• Title/Summary/Keyword: damage control

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Transgenic Plants with Enhanced Tolerance to Environmental Stress by Metabolic Engineering of Antioxidative Mechanism in Chloroplasts (엽록체 항산화기구 대사조절에 의한 환경스트레스 내성 식물)

  • Kwon Suk-Yoon;Lee Young-Pyo;Lim Soon;Lee Haeng-Soon;Kwak Sang-Soo
    • Journal of Plant Biotechnology
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    • v.32 no.3
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    • pp.151-159
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    • 2005
  • Injury caused by reactive oxygen species (ROS), known as oxidative stress, is one of the major damaging factors in plants exposed to environmental stress. Chloroplasts are specially sensitive to damage by ROS because electrons that escape from the photosynthetic electron transfer system are able to react with relatively high concentration of $O_2$ in chloroplasts. To cope with oxidative stress, plants have evolved an efficient ROS-scavenging enzymes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX), and low molecular weight antioxidants including ascorbate, glutathione and phenolic compounds. To maintain the productivity of plants under the stress condition, it is possible to fortify the antioxidative mechanisms in the chloroplasts by manipulating the antioxidation genes. A powerful gene expression system with an appropriate promoter is key requisite for excellent stress-tolerant plants. We developed a strong oxidative stress-inducible peroxidase (SWPA2) promoter from cultured cells of sweetpotato (Ipomoea batatas) as an industrial platform technology to develop transgenic plants with enhanced tolerance to environmental stress. Recently, in order to develop transgenic sweetpotato (tv. Yulmi) and potato (Solanum tuberosum L. cv. Atlantic and Superior) plants with enhanced tolerance to multiple stress, the genes of both CuZnSOD and APX were expressed in chloroplasts under the control of an SWPA2 promoter (referred to SSA plants). As expected, SSA sweetpotato and potato plants showed enhanced tolerance to methyl viologen-mediated oxidative stress. In addition, SSA plants showed enhanced tolerance to multiple stresses such as temperature stress, drought and sulphur dioxide. Our results strongly suggested that the rational manipulation of antioxidative mechanism in chloroplasts will be applicable to the development of all plant species with enhanced tolerance to multiple environmental stresses to contribute in solving the global food and environmental problems in the 21st century.

Effect of Seeding Methods to the Growing Alopecurus aequalis var. amurensis in Wheat Field of Rice-wheat Cropping System (이모작 논 밀 재배시 파종방법이 뚝새풀의 생장에 미치는 영향)

  • Kim, Sun;Ahn, Seung-Hyeon;Im, Il-Bin;Cheong, Young-Keun;Kim, Si-Ju
    • Korean Journal of Weed Science
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    • v.30 no.3
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    • pp.252-257
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    • 2010
  • The experiments were carried out to develop ecological weed control system in wheat field of rice-wheat cropping system. The results were, depression effect on water foxtail by wheat seeding method decreased the seeding after doing soil rotary by 85% compared with the no practices. The occurrence of Water foxtail was 40 piece $m^{-2}$ on October 16, 29 piece $m^{-2}$ on October 26, and 4 piece $m^{-2}$ when surveying based on the standard of seeding the wheat at 15kg $ha^{-1}$ which decreased as the seeding time got later. According to the wheat seeding quantity 29 piece $m^{-2}$ occurred at 150kg $ha^{-1}$, and 8-11 piece $m^{-2}$ decreasingly at more than 200 kg $ha^{-1}$ of wheat seedlings based on seeding on October 26. As a result of summarizing the above results, crushing the soil by use of rotary before seeding wheat against the end of October, and seeding by increasing the seedling quantity (200 kg $ha^{-1}$) it is judged that the competition damage by weeds including water foxtail can be reduced without any use of herbicide.

