• 한국식품영양과학회지
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• 제31권5호
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• pp.905-909
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• 2002

일상생활에서 애용되고 있는 감자는 조리 시 껍질이 거의 폐기되고 있는 점을 감안하여, 폐기 농산물의 재활용의 측면에서 감자껍질(Solanum tubverosum Peel)을 수거하여 추출, 분획한 후 항균 및 항발암 효과를 살펴보았다. 항균성 검사로는 5가지의 균주에 대한 감자껍질의 분획물의 농도별 항균활성을 측정한 결과, 전반적으로 SPMEE에서 가장 큰 항균력을 보였으며, 특히 Pseudomonas aeruginosa 균주에서는 앞서 4가지 균에서는 활성이 없던 수층인 SPMA에서 항균활성이 크게 나타났다 감자껍질의 암세포 증식억제 효과를 MTT assay로 실험 한 결과,3종류의 암세포 HepG2, HeLa 및 MCF- 7세포주에서 모두 ethylether분획층인 SPMEE층에서 암세포 증식억제 효과가 크게 나타났으며 특히 MCF-7의 경우는 저농도 첨가 시부터 SPW층에서 높은 억제효과를 보였다. 또 HepG2세포를 이용한 감자껍질의 분획물의 암예방 QR 유도 활성효과를 본 결과, butanol 분획층인 SPMB에서 괄목할 만큼 높은 QR 유도 활성이 증가되었다. 본 실험 결과, 감자껍질에는 전반적으로 SPMEE층에서 높은 항균성을 보여 항균제로서의 개발가능성이 충분히 엿보이며, 나아가 암세포 증식억제효과도 SPMEE층에서 아주 컸다. SPMB와 SPMEA층에서도 그 효과가 돋보인다. 암을 예방하는 inducer는 SPMB층에 많이 존재하는 것으로 사료되며 이와 같은 연구결과를 기초로 하여 폐기 감자껍질을 이용한 단계적인 생리활성 물질의 분리 동정이 계속 이루어져 폐기식품 활용 및 건강 대체 식품 개발의 연구 대상으로서 기대가 되는 바이다.

• Advances in nano research
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• 제11권6호
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• pp.657-665
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• 2021

The present work aimed to explore green approach via aqueous leaves extract of Murraya koenigii (ALEMk) for the synthesis of silver nanoparticles (AgNPsMk) in single step. The synthesis process was visualized with a color change and monitored by employing UV/Visible spectroscopy and a clear peak attained at 420 nm confirming the synthesis of AgNPsMk. The possible functional groups present in the extract which participated in the synthesis of AgNPsMk were identified with the help of FTIR spectroscopy. Further characterization using TEM images revealed the spherical shape of AgNPsMk with average particle size of 20 nm displaying well dispersion throughout the solution. Pronounced antioxidant activities of AgNPsMk at increased concentrations observed which evidencing strong radical scavenging ability. Moreover, AgNPsMk exhibited strong antibacterial behavior when tested against bacterial strains of Escherichia coli and Bacillus subtilis. Moving ahead, in vitro cytotoxicity work revealed potent cell viability loss appearing in AU565 and HeLa cancer cell lines on exposure to AgNPsMk at increased concentration. Finally, in vivo assessment carried out inside male Wistar rats indicated non toxic effect on examined liver tissues besides biochemical analysis including bilirubin, alkaline phosphtase (ALP) and serum glutamate pyruvate transaminase (SGPT) which found within the normal range when compared with control. The prior research work profoundly apprises the potential of green synthesized AgNPsMk to play a significant role in biomedical applications and formulations.

• 한국식품저장유통학회지
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• 제15권2호
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• pp.274-279
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• 2008

해양심층수 간장 및 일반간장을 제조하여 항돌연변이원성과 세포독성을 측정하였으며 sarcoma-180 cell을 이용하여 in vivo에서 항암효과를 살펴보았다. S. typhimurium TA98과TA100 균주를 이용한 실험에서 모든 시료에서 돌연변이원성이 없었으며, 항돌연변이원성 실험에서는 직접변이원인 MNNG($0.4{\mu}g$/plate)의 경우 TA100 균주에서 해양심층수간장의 시료농도 $200{\mu}g$/plate에서 90.9%의 높은 억제효과를 나타내었으며 4NQO($0.15{\mu}g$/plate)에 대해서는 같은 시료농도에서 62.0%의 억제효과를 나타내었다. 그리고 4NQO의 경우 TA98 균주에 대해서 해양심층수 간장은 61.7%의 억제효과를 나타내었으며 모든 시료는 농도 의존적으로 억제하는 것으로 나타났다. 세포독성 효과를 알아보기 위하여 HeLa, Hep3B, AGS, A549와 MCF-7을 사용하였다. 각 시료 추출물의 암세포 성장효과를 조사한 결과, 해양심층수 간장이 1 mg/mL의 농도에서 각각 69.4%, 70.5%, 55.6%, 82.1% 및 73.2%의 억제율을 나타내었다. 그리고 해양심층수 간장은 고형암 성장 억제 실험에서 대조군에 비해서 40.9%의 고형암 성장 억제효과를 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6991-6995
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• 2013

