• 한국식품위생안전성학회지
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• 제38권6호
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• pp.464-475
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• 2023

본 연구에서는 온라인 마켓에서 판매되는 50개 복어제품의 종판별 및 표시사항 일치여부 모니터링을 수행했다. 복어의 종판별을 위해 cytochrome c oxidase subunit I 및 cytochrome b 유전자의 염기서열을 분석하여 NCBI GenBank 데이터베이스에 등록되어있는 생물종의 염기서열과 비교 후 계통 분석을 수행했다. 참복, 흰점참복, 까치복, 복섬, 검복, 국매리복, 흑밀복 총 7종이 동정되었으며, 35개 제품(70%)에서 표시사항과의 불일치를 나타냈다. 12개의 제품(24%)에서는 식품공전에서 제시한 식용 가능한 복어 21종의 국명 대신 일반명(복어)을 사용하였다. 가공 정도별 불일치율은 다중가공 제품(n=9, 81.8%)이 단순가공 제품(n=26, 66.7%)보다 높은 비율을 보였으며, 원산지별 불일치율의 경우 중국산 제품(n=8, 80%)이 국산 제품(n=26, 66.7%)보다 높은 비율을 보였다. 시장명, 방언 등의 이름이 혼용되어 다수의 복어종을 밀복, 졸복으로 표시하였다. 이러한 분류체계의 어려움으로 인해 흰점참복, 국매리복과 같은 식용불가 복어가 식용 가능한 복어인 졸복으로 혼용되어 판매되는 것이 확인되었다. 따라서 식용 가능한 복어를 정확히 분류할 수 있는 방법의 개발이 필요하며, 수입 및 국내 유통 복어 제품의 주기적인 모니터링이 필요하다.

• 한국패류학회지
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• 제26권4호
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• pp.285-290
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• 2010

본 연구는 우리나라 무안, 태안, 여수, 제주 지역 및 중국 영성, 대련 지역의 낙지 개체군의 종 및 집단분석을 위해 미토콘드리아 CO1유전자의 염기서열을 조사하였다. 유전자 분석은 총 60 개체로부터 6개의 haplotype이 조사되었다. 제주 개체군인 경우 모든 개체 (N = 10) 가 다른 집단에서는 관찰되지 않은 A haplotype으로 구성되어 있어 다른 지역과 뚜렷한 차이를 나타내었다. 이들 haplotype은 MEGA 4 분석에 의해 두 개의 clade로 나뉘어지는데 하나는 무안, 태안, 여수, 중국 대련집단이었고 다른 하나는 제주, 중국 영성집단으로 나뉘는 구조를 하고 있었다. 집단 간 유연관계 분석에서도 같은 현상이 관찰되었다. Group A에 속하는 무안, 태안, 여수, 대련 집단의 낙지 개체군은 group B (제주, 영성) 에 속하는 집단 개체군 보다 유전적 거리가 아주 가까웠다. 본 연구에서 사용한 CO1 universal primer는 종 판별 유전자 마커로서 낙지류에서도 유용하게 활용될 수 있었으나 partial CO1 유전자 내의 변이가 많지 않기 때문에 집단분석에는 한계가 있으나, 지역적 거리 및 장벽 그리고 서식지 차이 등에서 오는 제한적 gene flow에 대한 논의는 가능할 것으로 판단된다.

• Animal Systematics, Evolution and Diversity
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• 제36권1호
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• pp.10-14
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• 2020

Pagurus is the most diverse hermit crab genus in Korea. In this study, the cytochrome c oxidase subunit I (COI) and 16S rRNA of 24 individuals from four Korean Pagurus species (i.e., 7 Pagurus brachiomastus, 8 P. proximus, 8 P. simulans, and 1 P. rectidactylus) were sequenced and analyzed. No genetic difference was found between the COI and 16S rRNA sequences of P. brachiomastus and P. simulans, and the COI sequences of P. rectidactylus and P. quinquelineatus (comparative species from NCBI). Considering the morphological and ecological characteristics together, we assume that P. simulans and P. rectidactylus are subspecies of P. brachiomastus and P. quinquelineatus, respectively. This study should facilitate further research on the taxonomic status of these species.

• Animal Systematics, Evolution and Diversity
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• 제35권4호
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• pp.182-185
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• 2019

In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.

• 한국식품위생안전성학회지
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• 제36권4호
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• pp.331-341
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• 2021

본 연구에서는 DNA barcode 시험법을 이용하여 시중 유통 중인 도미와 옥돔 수산가공식품의 위변조현황을 분석하였다. 참돔 12건, 돌돔 4건, 황돔 7건, 감성돔 2건, 나일틸라피아 7건, 옥돔 6건, 옥두어 8건 총 46건의 시료에 대해 실험을 진행하였다. 생물 종 판별에 주로 이용되는 mitochondrial DNA의 COX I (cytochrome C oxidase subunit I) 유전자 영역을 분석하여 원재료의 종을 판정하였다. NCBI에서 제공하는 BLAST Search 프로그램을 이용하여 분석된 염기서열과 NCBI에 등록된 각각 어류의 유전자 염기서열을 비교하였다. 분석 결과 염기서열 상동성(identity)이 97% 이상인 종을 원재료 종으로 판별하였다. DNA barcode 시험법을 이용해 시중 유통되는 참돔, 돌돔, 황돔, 감성돔, 나일틸라피아, 옥돔, 옥두 수산가공품 46건에 대해 조사한 결과 위변조 사례는 나타나지 않았다. 그러나 시장에서 통용되는 일반명칭과 식품공전에 기술된 표준명칭이 상이하여 소비자의 혼란을 야기할 수 있어 수산물 가공식품 표기에 일반명칭과 더불어 표준명칭 또는 학명을 같이 기술하여야 할 것으로 판단되었다.

