• Title/Summary/Keyword: cut-off temperature

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Purification and Characterization Sucrose phosohorylase in Leuconostoc mesenteroides NRRL B-1149 (Leuconostoc mesenteroides NRRL B-1149의 Sucrose phosohorylase의 분리와 특성 연구)

  • Lee Jin Ha;Park Jun Seong;Park Hyen Joung;Cho Jae Young;Choi Jeong Sik;Kim Do Man
    • KSBB Journal
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    • v.19 no.5
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    • pp.363-367
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    • 2004
  • Leuconostoc mesenteroides NRRL B-1149 produces various glucoseyltransferases for the synthesis of dextran, levan and glucose-1-phosphate using sucrose as a substrate. A sucrose phosphorylase (1149SPase) was purified from L. mesenteroides NRRL B-1149 culture by using hollow fiber filtration (30 kDa cut off), Toyopearl DEAE 650 M column chromatography and following two times of DEAE-Sepharose column chromatographies. The specific activity of the purified 1149SPase was 25.7 (U/mg) with $16\%$ yield. The 1149SPase showed a molecular size of 56 kDa on denatured $10\%$ SDS-PAGE. The N-terminal amino acid sequence of the enzyme was MEIQNKAM. The optimum pH and temperature of this enzyme were 6.2~6.5 and 37^{circ}C, respectively. It had an apparent K_{m} of 6.0 mM and K_{cat} of 1.62/s for sucrose. 1149SPase crystal was formed by hanging drop diffusion technique using 20 mM calcium chloride dihydrate, 100 mM sodium acetate trihydrate pH 4.6 and $30\%$ 2-methyl-2,4-pentanediol as vaporizing and reservation solution. The 1149SPase catalyzes transferring of glucose from isomaltose or sucrose to salicin and salicyl alcohol by disproportionation reaction or acceptor reaction and synthesized two acceptor products, respectively.

Lake Water Treatment Using a Ultrafiltration Membrane Process of Hollow Fiber Type (중공사형 한외여과 막분리 공정에 의한 하천수 처리)

  • 박진용
    • Membrane Journal
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    • v.7 no.1
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    • pp.39-47
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    • 1997
  • The self-designed membrane system was tested to examine the performance of the hollow fiber type polysulfone ultrafiltration(UF) membrane for the treatment of pure water(the 3rd treated water). The molecular weight cut-off's (MWCO) of the membranes used in this study were 5, 000 and 10, 000, respectively. The recovery rate, the ratio of permeate flow rate to the feed flow rate, increased as the temperature rose. The values of MWCO obtained in this study, using 2, 000 ppm polyethylene glycol and dextran solutions with various molecular weight, showed higher values than those suggested by SKI. Based on the results of the primary experiments, the water of the Gongji-stream, in which water quality is deteriorated by the inflow of domestic wastewater, was selected for the UF membrane test. Biological oxygen demand(BOD), total solids, and turbidity of the treated water had much lower values than those of the source water. Therefore, this study confirmed the possibility of the domestic water treatment using the hollow fiber type UF membrane.

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Curl Wave Formation and Hair Damage by the Rinsing (중간 세척이 컬 웨이브 형성과 모발 손상에 미치는 영향)

  • Moon, Soon-Joo;Kim, Min-Joung
    • Journal of the Korean Society of Fashion and Beauty
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    • v.4 no.3 s.9
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    • pp.13-17
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    • 2006
  • Perm is one the of the most common thing in a beauty salon like hair-cut and hair perm needs processing solutions which is chemical substances. Those chemical substances damage the hair cuticle. Damaged hair cuticles with mistakes of perm will follow with the next performance and it will cause more damage to hair. As damage of hair cuticle become worse, it is harder to recover the hair cuticle like before. Therefore, this study shows that plain rinse is the way to reduce the damage of hair cuticle with perm as much as possible. With experiments, I suggest the right way to do plain rinse to satisfy customers' demand and reduce the damage. During the research, I figured that plain rinse with the knowledge, structure of hair cuticle, principle of perm and plain rinse, is very needed. The hair with plain rinse have more elastic curl and have less scales which came off from hair cuticle than the hair without plain rinse. This experiment concludes that hairdresser has to figure out about the time, temperature and type of water depends on the degree of damaged hair of customers. In order to perm, there is no way to not to use chemical substances to perm. Therefore, hairdresser has to choose suitable processing solutions and right steps, and as this is the age of 'well-being' boom, hairdresser must try to keep the healthy hair. Consequently, in order to meet the demands and conditions of customers, hairdresser needs to find the right method to do plain rinse and use the method in perm.