Antioxidant activity, total phenolic, total flavonoid compounds and cytotoxicity to cancer cell lines of propolis extracts from two extraction methods were investigated in this study. Propolis was collected from Phayao province and extracted with 70% ethanol using maceration and sonication techniques. The antioxidant activity was evaluated by DPPH assay. Total phenolic and flavonoid compounds were also determined. Moreover, the cytotoxicity of propolis was evaluated using MTT assay. The percentage propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless, antioxidant and flavonoid compounds of the sonication propolis extract were significant greater than using maceration. Propolis extract from sonication showed antioxidant activity by $3.30{\pm}0.15$ mg gallic acid equivalents/g extract. Total phenolic compound was $18.3{\pm}3.30$ mg gallic acid equivalents/g extract and flavonoid compound was $20.49{\pm}0.62$ mg quercetin/g extract. Additionally, propolis extracts from two extraction methods demonstrated the inhibitory effect on proliferation of A549 and HeLa cancer cell lines at 24, 48 and 72 hours in a dose-dependent manner. These results are of interest for the selection of the most appropriate method for preparation of propolis extracts as potential antioxidant and anticancer agents.

• Toxicological Research
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• 제22권4호
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• pp.391-395
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• 2006

Flavonoids are polyphenolic compounds that are ubiquitous in plants. They have been shown to possess a variety of biological activities, such as antioxidant and anticancer. Reactive oxygen species (ROS) lead to damages of cellular molecules and it is the one of various factors to induce cancer. The one of flavonoids, Luteolin, was found to scavenge 1,1-diphenyl-2-piculhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). Moreover luteolin showed protection on hamster lung fibroblast cells (V79-4 cell) damage induced by $H_2O_2$. And then it was investigated whether it may show cytotoxicity effect against various cancer cells by MTT, Luteolin at $10{\mu}g/ml$ showed the cell viability of 63.2%, 34.7%, 18.4% and 71.4% against NCl-H460, HeLa, U937 and MCF-7, respectively. As a result, luteolin shows more sensitive to U937 cells among the tested cancer cell lines. In summary, luteolin has antioxidant and cytotoxicity effect or various cancer cell lines.

• BMB Reports
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• 제32권4호
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• pp.379-384
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• 1999

Taxol, a natural product with significant anti-tumor activity, stabilizes microtubules and arrests cells in the G2/M phase of the cell cycle. It has been reported that taxol has additional effects on the cell such as an increase in tyrosine phosphorylation of proteins and activation of mitogen-activated protein (MAP) kinase. This phosphorylated kinase translocates into the nucleus and phosphorylates its substrate c-jun, c-fos, ATF2, and ATF3. The MAP kinase family is comprised of key regulatory proteins that control the cellular response to both proliferation and stress signals. First examination was cytotoxicity and apoptosis-induced concentration with paclitaxel in HeLa cell. A half-maximal inhibition of cell proliferation ($IC_{50}$) occurred at 13 nM paclitaxel. When DNA fragmentation was analyzed by agarose gel electrophoresis, a nucleosomal ladder became evident 24 h after a taxol (50 nM) addition to the cells. In addition, an apoptotic body was detected by electron microscopy. Taxol-treated cells were arrested at the S phase at 10 nM. Treatment of 50 nM taxol activated the extracellular signal-regulated protein kinase (ERK1), and a fraction of the activated MAP kinases entered the nucleus. It was also discovered that nucleus substrates c-jun was phosphorylated and activated in the cell. The activated ERK1 could subsequently translocate into the nucleus and phosphorylate its substrate c-jun as well. This study suggests that taxol-induced apoptosis might be related with signal transduction via MAP kinases.

• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.4915-4918
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• 2014

Background and Aims: Advances in the treatment of cervical cancer over the last decade have predominantly involved the development of genes directed at molecular targets. Gene therapy is recognized to be a novel method for the treatment of cervical cancer. Genes can be administered into target cells via nanocarriers. This study aimed to develop systemically administrable nano-vectors. Floate (Fa) containing gene loaded nanoparticles (NPs) could target HeLa human cervical cancer cells through combination with receptors on the cells to increase the nuclear uptake of genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly (D, L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro transfection efficiency of Fa-NPs/DNA was evaluated in HeLa cells and human umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183 nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher than naked DNA in HeLa cells. However, in HUVECs, no significant difference appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function as excellent materials for gene loading. This nano-approach could be used as tumor cell targeted medicine for the treatment of cervical cancer.

