• BMB Reports
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• 제50권7호
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• pp.379-383
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• 2017

We previously reported that p53 plays a role as a key regulator in the tetraploid G1 checkpoint, which is activated by actin damage-induced cytokinesis blockade and then prevents uncoupled DNA replication and nuclear division without cytokinesis. In this study, we investigated a role of Skp2, which targets CDK2 inhibitor p27/Kip1, in actin damage-induced tetraploid G1 arrest. Expression of Skp2 was reduced, but p27/Kip1 was increased, after actin damage-induced cytokinesis blockade. The role of Skp2 repression in tetraploid G1 arrest was investigated by analyzing the effects of ectopic expression of Skp2. After actin damage, ectopic expression of Skp2 resulted in DNA synthesis and accumulation of multinucleated cells, and ultimately, induction of apoptosis. These results suggest that Skp2 repression is important for sustaining tetraploid G1 arrest after cytokinesis blockade and is required to prevent uncoupled DNA replication and nuclear division without cytokinesis.

• Composites Research
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• 제12권2호
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• pp.62-69
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• 1999

복합재료가 피로하중을 받으면 재료 내부에 손상이 누적되며, 이는 재료의 물성 변화로 나타난다. 본 연구에서는 손상을 나타내는 인자로 피로계수를 사용하였다. 피로계수와 참고계수로 정의되는 손상함수로부터 복합재료의 피로수명 예측을 이론적으로 연구하였다. 제안된 모델들은 인가 응력 수준, 피로주기 및 피로수명의 함수로 유도하였다. 예측 결과는 유리섬유/에폭시 복합재료와 유리섬유/폴리에스터 복합재료를 사용한 다중응력 피로 실험 데이터를 이용하여 검증하였다.

• 항공우주시스템공학회지
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• 제3권4호
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• pp.27-34
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• 2009

This paper deals with the lifetime prediction of Aircraft Flaperon Joint made of AISI 4130 steel. Reviews are performed on the published damage models at first. And three different damage models are used for predicting the fatigue life of the structure subjected to variable amplitude fatigue loading. These models require no increase in complexity of use, nor do they require additional material property or mission loading information to achieve the improved accuracy. Finally a comparison among the fatigue results is performed. It is observed that the Miner's rule could predict longer life than other cumulative damage models which take into account loads below the endurance limit.

• 한국측량학회지
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• 제24권1호
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• pp.37-45
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• 2006

여름철에는 집중강우에 의한 산사태, 하천범람, 하천구조물의 피해가 심각하다. 특히 토양침식의 증가는 하상퇴적과 저수량 저하 등의 홍수재해를 발생시키며, 상류에서의 침식증가는 토사의 하천범람, 농경지와 시가지 매몰의 형태로 나타난다. 현재까지의 피해현황 조사는 답사 위주의 조사가 주를 이루고 있어 효율적인 피해조사에 한계가 있으며, 따라서 효과적이며 과학적인 모델링 기법이 필요하다. 이 연구에서는 다양한 공간 해상도의 인공위성영상 분석 비교를 통해 고해상도 위성영상을 모니터링 자료원으로의 활용성을 검토하고자 하였으며, 토사재해(침식/퇴적) 피해 현황 정보의 추출 가능성을 파악하고자 하였다. 또한 위성영상의 해상도별 분석을 통하여 토사재해 피해현황판독 활용 가능성을 연구하였고, 하천 미세지형 변화의 판독을 위한 KOMPSAT 영상의 활용성을 연구하였다.

• Structural Engineering and Mechanics
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• 제17권5호
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• pp.655-670
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• 2004

This paper aims to evaluate the effect of typhoons on fatigue damage accumulation in steel decks of long-span suspension bridges. The strain-time histories at critical locations of deck sections of long-span bridges during different typhoons passing the bridge area are investigated by using on-line strain data acquired from the structural health monitoring system installed on the bridge. The fatigue damage models based on Miner's Law and Continuum Damage Mechanics (CDM) are applied to calculate the increment of fatigue damage due to the action of a typhoon. Accumulated fatigue damage during the typhoon is also calculated and compared between Miner's Law and the CDM method. It is found that for the Tsing Ma Bridge case, the stress spectrum generated by a typhoon is significantly different than that generated by normal traffic and its histogram shapes can be described approximately as a Rayleigh distribution. The influence of typhoon loading on accumulative fatigue damage is more significant than that due to normal traffic loading. The increment of fatigue damage generated by hourly stress spectrum for the maximum typhoon loading may be much greater than those for normal traffic loading. It is, therefore, concluded that it is necessary to evaluate typhoon induced fatigue damage for the purpose of accurately evaluating accumulative fatigue damage for long-span bridges located within typhoon prone regions.