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Electrical and Physical Properties of Sheath-core Type Conductive Textile Sensor with Home-Textile (Sheath-core 구조 전도사 섬유센서의 Home-Textile 적용을 위한 전기·물리학적 특성연구)

  • Cho, Kwang-Nyun;Jung, Hyun-Mi
    • Fashion & Textile Research Journal
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    • v.16 no.1
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    • pp.145-152
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    • 2014
  • The usage of textile-based sensors has increased due to their many advantages (compared to IT sensors) when applied to body assessment and comfort. Textile-based sensors have different detecting factors such as pressure, voltage, current and capacitance to investigate the characteristics. In this study, textile-based sensor fabrics with sheath-core type conductive yarns were produced and the relationship between capacitance changes and applied load was investigated. The physical and electric properties of textile-based sensor fabrics were also investigated under various laminating conditions. A textile based pressure sensor that uses a sheath-core conductive yarn to ensure the stability of the pressure sensor in the textile-based sensor (the physical structure of the reaction characteristic of the capacitance) is important for the stability of the initial value of the initial capacitance value outside the characteristic of the textile structural environment. In addition, a textile based sensor is displaced relative to the initial value of the capacitance change according to pressure changes in the capacitance value of the sensor due to the fineness of the high risk of noise generation. Changing the physical structure of the fabric through the sensor characteristic of the pressure sensor via the noise generating element of laminating (temperature, humidity, and static electricity) to cut off the voltage output element to improve the data reliability could be secured.

Development of Bypass Unit for Ship Area Network Based on Legacy-line Communication (무배선 통신을 위한 선박 네트워크용 바이패스 장치 개발)

  • Jun, Ho-Ik;Kim, Hyun-Sik;Jung, Kyun Sik;Sohn, Kyung-Rak
    • Journal of Advanced Marine Engineering and Technology
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    • v.39 no.3
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    • pp.292-297
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    • 2015
  • In this paper, we reported the bypass unit for ship area networks in order to detour the communication failure regions and poor communication links. The device was composed of three parts of circuits for power cut-off, protection, and coupling transformer. Since the coupling transformer exerts a dominant influence on the performance of the by-pass unit, we have tried to find the optimal magnetic core materials and its dimensions. The prototype was passed through the performance test of insertion loss, temperature, and vibration characteristics. The insertion loss was around -2 dB in the range of 90 kHz ~ 30 MHz and the average communication speed was 59.2 Mbps in the laboratory. A pilot communication test using the developed tool was conducted in the training ship of the Korea Maritime and Ocean University. As a results of experiment, we showed that the wired communication among the heterogeneous-links in the ship area networks are possible by the bypass unit and also a high speed communication services are available in ~ Mbps by using a power-line.

Quantitative estimation of the energy ux during an explosive chromospheric evaporation in a white light are kernel observed by Hinode, IRIS, SDO, and RHESSI