• ALGAE
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• 제37권4호
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• pp.249-264
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• 2022

Two genera of the Rhodymeniales, Halopeltis and Leptofauchea, are here reported for the first time from the Hawaiian Islands and represent the deepest records for both genera. Molecular phylogenetic analyses of cytochrome oxidase subunit I (COI), rbcL, and large subunit ribosomal DNA (LSU) sequences for Hawaiian specimens of Leptofauchea revealed one well-supported clade of Hawaiian specimens and three additional lineages. One of these clades is described here as Leptofauchea huawelau sp. nov., and is thus far known only from mesophotic depths at Penguin Bank in the Main Hawaiian Islands. L. huawelau sp. nov. is up to 21 cm, and is the largest known species. An additional lineage identified in the LSU and rbcL analyses corresponds to the recently described L. lucida from Western Australia, and is a new record for Hawai'i. Hawaiian Halopeltis formed a well-supported clade along with H. adnata from Korea, the recently described H. tanakae from mesophotic depths in Japan, and H. willisii from North Carolina, and is here described as Halopeltis nuahilihilia sp. nov. H. nuahilihilia sp. nov. has a distinctive morphology of narrow vegetative axes that harbor constrictions along their length. The current distribution of H. nuahilihilia includes mesophotic depths around W. Maui, W. Moloka'i, and the island of Hawai'i in the Main Hawaiian Islands. Few reproductive characters were observed because of the small number of specimens available; however, both species are distinct based on phylogeny and morphology. These descriptions further emphasize the Hawaiian mesophotic zone as a location harboring many undescribed species of marine macroalgae.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.683-688
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• 2015

Human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus Diphyllobothrium. In mainland China, so far 20 human cases of Diphyllobothrium infections have been reported, and the etiologic species were identified as D. latum and D. nihonkaiense based on morphological characteristics or molecular analysis. In the present study, proglottids of diphyllobothriid tapeworms from 3 human cases that occurred in Heilongjiang Province, China were identified as D. nihonkaiense by sequencing mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 5 (nad5) genes. Two different cox1 gene sequences were obtained. One sequence showed 100% homology with those from humans in Japan. The remaining cox1 gene sequence and 2 different nad5 gene sequences obtained were not described previously, and might reflect endemic genetic characterizations. D. nihonkaiense might also be a major causative species of human diphyllobothriasis in China. Meanwhile, the finding of the first pediatric case of D. nihonkaiense infection in China suggests that infants infected with D. nihonkaiense should not be ignored.

• ALGAE
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• 제35권2호
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• pp.157-165
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• 2020

Culture isolates of the genus Hypoglossum (Delesseriaceae, Rhodophyta) were obtained and their development and morphological structure over many years were followed in the laboratory. Molecular data (rbcL, large subunit ribosomal DNA, and cytochrome c oxidase subunit I) were obtained from these strains and evidence presented to recognize the new species: Hypoglossum sabahense from Sabah, Malaysia. Because various aspects of morphology in culture specimens differ significantly from types based on field specimens we have to rely mainly on the molecular criteria in ascribing a new taxonomic name here. This also is complicated by the major lack of molecular phylogenetic evidence for Hypoglossum and other Delesseriaceae. The 'Germling Emergence Method' and 'serendipity' are proving valuable in discovering significant new taxa from laboratory cultures which otherwise might never be known.

• The Plant Pathology Journal
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• 제35권1호
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• pp.51-62
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• 2019

A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권4호
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• pp.221-226
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• 2022

A filarial nematode was found in a blood sample of an Anas falcata individual collected in South Korea in 2018. Phylogenetic analysis based on partial cytochrome C oxidase subunit I (COI) sequences placed the nematode as a novel genus of the family Onchocercidae and as closely related to Mansonella species, Chandlerella quiscali, and filarial nematodes recently reported in avian species. However, different phylogenetic relationship was observed in the NADH dehydrogenase subunit 5 and 12S rRNA-based phylogenetic trees, which might indicate the filarial nematode found in this study was not defined to belong to the known specific genera of the family Onchocercidae. The screening of 105 additional avian blood samples retrieved only one 12S rRNA-targeting polymerase chain reaction (PCR)-positive sample, which indicates that filarial nematode infection is rare in wild birds or that it occurs below the detection limit of PCR in blood samples. Nevertheless, considering the recent findings about ancient interactions between birds and human pathogenic filarial nematodes and their pathogenic potential in several avian species, additional exploration of novel filarial nematodes in wild birds remains necessary.