• 한국세라믹학회지
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• 제35권7호
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• pp.671-678
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• 1998

Hertzian indentation tests with sphere indenters were used to study the mechanical properties of glass-in-filtrated alumina and spinel composites and evaluated the effect of preform microstructure and evaluated the effect of preform microstructure and glass con-tents on contanct damage and strength. The spinel composite showed more brittle behavior than the alumina composite which is verified from indentation stress-strain curve cone cracks and quasi-plastic deformation developed at subsurface. Failure originated from either cone cracks(brittle mode) or deformation zone(quasi-plastic mode) above critical load for cracking(Pc) and yield ({{{{ {P }_{Y } }}) with the brittle mode more dominant in the spinels and the quasi-plastic mode more dominant in the aluminas. Even though brittle mode was dominant in the spinel composites the strength degradation from accumulation of damage above these critical loads was conspicuously small suggesting that the glass-infiltrated composites should be highly damage tolerant to the blunt contacts.

• Structural Engineering and Mechanics
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• 제36권4호
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• pp.481-497
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• 2010

The shaking table tests were conducted on two small-scale models (Model 1 and Model 2) to examine the earthquake-induced damage of a concrete gravity dam, which has been planned for the construction with the recommendation of the peak ground acceleration of the maximum credible earthquake of 0.42 g. This study deals with the numerical simulation of shaking table tests for two smallscale dam models. The plastic damage constitutive model is used to simulate the crack/damage behavior of the bentonite-concrete mixture material. The numerical results of the maximum failure acceleration and the crack/damage propagation are compared with experimental results. Numerical results of Model 1 showed similar crack/damage propagation pattern with experimental results, while for Model 2 the similar pattern was obtained by considering the modulus of elasticity of the first and second natural frequencies. The crack/damage initiated at the changing point in the downstream side and then propagated toward the upstream side. Crack/damage accumulation occurred in the neck area at acceleration amplitudes of around 0.55 g~0.60 g and 0.65 g~0.675 g for Model 1 and Model 2, respectively.

• Biomolecules & Therapeutics
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• 제31권3호
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• pp.340-349
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• 2023

Mad2B (Mad2L2), the human homolog of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares sequence similarity with the mitotic checkpoint protein Mad2A. Previous studies on Mad2B have concluded that it is a mitotic checkpoint protein that functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C). Here, we demonstrate that Mad2B is activated in response to cisplatin-induced DNA damage. Mad2B co-localizes at nuclear foci with DNA damage markers, such as proliferating cell nuclear antigen and gamma histone H2AX (γ-H2AX), following cisplatin-induced DNA damage. However, unlike Mad2A, the binding of Mad2B to Cdc20 does not inhibit the activity of APC/C in vitro. In contrast to Mad2A, Mad2B does not localize to kinetochores or binds to Cdc20 in spindle assembly checkpoint-activated cells. Loss of the Mad2B protein leads to damaged nuclei following cisplatin-induced DNA damage. Mad2B/Rev7 depletion causes the accumulation of damaged nuclei, thereby accelerating apoptosis in human cancer cells in response to cisplatin-induced DNA damage. Therefore, our results suggest that Mad2B may be a critical modulator of DNA damage response.

• 대한의생명과학회지
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• 제17권3호
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• pp.239-243
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• 2011

Radiation exposure may cause tissue damage by ionized or excited atoms. The molecules are transformed from water or they directly interact with cells. Antioxidants such as vitamin E, vitamin C are used to reduce the interference of radiation. Especially, vitamin E inhibits the formation and accumulation of lipid and oxidation and the activity of drug-metabolizing enzymes. After irradiation of 3 Gy to the testis, the entire shape of testis was partly depressed and an irregular vacuole was observed to the edge. The 3 Gy group after treatment with vitamin E showed the low destruction of spermatogonia and partial tissue damage as compared to the 3 Gy group. Vitamin E inhibited the production of vacuoles from the cell death and generated the block to prevent the condensation of the seminiferous tubules.

• Earthquakes and Structures
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• 제7권6호
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• pp.937-953
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• 2014

A semi-active control platform comprising the mechanical model of magnetorheological (MR) dampers, the bang-bang control law and damage material models is developed, and the simulation method of steel plate shear wall (SPSW) and optimization method for capacity design of MR dampers are proposed. A 15-story steel frame-SPSW structure is analyzed to evaluate the seismic performance of nonlinear semi-active controlled structures with optimal designed MR dampers, results indicate that the control platform and simulation method are stable and fast, and the damage accumulation effects of uncontrolled structure are largely reduced, and the seismic performance of controlled structures has been improved.