• Mycobiology
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• 제46권4호
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• pp.448-451
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• 2018

Fungal perylenequinones have photodynamic activity and are promising photosensitizers for photodynamic therapy (PDT). Here, we investigated the bactericidal and antitumor activities of phleichrome from the fungal perylenequinone family in vitro. Photodynamic bactericidal activity of phleichrome was analyzed by agar-well diffusion method under dark and illuminated conditions. The photodynamic antitumor activity of phleichrome was analyzed in MCF-7, HeLa, SW480, and HepG2 human cancer cell lines using in vitro cytotoxicity assays. Photodynamic bactericidal activities against Gram-negative and Gram-positive bacteria were species-specific. Antitumor activity against all tumor cell lines increased under the illuminated condition. Depending on the results of the analyses, Phleichrome has potential for further drug development related to its antibacterial and antitumor activities.

• 한국식품과학회지
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• 제32권6호
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• pp.1371-1378
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• 2000

S. typhimurium TA98과 TA100을 이용한 Ames test에서는 아가리쿠스버섯 메탄올 추출물 모두 시료자체의 돌연변이원성은 없는 것으로 나타났다. 아가리쿠스버섯 메탄올 추출물$(200\;{\mu}g/plate)$ 중 TA98 균주를 이용한 항돌연변이 효과를 확인한 결과 직접변이원인 4NQO에 대해 92.4% 그리고 간접변이원인 Trp-P-1과 $B({\alpha})P$에 대해 각각 81.9%와 83.4%의 높은 억제효과를 나타내었다. 또한 TA100 균주에서 4NQO는 94.7%의 가장 높은 억제효과를 나타내었다. MNNG, Trp-P-1 및 $B({\alpha})P$은 각각 87.3%, 89.9% 그리고 92.3%의 억제율을 보였다. 각각의 변이원 물질에 대한 아가리쿠스버섯 분획물 $(200\;{\mu}g/plate)$의 항돌연변이 효과에서는 MNNG와 $B({\alpha})P$에 대해서는 분획물 모두가 80% 이상의 높은 억제율을 보였고, 4NQO는 TA98, TA100 두 균주 모두 에틸 아세테이트 분획물에서 95% 이상의 가장 높은 억제율을 나타내었다. 그리고 Trp-P-1에서는 TA98 균주에서 물층을 제외한 분획물이 높은 억제율을 나타내었고, TA100 균주에 대해서도 분획물 모두가 80% 이상의 높은 억제율을 나타내었다. 각종 암세포에 대한 아가리쿠스버섯 메탄올 추출물의 저해효과는 시료 1 mg/mL 투여시 MCF7 82.9%, A549 86.5%, HT1080 65.5%, Hep3B 84.3%, HeLa 91.9%, KAROIII 88.7% 그리고 K562세포에서는 82.0%의 억제효과를 나타내었다. 인간 정상 간세포 WRL68에 대한 시료 농도에 따른 세포독성효과는 1 mg/mL의 시료를 첨가시 50% 이하의 생육억제율을 나타냄으로써 정상세포에 대해서는 낮은 독성효과를 나타낸다는 사실을 알 수 있었다. 아가리쿠스버섯 분획물들에 대한 억제효과에서는 유방암 세포인 MCF7이 시료를 1 mg/mL 첨가하였을때 물층과 부탄올층을 제외한 분획물에서 90% 이상의 높은 억제효과를 보였다. 또한 에틸 아세테이트 분획물이 다른 분획물에 비해 각각의 암세포에 대해서도 1 mg/mL 첨가시 80% 이상의 높은 억제효과를 보였다. 그러나 물 분획물은 암세포에 대해 상대적으로 낮은 억제효과를 나타내었다.

• 대한한의학방제학회지
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• 제7권1호
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• pp.107-120
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• 1999

The purpose of this Study was to investigate effects of KaegiBokryengHwan(KBH) on anti-tumor, immunocytes and nitric oxide(NO). This Study estimated the proliferation of L1210 cell lines, HeLa cell lines, SK-OV3 cell lines, MCF-7 cell lines, balb/c mouse 3T3 cell lines, mouse thymocytes and mouse splenocytes and NO production from peritoneal macrophages in vitro. and estimated the proliferation of L1210 cells, mouse thymocytes and splenocytes and NO production from peritoneal macrophages and body weight in L1210 cells-transplanted mice in vivo. The result were obtained as follow ; 1. KBH inhibited significantly SK-OV3 cell lines in vitro. 2. KBH was accelerate significantly the proliferation of balb/c mouse thymocytes in vitro. 3. KBH increased significantly NO production from peritoneal macrophages in vitro. 4. KBH didn't effect the cytotoxicity of L1210 cells in L1210 cells-transplanted mice. 5. KBH was accelerate the proliferation of splenocytes in L1210 cells-transplanted mice. 6. KBH increased NO production from peritoneal macrophages in L1210 cells-transplanted mice. 7. KBH increased the body weight as comparing with control group in L1210 cells-transplanted mice.