  • Lee, Kyoung-Sun;Imada, Shinsuke;Watanabe, Kyoko;Bamba, Yumi;Brooks, David H.
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.67.3-68
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    • 2016
  • An X1.6 flare occurred in AR 12192 on 2014 October 22 around 14:06 UT and was observed by Hinode, IRIS, SDO and RHESSI. We analyze a bright kernel which produces a white light flare (WLF) with continuum enhancement and a hard X-ray (HXR) peak. Taking advantage of the spectroscopic observations of IRIS and EIS, we measure the temporal variation of the plasma properties in the bright kernel in the chromosphere and corona. We found that explosive evaporation was observed when the WLF occurred, even though the intensity enhancement in hotter lines is quite weak. The temporal correlation of the WLF, HXR peak, and evaporation flows indicates that the WLF was produced by accelerated electrons. To understand the white light emission processes, we calculated the deposited energy flux from the non-thermal electrons observed by RHESSI and compared it to the dissipated energy estimated from the chromospheric lines (Mg II triplet) observed by IRIS. The deposited energy flux from the non-thermal electrons is about $3.1{\times}10^{10}erg\;cm^{-2}s^{-1}$ when we assume a cut-off energy of 20 keV. The estimated energy flux from the temperature changes in the chromosphere measured from the Mg II subordinate line is about $4.6-6.7{\times}10^9erg\;cm^{-2}s^{-1}$, 15 - 22 % of the deposited energy. By comparison of these estimated energy fluxes we conclude that the continuum enhancement was directly produced by the non-thermal electrons.

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Growth of AlN/GaN HEMT structure Using Indium-surfactant

  • Kim, Jeong-Gil;Won, Chul-Ho;Kim, Do-Kywn;Jo, Young-Woo;Lee, Jun-Hyeok;Kim, Yong-Tae;Cristoloveanu, Sorin;Lee, Jung-Hee
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.15 no.5
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    • pp.490-496
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    • 2015
  • We have grown AlN/GaN heterostructure which is a promising candidate for mm-wave applications. For the growth of the high quality very thin AlN barrier, indium was introduced as a surfactant at the growth temperature varied from 750 to $1070^{\circ}C$, which results in improving electrical properties of two-dimensional electron gas (2DEG). The heterostructure with barrier thickness of 7 nm grown at of $800^{\circ}C$ exhibited best Hall measurement results; such as sheet resistance of $215{\Omega}/{\Box}$electron mobility of $1430cm^2/V{\cdot}s$, and two-dimensional electron gas (2DEG) density of $2.04{\times}10^{13}/cm^2$. The high electron mobility transistor (HEMT) was fabricated on the grown heterostructure. The device with gate length of $0.2{\mu}m$ exhibited excellent DC and RF performances; such as maximum drain current of 937 mA/mm, maximum transconductance of 269 mS/mm, current gain cut-off frequency of 40 GHz, and maximum oscillation frequency of 80 GHz.

Comparison of the effect of hand instruments, an ultrasonic scaler, and an erbium-doped yttrium aluminium garnet laser on root surface roughness of teeth with periodontitis: a profilometer study

  • Amid, Reza;Kadkhodazadeh, Mahdi;Fekrazad, Reza;Hajizadeh, Farzin;Ghafoori, Arash
    • Journal of Periodontal and Implant Science
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    • v.43 no.2
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    • pp.101-105
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    • 2013
  • Purpose: The present study aimed to measure root surface roughness in teeth with periodontitis by a profilometer following root planning with ultrasonic and hand instruments with and without erbium-doped yttrium aluminium garnet (Er:YAG) laser irradiation. Methods: Sixty single-rooted maxillary and mandibular teeth, extracted because of periodontal disease, were collected. The crowns and apices of the roots were cut off using a diamond bur and water coolant. The specimens were mounted in an acrylic resin block such that a plain root surface was accessible. After primary evaluation and setting a baseline, the samples were divided into 4 groups. In group 1, the samples were root planned using a manual curette. The group 2 samples were prepared with an ultrasonic scaler. In group 3, after scaling with hand instrumentation, the roots were treated with a Smart 1240D plus Er:YAG laser and in group 4, the roots were prepared with ultrasonic scaler and subsequently treated with an Er:YAG laser. Root surface roughness was then measured by a profilometer (MahrSurf M300+RD18C system) under controlled laboratory conditions at a temperature of $25^{\circ}C$ and 41% humidity. The data were analyzed statistically using analysis of variance and a t-test (P<0.05). Results: Significant differences were detected in terms of surface roughness and surface distortion before and after treatment. The average reduction of the surface roughness after treatment in groups 1, 2, 3, and 4 was 1.89, 1.88, 1.40, and 1.52, respectively. These findings revealed no significant differences among the four groups. Conclusions: An Er:YAG laser as an adjunct to traditional scaling and root planning reduces root surface roughness. However, the surface ultrastructure is more irregular than when using conventional methods.

