• Molecules and Cells
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• 제37권1호
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• pp.51-57
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• 2014

NOG1 is a nucleolar GTPase that is critical for 60S ribosome biogenesis. Recently, NOG1 was identified as one of the downstream regulators of target of rapamycin (TOR) in yeast. It is reported that TOR is involved in regulating lifespan and fat storage in Caenorhabditis elegans. Here, we show that the nog1 ortholog (T07A9.9: nog-1) in C. elegans regulates growth, development, lifespan, and fat metabolism. A green fluorescence protein (GFP) promoter assay revealed ubiquitous expression of C. elegans nog-1 from the early embryonic to the adult stage. Furthermore, the GFP-tagged NOG-1 protein is localized to the nucleus, whereas the aberrant NOG-1 protein is concentrated in the nucleolus. Functional studies of NOG-1 in C. elegans further revealed that nog-1 knockdown resulted in smaller broodsize, slower growth, increased life span, and more fat storage. Moreover, nog-1 over-expression resulted in decreased life span. Taken together, our data suggest that nog-1 in C. elegans may be an important player in regulating life span and fat storage via the insulin/IGF pathway.

• 대한토목학회논문집
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• 제31권3A호
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• pp.163-172
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• 2011

CFTA 거더는 아치형상을 갖는 콘크리트 충전 강관구조이며 초기처짐 및 공용 중 응력제어를 위해 외부긴장재를 배치한 거더 형식이다. 본 연구에서는 차량진행에 따른 거더의 동적거동에 긴장재가 미치는 영향을 분석하였다. 이를 위해 유한요소 프로그램을 이용하여 거더 및 긴장재 등을 수치모델링하였으며 긴장재의 양과 긴장력을 다양한 값으로 변화시켰다. 차량하중은 도로교설계기준의 DB-24 하중을 고려하였으며 3축-2트랙으로 모델링하였다. 차량하중은 등가절점하중으로 적용시켰으며 차량하중의 이동은 차량통과 시간 및 절점수를 고려한 각 절점에서의 시간함수로 나타내었다. 차량속도는 40 km/hr에서 100 km/hr까지 20 km/hr씩 증가시켰다. 해석결과 긴장재의 긴장력 변화는 거더의 동적거동에 영향을 주지않았으며 초기처짐에만 영향을 주었다. 긴장재의 양에 따라서는 거더의 동적거동이 다르게 나타났으며 긴장재의 양이 적을수록 동적처짐은 증가하였다. 이를 바탕으로 거더의 동적증폭계수(DAF)를 산출하였으며 긴장재가 없는 경우에도 AASHTO LRFD와 도로교 표준시방서에서 정한 기준값보다 매우 작은 안정적인 거동을 보였다.

• 생명과학회지
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• 제9권6호
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• pp.692-697
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• 1999

주정폐액과 같은 고 농도의 유기성 폐액의 효율적 처리에는 혐기적, 호기적인 생물학적인 처리와, 생물학적으로 난분해성 물질의 화학적 처리가 요구되었다. 난분해성 COD성분을 제거하기 위하여 Fenton처리 전에 가능한 많은 유기물의 제거가 요구되며, 이때 $FeCl_3$,alum,cationic polymer의 처리가 효과적이었다. 이 처리과정 중은 몰론 Fenton 처리과정 중에 생성되는 슬러지의 제거 효율에 따라 전체 폐수처리효율리 크게 영향을 받게되 슬러지의 효과적인 제거과정이 필요하였다, 이를 위해 기포의 직경이 80-120 um 정도되는 기존의 DAF방식에 비해 새로 도입한 PBF법에 의해 생성되는 직경 20-50um 의 미세포기는 슬러지의 부상을 촉진시켜 화학적 슬러지의 제거 효율이 훨씬 뛰어남을 알 수 있었다. 이에 따라 PBF법에 의한 슬러지의 제거는 기존의 DAF법에 비해 약품 사용량, 용수의 절감, 방류량의 감소 등으로 전체 운전비용을 약 30%정도 줄일 수 있었다.

• 한국작물학회지
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• 제49권1호
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• pp.19-24
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• 2004

Germination and emergence habits of Chinese milk vetch (CMV) were examined to obtain the basic information for the effect of environmental conditions and cultural practices on the seedling density. Seed germination tests with different water potentials, temperatures, and soil and water depths showed the environmental effects on the characters related to seed germination in CMV. Imbibition under different temperatures reflects that initial velocity was rapid at higher temperature, however, the times to full imbibition were not different between 15 and $25^{\circ}$. The optimal germination temperature for CMV germination was ranged from 15 to 20 and the germination was highly affected by water potential of media at relatively high temperature above $20^{\circ}$. When the seeds were sown in flooded condition the germination was not proportionally affected by water depth. In addition, there was no correlation between water depth and oxygen concentration. The germination of seeds flooded by 2cm water depth were poorly germinated compared to other depths. Results indicated that the germination of submerged seeds was more highly influenced by flooding depth than the temperature, it was also affected more strongly at 10 than $20^{\circ}$. Emergence of CMV depending on the thickness of covered soil was poor when the soil layer was greater than 5cm. In the experiment with seeds collected between 22 days after flowering (OAF) and 52 DAF, the highest germination ability of CMV seeds was observed at 39 DAF and germinability was decreased subsequently as seeds became mature. The lower germinability may be due to the enhanced seed dormancy.