Establishment of Rice Bakanae Disease Management Using Slightly Acidic Hypochlorous Acid Water (미산성 차아염소산수를 이용한 벼키다리 병 방제)

  • Goo, Sung-Geun;Koo, Jachoon
    • Journal of Life Science
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    • v.30 no.2
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    • pp.178-185
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    • 2020
  • Rice bakanae is a typical seed-borne and seed-transmitted disease caused by infection by Fusarium fujikuroi. Seed disinfection using chemical fungicides (such as benomyl and prochloraz) is most effective in controlling the disease, but the emergence of fungicide-resistant strains has recently been increasing. Slightly acidic hypochlorous acid water (SAHW) is a safe and environmentally friendly disinfectant that has a potent and broad spectrum of antimicrobial activity against viruses, bacteria, and fungi. In this study, we aimed to investigate the effectiveness of SAHW against F. fujikuroi strains, including chemical fungicide-resistant strains, as an alternative to conventional chemical fungicides in the management of bakanae disease. SAHW showed strong but similar levels of antifungal activity among the F. fujikuroi strains with a minimum inhibitory concentration (MIC90) of 5±2.5 ppm of free available chlorine (FAC). In addition, F. fujikuroi cells lost viability completely within 5 min of SAHW treatment due to the lethal damage to cell integrity. When the rice seeds infected by F. fujikuroi were treated with SAHW containing 20±10 ppm of FAC for 12 hr, the efficiencies of seed disinfection and disease control were 95-98% and 90.1-92.6%, respectively. Altogether, our data suggest that SAHW is an effective compound for controlling rice bakanae disease.

Fire Safety Analysis of Fire Suppression System for Aircraft Maintenance Hangar Using Fault Tree Method (Fault Tree를 활용한 항공기 격납고 소화시스템의 화재 안전성 분석)

  • Lee, Jong-Guk
    • Fire Science and Engineering
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    • v.31 no.6
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    • pp.67-73
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    • 2017
  • An aircraft maintenance hangar is a building that stores, maintains, and inspects expensive aircraft. The frequency of fire occurrence is low, but the resulting human and material damage can be very serious. Therefore, in this study, we conducted a qualitative analysis of the fire safety of the currently operating fire suppression systems for aircraft maintenance hangars using the Fault Tree method, and then performed a quantitative analysis using the failure rate data for the derived basic events and analyzed the importance of the minimal cut sets. As a result of the qualitative analysis by the minimal cut set, it was found that there were 14 accident paths that could be expanded to a large fire, due to the fire control failure of the aircraft hangar fire suppression system. The quantitative analysis revealed that, the probability of the fire expanding into a large one is $2.08{\times}E-05/day$. The analysis of the importance of the minimal cut set shows that four minimal cut sets, namely the fire detector and foam head action according to the zone and blocking of the foam by the aircraft wing and the fire plume, had the same likelihood of causing the fire to develop into a large one, viz. 24.95% each, which together forms the majority of the likelihood. It was confirmed for the first time by fault tree method that the fire suppression system of aircraft maintenance hangars is not suitable for fires under the aircraft wings and needs to be improved.

Screening of Antioxidative Activity of Hot-Water Extracts from Medicinal Plants (한약재 열수추출물의 항산화효과 검정)

  • Kang, Mi-Young;Nam, Seok-Hyun
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.141-147
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    • 2000
  • Interrelation between the antioxidative activity of hot-water extracts of 130 medicinal plants and their cellular antimutagenic activity was investigated. Antioxidative activity was evaluated by assaying electron-donation to DPPH free radical and scavenging of hydroxyl radical $({\cdot}OH)$ generated through Fenton rection, respectively. All medicinal plants examined in this study exhibited markedly electron-donating ability and radical scavenging ability in each assay system. The results demonstrated the fact that Pilbal (Piper longum L.) is the strongest in electron-donating activity, on the other hand, that Seokgok (Dendrobium moniliforme L.) is the strongest in ${\cdot}OH$ scavenging activity. When evaluated their antioxidative activities, 24 medicinal plants including Jimo (Anemarrhena asphodeloides Bunge) were found to be the medicinal plants carrying strong antioxidative activity, which exhibited more than 50% activity compared to the control group in both electron-donating and free radical scavenging. The experiment was also performed to examine whether 11 medicinal plants having significant antimutagenicity damage DNA in the presence of $Cu^{2+}$, showing the fact that all samples tested, except Taeksa (Alisma canaliculatum All. Br.), Paekjain (Nitraria sibirica Pall) and Ohyak (Lindera strychifolia Sieb. et Zucc. Villar) are capable of inducing DNA strand break. We also found that Taeksa and Paekjain strongly block DNA strand break induced by chemical mutagen mitomycin C.

