• 대한한의학회지
• /
• 제16권2호
• /
• pp.281-298
• /
• 1995

The following results were made by observation GOT, GPT, ALP, total cholesterol and higlyceride in serum to research the effects of medicines which are Injinsaryungsan(Sample- A) and another medicine(Sample-B) of which Injin(Artemisiae Capillaris Herba)was increased in quantity on liver damaged by $CCl_4$ and d-galactosamine in rats. 1. The high concentrated extracts of Sample A group and Sample- B group showed significant inhibltory effects on the increase of serum GPT, ALP, LDH, total cholesterol and triglyceride levels induced by $CCl_4$ and d-galactosamine. 2. The high concentrated extracts of sample A group and Sample B group showed more significant inhibitory effects(P〈0.001) than the low ones' effects(P〈0.01) on the increase of serum triglyceride level induced by $CCl_4$ 3. Sample-A group showed significant inhibitory effects on the increase of serum GOT, GPT. ALP, total cholesterol and triglyceride levels, but no significance on the increase of serum LDH level induced by d-galactosamine. 4. Sample-B group showed very significant inhibitory effects on the increase of serum GOP, GPT, ALP, LDH, total cholesterol and triglyceride levels induced by d-galactosamine. 5. As compared with Sample-A group, Sample-B group of which Injin was increased in quantity showed more significant inhibitory effects on all items of this experiment induced by $CCl_4$ and d-galactosamine. As mentioned above. it seemed that both Injinsaryungsan and another medicine of which Injin was increased in quantity had effects protecting liver and anti-fatty liver induced by $CCl_4$ and d-galactosamine in rats. Specially Sample-B group had very significant effects on liver damage as compared with Sample-B group. Therefore it seems that more researches on variation according to the increase of Injin dose must be continued for curing liver diseases.

• 대한상한금궤의학회지
• /
• 제4권1호
• /
• pp.29-36
• /
• 2012

Aim : To investigate the hepatotective effect of Gardenia jsaminodes and Glycine max aqueous extract against D-galactosamine (d-GalN, 300mg/kg body weight) was administered to the male Sprague Dawley (SD) rats. Materials and Methods : The study was carried out on male SD rats (age matched, weight $250{\pm}10g$). Experimental groups divided four: Normal group (Nor) was administered saline, Control (Con) group was administered saline after d-GalN treatment. Experimental group (Exp) was administered Gardenia jsaminodes (200 mg/kg; Ga group), Glycine max (700 mg/kg; Gl group), and Gardenia jsaminodes+Glycine max (200 mg/kg+700 mg/kg, GG group) during 14 days(n=5). Results : d-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase but decreased total cholesterol and triglyceride. Treatment with Gardenia jsaminodes extract significantly protected the liver in d-GalN administered rats. Conclusion : Gardenia jsaminodes aqueous extract and Gardenia jsaminodes+Glycine max extract possesses hepatoprotective potential, thus validating its use in alleviating toxic effects of d-GalN.

• 대한한방내과학회지
• /
• 제19권1호
• /
• pp.39-56
• /
• 1998

In order to investigate the protective effect of Samduhaejungtang on the liver injury of rats induced by $CCl_4$ and d-galactosamine, the serum transaminase(GOT& GPT), alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities and triglyceride, total bilirubin amounts for serum component were measured. All animals were divided into 4 groups, those were normal group(untreated), control group(treated with vehicle 0.9% Saline solution), sample Ⅰ group(1000mg/kg administrated), sample Ⅱ group(2000mg/kg administrated). The results were as follows : 1. The inhibitory effects of the serum GOT activities in rats induced by $CCl_4$ were noted in both sample Ⅰ group(p<0.01) and sample Ⅱ group(p<0.001). In serum GPT activities, sample Ⅱ group(p<0.05) only showed the inhibitory effects. 2. The inhibitory effects of the serum ALP activities in rats induced by $CCl_4$ were noted in both sample Ⅰ group(p<0.01) and sample Ⅱ group(p<0.001). 3. The inhibitory effects of the serum LDH activities in rats induced by $CCl_4$ were noted in sample Ⅱ group(p<0.05). 4. The increases of the serum triglyceride content level in rats induced by $CCl_4$ were inhibited in both sample Ⅰ group(p<0.01) and sample Ⅱ group(p<0.001). 5. The increases of the serum total bilirubin content level in rats induced by $CCl_4$ were inhibited in both sample Ⅰ group(p<0.01) and sample Ⅱ group(p<0.001). 6. The inhibitory effects of the serum GOT& GPT activities in rats induced by d-galactosamine were noted in sample Ⅱ group(p<0.001), but sample Ⅰ group was not recongnized. 7. The significantly inhibitory effects of in the serum LDH activities in rats induced by d-galactosamine were note in sample Ⅱ group(p<0.05). 8. The increases of the serum triglyceride content level in rats induced by d-galactosamine were inhibited in sample Ⅱ group(p<0.01). 9. The increases of the serum total bilirubin content level in rats induced by d-galactosamine were inhibited in sample Ⅱ group(p<0.05). According to the above results, it is considered that Samduhaejungtang has protective effect against liver injury in rats induced by $CCl_4$ and d-galactosamine.

