced dew condensation on the rock surface with rainfall and supply water, promoted physical, chemical and biological weathering along crack and joint, resulting in high permeation of water and percentage of water content. Therefore, it is judged that for scientific conservation of the triad Buddha it needs environment control through persistent preservation environment monitoring including water problem.

• Journal of Bio-Environment Control
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• v.29 no.1
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• pp.89-95
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• 2020

Livestock facilities in Korea are on their way of enlargement resulting in increment of livestock manure. When the livestock manure treated inappropriate way during application to the agricultural area, environmental damage can be occurred such as bad smell, water and air pollution. Therefore it is important to make a good management plan for livestock manure treatment. In order to effectively apply organic fertilizer made by livestock manure in terms of quantity and quality, the current status of nutrient in agricultural land should be calculated quantitatively by scientific way. The OECD suggest member countries to calculate the nutrient balance as an agricultural environment indicator which represents the difference between nutrient inputs and outputs of the amount of nutrients in various ways. In this paper, we calculated the nutrient balances according to three cities which have different characteristics such as urban or rural areas. To reduce the nutrient balance, we considered how to reduce the amount of chemical fertilizer by using organic fertilizer made by livestock manure. And public treatment facilities for livestock manure can be a good solution except civil complaints for location selection. Among the options, manure fertilizer application to the agricultural area for cultivation of forage crops was mainly considered to reduce the nutrient balance by decreasing input of chemical fertilizers and increasing output of crop production. Using the field monitoring data, it can be calculated that the daily nitrogen reductions by 116.5 kg/ha and the daily phosphorus increments by 2.7 kg/ha.

• Toxicological Research
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• v.25 no.2
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• pp.71-78
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• 2009

We investigated the pathogenesis of liver tissue damage during the lipid peroxidation and fibrogenesis with the observation of correlations between the parameters of collagen synthesis (and deposition) and lipid peroxidation in liver fibrosis (cirrhosis) rats. Rats were randomly divided into two groups, normal and $CCl_4$-thiopental sod. intoxicated group. And the one group was treated intragastrically with the mixture of $CCl_4$-thiopental sod. 3 times per week for 3 weeks. The liver tissue and sera were used for the measurement of hydroxyproline (HYP), malonedialdehyde (MDA) and superoxide dismutase (SOD). Biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-bilirubin and blood urea nitrogen (BUN) were measured. Additionally, the expression of collagen ${\alpha}1$(III) and $\beta$-actin mRNA was observed by RTPCR. The histological change in liver tissue was also observed by Masson's trichrome and H&E staining. Correlation analysis was carried by Spearman's rho method. All biochemical parameters except total-bilirubin were significantly higher in the $CCl_4$-thiopental sod. treated group than that of the normal group (p < 0.01). In the $CCl_4$-thiopental sod. treated group, Hyp as a parameter of collagen synthesis (deposition) and MDA as a metabolite of lipid peroxidation, were significantly elevated by 1.98 and 2.11 times higher than that of the normal group (p < 0.001) respectively. The activity of SOD in the $CCl_4$-thiopental sod. treated group is decreased significantly by 44.8% (p < 0.001). And collagen ${\alpha}1$(III) mRNA was more expressed in the $CCl_4$-thiopental sod. treated group than that of the normal group. However, the expression of $\beta$-actin mRNA is showed similar in both of groups. A good correlation was observed between the content of hyp and MDA concentration (r = 0.70, n = 40) in the two groups. And the correlation between the levels of hyp and SOD (r = -0.71, n = 25) is also reliable. However, no correlation were observed between MDA concentration and SOD (r = -0.40, n = 25) in the two groups. Elevated levels of MDA in $CCl_4$-thiopental sod. treated rats indicated enhancement of lipid peroxidation, which is accompanied by a decrease in SOD activity. Moreover, we could confirm that the parameters of collagen synthesis (and deposition) is in good correlation with the metabolite of lipid peroxidation (MDA) and the lipid peroxidation antagonizing enzyme (SOD). Hence, we propose that (1) lipid peroxidation and collagen synthesis (and deposition) could be enhanced by intragastrically application of $CCl_4$-thiopental sod. during a short terms. And (2) the intoxication of $CCl_4$-thiopental sod. could be used for monitoring of lipid peroxidation and collagen synthesis (and deposition) for test of antioxidant and antifibrotic agent.

• Journal of Conservation Science
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• v.32 no.3
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• pp.395-402
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• 2016

The impact of a large water control project on surrounding environment(temperature and relative humidity, precipitation, wind speed, present weather and visibility) was monitored. The survey have targeted on Silleuksa temple which is located in the waterside. The number of foggy days emerged as an environmental factor that can cause damage to cultural heritage. Under construction of weir since 2011, monthly relative humidity of Yeoju and Icheon was dropped to 9.6%. It depends on decrease in the number of raining days and precipitation of each year. Silleuksa temple is contiguous to Namhan River and only 100 m away from the waterside. Average wind speed is 0.5 m/s. Silleuksa has a site environmental factors which is often foggy. The number of foggy days of Silleuksa temple declined to 53 days soon after weir's completion. In case of fog, relative humidity of outside was higher than that of outside and inside of Josadang Shrine. Relative humidity difference of outside and inside of Josadang Shrine was 5.4% on average. Relative humidity of the number of foggy days is 6.3% higher on average than that of fine days. The width and dimensions of Namhan River increased by 1.45 times after weir's construction. It can change the number of foggy days. A long-term monitoring is positively necessary on fog occurrence and relative humidity.