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Protective Effect of Pesticide on Radiation-Induced Cell Damage in Tradescantia 4430 Stamen Hairs (자주달개비 수술털에서 방사선에 의해 유발되는 세포손상에 대한 살충제의 방어효과)

  • 김진규;김원록;이창주;장화형;이영근
    • Korean Journal of Environmental Biology
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    • v.17 no.1
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    • pp.21-26
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    • 1999
  • To investigate the combined effect of radiation and pesticide on Tradescantia somatic cell mutations, potted plants of Tradescantia 4430 on which parathion had been sprayed evenly 24 hours before irradiation. Radiation doses were 0.3, 0.5, 1.0 and 2.0 Gy of gamma-ray. The plants irradiated only with the gamma-ray radiation were used as control groups (CT). Pink mutation frequency increased linearly proportional to the radiation dose and the peak interval of elevated mutation frequencies appeared during 7 ~ 11 days after irradiation in both CT and Pa +${\gamma}$ groups. The slope of dose -response curve in CT was 5.99 ($r^2$= 0.988), while it was 3.43 (r$x^-2$=0.981) in Pa+${\gamma}$. It seemed that parathion pretreatment had a protective effect against radiation-induced cell damages since it decreased the slope value by 43%. It is suggested that an adaptive response or radiomodification could be induced in irradiated stamen hair cells by parathion pretreatment.

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The Development of Tobacco Litigation in USA and it's Impact of Law and Politics in Public Health (미국 담배소송의 변천과 보건법정책 효과)

  • Kim, Un-Mook;Kim, Ji-Hyun
    • The Korean Society of Law and Medicine
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    • v.12 no.1
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    • pp.133-173
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    • 2011
  • Since mid-1960s the reports from the Surgeon General, the World Health Organization, and other health experts state that there is no risk-free level exposure to smoking and secondhand smoke. Tobacco smoke is made up of more than 7,000 chemicals. Hundreds are toxic, and at least 70 are carcinogens. The chemicals in tobacco smoke reach smoker's lungs quickly every time smoker inhale causing damages immediately. Inhaling even the smallest amount of tobacco smoke can also damage smoker's DNA, which can lead to cancers. Smoking is responsible for more than 87% of lung cancers, but there are a host of other chronic diseases directly related to exposure to tobacco smoke. It's also a major cause of heart disease, stroke, aortic aneurysm, peripheral arterial disease and most of the other diseases. In the United States, each year with more than from 440,000 to 520,000 deaths caused by smoking and exposure to involuntary smoke. They conclude that smoking is the single most important source of preventable morbidity and mortality. The United States of America have about 60-year history of tobacco litigation. Tobacco litigation has been an important tool in tobacco control strategies aimed at limiting the activities of tobacco companies and providing redress to people who have become ill as a result of their use of tobacco products. Tobacco litigation is a kind of tort litigation. Quite often, as in the asbestos and other mass tort litigation episodes, tobacco litigation can play an educational role, warning the public about the magnitude of health risks that might otherwise be less clearly perceived. Tobacco litigation allows smokers, their families or other victims of smoking to sue tobacco companies in order to be compensated for the harm they have suffered. Potential benefits of tobacco litigation include compensation for smoking-related damages, strengthening regulatory activity, publicity, documents disclosure and changing tobacco industry behavior. And also tobacco litigation can limit the political activities of tobacco industry, protect human rights of smokers and non-smokers, increase burden to tobacco price-up and enhance the effects of law and politics in public health.

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Hepatoprotective effects of the aqueous extract from Taraxacum officinale (Dandelion) against Thioacetamide-induced hepatotoxicity in rats (Thioacetamide로 유발된 간 손상에 대한 Taraxacum officinale (Dandelion) 추출물의 효과)