• 한국자원식물학회지
• /
• 제28권5호
• /
• pp.551-560
• /
• 2015

본 연구는 산겨릅나무 세포배양 추출물이 D-galactosamine에 의해 유발된 간독성에 따른 보호 효과를 살펴보았다. 간 조직 내 국소적 지방 변성과 염증세포 침윤은 산겨릅나무 세포배양 추출물을 처리한 실험군에서 크게 감소되는 경향을 보였다. 또한 산겨릅나무 세포배양 추출물을 처리한 실험군은 간 손상에 의해 급격히 증가된 AST와 ALT, LDH 및 ALP의 활성과 조직 내 지질함량과 과산화지질함량이 감소되는 것으로 나타나 산겨릅나무 세포배양 추출물이 D-galactosamine으로 인한 혈중 효소활성과 조직 내 지질함량을 개선하는 것으로 조사되었다. 이와 더불어 산겨릅나무 세포배양 추출물을 처리한 실험군은 염증반응을 촉진시켜 조직 상해 및 괴사를 유도하는 TNF-α의 발현 수준이 간독성을 유발한 실험군에 비해 낮은 것으로 확인되었고 항산화효소의 활성을 효과적으로 조절하였다. 이러한 결과로 미루어 보아 산겨릅나무 세포배양 추출물은 D-galactosamine에 의한 조직 괴사를 감소시키고 혈중 효소의 활성과 조직 내 지질함량을 개선할 뿐만 아니라 염증 반응 인자의 발현과 항산화효소 활성을 조절하고 있어 간독성에 대한 보호효과가 매우 높은 것으로 사료된다.

• Journal of Acupuncture Research
• /
• 제25권6호
• /
• pp.95-107
• /
• 2008

Objectives : The main purpose of this research is to observe the effects of herbal acupuncture(HA; herbal acupuncture) with Chijadaehwangtang applied to the Gansu($BL_{18}$) on D-galactosamine-induced liver injury in rats. Methods : According to HA concentration, the experimental rats were divided 5 groups(control group, saline group, CP-1, CP-2, CP-3 group). In the control group, we first injected D-galactosamine and then didn't treated. In the saline group, we first injected D-galactosamine and then injected saline. In the CP-1, CP-2, CP-3 group, we first injected D-galactosamine and then injected HA with Chijadaehwangtang applied to the Gansu($BL_{18}$), each 25.3mg/kg, 12.7mg/kg, 5.1mg/kg. HA with Chijadaehwangtang was treated at $20{\mu}{\ell}$ every second day, total 10 times in 20 days. We observed the changes of $\gamma$-GTP, GOT, GPT, total bilirubin, LDH, ALP, total cholesterol, triglyceride, SOD, Catalase and hepatic tissues. Results : 1. In the changes of $\gamma$-GTP, GOT contents, as compared with control group, CP-2 group was significantly decreased. 2. In the changes of GPT content, CP-1, CP-2, CP-3 groups as compared with control group were significantly decreased. 3. In the changes of LDH content, CP-3 group as compared with control group was significantly decreased. 4. In the changes of ALP content, all groups as compared with control group were significantly decreased. 5. In the changes of SOD, Catalase contents, CP-2 group as compared with control group was significantly increased. 6. In the morphological and histopathological changes of hepatic tissues, CP-2, CP-3 groups as compared with control group were improved. Conclusions : we observed HA with Chijadaehwangtang applied to the Gansu($BL_{18}$) can be effective treatment on hematological recovery and regenerative process in morphological liver change. Further studies about their underlying mechanism and concentrations may be needed to use HA with Chijadaehwangtang for liver injury clinically.

• 대한한의학회지
• /
• 제15권2호
• /
• pp.156-172
• /
• 1994

This study was performed to evaluate the effect of the Gamichunggantang on recovering from liver injury induced by d-galactosamine and ${\alpha}$-naphthylisothiocyanate. and following results were obtained. 1. The increases of the serum GOT. GPT activities in mice induced by d-galactosamine and ${\alpha}$-naphthylisothiocyanate were inhibited significantly by the administration of the Gamichunggantang. 2. The increases of the total cholesterol. triglyceride and blood urea nitrogen levels in mice induced by d-galactosamine and ${\alpha}$-naphthylisothiocyanate were significantly inhibited by the administration of the Gamichunggantang. 3. The increase of the ALP activity in mice following liver injury induced by d-galactosamine and ${\alpha}$-naphthylisothiocyanate were inhibited to an extend. but it was not significant. 4. It is considered that the efficacy of the Gamichunggantang on liver cell injury depends on its concentration rate.