• ETRI Journal
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• 제40권3호
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• pp.291-302
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• 2018

The deployment of an incremental hybrid decode-amplify and forward relaying scheme is a promising and superior solution for cellular networks to meet ever-growing network traffic demands. However, the selection of a suitable relaying protocol based on the signal-to-noise ratio threshold is important in realizing an improved quality of service. In this paper, an incremental hybrid relaying protocol is proposed using polar codes. The proposed protocol achieves a better performance than existing turbo codes in terms of capacity. Simulation results show that the polar codes through an incremental hybrid decode-amplify-and-forward relay can provide a 38% gain when ${\gamma}_{th(1)}$ and ${\gamma}_{th(2)}$ are optimal. Further, the channel capacity is improved to 17.5 b/s/Hz and 23 b/s/Hz for $2{\times}2$ MIMO and $4{\times}4$ MIMO systems, respectively. Monte Carlo simulations are carried out to achieve the optimal solution.

• BMB Reports
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• 제50권4호
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• pp.160-161
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• 2017

Proper maintenance of biological components is crucial for longevity and healthy aging. Although the role of homeostatic maintenance systems for DNA and protein in longevity is established, it remains largely unknown for RNA. In our recent work, we show that nonsense-mediated mRNA decay (NMD) promotes longevity in the roundworm C. elegans by enhancing RNA quality control. We find that the activity of NMD decreases during aging, raising the possibility that RNA quality declines in old animals. We then show that key components of NMD complex are required for prolonged lifespan in C. elegans. In addition, animals with reduced insulin/insulin-like growth factor-1 (IGF-1) signaling (IIS), a representative longevity model, display increased NMD activity. Thus, up-regulation of NMD appears to play crucial roles in longevity conferred by reduced IIS via enhancing mRNA quality control. As both IIS and NMD pathways are evolutionarily conserved, mammals including humans may be equipped with similar RNA quality control systems to achieve longevity.

• 상하수도학회지
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• 제19권2호
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• pp.161-172
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• 2005

Recently, water treatment methods utilizing microbubbles such as DAF and EF are gaining interest and being studied. Current study is focused on the fundamental research of electroflotation by examining the characteristics of microbubbles and particles. The objects of this research consist of two things; (1) theoretical modeling of microbubble-particle collision, (2) the experimental investigation of removal efficiency of turbidity in electroflotation. From investigation, the mechanism of electroflotation can be explained not only by the characteristics of microbubbles and particles but also the chemistry of aluminum dissolved from aluminum electrode during the electroflotation experiment.

• 생약학회지
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• 제48권3호
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• pp.179-186
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• 2017

Pyrrosia lingua which belongs to Polypodiaceae has been used as a traditional medicine for the treatment of urinary system inflammation, urination disorder, and bronchitis. However, there are not enough phytochemical and pharmacological studies of P. lingua up to now. Here in this study, the protective effect of MeOH extract of whole plant of Pyrrosia lingua (MPL) against 2% glucose-induced toxicity was investigated using Caenorhabditis elegans (C. elegans) model system. We found that MPL significantly extended the lifespan of wild-type nematode under normal culture condition. MPL also effectively recovered the decreased lifespan caused by 2% glucose-toxicity. In addition, MPL efficiently attenuated the increased glucose concentration inside of nematode. Further studies evaluating diabetes-related factors revealed that MPL reduced both intracellular ROS and lipid accumulation which were up-regulated under 2% glucose supplement condition. Our data also showed that MPL improved the 2% glucose-induced shortened body movement of nematode. Lastly, we carried out genetic studies using several single gene knockout mutants to establish the possible target of MPL. Our results demonstrated that genes such as daf-2 and daf-16 were responsible for the protective activity of MPL against 2% glucose-induced toxicity. These results indicate that MPL exerts protective action against 2% glucose via regulation of insulin/IGF-1 sinaling pathway and FOXO activation.

• 한국시뮬레이션학회논문지
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• 제5권2호
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• pp.13-24
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• 1996

Workflow is an ordered sequence of interdependent component data activities each of which can be executed on an integrated information system by accessing a remote information system. In our previous research [4], we proposed a distributed CIM Workflow system which consists of a workflow execution model called DAF-Net and an agent-based information systems called AIMIS. Given a component data activity, there needs an interaction protocol among agents which allocates the component data activity to a relevant information systems exist. The objective of this research is to propose and test two protocols: ARR(Asynchronous Request and Response)protocol and NCL(Negotiation with Case based Learning) protocol. To test the effectiveness of the protocols, we applied the PVM(Parallel Virtual Machine) software to simulate the distributed CIM Workflow system. PVM provides a distributed computing environment in which users can run different software processes in different computers while allowing communication among the processes.

• International Journal of Oral Biology
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• 제33권2호
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• pp.65-70
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• 2008

Oral spirochetes are anaerobes known as one of causative agents for periodontal diseases. In this study, we investigated the possibility of utilizing fluorescent fatty acids for labeling oral spirochetes. Bacterial labeling was standardized with three different lengths of fluorescent fatty acids: 5-octadecanoylaminfluorescein (OAF), 5-dodecanoylamin-fluorescein (DAF), and 5-hexadecanoylaminfluorescein (HAF). Among these fatty acids, OAF showed the best labeling activity. Treponema denticola ATCC 35405 was totally saturated to the maximum when incubated with OAF $1\;{\mu}g/ml$ for 1 hour. Treponema vincentii LA-1 also increased in fluorescence in proportion to incubation time length and the concentration. In conclusion, these findings showed the possibility that the fluorescent fatty acid can be used for labeling oral spirochetes.