  • Cho, In-Young;Ma, Se-Ryung;Moon, Sun-Jin;Yu, Do-Hyeon;Shin, Sung-Shik;Son, Chang-Ho;Oh, Ki-Seok;Hur, Tai-Young;Jung, Young-Hun;Choi, Chang-Yong;Suh, Guk-Hyun
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.233-242
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    • 2013
  • The genus Taraxacum, known by the common name Dandelion, is a medicinal herb in the family Asteraceae. It has been traditionally used as a folk medicine for the treatment or prevention of various diseases due to its anti-inflammatory and anti-oxidative properties. In this study, we attempted to evaluate protective effects of Dandelion related with anti-oxidative activity to Thioacetamide (TAA)-induced liver damage. 36 rats were randomly assigned to six experimental groups : Control, Dandelion water extract (DWE), TAA, TAA&DWE 300, TAA&DWE 600, TAA&DWE 1,200 groups. Rats in DWE and TAA&DWE groups were pretreated with DWE (300, 600 or 1,200 mg/kg BW) by gavage for 7 days. All rats were treated intraperitoneally with TAA (200 mg/kg BW) or normal saline at 12 hours after last oral administration and sacrificed at 12 hours after last treatment. Levels of WBC and Neutrophil count were significantly decresed in TAA&DWE 1,200 group compared with that in TAA group (P<0.05). In TAA&DWE 600 and TAA&DWE 1,200 groups, serum AST, ALT, GGT levels were lower than TAA group (P<0.05). The serum TG level was significantly elevated in TAA&DWE groups compared with those in TAA group. Liver tissues from TAA group showed extensive histopathological changes, characterized by moderate or severe hepatocytes degeneration, inflammatory cell infiltration, and congestion. In the TAA&DWE group, The severity of histopathological lesions were decreased compared to those in the TAA group. The MDA concentration was significantly decreased and GSH content was significantly increased in the TAA&DWE 1,200 group compared to those in the TAA group. GR, CAT and GST activities in the TAA&DWE 1,200 group were significantly increased compared to those in the TAA group.

A Study on Required Safe Egress Time (RSET) Comparison and Error Calculation in Relation to Fire Room Range Set Conditions of Performance Based Fire Safety Designers (성능위주설계자들의 화재실 범위 설정 방식에 따른 소요피난안전시간(RSET) 비교 및 오차산정에 관한 연구)

  • Baek, Sona;Choi, Jun-Ho;Hong, Won-Hwa;Jung, Jong-Jin
    • Fire Science and Engineering
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    • v.30 no.3
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    • pp.73-78
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    • 2016
  • The Installation, Maintence, and Safety Control of Fire-fighting Systems Act of South Korea regulates that over 30-storey high-rise buildings including underground spaces should vitally perform the Performance-based Design to minimize property damage and personal injury as a fire risk assessment in advance. Therefore a PBD designer such as a fire safety professional engineer evaluate occupant's life safety by a scientific methodology. In order to evaluate the life safety, fire safety designers calculate the Required Safety Egress Time (RSET) which does not have the legal criteria regarding the standard method of calculation yet. So this way has been showing different results depending upon the designer's choice, knowledges and experiences. In this study, RSET calculation methods by six designers respectively were analysed from the thirteen reports of real performance based design projects conducted in Busan for a last five years. In particular, the Response Time calculation methods which have the most powerful effect for figuring the RSET are compared with the other designer's to deduce an error value.

Survey on Nutrient Removal Potential and Growth State of Water Hyacinth (Eichhornia crassipes) at Seo-Ho. (서호수에서 부레옥잠 생육상태와 영양염류 제거량 조사)

  • Kim, Bok-Young;Lee, Jong-Sik;Kim, Jin-Ho
    • Korean Journal of Environmental Agriculture
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    • v.17 no.2
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    • pp.145-149
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    • 1998
  • To find out the effect of water hyacinth(Eichhornia crassipes Solms-Laub) on the control of eutrofication and plant damage with irrigation water contamination, its ability to remove the nutrients such as nitrogen and phosphrus was studied. The overall results are summarized as follow. 1. It was possible to cultivate water hyacinth for 4 month from June to the middle of October in Suwon. 2. The number of plant was increased from 1,320 to 50,600 for 4 months, and total amounts of N, P, K removed from Seo-Ho by water hyacinth were 78.3, 64.2 and 152.4 kg/10a, respectively. 3. EC and $NH_4-N$ contents were lower at water hyacinth treatment than non-treatment and monthly variation of those contents were decreased until August. 4. With the cultivation of water hyacinth in animal waste water in vinyl plastic house, pH, EC, COD and concentrations of $NH_4-N$ and $PO_4-P$ in the water were drastically decreased. 5. Nitrogen contents absorbed from waste water existed in the order of leaf > stem > root but those of phosphorus root > stem > leaf.

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