• 대한한방내과학회지
• /
• 제22권3호
• /
• pp.309-320
• /
• 2001

Objectives : This study was done to investigate the protective effects of Samgiinjin-tang on liver injury of rats induced by CCI4 and d-galactosamine. Methods: All animals were divided into .5 groups, those were normal group(untreated), control group(treated with 0.9% Saline solution), sample I group(2,250mg/kg administrated), sample II group(4,500mg/kg administrated), Silymarin 200mg/kg administrated group. Liver injury of rats were induced by CCI4 and d-galactosamine, and then the serum transaminases(ALT&AST) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities, liver weight, lipid peroxidation and catalase, glutathione S-transferase(GST) for enzyme activities were measured. Results : The inhibitory effects on the serum ALT, AST activities in liver injury of rats induced by CCI4 were noted in both sample I and sample II group. The inhibitory effects on the serum ALP, LDH activities and the Lipid peroxidation of Mitochondria & Cytosol were noted in only sample II group. The decreased effects on the GST activities of Homogenate & Cytosol were inhibited in both sample I and sample II groups. The decreased effects on the GST activities of Mitochondria & Microsome were inhibited in sample II group. The inhibitory effects of the serum ALT, AST, LDH activities in liver injury of rats induced by d-galactosamine were noted in both sample I and sample II groups. In serum AST activities, sample II group. Conclusions : Samgiinjin-tang has protective effects against liver injury of rats induced by CCI4 and d-galactosamine. So it is required to study about the actions of mutual relation of medicines and patho-mechanism by experiment.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.605-608
• /
• 2003

헛개나무 열수 추출물은 알코올 분해 및 D-galactosamine/LPS와 $CCl_4$에 대한 간 보호에 탁월한 효과를 보였다. Rat(SD)에 알코올(50%, 10 ml/kg)을 경구 투여하여 숙취를 유발한 후 헛개열매 열수 추출물을 경구 투여한 결과 혈중 알코올 농도가 크게 감소하였다. 또한 D-galactosamine($600{\mu}g/kg$)/LPS($600{\mu}g/kg$)와 $CCl_4$(1.0ml/kg)를 처리한 후 헛개열매 열수 추출물을 투여한 결과 간 기능 수치인 AST, ALT 및 LDH 값이 상당히 감소함을 알 수 있었다.

• 대한본초학회지
• /
• 제28권2호
• /
• pp.17-23
• /
• 2013

Objectives : To investigate the hepatoprotective effect of Gardeniae Fructus (Ga) and Glycine semen preparatum (Gl) aqueous extract against D-galactosamine (D-GalN, 300mg/kg body weight) was administered to the male Sprague Dawley (SD) rats. Materials and Methods : The study was carried out on male SD rats (age matched, weight $250{\pm}10$ g). Experimental groups (Exp) divided four : Normal group (Nor) was administered saline, Control (Con) group was only received D-GalN (300 mg/kg) intraperitoneally. Exp was orally administered Ga (200 mg/kg; Ga group), Gl (700 mg/kg; Gl group), and Chizadochi-Tang (200 mg/kg+700 mg/kg, GG group) after D-GalN treatment during 14 days (n=6). Results : D-GalN administration induced hepatotoxicity in rats which was manifested by increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) but decreased total cholesterol (HDL C) and triglyceride (TG). The serum TG concentrations were significantly increased ($^{\sharp}p$ <0.05) in the Ga group compared with Con. AST and ALP activities were significantly decreased ($^{\sharp}p$ <0.05) in the all experimental groups compared with Con. ALT activities were significantly decreased ($^{\sharp}p$ <0.05) in the Ga group compared with Con. LDH activities were significantly decreased ($^{\sharp}p$ <0.05) in the GG group compared with Con. On the light microscopic study, a number of vacuole were observed in the Con, but decreased in experimental groups. Conclusion : Ga aqueous extract and Chizadochi-Tang extract possesses hepatoprotective potential, thus validating its use in alleviating toxic effects of D-GalN.

• 생명과학회지
• /
• 제25권5호
• /
• pp.539-547
• /
• 2015

본 연구는 다슬기(Semisulcospira libertine) 열수 추출물이 D-galactosamine에 의해 급성 간독성이 유도된 흰쥐에 미치는 보호 효과를 조사하였다. 다슬기 열수 추출물은 D-galactosamine에 의해 유발된 간 조직 내 국소적 지방변성과 염증세포 침윤을 크게 완화하여 대조군과 유사하게 보호하는 경향을 보였다. 또한 다슬기 열수 추출물을 처리한 실험군은 간 손상 지표 효소인 AST와 ALT, LDH 및 ALP의 활성이 대조군 수준으로 유지되었으며 간조직 내 지질함량과 과산화지질함량이 감소되는 것으로 나타나 다슬기 열수 추출물이 D-galactosamine으로 인한 혈중 효소 활성과 조직 내 지질함량을 개선하는 것으로 조사되었다. 또한 다슬기 열수 추출물을 처리한 실험군은 염증반응을 촉진시켜 조직 상해 및 괴사를 유도하는 TNF-α의 발현을 억제하고 있어 염증 반응에서 세포 손상을 감소시키는 데 관여하는 것으로 나타났다. 따라서 다슬기 열수 추출물은 D-galactosamine에 의한 조직 괴사를 감소시키고 혈중 효소의 활성과 조직 내 지질함량을 개선하는 효과를 나타내고 있으며 염증 반응 인자의 발현과 항산화효소 활성을 조절하여 간독성에 대해 보호 효과가 높을 것으로 생각된다.