• Journal of Conservation Science
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• v.35 no.6
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• pp.588-599
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• 2019

Conservation measures for excavated and surveyed buried cultural heritages are decided by the administrator of the Cultural Heritage Administration according to their scientific and historic values. However, management, preservation, and use after relocation and restoration remain unsystematic. An issue regarding preservation plan and use has arisen due to the damage of preserved heritages and protective facilities. Thus, this study aims to suggest a plan for preservation and use by investigating the present conditions of the preserved heritages in the Chungbuk area. Results showed that there are 43 preserved heritages in Chungbuk, most of which remain unmanaged systematically irrespective of the managing body. Remainder and protective facilities have been damaged due to wrong preservation treatments and selection of materials, and a problem also rises in terms of utilization, such as exhibition, education, and experience of preserved heritages. To improve such problems, a medium and long-term plan shall be established for the improvement of legal and institutional instruments, securing of budget, increase in professional manpower, development of standardization and inspection manuals, continuous monitoring, preventive preservation, research on relocation methods and materials, listing, and the creation of specialized museum and complex theme park. If a preservation plan suited to the investigated heritages can be developed, heritages can be preserved and managed more systematically and scientifically, and be used for various purposes like education, exhibition, public relations, etc.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.644-653
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• 2018

Landslides occur frequently due to the effects of heavy rainfall and typhoons caused by climate change. Erosion control measures are needed to effectively prevent landslide damage. In order to improve their efficiency, it is necessary to quantitatively measure the sediment discharge from the mountain stream. In this study, a load cell sensor was installed in a mountain stream and the measured values were compared according to the applicability and load test type in the mountain stream. The result of the load test showed that the effect of the loading type (load test 1, 2) was low at average (loadings) of 0.4kgf and 0.6kgf at sites 1 and 2, respectively. The load factor was also derived by regression analysis to increase the accuracy of the measured values. According to the results of the load factor (normalized) to the load-cell measurement value, the output value increased by 14.8% and 24.6% in sites 1 and 2, respectively, and was calculated to be similar to the reference value. The load cell sensor enabled us to quantitatively estimate the amount of sediment discharge in the mountain stream through time series analysis with the water level and rainfall information. If the monitoring is carried out for a long time, it can be used to find the sediment discharge mechanism for the mountain stream. In addition, applying sensors such as load-cells to a mountain stream is expected to contribute to the development of related industries, such as the manufacturing of measurement sensors.

• Journal of Korean Neurosurgical Society
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• v.29 no.10
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• pp.1289-1295
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• 2000

Purpose : Recent evidence suggests a possible role for leukocytes in brain injury following ischemia and reperfusion. This study examined the temporal profile of ischemic tissue damage and leukocyte response after transient middle cerebral artery occlusion(MCAO) with reperfusion in the mouse. Methods : Focal cerebral ischemia was made by temporary occluding of the stem of the proximal MCA. Two groups of the mouse were investigated : (1) sham operation(n＝10), and (2)those having the arterial occlusion released after 90 minute(n＝20). By 4 hours(n＝10) and 24 hours(n＝10) after the onset of ischemia-reperfusion, fluorescein videoimages were under-taken in the pial venules of the mouse using a closed cranial window technique. Rhodamine 6G was administered as a $80-100{\mu}l/min$ i.v. loading dose and a $30-40{\mu}l/min$ i.v. maintenance dose in saline to selectively label circulating leukocytes. Neuropathologic evaluation for brain injury was accomplished using the histochemical stain 2,3,5-triphen-yltetrazolium chloride(TTC) and hematoxylin and eosin(H & E) stain. Results : The mean number of adherent leukocytes to cerebral venules in the 90 minutes MCAO and 24 hours reperfusion group were $306{\pm}24$ compared with $72{\pm}8$ in the sham operation group. In the TTC staining method, the cortical infarct affecting 34.8% of hemispheric volume were created in all of animals (n＝10) undergoing 90 minute MCAO with 24 hours reperfusion, but the infarcted area were not found in the other(sham operation and 90 minute MCAO with 4 hours reperfusion)groups. In the H & E stain, the brain tissue following 90 minute MCAO with 4 hours reperfusion revealed only a pyknosis of the nuclei with shrunken cytoplasm, but infiltrated leukocytes were not observed. After 24 hours of reperfusion, a many leukocytes were infiltrated within parenchyma and blood vessles. Conclusions : These findings demonstrate the feasiblity of continous in vivo monitoring of leukocyte adherence in cerebral venules and suggest that reperfusion induced leukocyte adherence to venular endothelium may contribute to tissue injury following focal cerebral ischemia.