Effects of $GeO_2$ Addition on the Stabilities of $PbO-Bi_2O_3-Ga_2O_3$ Glasses ($GeO_2$의 첨가가 $PbO-Bi_2O_3-Ga_2O_3$ 유리의 안정화에 미치는 영향)

  • Choi, Yong-Gyu;Heo, Jong;Ryou, Sun-Youn
    • Journal of the Korean Ceramic Society
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    • v.32 no.11
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    • pp.1269-1275
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    • 1995
  • Effects of GeO2 addition on the thermal and structural stabilities of PbO-Bi2O3-Ga2O3 glasses were studied. Thermal stabilities, as assessed by the weighted thermal stability factors [(Tx-Tg)/Tg], increased with GeO2 concentraton from 0.097 to 0.210 with the addition of 20 mol% GeO2. Increasing GeO2 content resulted in the decrease of apparent density, molar volume, refractive index and thermal expansion. On the other hand, IR transmission cut-off (λT=50%) moved from 6.73${\mu}{\textrm}{m}$ for the ternary PbO-Bi2O3-Ga2O3 glass to shorter wavelength side, 5.98${\mu}{\textrm}{m}$ for a glass containing 20mol% GeO2. There were little change with GeO2 content, however, in the activation energies for the viscous flow of approximately 140 kcal/mole within the temperature interval of 300~50$0^{\circ}C$. Addition of GeO2 to PbO-Bi2O3-Ga2O3 glasses enhanced the thermal and structural stabilities significantly at the expense of their infrared transmittance. An appropriate compsomise between these two opposite trends should be made following the specifications of the final applications.

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Volatility of Herbicides Sprayed in Zoysia japonica Turf and Bare Soil (잔디밭과 나지에 산포된 주요 잔디밭용 제초제의 휘산)

  • 김석정;박진희;죽내안지;김길웅;신동현;허영조
    • Asian Journal of Turfgrass Science
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    • v.10 no.3
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    • pp.263-270
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    • 1996
  • This experiment was conducted to investigate the loss of various herbicides by means of vola-tility from the turfgrass field and the hare ground with the different soil moisture contents and temperatures. Different herbicides were applied at the rates of 375 g a.i. /l0a of pendimethalin,250 g a.i. /l0a of napropamide, and 96.4 g a.i. /l0a of dicamba with 200 \ulcorner/10a of spray volume in the turfgrass(Zoysia japonica cut off 5cm) grown in pots(265.8 $cm^2$) and bared soil. The pots were placed in the growth chamber with 10,000 lux of light intensity(12h per day) at 25 and 35˚C for 7days. Amberlite XAD polymeric resin(20/50 mesh) was used as sampling media for herbicide airborne residues. Air flow was maintained at 10 \ulcorner /min by vacuum pump regulated with a factory calibrated flow meter. Herbicide airborne residues were extracted from the XAD resin with 300 ml of 1:1 acetone and hexane. The extracts were concentrated by rotary evaporation at 35˚C and dissolved in 1 ml MeCN for HPLC analysis. The airborne losses of the herbicide applied in the turfgrass and bare soil increased as the temperature and soil moisture contents were increased, regardless of the kinds of herbicide. Higher airborne residues was observed in the turfgrass pots than the bare soil pots. Pendimethalin and dicamba with higher vapor pressure gave rise to the increased loss of airborne herbicides, showing that 6.26 and 6.4% of average airborne loss in pendimethalin and dicamba, respectively, compared to 0.56% in napropamide. The amount of airborne losses in turfgrass was highest at one day after application and then a declined trend was observed as the time was prolonged. Key words. Herbicides, Turfgrass field, Bare ground, Volatility.

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